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Gene information
General information
TC010871 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5066614..5071504
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010871
    iBeetle screen iB_08796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010871 RA
      MNGLSFSEL CCLFCCPPC PSRIAAKLA FLPPEPTYE 
FVSDENGKC SFTLTDRAE WQYSEREKE NVEGFFTRT 
NRGNRIACL FVRCSNTAR FTILFSHGN AVDLGQMSS 
FYLGLGSRI NCNIFSYDY SGYGVSAGK PSEKNLYAD 
IDAAWQALR TRYGISPEN IILYGQSIG TVPTVDLAA 
RYEVGAVIL HSPLMSGMR VAFPNTKRT WFFDAFPSI 
DKVPKVMSP TLVIHGTED EVIDFSHGL TIFEKCPRA 
VEPLWVEGA GHNDVELYN VYLERLKQF VNVELIN
    CDS sequence
    • >TC010871 RA
      atgaatggt ttgagtttt agtgagttg tgttgtctg 
ttttgttgt cctccttgt ccgagcaga atagcagca 
aaattggca tttctaccc ccagaacca acctacgaa 
tttgtctcc gacgagaat ggaaagtgc agtttcacg 
ttaaccgat cgcgcagaa tggcaatac tcagaaaga 
gaaaaagaa aacgtcgag ggatttttc acgaggacg 
aaccgcggg aaccgaatc gcctgtctg tttgttaga 
tgtagtaat actgccaga ttcacaatc ctgttttct 
cacggaaac gccgtcgat ctcggacaa atgagcagt 
ttttaccta ggactgggc tcaagaatc aactgcaac 
atatttagt tacgactat tcggggtat ggggtcagt 
gccgggaag ccctccgag aagaacctc tacgccgac 
atcgacgcg gcctggcag gcgctgcgc acccggtac 
gggatcagc cccgaaaac atcatcctt tacggtcaa 
agcatcggc acggtcccg actgtggac cttgccgcc 
aggtacgag gttggggct gtcatcctg cattcgcct 
ctgatgtct ggaatgaga gtggccttc cccaatacc 
aagaggacc tggtttttc gatgccttt cccagtatt 
gataaagtt cccaaagtc atgtcgcca actttagtt 
attcatggt actgaagac gaagttata gacttttcc 
catgggctt accattttt gagaagtgc ccaagagct 
gtcgaacct ttatgggtg gagggcgca ggccacaac 
gacgtggag ttatataac gtgtacctg gaaagacta 
aaacaattc gtaaatgtg gagttaatc aactga
    mRNA sequence
    • >TC010871 RA
      tggtggttg tgtacaagc ctttaaaat ggcgttgtc 
aacaatgag cacctaggt ttcttagat taattgctg 
tataaacag ttattatat tagttttaa aagttaatt 
tagtttagt tagagttgt attaattaa aaatgaatg 
gtttgagtt ttagtgagt tgtgttgtc tgttttgtt 
gtcctcctt gtccgagca gaatagcag caaaattgg 
catttctac ccccagaac caacctacg aatttgtct 
ccgacgaga atggaaagt gcagtttca cgttaaccg 
atcgcgcag aatggcaat actcagaaa gagaaaaag 
aaaacgtcg agggatttt tcacgagga cgaaccgcg 
ggaaccgaa tcgcctgtc tgtttgtta gatgtagta 
atactgcca gattcacaa tcctgtttt ctcacggaa 
acgccgtcg atctcggac aaatgagca gtttttacc 
taggactgg gctcaagaa tcaactgca acatattta 
gttacgact attcggggt atggggtca gtgccggga 
agccctccg agaagaacc tctacgccg acatcgacg 
cggcctggc aggcgctgc gcacccggt acgggatca 
gccccgaaa acatcatcc tttacggtc aaagcatcg 
gcacggtcc cgactgtgg accttgccg ccaggtacg 
aggttgggg ctgtcatcc tgcattcgc ctctgatgt 
ctggaatga gagtggcct tccccaata ccaagagga 
cctggtttt tcgatgcct ttcccagta ttgataaag 
ttcccaaag tcatgtcgc caactttag ttattcatg 
gtactgaag acgaagtta tagactttt cccatgggc 
ttaccattt ttgagaagt gcccaagag ctgtcgaac 
ctttatggg tggagggcg caggccaca acgacgtgg 
agttatata acgtgtacc tggaaagac taaaacaat 
tcgtaaatg tggagttaa tcaactgac agctgctgg 
gcgcggatg actgcacgt ttagttcag gaacctcaa 
gttcggtgg aggagaaac tgctctagc ccggggcag 
cggacttgg tcagccgcc agtccgcaa cgcctctgt 
actgccctt caccacacc tcgccctgc gcgcgcttt 
ccggcattc tggcgcgag ccgaacgtc aatgcaaca 
gcgctcgca gttcgccac attcccgcc aagtggcag 
cggcgccac catccaaag cgcactctc tgccagctt 
gcaacagtg tgccaccag tatgcctta ctgcatgcc 
gctgtatgg ttctcgaaa ctacttgct agcattagt 
gtacatacg atgtggact tttaattaa ataatcgga 
caatctttc aagtgatgc aatattaaa tgtagtacc 
catcaacgt aactctcag tgtaattat acagtgtgg 
actctttct atgatgata taaatataa tgaaaaaag 
tgtttcttt tggtgcatc gggctaatg tttatattc 
ctctcatta ctgttgttt tttatatga ctggatgct 
cggataaca tacaaatgt ccaccaaac atcaaaatt 
aactgtaca aaactcact tttttaata cttcaaacc 
caatgtgta gggtagttg aaccagtga gattcaagt 
tcatatcgc tgcgttctg ttcactgaa gttgttcag 
ttgttttgt ggacatgct gtattttat tattattta 
ttgccctat tatttgtgt tcaataaaa tatcaagtc 
gttcaacat atatttaag acaggtgga ctagttgct 
aggtggggt actcactag ttcgccatt gctctgatt 
attttattg taacataaa ctaaaaata ctttttaat 
acattttta ttaactatg gtaaataat tttgtaagt 
atcgtgttt gatttgttt tatgtaata gtcaattcg 
ttgaaacaa gctttaaat catcaacag cacaacact 
accgaaaat tacatgaag caactcaca catgagaat 
ataggcaat tttttataa aaggaaaat acttggatt 
atattattt aaatatgtg attttttgt gaaattagt 
aacctttga tacatatca tttcgaatt gattttgca 
tttataagc gtaaagtat gtaaggcga cgtttttta 
agtttttgt attcgttct gttgatgag tgcaaaatt 
ttagctgag ttgtcaatt gtccccaaa catctttgc 
aacttttga tttccgaaa aattaaaat aaatatatg 
aaaacagta tttatacga tcacttttt gttttattt 
ctgtaataa gttatcagt acacctgac acaacctga 
cctaattat ttacaagac aataagact tgtattgta 
aacactaaa cctactcat aaaaaataa gaaaatagt 
tttaatttc tagcacgtt ggcgcccta agctcaaac 
taactcgga ggccgcgag cgtgttacc cgaaccatg 
ttttctcat ttttataaa caaatcgtt aataaaaac 
ttgaaacct gc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY