Status
Please wait ...
Gene information
General information
TC033797
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008815,TC008816
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10339022..10367017
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033797
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08784

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033797 RA
      MRGRMEDPP KVELHLGST LNPEDIKEG DDVYFECII 
      KANPKQHKI TWFHDGTLV SQNMSSGVI ISTHSLVLQ 
      RVTRWQSGA YTCLAANPR GENVSNPVM LRVRYAPVC 
      RDTEMAVIG ASLNEVIKV RCHVSADPS DVTFVWQFN 
      NSGESFDVS PARISASIG NVSELMYTT SSQRDYGTL 
      TCWGKNSIG RQAEPCVFQ VVPAAKPSP LTNCTLRAA 
      TNHSSDVLE VECRAGYDG GLPQRFILE AYDAYTMRL 
      RLNLSSAES DVPVFRLDL GEMLPPSGD GFPPSLRIV 
      VYAQNAKGR SEKLVLEDI MLNDAEKRT DGSSNMSIL 
      PIAALLTGS LLTLGIAVL LIVVLAVRR KHRHCGGGH 
      CSYQLDPSK QPKVPGQSS RPGSMLEIN TGDNRYVVA 
      YTLKPAADC GTQPSANSS VDRQPDILN TPRGADTTT 
      PPGLPRPDA LFTPSTGER NRQQHSPPT FPNFSHEEQ 
      ISSPEVSVS QTGGTLGRP RPRPRDISY SNFATMRRD 
      HIIADAIPG PESCV
    CDS sequence
    • >TC033797 RA
      atgcgcggt cggatggag gatcccccc aaagtggag 
      cttcacctg ggcagcacc ctcaacccc gaagacatc 
      aaagaaggc gacgacgtg tacttcgag tgcatcatc 
      aaagccaac cccaagcag cacaagatc acctggttc 
      cacgacggc acgctggtg agtcagaac atgtcatcg 
      ggcgtgatc atcagcaca cacagcctg gtcctgcag 
      agggtcacg aggtggcag agcggcgcc tacacctgc 
      ttggcggcc aacccccgc ggggagaac gtcagcaat 
      cctgtcatg ctaagggtt agatatgct ccggtttgc 
      cgcgacacc gagatggct gtaatcggg gcctccctc 
      aacgaggtg atcaaagtg cgttgtcac gtgtcggcc 
      gaccccagc gacgtaacc tttgtctgg cagttcaac 
      aacagcggc gagagcttc gacgtgtct cctgcccgc 
      atctcggcc tccatcggc aacgtgagc gagctcatg 
      tacaccacc tcctcccag cgcgactac ggcaccctc 
      acctgctgg ggcaagaac tcgatcggg cgccaggcg 
      gagccctgc gtcttccaa gtggtcccc gcagccaag 
      cccagcccc ctcaccaac tgcaccctg cgcgccgcc 
      accaaccac agctcggac gtgctggag gtggagtgc 
      cgcgccggc tacgacggc ggcctgccc cagcgcttc 
      atcctggag gcgtacgac gcctacacc atgcgcctg 
      cggctcaac ctcagcagc gccgagtcc gacgtcccc 
      gtcttccgg ctggacctg ggggagatg ctgccgccc 
      tccggggac ggcttcccg ccctcgctc cgcatcgtc 
      gtctacgcg cagaacgcg aagggccgc agcgagaag 
      ctcgtcctc gaggacatc atgctcaac gacgccgag 
      aaacgcaca gatgggagc agcaatatg agcatcctg 
      cccattgcg gccctcctg acggggtcg ctcctgact 
      ctgggcatc gccgtgttg ctcatcgtc gtgctggcc 
      gtccgccgg aagcaccgg cactgcggc ggtggccac 
      tgcagctac cagctggac ccctcgaag cagcccaaa 
      gttccgggc cagtcctcc aggcccgga tcgatgctg 
      gaaatcaac acgggagat aatcgctac gtggtggcg 
      tacaccctg aaaccggca gcggactgc ggcacgcag 
      ccctccgcc aattcgtcc gtagatagg caaccagat 
      attctcaac acccccaga ggtgctgac acgacaacg 
      ccacccggg ctgccgaga cctgatgcc cttttcacc 
      ccctcaacg ggagaacgg aatcggcag cagcacagc 
      cccccgaca ttccccaac tttagccac gaagagcag 
      atctcgtcg ccggaagtg tccgtgagc cagacgggg 
      ggcacttta gggcggccc cgtccgcgc cctcgagac 
      atttcctat tccaacttc gccacaatg aggagagac 
      cacatcata gccgacgcc attccggga cctgaaagt 
      tgtgtttaa
    mRNA sequence
    • >TC033797 RA
      gagtaattt tacgctcac attttcgaa agagaatat 
      tttattacg tcttattta gcaacaagc aagcccgcg 
      gttctttaa tatgcgcgg tcggatgga ggatccccc 
      caaagtgga gcttcacct gggcagcac cctcaaccc 
      cgaagacat caaagaagg cgacgacgt gtacttcga 
      gtgcatcat caaagccaa ccccaagca gcacaagat 
      cacctggtt ccacgacgg cacgctggt gagtcagaa 
      catgtcatc gggcgtgat catcagcac acacagcct 
      ggtcctgca gagggtcac gaggtggca gagcggcgc 
      ctacacctg cttggcggc caacccccg cggggagaa 
      cgtcagcaa tcctgtcat gctaagggt tagatatgc 
      tccggtttg ccgcgacac cgagatggc tgtaatcgg 
      ggcctccct caacgaggt gatcaaagt gcgttgtca 
      cgtgtcggc cgaccccag cgacgtaac ctttgtctg 
      gcagttcaa caacagcgg cgagagctt cgacgtgtc 
      tcctgcccg catctcggc ctccatcgg caacgtgag 
      cgagctcat gtacaccac ctcctccca gcgcgacta 
      cggcaccct cacctgctg gggcaagaa ctcgatcgg 
      gcgccaggc ggagccctg cgtcttcca agtggtccc 
      cgcagccaa gcccagccc cctcaccaa ctgcaccct 
      gcgcgccgc caccaacca cagctcgga cgtgctgga 
      ggtggagtg ccgcgccgg ctacgacgg cggcctgcc 
      ccagcgctt catcctgga ggcgtacga cgcctacac 
      catgcgcct gcggctcaa cctcagcag cgccgagtc 
      cgacgtccc cgtcttccg gctggacct gggggagat 
      gctgccgcc ctccgggga cggcttccc gccctcgct 
      ccgcatcgt cgtctacgc gcagaacgc gaagggccg 
      cagcgagaa gctcgtcct cgaggacat catgctcaa 
      cgacgccga gaaacgcac agatgggag cagcaatat 
      gagcatcct gcccattgc ggccctcct gacggggtc 
      gctcctgac tctgggcat cgccgtgtt gctcatcgt 
      cgtgctggc cgtccgccg gaagcaccg gcactgcgg 
      cggtggcca ctgcagcta ccagctgga cccctcgaa 
      gcagcccaa agttccggg ccagtcctc caggcccgg 
      atcgatgct ggaaatcaa cacgggaga taatcgcta 
      cgtggtggc gtacaccct gaaaccggc agcggactg 
      cggcacgca gccctccgc caattcgtc cgtagatag 
      gcaaccaga tattctcaa cacccccag aggtgctga 
      cacgacaac gccacccgg gctgccgag acctgatgc 
      ccttttcac cccctcaac gggagaacg gaatcggca 
      gcagcacag ccccccgac attccccaa ctttagcca 
      cgaagagca gatctcgtc gccggaagt gtccgtgag 
      ccagacggg gggcacttt agggcggcc ccgtccgcg 
      ccctcgaga catttccta ttccaactt cgccacaat 
      gaggagaga ccacatcat agccgacgc cattccggg 
      acctgaaag ttgtgttta atgaactga gttgatcag 
      tgctaccac ctccagtta aaaactttc gtcattttt 
      cctaatcaa agtaatgat tcttccatt tgtaacaaa 
      cagcgggat taatctgga ggcggcagc actaacata 
      gcgcctgtg atacaggag tagatgcca agtgtttct 
      agcaaatga ttctgttta gatagtgga acaatcaca 
      gactctcga gcttaacta attgttttt agataggac 
      tttccgaaa actatcaac gaacaaact tgacaaatg 
      aatgaccga ccataattt tctaggtct atgaaatgt 
      acagtcgtg gtcgagaat tagttgatt tttaagagt 
      agtcagaga taattttcg gccgacgaa tgtaacgac 
      aaaacacta cttatacac gggcataat aaatgtgta 
      acttttaat acggatgaa tgttaatta aaatttaga 
      aaaaactaa tcatactgt gtatgattt atcgtaaac 
      agttttctt tctgatgtg agggaaaat gaggtaaca 
      aggagaaaa atataaata tctgatgaa acgttccca 
      cttcagtta cgccgttct ttcattaca tcatgcatt 
      aatgggctt ggaaaggcg ttcgaatta caaatacta 
      attaaaagc gttctctcc cagataagt cagtgtcaa 
      atttattaa atcgatagt cgaaaattt tcctacagt 
      aataaaagc ttggcgtat ctgtccaaa gactgtttc 
      cactatgta ataaattac aattaagtg tatatgtac 
      aataagtca gatactatg tacatatgt tattagatg 
      aatttgttt taacatata aaagcttta ttgctgtgc 
      aataatgta ttttgtaaa taaagtgtg cttactaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY