Status
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Gene information
General information
TC010871 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5066614..5071504
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010871
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08796

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010871 RA
      MNGLSFSEL CCLFCCPPC PSRIAAKLA FLPPEPTYE 
      FVSDENGKC SFTLTDRAE WQYSEREKE NVEGFFTRT 
      NRGNRIACL FVRCSNTAR FTILFSHGN AVDLGQMSS 
      FYLGLGSRI NCNIFSYDY SGYGVSAGK PSEKNLYAD 
      IDAAWQALR TRYGISPEN IILYGQSIG TVPTVDLAA 
      RYEVGAVIL HSPLMSGMR VAFPNTKRT WFFDAFPSI 
      DKVPKVMSP TLVIHGTED EVIDFSHGL TIFEKCPRA 
      VEPLWVEGA GHNDVELYN VYLERLKQF VNVELIN
    CDS sequence
    • >TC010871 RA
      atgaatggt ttgagtttt agtgagttg tgttgtctg 
      ttttgttgt cctccttgt ccgagcaga atagcagca 
      aaattggca tttctaccc ccagaacca acctacgaa 
      tttgtctcc gacgagaat ggaaagtgc agtttcacg 
      ttaaccgat cgcgcagaa tggcaatac tcagaaaga 
      gaaaaagaa aacgtcgag ggatttttc acgaggacg 
      aaccgcggg aaccgaatc gcctgtctg tttgttaga 
      tgtagtaat actgccaga ttcacaatc ctgttttct 
      cacggaaac gccgtcgat ctcggacaa atgagcagt 
      ttttaccta ggactgggc tcaagaatc aactgcaac 
      atatttagt tacgactat tcggggtat ggggtcagt 
      gccgggaag ccctccgag aagaacctc tacgccgac 
      atcgacgcg gcctggcag gcgctgcgc acccggtac 
      gggatcagc cccgaaaac atcatcctt tacggtcaa 
      agcatcggc acggtcccg actgtggac cttgccgcc 
      aggtacgag gttggggct gtcatcctg cattcgcct 
      ctgatgtct ggaatgaga gtggccttc cccaatacc 
      aagaggacc tggtttttc gatgccttt cccagtatt 
      gataaagtt cccaaagtc atgtcgcca actttagtt 
      attcatggt actgaagac gaagttata gacttttcc 
      catgggctt accattttt gagaagtgc ccaagagct 
      gtcgaacct ttatgggtg gagggcgca ggccacaac 
      gacgtggag ttatataac gtgtacctg gaaagacta 
      aaacaattc gtaaatgtg gagttaatc aactga
    mRNA sequence
    • >TC010871 RA
      tggtggttg tgtacaagc ctttaaaat ggcgttgtc 
      aacaatgag cacctaggt ttcttagat taattgctg 
      tataaacag ttattatat tagttttaa aagttaatt 
      tagtttagt tagagttgt attaattaa aaatgaatg 
      gtttgagtt ttagtgagt tgtgttgtc tgttttgtt 
      gtcctcctt gtccgagca gaatagcag caaaattgg 
      catttctac ccccagaac caacctacg aatttgtct 
      ccgacgaga atggaaagt gcagtttca cgttaaccg 
      atcgcgcag aatggcaat actcagaaa gagaaaaag 
      aaaacgtcg agggatttt tcacgagga cgaaccgcg 
      ggaaccgaa tcgcctgtc tgtttgtta gatgtagta 
      atactgcca gattcacaa tcctgtttt ctcacggaa 
      acgccgtcg atctcggac aaatgagca gtttttacc 
      taggactgg gctcaagaa tcaactgca acatattta 
      gttacgact attcggggt atggggtca gtgccggga 
      agccctccg agaagaacc tctacgccg acatcgacg 
      cggcctggc aggcgctgc gcacccggt acgggatca 
      gccccgaaa acatcatcc tttacggtc aaagcatcg 
      gcacggtcc cgactgtgg accttgccg ccaggtacg 
      aggttgggg ctgtcatcc tgcattcgc ctctgatgt 
      ctggaatga gagtggcct tccccaata ccaagagga 
      cctggtttt tcgatgcct ttcccagta ttgataaag 
      ttcccaaag tcatgtcgc caactttag ttattcatg 
      gtactgaag acgaagtta tagactttt cccatgggc 
      ttaccattt ttgagaagt gcccaagag ctgtcgaac 
      ctttatggg tggagggcg caggccaca acgacgtgg 
      agttatata acgtgtacc tggaaagac taaaacaat 
      tcgtaaatg tggagttaa tcaactgac agctgctgg 
      gcgcggatg actgcacgt ttagttcag gaacctcaa 
      gttcggtgg aggagaaac tgctctagc ccggggcag 
      cggacttgg tcagccgcc agtccgcaa cgcctctgt 
      actgccctt caccacacc tcgccctgc gcgcgcttt 
      ccggcattc tggcgcgag ccgaacgtc aatgcaaca 
      gcgctcgca gttcgccac attcccgcc aagtggcag 
      cggcgccac catccaaag cgcactctc tgccagctt 
      gcaacagtg tgccaccag tatgcctta ctgcatgcc 
      gctgtatgg ttctcgaaa ctacttgct agcattagt 
      gtacatacg atgtggact tttaattaa ataatcgga 
      caatctttc aagtgatgc aatattaaa tgtagtacc 
      catcaacgt aactctcag tgtaattat acagtgtgg 
      actctttct atgatgata taaatataa tgaaaaaag 
      tgtttcttt tggtgcatc gggctaatg tttatattc 
      ctctcatta ctgttgttt tttatatga ctggatgct 
      cggataaca tacaaatgt ccaccaaac atcaaaatt 
      aactgtaca aaactcact tttttaata cttcaaacc 
      caatgtgta gggtagttg aaccagtga gattcaagt 
      tcatatcgc tgcgttctg ttcactgaa gttgttcag 
      ttgttttgt ggacatgct gtattttat tattattta 
      ttgccctat tatttgtgt tcaataaaa tatcaagtc 
      gttcaacat atatttaag acaggtgga ctagttgct 
      aggtggggt actcactag ttcgccatt gctctgatt 
      attttattg taacataaa ctaaaaata ctttttaat 
      acattttta ttaactatg gtaaataat tttgtaagt 
      atcgtgttt gatttgttt tatgtaata gtcaattcg 
      ttgaaacaa gctttaaat catcaacag cacaacact 
      accgaaaat tacatgaag caactcaca catgagaat 
      ataggcaat tttttataa aaggaaaat acttggatt 
      atattattt aaatatgtg attttttgt gaaattagt 
      aacctttga tacatatca tttcgaatt gattttgca 
      tttataagc gtaaagtat gtaaggcga cgtttttta 
      agtttttgt attcgttct gttgatgag tgcaaaatt 
      ttagctgag ttgtcaatt gtccccaaa catctttgc 
      aacttttga tttccgaaa aattaaaat aaatatatg 
      aaaacagta tttatacga tcacttttt gttttattt 
      ctgtaataa gttatcagt acacctgac acaacctga 
      cctaattat ttacaagac aataagact tgtattgta 
      aacactaaa cctactcat aaaaaataa gaaaatagt 
      tttaatttc tagcacgtt ggcgcccta agctcaaac 
      taactcgga ggccgcgag cgtgttacc cgaaccatg 
      ttttctcat ttttataaa caaatcgtt aataaaaac 
      ttgaaacct gc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY