Status
Please wait ...
Gene information
General information
TC010001 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10498172..10514027
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010001
    iBeetle screen iB_08782

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010001 RA
      MRPKASLPI LLLLGVVFV ASPAEGHVA LTFPPARKY 
      DLDFLDSSR TKAPCGMPK GNIKTSFLS GGKFNVSWH 
      LGYPHRGGF KIQLLDHLE RPILDLTPT IQGSEFVSS 
      DATAQSFQV QLPKDFTCL NCTLRLIRQ ALEWGADYK 
      FWSCADVDI KTRKDFREV CSNHGRYLL GRCKCDRLY 
      YGTYCEFRD ECLEDSDCG SHGKCVDVQ ATTAPRMQC 
      YCQLGWFGP GCNKRSPVK NTEIDFESY THRKLSDTF 
      RLYWRILKE HKEIEVVMV VNGTSYVGL GWRPQSLTK 
      SCKNFPLIG VNESPATES KAEPEPEAK SEPEPEPTS 
      EPEPTSEPE PTSEPEPTS EPGAEPEPT TVKYKSKKS 
      QYSRRSAIP PLQKVHPNA DVVETSVSF QVSKKQGRS 
      RREVSEPVA EPEAEPEPT TAEPKSEPE PKSEPEPEP 
      KSEPEPEPK SEPEPEPKS EPEPKSEPE PKSEPEPKS 
      EPEPKSEPE PKSEPEPKS EPKPEPHLE SVKTHPKLQ 
      VAPTGKKET LNAYTPRHD FNPMDCTDI VIGSARGTA 
      SRIADYYTR DRSTPRKDN FWGGKNDLT AAMGFEKDG 
      ITTIVFRKK LQAIEPTDH SIEDKIMHV IFAQGQEPG 
      KYVHVPKSG VEAAKVSLK DFYKPDELK YHGHRSQRG 
      VSAINFFDE KKDAPVGGS PLPAEPGTN QTASQGGNL 
      SECGGEWRY PRTCDTLNN TCEYTAKWE VQKKDEIRF 
      TITTSHTDL WTGIAFSDD EKMSQTDAI LGWVDKTGR 
      PFLMDTWIS GYTQPFLDS SQGIYNTEG RIVDGVTTL 
      SFTRKRISN DPKDLSFTD DHCLFMMFP VKGGIFNSV 
      NKKIRKHEA VPVISLERI CIKSCGSDD DVVTTTEAP 
      GLSYNVAVK IVELGENFI VPKAGSLQY DDLSNSIND 
      NFKPIFRKI PGFRRAVIE DLKEEGNDL VATMNVQVD 
      KAMAEKGRS LSENSESND EQVHKMLQE SVGSGKIGN 
      LKVDPAYLV FEPQSLTSN IIQGSSSSE DNSFFNLSE 
      AKLYIVLGC IAALVLVAI VQASCTIYK AASRSSSSH 
      KDRLIPNSA WKDYSAANT NYAFDAFEA EEKKQQQMN 
      GKGRSDTLP PKSNNRIQR PQGRPPSVP PQNASNYMG 
      DTRSLQRPK GGYPGNLER ATFSLPRTT YDRQRPMPD 
      MQPDFYFMP SQRKYSGEV VRVYVDYNN QPK
    CDS sequence
    • >TC010001 RA
      atgagacca aaggcgtcc ctgccaatt ctccttctt 
      cttggcgtc gtcttcgtt gcgtctccg gcagaagga 
      cacgtggca cttaccttt ccaccggct cgaaaatat 
      gatctcgac ttcttagac agctctaga acgaaagca 
      ccatgtgga atgcctaaa ggcaacatt aaaacctcc 
      tttctgagc ggtggcaaa tttaatgtg tcgtggcac 
      ttaggatat ccccatcga gggggattc aaaatccag 
      ctcctggat cacctcgaa agaccaatt ttagactta 
      actcccaca attcaagga tcggaattt gtttcaagc 
      gatgcgacg gcccaatcc tttcaagta caactacca 
      aaagacttt acttgcctg aactgtacg ttgcgacta 
      atcagacag gcgctggaa tggggcgca gattacaaa 
      ttttggtca tgtgccgac gtcgatatc aaaacaaga 
      aaggacttt agggaagtg tgtagcaat cacggaagg 
      tacctttta gggaggtgc aaatgtgac aggctttat 
      tatggcacc tattgcgaa tttcgcgat gaatgttta 
      gaggacagc gactgcgga agtcacgga aaatgtgta 
      gacgttcaa gcaacaaca gcccctcga atgcagtgt 
      tactgccag ttaggctgg ttcgggccc ggatgtaac 
      aaacgttct cccgttaaa aacactgaa attgatttc 
      gaatcctac actcatcgc aaacttagc gacactttc 
      agactgtat tggaggatt ctgaaggag cacaaggag 
      atcgaagtt gttatggtc gtcaatgga accagttac 
      gtgggtctt ggctggcga ccgcaatcc ttaaccaaa 
      tcttgcaag aatttccct ttaattggc gtaaacgaa 
      tcaccggcc accgaaagt aaagccgaa ccggaaccg 
      gaagccaaa agcgaaccg gaacccgaa cctacaagt 
      gaaccggaa ccaacaagc gaacccgaa cccaccagt 
      gaacccgaa cccaccagc gaaccgggg gctgaacca 
      gaacccaca acagttaaa tacaaatcg aaaaaatcc 
      caatattcc agacgctct gctattcca cccctccag 
      aaagttcac ccgaatgcc gacgtcgtt gagacaagt 
      gtgtcgttc caggttagc aaaaaacaa ggacgaagc 
      cgacgagaa gtcagcgaa cctgtagca gaaccggaa 
      gcggaacca gaaccaacc accgctgaa cctaagtca 
      gaaccagaa cccaaatct gaacccgaa cccgaaccc 
      aagtcggaa cccgagccc gaacccaag tcggaacct 
      gaacctgaa cctaaatcg gaacccgaa cctaagtcc 
      gaacctgaa cccaaatcg gaacccgaa cccaagtct 
      gaacccgaa cccaaatcg gaacccgaa cccaagtct 
      gaacccgaa cccaaatcg gaacccaaa cctgaaccg 
      cacttagaa tcagttaaa actcacccc aaactacaa 
      gtagcaccc actggaaag aaagagact ttgaatgca 
      tacacccct cgtcatgac ttcaatccc atggactgt 
      accgacatt gtcatcggt tcagctcgt ggaacagct 
      agcagaatc gccgattat tacactcgt gataggtca 
      accccccga aaagacaac ttctggggc ggcaaaaac 
      gacctaact gctgccatg ggtttcgaa aaagacgga 
      attacaaca attgtcttc cgaaagaag ttgcaagca 
      atcgaacca acagaccat agcattgaa gacaaaata 
      atgcatgtc attttcgcc caaggacaa gaacccggc 
      aaatacgtc catgtgccc aaatctgga gtggaggca 
      gcaaaagtg tccctgaaa gatttctac aagcctgac 
      gaactcaaa tatcacgga cacaggtct cagagagga 
      gtttccgca attaatttc ttcgatgag aaaaaagac 
      gctcctgtt ggaggatca ccactcccg gctgagcca 
      ggaaccaat cagacggca agtcaaggg gggaacctc 
      tcggaatgt gggggagaa tggaggtac cctcgcacc 
      tgtgacact ctaaacaac acgtgcgag tacacagcc 
      aagtgggaa gtccagaag aaagacgag attcggttc 
      acgataacc acgagtcac actgacttg tggacggga 
      attgcgttt tcagatgat gaaaaaatg agccaaact 
      gacgcaatc ctgggatgg gtggacaaa acgggccgt 
      ccattcctg atggacacc tggataagt ggctacacc 
      caaccgttc ctggacagt tcccaaggg atttacaac 
      accgaagga cgcatcgtt gacggagtc acgacattg 
      tcgttcacc agaaaacgc atttcaaac gacccgaaa 
      gacttgtcg ttcactgat gatcactgc ctctttatg 
      atgttccct gtcaaggga ggaatcttc aacagtgtt 
      aataaaaag atcaggaag catgaagca gtcccggtc 
      atttcgttg gagaggatt tgtatcaag tcttgcgga 
      agtgacgac gatgttgta acaacaacg gaagcacct 
      ggcttgagc tacaacgtt gctgttaaa attgtggag 
      ttgggggag aatttcatc gtccccaaa gcgggctct 
      ttgcaatat gatgacttg tctaacagc atcaacgat 
      aattttaaa ccgatattt aggaagatt ccagggttt 
      aggagagcc gtgattgaa gatttgaaa gaggaaggt 
      aatgacttg gtggccaca atgaacgtc caagtcgac 
      aaagcaatg gccgaaaaa ggtcgttct ctatcggag 
      aactccgag tctaacgat gagcaagtt cacaaaatg 
      ctgcaagag agtgtaggg agcggaaaa attggtaat 
      ctcaaagta gatcccgct tatctagtt ttcgagcct 
      caaagcttg acatcaaac atcatccaa ggtagttct 
      tctagtgaa gacaacagt ttcttcaac ttatccgaa 
      gccaagttg tatatagtt ttaggctgc attgcagct 
      ttggttcta gtcgctata gttcaggct agttgcaca 
      atttataaa gccgccagc agaagttcg tcgagtcat 
      aaggaccgt ttgataccg aactctgca tggaaagac 
      tactctgcc gccaacaca aattacgca ttcgatgct 
      ttcgaagcc gaagagaag aaacaacag caaatgaac 
      ggcaaagga cgcagcgat acgctccct ccgaagagc 
      aacaaccgc atccagaga cctcaaggc cggccgccg 
      tccgtgccc ccgcagaac gcgagtaat tacatgggc 
      gatacgcgg tccttgcag cggcccaaa ggcggctat 
      cctgggaat ttagaaaga gcaacgttt tccctgccg 
      aggacgacg tacgacaga cagaggccg atgccggac 
      atgcagccc gatttttat ttcatgccg tcccagagg 
      aagtattcc ggagaagtg gtcagggtc tatgttgac 
      tacaacaat cagcccaag tga
    mRNA sequence
    • >TC010001 RA
      gcgtcgata tcaccttct tcaagtaaa atttctcga 
      tacaactag taccgactt agaaagtgt ttgaatcgt 
      acttgcgcg gaaacacag cgcgttgtg gtgacttca 
      acgagtggt ctagtggga ttttgttgt tactgcggc 
      aggtatcac cagttatgt ttcttccgt gttcggctt 
      cgtgaaaaa tgagaccaa aggcgtccc tgccaattc 
      tccttcttc ttggcgtcg tcttcgttg cgtctccgg 
      cagaaggac acgtggcac ttacctttc caccggctc 
      gaaaatatg atctcgact tcttagaca gctctagaa 
      cgaaagcac catgtggaa tgcctaaag gcaacatta 
      aaacctcct ttctgagcg gtggcaaat ttaatgtgt 
      cgtggcact taggatatc cccatcgag ggggattca 
      aaatccagc tcctggatc acctcgaaa gaccaattt 
      tagacttaa ctcccacaa ttcaaggat cggaatttg 
      tttcaagcg atgcgacgg cccaatcct ttcaagtac 
      aactaccaa aagacttta cttgcctga actgtacgt 
      tgcgactaa tcagacagg cgctggaat ggggcgcag 
      attacaaat tttggtcat gtgccgacg tcgatatca 
      aaacaagaa aggacttta gggaagtgt gtagcaatc 
      acggaaggt accttttag ggaggtgca aatgtgaca 
      ggctttatt atggcacct attgcgaat ttcgcgatg 
      aatgtttag aggacagcg actgcggaa gtcacggaa 
      aatgtgtag acgttcaag caacaacag cccctcgaa 
      tgcagtgtt actgccagt taggctggt tcgggcccg 
      gatgtaaca aacgttctc ccgttaaaa acactgaaa 
      ttgatttcg aatcctaca ctcatcgca aacttagcg 
      acactttca gactgtatt ggaggattc tgaaggagc 
      acaaggaga tcgaagttg ttatggtcg tcaatggaa 
      ccagttacg tgggtcttg gctggcgac cgcaatcct 
      taaccaaat cttgcaaga atttccctt taattggcg 
      taaacgaat caccggcca ccgaaagta aagccgaac 
      cggaaccgg aagccaaaa gcgaaccgg aacccgaac 
      ctacaagtg aaccggaac caacaagcg aacccgaac 
      ccaccagtg aacccgaac ccaccagcg aaccggggg 
      ctgaaccag aacccacaa cagttaaat acaaatcga 
      aaaaatccc aatattcca gacgctctg ctattccac 
      ccctccaga aagttcacc cgaatgccg acgtcgttg 
      agacaagtg tgtcgttcc aggttagca aaaaacaag 
      gacgaagcc gacgagaag tcagcgaac ctgtagcag 
      aaccggaag cggaaccag aaccaacca ccgctgaac 
      ctaagtcag aaccagaac ccaaatctg aacccgaac 
      ccgaaccca agtcggaac ccgagcccg aacccaagt 
      cggaacctg aacctgaac ctaaatcgg aacccgaac 
      ctaagtccg aacctgaac ccaaatcgg aacccgaac 
      ccaagtctg aacccgaac ccaaatcgg aacccgaac 
      ccaagtctg aacccgaac ccaaatcgg aacccaaac 
      ctgaaccgc acttagaat cagttaaaa ctcacccca 
      aactacaag tagcaccca ctggaaaga aagagactt 
      tgaatgcat acacccctc gtcatgact tcaatccca 
      tggactgta ccgacattg tcatcggtt cagctcgtg 
      gaacagcta gcagaatcg ccgattatt acactcgtg 
      ataggtcaa ccccccgaa aagacaact tctggggcg 
      gcaaaaacg acctaactg ctgccatgg gtttcgaaa 
      aagacggaa ttacaacaa ttgtcttcc gaaagaagt 
      tgcaagcaa tcgaaccaa cagaccata gcattgaag 
      acaaaataa tgcatgtca ttttcgccc aaggacaag 
      aacccggca aatacgtcc atgtgccca aatctggag 
      tggaggcag caaaagtgt ccctgaaag atttctaca 
      agcctgacg aactcaaat atcacggac acaggtctc 
      agagaggag tttccgcaa ttaatttct tcgatgaga 
      aaaaagacg ctcctgttg gaggatcac cactcccgg 
      ctgagccag gaaccaatc agacggcaa gtcaagggg 
      ggaacctct cggaatgtg ggggagaat ggaggtacc 
      ctcgcacct gtgacactc taaacaaca cgtgcgagt 
      acacagcca agtgggaag tccagaaga aagacgaga 
      ttcggttca cgataacca cgagtcaca ctgacttgt 
      ggacgggaa ttgcgtttt cagatgatg aaaaaatga 
      gccaaactg acgcaatcc tgggatggg tggacaaaa 
      cgggccgtc cattcctga tggacacct ggataagtg 
      gctacaccc aaccgttcc tggacagtt cccaaggga 
      tttacaaca ccgaaggac gcatcgttg acggagtca 
      cgacattgt cgttcacca gaaaacgca tttcaaacg 
      acccgaaag acttgtcgt tcactgatg atcactgcc 
      tctttatga tgttccctg tcaagggag gaatcttca 
      acagtgtta ataaaaaga tcaggaagc atgaagcag 
      tcccggtca tttcgttgg agaggattt gtatcaagt 
      cttgcggaa gtgacgacg atgttgtaa caacaacgg 
      aagcacctg gcttgagct acaacgttg ctgttaaaa 
      ttgtggagt tgggggaga atttcatcg tccccaaag 
      cgggctctt tgcaatatg atgacttgt ctaacagca 
      tcaacgata attttaaac cgatattta ggaagattc 
      cagggttta ggagagccg tgattgaag atttgaaag 
      aggaaggta atgacttgg tggccacaa tgaacgtcc 
      aagtcgaca aagcaatgg ccgaaaaag gtcgttctc 
      tatcggaga actccgagt ctaacgatg agcaagttc 
      acaaaatgc tgcaagaga gtgtaggga gcggaaaaa 
      ttggtaatc tcaaagtag atcccgctt atctagttt 
      tcgagcctc aaagcttga catcaaaca tcatccaag 
      gtagttctt ctagtgaag acaacagtt tcttcaact 
      tatccgaag ccaagttgt atatagttt taggctgca 
      ttgcagctt tggttctag tcgctatag ttcaggcta 
      gttgcacaa tttataaag ccgccagca gaagttcgt 
      cgagtcata aggaccgtt tgataccga actctgcat 
      ggaaagact actctgccg ccaacacaa attacgcat 
      tcgatgctt tcgaagccg aagagaaga aacaacagc 
      aaatgaacg gcaaaggac gcagcgata cgctccctc 
      cgaagagca acaaccgca tccagagac ctcaaggcc 
      ggccgccgt ccgtgcccc cgcagaacg cgagtaatt 
      acatgggcg atacgcggt ccttgcagc ggcccaaag 
      gcggctatc ctgggaatt tagaaagag caacgtttt 
      ccctgccga ggacgacgt acgacagac agaggccga 
      tgccggaca tgcagcccg atttttatt tcatgccgt 
      cccagagga agtattccg gagaagtgg tcagggtct 
      atgttgact acaacaatc agcccaagt gattgcgag 
      ctggggcac gtcatgcga cgtggagcg atttatcag 
      taccttgag tcttagatt ttaacggtt ttaattatt 
      gaattttaa gttgattgc gtaatttat ttttgtaca 
      tatggcgta tttattgta ttataactt attcaaagt 
      cggagccat ttgtacgaa aaataagcg tggggtaac 
      ttttctgtg cggaacgta tccacttaa atactcaac 
      caaagttag gtacagcta cagtaaggt ttaggctat 
      tgtatattg taaatttcc agggcaccc cataagtgt 
      tttagctgt ttttagcaa gtttagcgc tgcaggact 
      ggatactca aacacagag cttcggctt ttattttgt 
      gtcgcttat tgtaaatat ttacaatac taaatagat 
      taaaaaaca cgtgaattc gccctaagt catttttag 
      ttttaaatg ggtaatttt tttattatt gagcggcgt 
      gacacaaat taaaagtag aataataaa ttacaatct 
      tagaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY