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Gene information
General information
TC033797
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008815,TC008816 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10339022..10367017
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033797
    iBeetle screen iB_08784

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033797 RA
      MRGRMEDPP KVELHLGST LNPEDIKEG DDVYFECII 
KANPKQHKI TWFHDGTLV SQNMSSGVI ISTHSLVLQ 
RVTRWQSGA YTCLAANPR GENVSNPVM LRVRYAPVC 
RDTEMAVIG ASLNEVIKV RCHVSADPS DVTFVWQFN 
NSGESFDVS PARISASIG NVSELMYTT SSQRDYGTL 
TCWGKNSIG RQAEPCVFQ VVPAAKPSP LTNCTLRAA 
TNHSSDVLE VECRAGYDG GLPQRFILE AYDAYTMRL 
RLNLSSAES DVPVFRLDL GEMLPPSGD GFPPSLRIV 
VYAQNAKGR SEKLVLEDI MLNDAEKRT DGSSNMSIL 
PIAALLTGS LLTLGIAVL LIVVLAVRR KHRHCGGGH 
CSYQLDPSK QPKVPGQSS RPGSMLEIN TGDNRYVVA 
YTLKPAADC GTQPSANSS VDRQPDILN TPRGADTTT 
PPGLPRPDA LFTPSTGER NRQQHSPPT FPNFSHEEQ 
ISSPEVSVS QTGGTLGRP RPRPRDISY SNFATMRRD 
HIIADAIPG PESCV
    CDS sequence
    • >TC033797 RA
      atgcgcggt cggatggag gatcccccc aaagtggag 
cttcacctg ggcagcacc ctcaacccc gaagacatc 
aaagaaggc gacgacgtg tacttcgag tgcatcatc 
aaagccaac cccaagcag cacaagatc acctggttc 
cacgacggc acgctggtg agtcagaac atgtcatcg 
ggcgtgatc atcagcaca cacagcctg gtcctgcag 
agggtcacg aggtggcag agcggcgcc tacacctgc 
ttggcggcc aacccccgc ggggagaac gtcagcaat 
cctgtcatg ctaagggtt agatatgct ccggtttgc 
cgcgacacc gagatggct gtaatcggg gcctccctc 
aacgaggtg atcaaagtg cgttgtcac gtgtcggcc 
gaccccagc gacgtaacc tttgtctgg cagttcaac 
aacagcggc gagagcttc gacgtgtct cctgcccgc 
atctcggcc tccatcggc aacgtgagc gagctcatg 
tacaccacc tcctcccag cgcgactac ggcaccctc 
acctgctgg ggcaagaac tcgatcggg cgccaggcg 
gagccctgc gtcttccaa gtggtcccc gcagccaag 
cccagcccc ctcaccaac tgcaccctg cgcgccgcc 
accaaccac agctcggac gtgctggag gtggagtgc 
cgcgccggc tacgacggc ggcctgccc cagcgcttc 
atcctggag gcgtacgac gcctacacc atgcgcctg 
cggctcaac ctcagcagc gccgagtcc gacgtcccc 
gtcttccgg ctggacctg ggggagatg ctgccgccc 
tccggggac ggcttcccg ccctcgctc cgcatcgtc 
gtctacgcg cagaacgcg aagggccgc agcgagaag 
ctcgtcctc gaggacatc atgctcaac gacgccgag 
aaacgcaca gatgggagc agcaatatg agcatcctg 
cccattgcg gccctcctg acggggtcg ctcctgact 
ctgggcatc gccgtgttg ctcatcgtc gtgctggcc 
gtccgccgg aagcaccgg cactgcggc ggtggccac 
tgcagctac cagctggac ccctcgaag cagcccaaa 
gttccgggc cagtcctcc aggcccgga tcgatgctg 
gaaatcaac acgggagat aatcgctac gtggtggcg 
tacaccctg aaaccggca gcggactgc ggcacgcag 
ccctccgcc aattcgtcc gtagatagg caaccagat 
attctcaac acccccaga ggtgctgac acgacaacg 
ccacccggg ctgccgaga cctgatgcc cttttcacc 
ccctcaacg ggagaacgg aatcggcag cagcacagc 
cccccgaca ttccccaac tttagccac gaagagcag 
atctcgtcg ccggaagtg tccgtgagc cagacgggg 
ggcacttta gggcggccc cgtccgcgc cctcgagac 
atttcctat tccaacttc gccacaatg aggagagac 
cacatcata gccgacgcc attccggga cctgaaagt 
tgtgtttaa
    mRNA sequence
    • >TC033797 RA
      gagtaattt tacgctcac attttcgaa agagaatat 
tttattacg tcttattta gcaacaagc aagcccgcg 
gttctttaa tatgcgcgg tcggatgga ggatccccc 
caaagtgga gcttcacct gggcagcac cctcaaccc 
cgaagacat caaagaagg cgacgacgt gtacttcga 
gtgcatcat caaagccaa ccccaagca gcacaagat 
cacctggtt ccacgacgg cacgctggt gagtcagaa 
catgtcatc gggcgtgat catcagcac acacagcct 
ggtcctgca gagggtcac gaggtggca gagcggcgc 
ctacacctg cttggcggc caacccccg cggggagaa 
cgtcagcaa tcctgtcat gctaagggt tagatatgc 
tccggtttg ccgcgacac cgagatggc tgtaatcgg 
ggcctccct caacgaggt gatcaaagt gcgttgtca 
cgtgtcggc cgaccccag cgacgtaac ctttgtctg 
gcagttcaa caacagcgg cgagagctt cgacgtgtc 
tcctgcccg catctcggc ctccatcgg caacgtgag 
cgagctcat gtacaccac ctcctccca gcgcgacta 
cggcaccct cacctgctg gggcaagaa ctcgatcgg 
gcgccaggc ggagccctg cgtcttcca agtggtccc 
cgcagccaa gcccagccc cctcaccaa ctgcaccct 
gcgcgccgc caccaacca cagctcgga cgtgctgga 
ggtggagtg ccgcgccgg ctacgacgg cggcctgcc 
ccagcgctt catcctgga ggcgtacga cgcctacac 
catgcgcct gcggctcaa cctcagcag cgccgagtc 
cgacgtccc cgtcttccg gctggacct gggggagat 
gctgccgcc ctccgggga cggcttccc gccctcgct 
ccgcatcgt cgtctacgc gcagaacgc gaagggccg 
cagcgagaa gctcgtcct cgaggacat catgctcaa 
cgacgccga gaaacgcac agatgggag cagcaatat 
gagcatcct gcccattgc ggccctcct gacggggtc 
gctcctgac tctgggcat cgccgtgtt gctcatcgt 
cgtgctggc cgtccgccg gaagcaccg gcactgcgg 
cggtggcca ctgcagcta ccagctgga cccctcgaa 
gcagcccaa agttccggg ccagtcctc caggcccgg 
atcgatgct ggaaatcaa cacgggaga taatcgcta 
cgtggtggc gtacaccct gaaaccggc agcggactg 
cggcacgca gccctccgc caattcgtc cgtagatag 
gcaaccaga tattctcaa cacccccag aggtgctga 
cacgacaac gccacccgg gctgccgag acctgatgc 
ccttttcac cccctcaac gggagaacg gaatcggca 
gcagcacag ccccccgac attccccaa ctttagcca 
cgaagagca gatctcgtc gccggaagt gtccgtgag 
ccagacggg gggcacttt agggcggcc ccgtccgcg 
ccctcgaga catttccta ttccaactt cgccacaat 
gaggagaga ccacatcat agccgacgc cattccggg 
acctgaaag ttgtgttta atgaactga gttgatcag 
tgctaccac ctccagtta aaaactttc gtcattttt 
cctaatcaa agtaatgat tcttccatt tgtaacaaa 
cagcgggat taatctgga ggcggcagc actaacata 
gcgcctgtg atacaggag tagatgcca agtgtttct 
agcaaatga ttctgttta gatagtgga acaatcaca 
gactctcga gcttaacta attgttttt agataggac 
tttccgaaa actatcaac gaacaaact tgacaaatg 
aatgaccga ccataattt tctaggtct atgaaatgt 
acagtcgtg gtcgagaat tagttgatt tttaagagt 
agtcagaga taattttcg gccgacgaa tgtaacgac 
aaaacacta cttatacac gggcataat aaatgtgta 
acttttaat acggatgaa tgttaatta aaatttaga 
aaaaactaa tcatactgt gtatgattt atcgtaaac 
agttttctt tctgatgtg agggaaaat gaggtaaca 
aggagaaaa atataaata tctgatgaa acgttccca 
cttcagtta cgccgttct ttcattaca tcatgcatt 
aatgggctt ggaaaggcg ttcgaatta caaatacta 
attaaaagc gttctctcc cagataagt cagtgtcaa 
atttattaa atcgatagt cgaaaattt tcctacagt 
aataaaagc ttggcgtat ctgtccaaa gactgtttc 
cactatgta ataaattac aattaagtg tatatgtac 
aataagtca gatactatg tacatatgt tattagatg 
aatttgttt taacatata aaagcttta ttgctgtgc 
aataatgta ttttgtaaa taaagtgtg cttactaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY