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Gene information
General information
TC008832 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10564014..10575460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008832
    iBeetle screen iB_08779

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008832 RA
      MYRVGDYVY FETSSTSPY QIRRIEELN KTASGNVEA 
      KVMCFYRRR DLPSQLIQL ADKHQCALD ESNGSPVSD 
      SGSRGCESL NPKQRHQMK HRELFLSRQ VETLPATHI 
      RGKCSVTLF NETESLSSY LGKDDMFFY CLVFDPTQK 
      TLLADKGEI RVGSRYQCD VTPLLKEGE SDERDLSEL 
      ETLIWTPDH NLTDRQIDQ FLVVSRSVG TFARALDCS 
      SSVKQPSLH MSAAAASRD ITLFHAMDT LHHHNYDLA 
      SALCSLVPS SGPVLCRDE MEEWSASEA NLFEEALDK 
      YGKDFNDIR QDFLPWKTL KNIIEYYYM WKTTDRYVQ 
      QKRVKAVEA ESKLKQVYI PNYNKPNPA ALNNNKGVV 
      NGNSNGNSD TAVSLGGKS CESCNVTVS TQWYAWGPS 
      HMQCRLCHQ CWQYWKKYG GLKIPTRFG DGDFDNGGK 
      KKSGSDVDD DRTSAMPHR PHRCNIGGC GKEFKLKAH 
      LGRHYATSH GIVVRSGSP RPIMKTRTA FYLFTTPLT 
      RISRRLCRH IMKPRHAAR APFFAINIQ AVKQECAAQ 
      MIGKSLADL KQLLVYRKK NRGSVTAIA TRLGNPGSI 
      TPQWLILTD KDSLPKPEK VAFPKPPKA PDGSLVYER 
      VPNKPEADK IPLNNMSPS LKRRAYDEM NGIDSSALS 
      APPPKRLSK DPISMVRPD QYSMISSSI VPGQVLPRH 
      ISQINGKPK IAHMTRAGS GRKQVISWM DAPDDVYFR 
      ATEGSKRMR RQVTLAELR RAARKPWRQ LKTKMEEHP 
      IVVLD
    CDS sequence
    • >TC008832 RA
      atgtatcgt gtcggagat tacgtgtac tttgagacg 
      tcatcgacg tccccgtac cagatccgg cgaatcgag 
      gagctgaat aagacggca tcgggcaat gtggaagcg 
      aaggtgatg tgcttctac cgccggagg gacctgccc 
      agccagctg atccagctc gccgacaaa caccaatgt 
      gccttagac gagtcaaac ggcagcccg gtgtcggat 
      tcgggcagc agaggttgc gaatcctta aatccaaaa 
      caaaggcac caaatgaaa caccgcgag ttgttccta 
      tcccgtcag gtggaaacg ctaccggcc actcatatc 
      cgaggcaag tgttctgtg acgttgttt aatgagaca 
      gaatcgctc agttcctat ctgggcaaa gatgacatg 
      tttttctac tgtctggtg ttcgatccg acccaaaag 
      acgttgttg gccgacaag ggtgaaatt cgcgtaggt 
      agccggtac cagtgcgac gtaacacct ctactaaaa 
      gagggcgaa agtgacgaa cgcgacctc tccgaactc 
      gaaacccta atctggact ccggaccac aacctcacc 
      gaccgacaa atagatcag tttttagtc gtttcgcga 
      tcggtgggg acgtttgcc cgcgccttg gactgcagt 
      tcgagtgtc aaacagccc tcgttgcac atgtcggcg 
      gcggccgcc agccgggac attacgttg tttcacgcg 
      atggacact ctgcatcat cacaactac gatctggcg 
      agtgctctg tgttcgtta gttccttcg agcgggccg 
      gtgctgtgc cgggacgag atggaggag tggagtgcg 
      tcggaggct aatctgttt gaagaggcg ctcgacaaa 
      tacggcaag gatttcaac gatatacgt caggatttt 
      ctaccgtgg aagacgttg aaaaacatc atcgaatac 
      tactacatg tggaagact acggaccgc tacgtacaa 
      cagaaacgt gtcaaagcc gtcgaagct gaatccaaa 
      ttgaagcaa gtctacatt cccaactac aataaacca 
      aatccggcc gcacttaat aataataaa ggtgtcgtc 
      aatggtaac agtaacggc aattcagat acagctgtc 
      tcgttgggg ggtaaatcg tgcgagtct tgtaatgtg 
      acggtatcc actcaatgg tacgcttgg ggcccctcg 
      cacatgcag tgtcgtctt tgccaccag tgttggcaa 
      tattggaaa aaatacggc ggcttgaaa atcccgacc 
      cgtttcggc gacggcgat ttcgataac ggcggcaag 
      aaaaaatcg ggtagcgat gtcgacgac gaccgcacg 
      tcagcaatg ccacaccga ccgcatcgt tgcaacatc 
      ggcggttgc ggcaaagag ttcaaactt aaagcccat 
      ctggggcga cattatgcc acatcgcac ggcatagtg 
      gtgcgttca gggtcgcca cgacctata atgaaaacc 
      cgtacggcg ttctatttg tttacgacg ccgttgaca 
      aggatctcc aggaggttg tgcaggcac ataatgaaa 
      cctcggcac gcggcacgt gccccattc ttcgcgatc 
      aacatccaa gccgtcaaa caggagtgc gccgctcaa 
      atgataggc aaatcgctc gccgatctc aaacaattg 
      ctggtctat cgaaagaag aatcgagga agcgtcaca 
      gcgatcgca acgagattg gggaatccc ggctctatc 
      acgccgcaa tggctcata ttgactgat aaagatagt 
      ttgccaaaa ccggaaaaa gtggcgttt ccgaagccc 
      cccaaagcg cccgacggc agtttagtc tacgaaagg 
      gtgcccaat aaaccggag gccgacaaa ataccactc 
      aataacatg tcaccgtcg ctaaagagg cgtgcgtat 
      gatgaaatg aacggaatt gacagttcc gcgttgagt 
      gcgccgccc ccgaagcga ttgagcaaa gatccgatt 
      tcgatggtg cgtcccgat cagtattcg atgatatcg 
      tcgtcaata gttcccggg caagtgttg cctcgacat 
      atatctcag ataaacggt aaaccaaaa atcgcacat 
      atgactagg gctggttcc ggtcgaaag caggtcata 
      agctggatg gatgcccct gatgatgtg tattttcga 
      gcgacggaa gggagcaag aggatgaga cgacaagta 
      acgctagca gaattgaga agagcagct cgtaagccg 
      tggagacag ttgaaaacc aaaatggag gagcatcca 
      attgtggta cttgattga
    mRNA sequence
    • >TC008832 RA
      agacgccaa cagggagtt tgatttgag aaaatggcg 
      tctgttggc ttgatggtg tttagtgta ctgacaaaa 
      cattgaaga aattcgacg aaattattt aaaatgtgt 
      gtcgtatta acgtagtat tactgttag gagtaaatc 
      gtttttgtg tttgaagat gacagctaa catgtatcg 
      tgtcggaga ttacgtgta ctttgagac gtcatcgac 
      gtccccgta ccagatccg gcgaatcga ggagctgaa 
      taagacggc atcgggcaa tgtggaagc gaaggtgat 
      gtgcttcta ccgccggag ggacctgcc cagccagct 
      gatccagct cgccgacaa acaccaatg tgccttaga 
      cgagtcaaa cggcagccc ggtgtcgga ttcgggcag 
      cagaggttg cgaatcctt aaatccaaa acaaaggca 
      ccaaatgaa acaccgcga gttgttcct atcccgtca 
      ggtggaaac gctaccggc cactcatat ccgaggcaa 
      gtgttctgt gacgttgtt taatgagac agaatcgct 
      cagttccta tctgggcaa agatgacat gtttttcta 
      ctgtctggt gttcgatcc gacccaaaa gacgttgtt 
      ggccgacaa gggtgaaat tcgcgtagg tagccggta 
      ccagtgcga cgtaacacc tctactaaa agagggcga 
      aagtgacga acgcgacct ctccgaact cgaaaccct 
      aatctggac tccggacca caacctcac cgaccgaca 
      aatagatca gtttttagt cgtttcgcg atcggtggg 
      gacgtttgc ccgcgcctt ggactgcag ttcgagtgt 
      caaacagcc ctcgttgca catgtcggc ggcggccgc 
      cagccggga cattacgtt gtttcacgc gatggacac 
      tctgcatca tcacaacta cgatctggc gagtgctct 
      gtgttcgtt agttccttc gagcgggcc ggtgctgtg 
      ccgggacga gatggagga gtggagtgc gtcggaggc 
      taatctgtt tgaagaggc gctcgacaa atacggcaa 
      ggatttcaa cgatatacg tcaggattt tctaccgtg 
      gaagacgtt gaaaaacat catcgaata ctactacat 
      gtggaagac tacggaccg ctacgtaca acagaaacg 
      tgtcaaagc cgtcgaagc tgaatccaa attgaagca 
      agtctacat tcccaacta caataaacc aaatccggc 
      cgcacttaa taataataa aggtgtcgt caatggtaa 
      cagtaacgg caattcaga tacagctgt ctcgttggg 
      gggtaaatc gtgcgagtc ttgtaatgt gacggtatc 
      cactcaatg gtacgcttg gggcccctc gcacatgca 
      gtgtcgtct ttgccacca gtgttggca atattggaa 
      aaaatacgg cggcttgaa aatcccgac ccgtttcgg 
      cgacggcga tttcgataa cggcggcaa gaaaaaatc 
      gggtagcga tgtcgacga cgaccgcac gtcagcaat 
      gccacaccg accgcatcg ttgcaacat cggcggttg 
      cggcaaaga gttcaaact taaagccca tctggggcg 
      acattatgc cacatcgca cggcatagt ggtgcgttc 
      agggtcgcc acgacctat aatgaaaac ccgtacggc 
      gttctattt gtttacgac gccgttgac aaggatctc 
      caggaggtt gtgcaggca cataatgaa acctcggca 
      cgcggcacg tgccccatt cttcgcgat caacatcca 
      agccgtcaa acaggagtg cgccgctca aatgatagg 
      caaatcgct cgccgatct caaacaatt gctggtcta 
      tcgaaagaa gaatcgagg aagcgtcac agcgatcgc 
      aacgagatt ggggaatcc cggctctat cacgccgca 
      atggctcat attgactga taaagatag tttgccaaa 
      accggaaaa agtggcgtt tccgaagcc ccccaaagc 
      gcccgacgg cagtttagt ctacgaaag ggtgcccaa 
      taaaccgga ggccgacaa aataccact caataacat 
      gtcaccgtc gctaaagag gcgtgcgta tgatgaaat 
      gaacggaat tgacagttc cgcgttgag tgcgccgcc 
      cccgaagcg attgagcaa agatccgat ttcgatggt 
      gcgtcccga tcagtattc gatgatatc gtcgtcaat 
      agttcccgg gcaagtgtt gcctcgaca tatatctca 
      gataaacgg taaaccaaa aatcgcaca tatgactag 
      ggctggttc cggtcgaaa gcaggtcat aagctggat 
      ggatgcccc tgatgatgt gtattttcg agcgacgga 
      agggagcaa gaggatgag acgacaagt aacgctagc 
      agaattgag aagagcagc tcgtaagcc gtggagaca 
      gttgaaaac caaaatgga ggagcatcc aattgtggt 
      acttgattg acaatagat ttgtgtata gacgttcgt 
      gttgtcatc atcaagtgc tcagtgtac acgaaactg 
      gtggaacac ctctagcaa ccttttatt aaacgaatt 
      gtttttaaa cttggtatt tgtgttttt gaaatcatg 
      aagtgtctt ttataataa ttttacaaa tacgttatg 
      ttgtgtata taatattta tacagtttg ttgtatcag 
      gtatttatt ctaataggc aatgctagg tggtgaaga 
      actgtatat tgtacaatt tgtttactg gcaataatc 
      caagtgttg gaaaaacgt tgcgggact agtcttatt 
      gacagtgaa aattatgaa cattgacac aactatgta 
      gataccatt ctattaaat tgtgctact tggatagga 
      tcatatttc aagttgtaa aacgtagaa taatttaca 
      tttttttca taaataaac aagcactta gaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY