Status
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Gene information
General information
TC008832 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10564014..10575460
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC008832
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08779

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008832 RA
      MYRVGDYVY FETSSTSPY QIRRIEELN KTASGNVEA 
KVMCFYRRR DLPSQLIQL ADKHQCALD ESNGSPVSD 
SGSRGCESL NPKQRHQMK HRELFLSRQ VETLPATHI 
RGKCSVTLF NETESLSSY LGKDDMFFY CLVFDPTQK 
TLLADKGEI RVGSRYQCD VTPLLKEGE SDERDLSEL 
ETLIWTPDH NLTDRQIDQ FLVVSRSVG TFARALDCS 
SSVKQPSLH MSAAAASRD ITLFHAMDT LHHHNYDLA 
SALCSLVPS SGPVLCRDE MEEWSASEA NLFEEALDK 
YGKDFNDIR QDFLPWKTL KNIIEYYYM WKTTDRYVQ 
QKRVKAVEA ESKLKQVYI PNYNKPNPA ALNNNKGVV 
NGNSNGNSD TAVSLGGKS CESCNVTVS TQWYAWGPS 
HMQCRLCHQ CWQYWKKYG GLKIPTRFG DGDFDNGGK 
KKSGSDVDD DRTSAMPHR PHRCNIGGC GKEFKLKAH 
LGRHYATSH GIVVRSGSP RPIMKTRTA FYLFTTPLT 
RISRRLCRH IMKPRHAAR APFFAINIQ AVKQECAAQ 
MIGKSLADL KQLLVYRKK NRGSVTAIA TRLGNPGSI 
TPQWLILTD KDSLPKPEK VAFPKPPKA PDGSLVYER 
VPNKPEADK IPLNNMSPS LKRRAYDEM NGIDSSALS 
APPPKRLSK DPISMVRPD QYSMISSSI VPGQVLPRH 
ISQINGKPK IAHMTRAGS GRKQVISWM DAPDDVYFR 
ATEGSKRMR RQVTLAELR RAARKPWRQ LKTKMEEHP 
IVVLD
    CDS sequence
    • >TC008832 RA
      atgtatcgt gtcggagat tacgtgtac tttgagacg 
tcatcgacg tccccgtac cagatccgg cgaatcgag 
gagctgaat aagacggca tcgggcaat gtggaagcg 
aaggtgatg tgcttctac cgccggagg gacctgccc 
agccagctg atccagctc gccgacaaa caccaatgt 
gccttagac gagtcaaac ggcagcccg gtgtcggat 
tcgggcagc agaggttgc gaatcctta aatccaaaa 
caaaggcac caaatgaaa caccgcgag ttgttccta 
tcccgtcag gtggaaacg ctaccggcc actcatatc 
cgaggcaag tgttctgtg acgttgttt aatgagaca 
gaatcgctc agttcctat ctgggcaaa gatgacatg 
tttttctac tgtctggtg ttcgatccg acccaaaag 
acgttgttg gccgacaag ggtgaaatt cgcgtaggt 
agccggtac cagtgcgac gtaacacct ctactaaaa 
gagggcgaa agtgacgaa cgcgacctc tccgaactc 
gaaacccta atctggact ccggaccac aacctcacc 
gaccgacaa atagatcag tttttagtc gtttcgcga 
tcggtgggg acgtttgcc cgcgccttg gactgcagt 
tcgagtgtc aaacagccc tcgttgcac atgtcggcg 
gcggccgcc agccgggac attacgttg tttcacgcg 
atggacact ctgcatcat cacaactac gatctggcg 
agtgctctg tgttcgtta gttccttcg agcgggccg 
gtgctgtgc cgggacgag atggaggag tggagtgcg 
tcggaggct aatctgttt gaagaggcg ctcgacaaa 
tacggcaag gatttcaac gatatacgt caggatttt 
ctaccgtgg aagacgttg aaaaacatc atcgaatac 
tactacatg tggaagact acggaccgc tacgtacaa 
cagaaacgt gtcaaagcc gtcgaagct gaatccaaa 
ttgaagcaa gtctacatt cccaactac aataaacca 
aatccggcc gcacttaat aataataaa ggtgtcgtc 
aatggtaac agtaacggc aattcagat acagctgtc 
tcgttgggg ggtaaatcg tgcgagtct tgtaatgtg 
acggtatcc actcaatgg tacgcttgg ggcccctcg 
cacatgcag tgtcgtctt tgccaccag tgttggcaa 
tattggaaa aaatacggc ggcttgaaa atcccgacc 
cgtttcggc gacggcgat ttcgataac ggcggcaag 
aaaaaatcg ggtagcgat gtcgacgac gaccgcacg 
tcagcaatg ccacaccga ccgcatcgt tgcaacatc 
ggcggttgc ggcaaagag ttcaaactt aaagcccat 
ctggggcga cattatgcc acatcgcac ggcatagtg 
gtgcgttca gggtcgcca cgacctata atgaaaacc 
cgtacggcg ttctatttg tttacgacg ccgttgaca 
aggatctcc aggaggttg tgcaggcac ataatgaaa 
cctcggcac gcggcacgt gccccattc ttcgcgatc 
aacatccaa gccgtcaaa caggagtgc gccgctcaa 
atgataggc aaatcgctc gccgatctc aaacaattg 
ctggtctat cgaaagaag aatcgagga agcgtcaca 
gcgatcgca acgagattg gggaatccc ggctctatc 
acgccgcaa tggctcata ttgactgat aaagatagt 
ttgccaaaa ccggaaaaa gtggcgttt ccgaagccc 
cccaaagcg cccgacggc agtttagtc tacgaaagg 
gtgcccaat aaaccggag gccgacaaa ataccactc 
aataacatg tcaccgtcg ctaaagagg cgtgcgtat 
gatgaaatg aacggaatt gacagttcc gcgttgagt 
gcgccgccc ccgaagcga ttgagcaaa gatccgatt 
tcgatggtg cgtcccgat cagtattcg atgatatcg 
tcgtcaata gttcccggg caagtgttg cctcgacat 
atatctcag ataaacggt aaaccaaaa atcgcacat 
atgactagg gctggttcc ggtcgaaag caggtcata 
agctggatg gatgcccct gatgatgtg tattttcga 
gcgacggaa gggagcaag aggatgaga cgacaagta 
acgctagca gaattgaga agagcagct cgtaagccg 
tggagacag ttgaaaacc aaaatggag gagcatcca 
attgtggta cttgattga
    mRNA sequence
    • >TC008832 RA
      agacgccaa cagggagtt tgatttgag aaaatggcg 
tctgttggc ttgatggtg tttagtgta ctgacaaaa 
cattgaaga aattcgacg aaattattt aaaatgtgt 
gtcgtatta acgtagtat tactgttag gagtaaatc 
gtttttgtg tttgaagat gacagctaa catgtatcg 
tgtcggaga ttacgtgta ctttgagac gtcatcgac 
gtccccgta ccagatccg gcgaatcga ggagctgaa 
taagacggc atcgggcaa tgtggaagc gaaggtgat 
gtgcttcta ccgccggag ggacctgcc cagccagct 
gatccagct cgccgacaa acaccaatg tgccttaga 
cgagtcaaa cggcagccc ggtgtcgga ttcgggcag 
cagaggttg cgaatcctt aaatccaaa acaaaggca 
ccaaatgaa acaccgcga gttgttcct atcccgtca 
ggtggaaac gctaccggc cactcatat ccgaggcaa 
gtgttctgt gacgttgtt taatgagac agaatcgct 
cagttccta tctgggcaa agatgacat gtttttcta 
ctgtctggt gttcgatcc gacccaaaa gacgttgtt 
ggccgacaa gggtgaaat tcgcgtagg tagccggta 
ccagtgcga cgtaacacc tctactaaa agagggcga 
aagtgacga acgcgacct ctccgaact cgaaaccct 
aatctggac tccggacca caacctcac cgaccgaca 
aatagatca gtttttagt cgtttcgcg atcggtggg 
gacgtttgc ccgcgcctt ggactgcag ttcgagtgt 
caaacagcc ctcgttgca catgtcggc ggcggccgc 
cagccggga cattacgtt gtttcacgc gatggacac 
tctgcatca tcacaacta cgatctggc gagtgctct 
gtgttcgtt agttccttc gagcgggcc ggtgctgtg 
ccgggacga gatggagga gtggagtgc gtcggaggc 
taatctgtt tgaagaggc gctcgacaa atacggcaa 
ggatttcaa cgatatacg tcaggattt tctaccgtg 
gaagacgtt gaaaaacat catcgaata ctactacat 
gtggaagac tacggaccg ctacgtaca acagaaacg 
tgtcaaagc cgtcgaagc tgaatccaa attgaagca 
agtctacat tcccaacta caataaacc aaatccggc 
cgcacttaa taataataa aggtgtcgt caatggtaa 
cagtaacgg caattcaga tacagctgt ctcgttggg 
gggtaaatc gtgcgagtc ttgtaatgt gacggtatc 
cactcaatg gtacgcttg gggcccctc gcacatgca 
gtgtcgtct ttgccacca gtgttggca atattggaa 
aaaatacgg cggcttgaa aatcccgac ccgtttcgg 
cgacggcga tttcgataa cggcggcaa gaaaaaatc 
gggtagcga tgtcgacga cgaccgcac gtcagcaat 
gccacaccg accgcatcg ttgcaacat cggcggttg 
cggcaaaga gttcaaact taaagccca tctggggcg 
acattatgc cacatcgca cggcatagt ggtgcgttc 
agggtcgcc acgacctat aatgaaaac ccgtacggc 
gttctattt gtttacgac gccgttgac aaggatctc 
caggaggtt gtgcaggca cataatgaa acctcggca 
cgcggcacg tgccccatt cttcgcgat caacatcca 
agccgtcaa acaggagtg cgccgctca aatgatagg 
caaatcgct cgccgatct caaacaatt gctggtcta 
tcgaaagaa gaatcgagg aagcgtcac agcgatcgc 
aacgagatt ggggaatcc cggctctat cacgccgca 
atggctcat attgactga taaagatag tttgccaaa 
accggaaaa agtggcgtt tccgaagcc ccccaaagc 
gcccgacgg cagtttagt ctacgaaag ggtgcccaa 
taaaccgga ggccgacaa aataccact caataacat 
gtcaccgtc gctaaagag gcgtgcgta tgatgaaat 
gaacggaat tgacagttc cgcgttgag tgcgccgcc 
cccgaagcg attgagcaa agatccgat ttcgatggt 
gcgtcccga tcagtattc gatgatatc gtcgtcaat 
agttcccgg gcaagtgtt gcctcgaca tatatctca 
gataaacgg taaaccaaa aatcgcaca tatgactag 
ggctggttc cggtcgaaa gcaggtcat aagctggat 
ggatgcccc tgatgatgt gtattttcg agcgacgga 
agggagcaa gaggatgag acgacaagt aacgctagc 
agaattgag aagagcagc tcgtaagcc gtggagaca 
gttgaaaac caaaatgga ggagcatcc aattgtggt 
acttgattg acaatagat ttgtgtata gacgttcgt 
gttgtcatc atcaagtgc tcagtgtac acgaaactg 
gtggaacac ctctagcaa ccttttatt aaacgaatt 
gtttttaaa cttggtatt tgtgttttt gaaatcatg 
aagtgtctt ttataataa ttttacaaa tacgttatg 
ttgtgtata taatattta tacagtttg ttgtatcag 
gtatttatt ctaataggc aatgctagg tggtgaaga 
actgtatat tgtacaatt tgtttactg gcaataatc 
caagtgttg gaaaaacgt tgcgggact agtcttatt 
gacagtgaa aattatgaa cattgacac aactatgta 
gataccatt ctattaaat tgtgctact tggatagga 
tcatatttc aagttgtaa aacgtagaa taatttaca 
tttttttca taaataaac aagcactta gaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY