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Gene information
General information
TC008787 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9869973..9876380
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008787
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008787 RA
      MSPFVVLFV FSHLTFIVT TNEVIYVNN RRLLNVIPP 
      QSDGKKVEA SKAEEPKDH VETVHIKNE AKVEPQPVK 
      PQEVPPEPK KPSKTLEKN ETKVEDHKV FKPTEMESG 
      ALLRGVSVV VILTALVIV YMALKTYCG KKSKQPVMV 
      SKYGVRTRR SDIEMTPLP LDEDEEDET LFDESDPAS 
      TLRRRKLVE CAQEKIEQA SELLKKKIP CAGHFLAPK 
      RLWLKRIST PNCDVVVVF PPGAEDSVL LWLLSKLRQ 
      SPGLTVHVR HHASTQSSA FYLTAPLSI LLKAAEEYH 
      LPKILKESK GGGLKEFLV QDFQSYEGS DAEDHFFTT 
      EERQWLVLR LLESIRAKP NDSQAIPGL TLLEGQPIV 
      PKCLTSGII SQVFPLHEP AALERLQNT WVRDFCARQ 
      PLDEITEYF GVKIGMYFA WLGHYTTAL SIPAIVGFF 
      FWLCCNGKH QTLEDVGYV LFSVFNVVW VTTYLQAWK 
      RYSAELAFR WGTLDQRDD LLAEPRPLF RGPLQPSPV 
      TGRLEPWHP TWKRHVFRY CISVPIIVI CLSTVFFVM 
      IVSLQIQDW WDGLLSARG LPMWLGYLP KIMLAVVIS 
      LMDEAYFKI AIWLNDKEN YRLETKYEN HLIGKVALF 
      QFVNSFLSL FYIAFYLQD QARLKEQLA ALLISRQVI 
      GNLKESALP YMLEHLRLA KMSFDLWGA LSPQNARPP 
      PGEETEKVP EIKEEPSTE QANKRSMSQ AELESSLYK 
      YDGTFADHL EMTMQLGYV ILFSSAFPP AAVCAMLNN 
      LIEIRSDAF KLAYVCQRP FGQRVPNIG TWQNCMEYM 
      SIMAVLVNC ALIGLSGQV HRMFPDMTA TQTILLIVA 
      LEHIMLVIR FIITCAIPD IPGWLATEM AKIEWARRE 
      ASRLANTST PSPEELSAK IVGRFSVSP SHSLVAGNE 
      NKLDIKLRE MSETLKETT HSQNVTPVP TPTSPAPGT 
      AHYKPVSIT PDSIQQIPP FKPRKSKEW TPAEVVPPE 
      QTHHLTIGP GGGAEWAKR LKEESDIHR STDCITPKD 
      ASSSDSDLL KSSPVWPPR PRSPPQPPN RARPAPADS 
      SLSDSARSV SSQEAADEA ARAAEELAA KKTRVKQSL 
      MKRARSVAI FSLKLKERR AREAQEKAA NKPVTPTAP 
      LPPPQCGGG ELSCIPIEK LIQLEDVRR NVRPNQTGT 
      L
    CDS sequence
    • >TC008787 RA
      atgtctcct tttgttgtt ttgtttgtg ttttcgcac 
      ctaaccttt attgtaaca actaatgaa gtgatttat 
      gttaataac cggcgtttg ctgaatgtg ataccccct 
      cagagtgac ggaaaaaaa gtggaagct agcaaagca 
      gaagagcct aaggatcat gtagaaaca gtgcacata 
      aaaaatgaa gcaaaggtt gaaccccaa cctgtcaaa 
      cctcaggaa gtacctcct gaaccaaaa aaaccgagc 
      aaaacactc gaaaaaaat gaaacgaaa gttgaagat 
      cacaaagtt ttcaaacca acggaaatg gaatcgggt 
      gcccttttg cggggtgtt tccgttgtg gtgatttta 
      acagctcta gtaattgtt tacatggcc ttgaagact 
      tattgcggt aaaaaaagc aaacaacct gtgatggta 
      agcaaatac ggtgtgagg acacggcgc agtgacata 
      gagatgaca cctcttcca cttgacgag gacgaagaa 
      gacgagact ttgtttgat gaatccgac cctgctagc 
      accctccgg cgccgcaag ttggtcgag tgcgcccaa 
      gaaaaaatc gaacaagcc tcggaactg ctaaaaaag 
      aagatcccg tgcgccggc cacttcctg gccccgaag 
      cgcctctgg ttgaagcga atttcgacg ccgaactgc 
      gatgttgtt gttgttttc ccgcccggc gccgaggac 
      tcggtgttg ttgtggctc ctgtccaag ctgagacag 
      tcgccgggg cttacggta cacgtaaga caccatgct 
      tccacccaa agcagcgca ttttacctc acggcacct 
      ctctcgatt ttgctcaaa gctgccgaa gaatatcat 
      cttcccaaa atcttaaag gagagcaag ggaggtggc 
      ttgaaggag tttctagtt caggacttc cagtcttat 
      gagggatca gacgctgag gaccacttt tttaccact 
      gaggagaga cagtggctg gttttgagg cttttggag 
      agcataagg gccaaaccc aacgattcc caggcaatt 
      ccagggttg acacttctc gaaggacag cccattgtc 
      cccaaatgt ttaacttct ggaataatc tcgcaggta 
      tttcccctg cacgaaccc gcggctctg gaacgcctc 
      cagaacacg tgggtccga gacttttgt gcccggcag 
      cccctagac gaaataacc gaatatttt ggggtcaaa 
      atcgggatg tactttgcc tggctgggt cattacacc 
      acggctttg agcattccg gcgattgtg ggatttttc 
      ttttggtta tgttgtaac ggcaaacac cagacgctg 
      gaagacgtg ggctatgtc ctgttttca gttttcaac 
      gtagtttgg gtcacgact tacctccaa gcttggaag 
      cgttattcg gccgagttg gccttcagg tggggcact 
      ctggatcaa cgcgatgac ctcctagcc gagccccgc 
      ccgctgttc aggggacct ctccagcct agtccggtg 
      accggccgc ttggagccc tggcacccg acgtggaag 
      agacacgtt ttcagatat tgcatttcg gtgccgatc 
      attgtcatt tgtttatcg accgttttc ttcgtcatg 
      atagtttcg ttgcaaatt caggattgg tgggacggg 
      ttgctaagt gcgagagga ctgcccatg tggctgggg 
      tacctcccc aaaatcatg ttggctgtc gttatctcg 
      ctaatggac gaggcctac ttcaaaata gccatctgg 
      ctcaacgat aaagagaac taccgtctc gagaccaag 
      tacgaaaac cacctcatc ggcaaagtg gccttgttt 
      caatttgtc aattcgttc ctctcatta ttttacatc 
      gcattctat ttgcaagat caggcgcgc ctcaaagag 
      caattggcc gctttactg atatctcga caagtgatc 
      ggtaatcta aaagaatcg gccttgcct tacatgctt 
      gagcacttg cgattggcc aaaatgagt ttcgatctg 
      tggggcgct ttaagtccc caaaacgcc agaccgcca 
      cctggcgag gaaaccgag aaagtacca gaaattaaa 
      gaagaaccg agcaccgaa caagccaac aaacggagc 
      atgagtcaa gccgaactc gaaagttct ttatacaaa 
      tacgatgga actttcgcc gatcatttg gaaatgaca 
      atgcagtta ggatatgtc attttgttc tcgtctgcc 
      tttcctccg gctgcggtt tgcgcgatg ttgaataac 
      ttgatcgaa atcaggagt gatgccttc aagctggct 
      tatgtgtgc caaaggccg tttgggcaa agagtgcca 
      aatatcggc acgtggcag aattgtatg gaatatatg 
      agtataatg gcggttttg gtcaactgc gctctgatt 
      ggtttatcg gggcaagtg caccgaatg tttcccgac 
      atgactgcc actcagaca attctgttg attgtggcc 
      ttggagcat atcatgctt gttattaga ttcatcatc 
      acgtgtgcc atcccggac atccccggt tggttggcc 
      accgaaatg gccaaaatc gagtgggct cgacgcgaa 
      gctagccga ttggccaac acgtcgacg ccctcaccc 
      gaagagcta tcggccaag atcgtcggg agattttca 
      gtgtcgccg agccacagt ctggtcgcc ggcaacgaa 
      aacaaactc gacattaag ttgcgcgag atgagcgaa 
      actttgaag gaaacgaca cattcgcaa aatgtcaca 
      ccggtaccg acccccact tcgccagcg ccgggcacg 
      gcccattac aaacccgtc tccataacc cccgatagc 
      attcaacaa attcccccg tttaagcca agaaaatcc 
      aaggagtgg accccagct gaggtggtg ccgcccgaa 
      cagacccac catttgacg atcgggcct ggagggggc 
      gccgagtgg gccaaacgc ctcaaagag gaatccgac 
      atacatcga agtactgac tgcattacc cctaaagac 
      gcctctagt tctgattca gacctgttg aaaagtagc 
      ccggtgtgg ccgccgcgc ccgcggtcc ccgccccag 
      ccgcccaac cgggcacgc cccgccccc gccgactcc 
      tccctcagc gacagcgct cgcagcgtg tcgagtcag 
      gaggcggcg gacgaggcg gcccgcgcg gccgaagaa 
      ctagcagcg aagaagacg agagtcaag caaagccta 
      atgaaacga gcccgttct gtggccatt ttctcgctg 
      aagttgaag gagcgtcgc gctagggag gcgcaggag 
      aaggcggcg aacaagccg gtgactccg actgcgccg 
      ttgccgccg ccgcagtgt ggaggcggc gagttgtcg 
      tgcataccc atcgaaaag ctcattcaa ctggaggat 
      gtgagacgc aacgtacgg cctaaccaa accggaact 
      ttgtag
    mRNA sequence
    • >TC008787 RA
      aaattgttt taattgata aaacgagtt aattgttgt 
      gtttgccct attcaaact ggttaatcg tgttttgcg 
      tttataaaa gcaacaaat tttaaaaaa ctgtacatt 
      ttgacaggt gacgttact gttgtcaat atgctggaa 
      gaaaaaata caaaataat gattaaacc ccgtaaaag 
      aagtgaaat gtgattaaa aaatctgca agtttttga 
      tgcgttttt gtggttaat ttatccaat gtctccttt 
      tgttgtttt gtttgtgtt ttcgcacct aacctttat 
      tgtaacaac taatgaagt gatttatgt taataaccg 
      gcgtttgct gaatgtgat accccctca gagtgacgg 
      aaaaaaagt ggaagctag caaagcaga agagcctaa 
      ggatcatgt agaaacagt gcacataaa aaatgaagc 
      aaaggttga accccaacc tgtcaaacc tcaggaagt 
      acctcctga accaaaaaa accgagcaa aacactcga 
      aaaaaatga aacgaaagt tgaagatca caaagtttt 
      caaaccaac ggaaatgga atcgggtgc ccttttgcg 
      gggtgtttc cgttgtggt gattttaac agctctagt 
      aattgttta catggcctt gaagactta ttgcggtaa 
      aaaaagcaa acaacctgt gatggtaag caaatacgg 
      tgtgaggac acggcgcag tgacataga gatgacacc 
      tcttccact tgacgagga cgaagaaga cgagacttt 
      gtttgatga atccgaccc tgctagcac cctccggcg 
      ccgcaagtt ggtcgagtg cgcccaaga aaaaatcga 
      acaagcctc ggaactgct aaaaaagaa gatcccgtg 
      cgccggcca cttcctggc cccgaagcg cctctggtt 
      gaagcgaat ttcgacgcc gaactgcga tgttgttgt 
      tgttttccc gcccggcgc cgaggactc ggtgttgtt 
      gtggctcct gtccaagct gagacagtc gccggggct 
      tacggtaca cgtaagaca ccatgcttc cacccaaag 
      cagcgcatt ttacctcac ggcacctct ctcgatttt 
      gctcaaagc tgccgaaga atatcatct tcccaaaat 
      cttaaagga gagcaaggg aggtggctt gaaggagtt 
      tctagttca ggacttcca gtcttatga gggatcaga 
      cgctgagga ccacttttt taccactga ggagagaca 
      gtggctggt tttgaggct tttggagag cataagggc 
      caaacccaa cgattccca ggcaattcc agggttgac 
      acttctcga aggacagcc cattgtccc caaatgttt 
      aacttctgg aataatctc gcaggtatt tcccctgca 
      cgaacccgc ggctctgga acgcctcca gaacacgtg 
      ggtccgaga cttttgtgc ccggcagcc cctagacga 
      aataaccga atattttgg ggtcaaaat cgggatgta 
      ctttgcctg gctgggtca ttacaccac ggctttgag 
      cattccggc gattgtggg atttttctt ttggttatg 
      ttgtaacgg caaacacca gacgctgga agacgtggg 
      ctatgtcct gttttcagt tttcaacgt agtttgggt 
      cacgactta cctccaagc ttggaagcg ttattcggc 
      cgagttggc cttcaggtg gggcactct ggatcaacg 
      cgatgacct cctagccga gccccgccc gctgttcag 
      gggacctct ccagcctag tccggtgac cggccgctt 
      ggagccctg gcacccgac gtggaagag acacgtttt 
      cagatattg catttcggt gccgatcat tgtcatttg 
      tttatcgac cgttttctt cgtcatgat agtttcgtt 
      gcaaattca ggattggtg ggacgggtt gctaagtgc 
      gagaggact gcccatgtg gctggggta cctccccaa 
      aatcatgtt ggctgtcgt tatctcgct aatggacga 
      ggcctactt caaaatagc catctggct caacgataa 
      agagaacta ccgtctcga gaccaagta cgaaaacca 
      cctcatcgg caaagtggc cttgtttca atttgtcaa 
      ttcgttcct ctcattatt ttacatcgc attctattt 
      gcaagatca ggcgcgcct caaagagca attggccgc 
      tttactgat atctcgaca agtgatcgg taatctaaa 
      agaatcggc cttgcctta catgcttga gcacttgcg 
      attggccaa aatgagttt cgatctgtg gggcgcttt 
      aagtcccca aaacgccag accgccacc tggcgagga 
      aaccgagaa agtaccaga aattaaaga agaaccgag 
      caccgaaca agccaacaa acggagcat gagtcaagc 
      cgaactcga aagttcttt atacaaata cgatggaac 
      tttcgccga tcatttgga aatgacaat gcagttagg 
      atatgtcat tttgttctc gtctgcctt tcctccggc 
      tgcggtttg cgcgatgtt gaataactt gatcgaaat 
      caggagtga tgccttcaa gctggctta tgtgtgcca 
      aaggccgtt tgggcaaag agtgccaaa tatcggcac 
      gtggcagaa ttgtatgga atatatgag tataatggc 
      ggttttggt caactgcgc tctgattgg tttatcggg 
      gcaagtgca ccgaatgtt tcccgacat gactgccac 
      tcagacaat tctgttgat tgtggcctt ggagcatat 
      catgcttgt tattagatt catcatcac gtgtgccat 
      cccggacat ccccggttg gttggccac cgaaatggc 
      caaaatcga gtgggctcg acgcgaagc tagccgatt 
      ggccaacac gtcgacgcc ctcacccga agagctatc 
      ggccaagat cgtcgggag attttcagt gtcgccgag 
      ccacagtct ggtcgccgg caacgaaaa caaactcga 
      cattaagtt gcgcgagat gagcgaaac tttgaagga 
      aacgacaca ttcgcaaaa tgtcacacc ggtaccgac 
      ccccacttc gccagcgcc gggcacggc ccattacaa 
      acccgtctc cataacccc cgatagcat tcaacaaat 
      tcccccgtt taagccaag aaaatccaa ggagtggac 
      cccagctga ggtggtgcc gcccgaaca gacccacca 
      tttgacgat cgggcctgg agggggcgc cgagtgggc 
      caaacgcct caaagagga atccgacat acatcgaag 
      tactgactg cattacccc taaagacgc ctctagttc 
      tgattcaga cctgttgaa aagtagccc ggtgtggcc 
      gccgcgccc gcggtcccc gccccagcc gcccaaccg 
      ggcacgccc cgcccccgc cgactcctc cctcagcga 
      cagcgctcg cagcgtgtc gagtcagga ggcggcgga 
      cgaggcggc ccgcgcggc cgaagaact agcagcgaa 
      gaagacgag agtcaagca aagcctaat gaaacgagc 
      ccgttctgt ggccatttt ctcgctgaa gttgaagga 
      gcgtcgcgc tagggaggc gcaggagaa ggcggcgaa 
      caagccggt gactccgac tgcgccgtt gccgccgcc 
      gcagtgtgg aggcggcga gttgtcgtg catacccat 
      cgaaaagct cattcaact ggaggatgt gagacgcaa 
      cgtacggcc taaccaaac cggaacttt gtagcaact 
      ttgctcgca ttcctccta ctcagtcgt cgtgtaagc 
      attctttat ttaagaaat gaaaagtag aaatttgtt 
      ttatcgaca cgaacttgc atgtttttc aatttcaat 
      cgggtgaaa gagtattcc gtgaatcga gcaataata 
      gtttaaaac gtttaaaaa tcaaacaga ttgcaatgc 
      ccgtttatg aaacgatgg taagctgat ttttagata 
      tttaaactt taatgttaa tagttacat aagattgtt 
      tttaaatta ttttttata atctgtaga ttgtatgtt 
      atcctcacg tagcggtaa gactgtaac aattaataa 
      ttagtgaat aggtcggct ggagttttg cttggttgc 
      gtagccgct gtagtttcg ttgggttca ttgtagacc 
      ccctaatcc ctggagtta ttagtttct atattttga 
      ccacgttta gagtaaagt tcgtccagt atattgtaa 
      tgcattgcc ttttcatgc ggctagtgt gaccccacg 
      aagtgtaga aaatgacga ccggagcaa ccctggcct 
      ccacctagt gtttgttta gtctgccgt ccgctttgt 
      cccggctaa cgcctctaa ttaacgaac ccgtctaaa 
      gccggtcgc cactttcta ccagttata gtacactag 
      gccgagtat ttttttgac gttttacat agcgcttat 
      caagtctca atagtggaa ctaacaata agactgcaa 
      atatttttg agatttagt acgaaaaaa tgccttggt 
      attattgtt attagttcg atttttagc cagatttta 
      acgatgtga ttatccaca gcattaaaa acaaacaaa 
      aaaattgac aaattctgt gcttgtcga actttcatg 
      ttgcttaaa tgttaagct tgttgacta tgtatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY