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Gene information
General information
TC008796 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9990728..9992147
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008796
    iBeetle screen iB_08790

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008796 RA
      MALYLSRFY VSSSRFISL LTLTRVLPQ FYSKFEADV 
      IDDFTTQLM QSKISKWES KKRQNQALL ENIPKSDDT 
      HLGEISVEN MGSDQLGFL FQNAIDSND TKAVKNLIN 
      LCIQHNKSP TVNTIVNVL SICGASGDK ETISQIQEL 
      CKTHHPTAL EDNSNFRHY LAKAIWTKG DISKALEMF 
      ENVYRENPF LRRSIRLML KYLISDLIT NGSEAALAN 
      TIAFSERIH RDFQDVSPL TCVWQACFL SEWFTDQSV 
      ALELLEKHN GLCKSVINR IPYVVFVSL KCHRTEVVY 
      RLLEILLKF KMKQQYSNV LESLLEYQF RYGDWNQCS 
      EIIRWAVDN DVALPKTHH IKLINSKLN KKMKTPFAQ 
      LEQESGEKP KWKL
    CDS sequence
    • >TC008796 RA
      atggcactt tatttaagc cgcttttac gtgagcagc 
      tctcgattt atatcactg ctaacttta actcgggtt 
      ttgcctcaa ttttattca aaatttgag gccgatgtt 
      attgacgac tttacaacc cagttgatg caaagtaaa 
      atcagtaaa tgggagtca aagaagcga caaaaccaa 
      gccctgttg gagaacatc cctaaaagt gatgacacc 
      catcttggg gaaatatcg gtggagaat atgggaagt 
      gatcaactt ggctttctc ttccaaaac gcaatagat 
      agtaacgac acaaaagct gttaagaat ttaatcaac 
      ttgtgtatt caacacaat aaatcgccg acagttaat 
      accatagtt aatgtcctt tcaatatgt ggggcaagt 
      ggcgataaa gagaccatt tctcaaatt caggagtta 
      tgcaaaact catcacccc actgcactc gaggacaat 
      tcgaatttt cgccactat ctcgccaag gcaatatgg 
      acgaaagga gatatttcc aaagcctta gaaatgttt 
      gaaaatgtt taccgtgaa aatcccttt ttgaggcga 
      agtatccgc ctcatgtta aaatactta atcagtgat 
      ttgattaca aatggcagt gaagccgca ctagcaaac 
      acgattgct ttctcagaa cggattcat agggacttt 
      caagatgtg agtcccctc acttgcgtg tggcaagcg 
      tgtttcctg agcgagtgg ttcactgat caaagtgta 
      gctttagaa ctactcgaa aaacacaac ggtttgtgc 
      aaatctgtg atcaataga atcccttat gtagttttt 
      gtctcgcta aagtgccac aggaccgaa gttgtctac 
      cggttgttg gaaatttta ctcaagttt aagatgaaa 
      caacaatat tccaacgtg ttggagtct ctgttagaa 
      tatcaattt aggtatgga gattggaat cagtgctcg 
      gaaattatt aggtgggca gttgacaac gacgtggct 
      ttacctaaa acacaccac attaaatta attaattcg 
      aagcttaac aaaaagatg aagactcct ttcgcacag 
      ctggaacag gaatcgggc gagaaacca aagtggaag 
      ttgtaa
    mRNA sequence
    • >TC008796 RA
      tgttcatgg tgccagcac catttttga cacatgacg 
      gcaaatgtc aaaacttaa acaatttca ataataaat 
      aatagtggc aaatcaatt attatgcaa cggttaatg 
      aataaatag tgttgaaaa cgatgtgag tgctagtac 
      agttctctt cgtcgcatg ctagtggtt accactttt 
      tttcttgag gttatgtaa aatccaact tcgagatat 
      ggcacttta tttaagccg cttttacgt gagcagctc 
      tcgatttat atcactgct aactttaac tcgggtttt 
      gcctcaatt ttattcaaa atttgaggc cgatgttat 
      tgacgactt tacaaccca gttgatgca aagtaaaat 
      cagtaaatg ggagtcaaa gaagcgaca aaaccaagc 
      cctgttgga gaacatccc taaaagtga tgacaccca 
      tcttgggga aatatcggt ggagaatat gggaagtga 
      tcaacttgg ctttctctt ccaaaacgc aatagatag 
      taacgacac aaaagctgt taagaattt aatcaactt 
      gtgtattca acacaataa atcgccgac agttaatac 
      catagttaa tgtcctttc aatatgtgg ggcaagtgg 
      cgataaaga gaccatttc tcaaattca ggagttatg 
      caaaactca tcaccccac tgcactcga ggacaattc 
      gaattttcg ccactatct cgccaaggc aatatggac 
      gaaaggaga tatttccaa agccttaga aatgtttga 
      aaatgttta ccgtgaaaa tcccttttt gaggcgaag 
      tatccgcct catgttaaa atacttaat cagtgattt 
      gattacaaa tggcagtga agccgcact agcaaacac 
      gattgcttt ctcagaacg gattcatag ggactttca 
      agatgtgag tcccctcac ttgcgtgtg gcaagcgtg 
      tttcctgag cgagtggtt cactgatca aagtgtagc 
      tttagaact actcgaaaa acacaacgg tttgtgcaa 
      atctgtgat caatagaat cccttatgt agtttttgt 
      ctcgctaaa gtgccacag gaccgaagt tgtctaccg 
      gttgttgga aattttact caagtttaa gatgaaaca 
      acaatattc caacgtgtt ggagtctct gttagaata 
      tcaatttag gtatggaga ttggaatca gtgctcgga 
      aattattag gtgggcagt tgacaacga cgtggcttt 
      acctaaaac acaccacat taaattaat taattcgaa 
      gcttaacaa aaagatgaa gactccttt cgcacagct 
      ggaacagga atcgggcga gaaaccaaa gtggaagtt 
      gtaaatagc aaggaataa taaaacaga ttttaaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY