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Gene information
General information
TC008778 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9821333..9823337
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008778
    iBeetle screen iB_08797

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008778 RA
      MTKLVLYLL PLIISCNAI HPGPVVRAT KGEVWPKPQ 
      VEEKTEQYY TVRPHGFNF KGPTNIGCP NLLNDAFTR 
      YWTIIATAS SLERRGRLH EVGRKPKTK FWKADSNYL 
      GDLTNLHVQ LDDCASEYV LPAFGDNEN YTLSVTSEG 
      ASLTADTIW GVLRGLETF SQLIYLEQD TLIINATNV 
      NDYPRFSHR GLLLDTSRH FIPIYIILQ TLDAMSYNK 
      FNVFHWHIT DDHSFPYKS RTYPELSDE GAYHPVSKV 
      YEQSDVSKV IEYARVRGI RVIPEFDTP GHTSSWGAA 
      HPELLTTCY TNDKPDGDL GPMDPTKNS TYDFLTKLF 
      TEVVEVFPD SYFHIGGDE VDFTCWQHN PDIASFMKA 
      NNISTYEDL ESYFIQHVV NLLDSLNSN YLVWEEVFV 
      NGVTLPDST LVHVWRDNG HETLNSVTK AGKYGIFSS 
      CWYLDHVSS GGDWEKFYE CEALDFPGT EEQKKLVLG 
      GEACMWSEA VNEYNVMPR VWPRASAVA EKLWSAGNV 
      NDTQAAKGR LEEHTCRMN NRGIAAQPP NGPGVCL
    CDS sequence
    • >TC008778 RA
      atgaccaaa ttagtttta tatttgctt ccgctaatc 
      atcagctgc aatgctatt catcctggc cccgtggtc 
      agggcaacc aaaggcgaa gtctggcct aaaccacaa 
      gtagaggaa aaaacagag cagtattac acagtacga 
      ccgcacggt ttcaatttt aagggcccc accaacatc 
      ggttgcccg aatcttctg aacgatgcc tttacgaga 
      tactggact attatagcg actgcaagt tcactagaa 
      cgcagaggt cgtctgcat gaagtgggg cgaaaacca 
      aaaaccaaa ttttggaaa gccgacagc aattatctc 
      ggtgattta accaattta catgttcag ttagacgat 
      tgtgctagt gaatatgta cttccagct ttcggggac 
      aatgaaaat tacacactc agtgtcact tcggaggga 
      gcttcactt acggccgac acaatttgg ggggttttg 
      cgtggtttg gagactttt tcccagttg atttatcta 
      gagcaagat actcttatt ataaacgcg actaacgta 
      aatgattat ccgaggttt tctcacaga ggtctttta 
      ttagacacc tccagacac tttataccc atttatatc 
      attcttcaa actttggac gcaatgtcg tacaataaa 
      tttaacgtc ttccattgg catatcaca gatgatcac 
      agttttccc tacaagagt cgtacttat ccagaactg 
      agcgatgaa ggggcctac catccagtt tccaaagtt 
      tacgagcaa agtgatgtt agtaaagtt atagaatat 
      gcacgtgtg agaggcatt cgggttatt ccagagttt 
      gatacccca ggccatact agctcctgg ggagcagct 
      catcctgaa ttattgact acctgctac acaaatgat 
      aaacctgac ggagacttg ggacccatg gacccaact 
      aaaaactcc acttatgat tttttgacc aaattattt 
      actgaagtt gttgaagtt ttcccagat tcttatttc 
      cacattggt ggtgacgaa gttgatttt acttgctgg 
      caacacaat cccgacatt gcaagcttc atgaaagcc 
      aacaatatt tcgacctac gaagacctg gaaagttac 
      ttcatacaa cacgtggtc aatctcctt gattctcta 
      aattcaaat tatttagtg tgggaggaa gttttcgta 
      aatggggtc actcttccc gacagtact ctcgtgcac 
      gtttggaga gacaacggc cacgagacc ttgaacagc 
      gtgaccaaa gcgggaaaa tatggaatt ttttccagt 
      tgttggtac ctggaccat gtgtccagt ggaggcgac 
      tgggaaaag ttctatgag tgtgaagcg cttgatttt 
      cctggtact gaggaacag aaaaaattg gttctggga 
      ggagaagcc tgtatgtgg tccgaagct gtgaacgag 
      tataacgtc atgccaaga gtgtggcca agagctagt 
      gccgttgcg gaaaaactg tggtctgcg ggaaacgtt 
      aacgatact caagcagcc aaaggacgg ctagaggaa 
      cacacttgc aggatgaat aacagggga atcgcggca 
      cagcctcct aatggacca ggagtgtgt ctttaa
    mRNA sequence
    • >TC008778 RA
      tcccaaaaa tagttatct cggcgtatc ttcttcaag 
      tgaatcaac acagattta taaacgaaa acatttgag 
      tttcaacaa gtcagttac tagagtcag ttgtgataa 
      gcgctctgt catgaccaa attagtttt atatttgct 
      tccgctaat catcagctg caatgctat tcatcctgg 
      ccccgtggt cagggcaac caaaggcga agtctggcc 
      taaaccaca agtagagga aaaaacaga gcagtatta 
      cacagtacg accgcacgg tttcaattt taagggccc 
      caccaacat cggttgccc gaatcttct gaacgatgc 
      ctttacgag atactggac tattatagc gactgcaag 
      ttcactaga acgcagagg tcgtctgca tgaagtggg 
      gcgaaaacc aaaaaccaa attttggaa agccgacag 
      caattatct cggtgattt aaccaattt acatgttca 
      gttagacga ttgtgctag tgaatatgt acttccagc 
      tttcgggga caatgaaaa ttacacact cagtgtcac 
      ttcggaggg agcttcact tacggccga cacaatttg 
      gggggtttt gcgtggttt ggagacttt ttcccagtt 
      gatttatct agagcaaga tactcttat tataaacgc 
      gactaacgt aaatgatta tccgaggtt ttctcacag 
      aggtctttt attagacac ctccagaca ctttatacc 
      catttatat cattcttca aactttgga cgcaatgtc 
      gtacaataa atttaacgt cttccattg gcatatcac 
      agatgatca cagttttcc ctacaagag tcgtactta 
      tccagaact gagcgatga aggggccta ccatccagt 
      ttccaaagt ttacgagca aagtgatgt tagtaaagt 
      tatagaata tgcacgtgt gagaggcat tcgggttat 
      tccagagtt tgatacccc aggccatac tagctcctg 
      gggagcagc tcatcctga attattgac tacctgcta 
      cacaaatga taaacctga cggagactt gggacccat 
      ggacccaac taaaaactc cacttatga ttttttgac 
      caaattatt tactgaagt tgttgaagt tttcccaga 
      ttcttattt ccacattgg tggtgacga agttgattt 
      tacttgctg gcaacacaa tcccgacat tgcaagctt 
      catgaaagc caacaatat ttcgaccta cgaagacct 
      ggaaagtta cttcataca acacgtggt caatctcct 
      tgattctct aaattcaaa ttatttagt gtgggagga 
      agttttcgt aaatggggt cactcttcc cgacagtac 
      tctcgtgca cgtttggag agacaacgg ccacgagac 
      cttgaacag cgtgaccaa agcgggaaa atatggaat 
      tttttccag ttgttggta cctggacca tgtgtccag 
      tggaggcga ctgggaaaa gttctatga gtgtgaagc 
      gcttgattt tcctggtac tgaggaaca gaaaaaatt 
      ggttctggg aggagaagc ctgtatgtg gtccgaagc 
      tgtgaacga gtataacgt catgccaag agtgtggcc 
      aagagctag tgccgttgc ggaaaaact gtggtctgc 
      gggaaacgt taacgatac tcaagcagc caaaggacg 
      gctagagga acacacttg caggatgaa taacagggg 
      aatcgcggc acagcctcc taatggacc aggagtgtg 
      tctttaaac cttttccat agcttaata aaaactgcc 
      acaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY