| General information | |||
| TC033797
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10339022..10367017 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033797 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08784 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033797 RA
MRGRMEDPP KVELHLGST LNPEDIKEG DDVYFECII KANPKQHKI TWFHDGTLV SQNMSSGVI ISTHSLVLQ RVTRWQSGA YTCLAANPR GENVSNPVM LRVRYAPVC RDTEMAVIG ASLNEVIKV RCHVSADPS DVTFVWQFN NSGESFDVS PARISASIG NVSELMYTT SSQRDYGTL TCWGKNSIG RQAEPCVFQ VVPAAKPSP LTNCTLRAA TNHSSDVLE VECRAGYDG GLPQRFILE AYDAYTMRL RLNLSSAES DVPVFRLDL GEMLPPSGD GFPPSLRIV VYAQNAKGR SEKLVLEDI MLNDAEKRT DGSSNMSIL PIAALLTGS LLTLGIAVL LIVVLAVRR KHRHCGGGH CSYQLDPSK QPKVPGQSS RPGSMLEIN TGDNRYVVA YTLKPAADC GTQPSANSS VDRQPDILN TPRGADTTT PPGLPRPDA LFTPSTGER NRQQHSPPT FPNFSHEEQ ISSPEVSVS QTGGTLGRP RPRPRDISY SNFATMRRD HIIADAIPG PESCV
- >TC033797 RA
atgcgcggt cggatggag gatcccccc aaagtggag cttcacctg ggcagcacc ctcaacccc gaagacatc aaagaaggc gacgacgtg tacttcgag tgcatcatc aaagccaac cccaagcag cacaagatc acctggttc cacgacggc acgctggtg agtcagaac atgtcatcg ggcgtgatc atcagcaca cacagcctg gtcctgcag agggtcacg aggtggcag agcggcgcc tacacctgc ttggcggcc aacccccgc ggggagaac gtcagcaat cctgtcatg ctaagggtt agatatgct ccggtttgc cgcgacacc gagatggct gtaatcggg gcctccctc aacgaggtg atcaaagtg cgttgtcac gtgtcggcc gaccccagc gacgtaacc tttgtctgg cagttcaac aacagcggc gagagcttc gacgtgtct cctgcccgc atctcggcc tccatcggc aacgtgagc gagctcatg tacaccacc tcctcccag cgcgactac ggcaccctc acctgctgg ggcaagaac tcgatcggg cgccaggcg gagccctgc gtcttccaa gtggtcccc gcagccaag cccagcccc ctcaccaac tgcaccctg cgcgccgcc accaaccac agctcggac gtgctggag gtggagtgc cgcgccggc tacgacggc ggcctgccc cagcgcttc atcctggag gcgtacgac gcctacacc atgcgcctg cggctcaac ctcagcagc gccgagtcc gacgtcccc gtcttccgg ctggacctg ggggagatg ctgccgccc tccggggac ggcttcccg ccctcgctc cgcatcgtc gtctacgcg cagaacgcg aagggccgc agcgagaag ctcgtcctc gaggacatc atgctcaac gacgccgag aaacgcaca gatgggagc agcaatatg agcatcctg cccattgcg gccctcctg acggggtcg ctcctgact ctgggcatc gccgtgttg ctcatcgtc gtgctggcc gtccgccgg aagcaccgg cactgcggc ggtggccac tgcagctac cagctggac ccctcgaag cagcccaaa gttccgggc cagtcctcc aggcccgga tcgatgctg gaaatcaac acgggagat aatcgctac gtggtggcg tacaccctg aaaccggca gcggactgc ggcacgcag ccctccgcc aattcgtcc gtagatagg caaccagat attctcaac acccccaga ggtgctgac acgacaacg ccacccggg ctgccgaga cctgatgcc cttttcacc ccctcaacg ggagaacgg aatcggcag cagcacagc cccccgaca ttccccaac tttagccac gaagagcag atctcgtcg ccggaagtg tccgtgagc cagacgggg ggcacttta gggcggccc cgtccgcgc cctcgagac atttcctat tccaacttc gccacaatg aggagagac cacatcata gccgacgcc attccggga cctgaaagt tgtgtttaa
- >TC033797 RA
gagtaattt tacgctcac attttcgaa agagaatat tttattacg tcttattta gcaacaagc aagcccgcg gttctttaa tatgcgcgg tcggatgga ggatccccc caaagtgga gcttcacct gggcagcac cctcaaccc cgaagacat caaagaagg cgacgacgt gtacttcga gtgcatcat caaagccaa ccccaagca gcacaagat cacctggtt ccacgacgg cacgctggt gagtcagaa catgtcatc gggcgtgat catcagcac acacagcct ggtcctgca gagggtcac gaggtggca gagcggcgc ctacacctg cttggcggc caacccccg cggggagaa cgtcagcaa tcctgtcat gctaagggt tagatatgc tccggtttg ccgcgacac cgagatggc tgtaatcgg ggcctccct caacgaggt gatcaaagt gcgttgtca cgtgtcggc cgaccccag cgacgtaac ctttgtctg gcagttcaa caacagcgg cgagagctt cgacgtgtc tcctgcccg catctcggc ctccatcgg caacgtgag cgagctcat gtacaccac ctcctccca gcgcgacta cggcaccct cacctgctg gggcaagaa ctcgatcgg gcgccaggc ggagccctg cgtcttcca agtggtccc cgcagccaa gcccagccc cctcaccaa ctgcaccct gcgcgccgc caccaacca cagctcgga cgtgctgga ggtggagtg ccgcgccgg ctacgacgg cggcctgcc ccagcgctt catcctgga ggcgtacga cgcctacac catgcgcct gcggctcaa cctcagcag cgccgagtc cgacgtccc cgtcttccg gctggacct gggggagat gctgccgcc ctccgggga cggcttccc gccctcgct ccgcatcgt cgtctacgc gcagaacgc gaagggccg cagcgagaa gctcgtcct cgaggacat catgctcaa cgacgccga gaaacgcac agatgggag cagcaatat gagcatcct gcccattgc ggccctcct gacggggtc gctcctgac tctgggcat cgccgtgtt gctcatcgt cgtgctggc cgtccgccg gaagcaccg gcactgcgg cggtggcca ctgcagcta ccagctgga cccctcgaa gcagcccaa agttccggg ccagtcctc caggcccgg atcgatgct ggaaatcaa cacgggaga taatcgcta cgtggtggc gtacaccct gaaaccggc agcggactg cggcacgca gccctccgc caattcgtc cgtagatag gcaaccaga tattctcaa cacccccag aggtgctga cacgacaac gccacccgg gctgccgag acctgatgc ccttttcac cccctcaac gggagaacg gaatcggca gcagcacag ccccccgac attccccaa ctttagcca cgaagagca gatctcgtc gccggaagt gtccgtgag ccagacggg gggcacttt agggcggcc ccgtccgcg ccctcgaga catttccta ttccaactt cgccacaat gaggagaga ccacatcat agccgacgc cattccggg acctgaaag ttgtgttta atgaactga gttgatcag tgctaccac ctccagtta aaaactttc gtcattttt cctaatcaa agtaatgat tcttccatt tgtaacaaa cagcgggat taatctgga ggcggcagc actaacata gcgcctgtg atacaggag tagatgcca agtgtttct agcaaatga ttctgttta gatagtgga acaatcaca gactctcga gcttaacta attgttttt agataggac tttccgaaa actatcaac gaacaaact tgacaaatg aatgaccga ccataattt tctaggtct atgaaatgt acagtcgtg gtcgagaat tagttgatt tttaagagt agtcagaga taattttcg gccgacgaa tgtaacgac aaaacacta cttatacac gggcataat aaatgtgta acttttaat acggatgaa tgttaatta aaatttaga aaaaactaa tcatactgt gtatgattt atcgtaaac agttttctt tctgatgtg agggaaaat gaggtaaca aggagaaaa atataaata tctgatgaa acgttccca cttcagtta cgccgttct ttcattaca tcatgcatt aatgggctt ggaaaggcg ttcgaatta caaatacta attaaaagc gttctctcc cagataagt cagtgtcaa atttattaa atcgatagt cgaaaattt tcctacagt aataaaagc ttggcgtat ctgtccaaa gactgtttc cactatgta ataaattac aattaagtg tatatgtac aataagtca gatactatg tacatatgt tattagatg aatttgttt taacatata aaagcttta ttgctgtgc aataatgta ttttgtaaa taaagtgtg cttactaca
