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Gene information
General information
TC033776
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009973
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10957271..10960328
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033776
    iBeetle screen iB_08772

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033776 RA
      MILSRLKYY GKNTTLHGL KYVADEKRR PVIRLTWIA 
      IFILSFYGM ILVFKASYK SYTENAISF VTETAYLNW 
      NTSFPAVTL CEITSSEVA WNGDKEEAE AVQQFIADL 
      IFFTGSCYS CHTPCSICE ELDFGDIVE KFRKKCSQL 
      LSKCKWNET PFDCCEKFL PLETEYGIC YSFNSLHTK 
      KPSQHASDF YFFSNRKTG PGALFIQTI EDVRLYFHA 
      REDVPFINS DPDQRKDVM LGETYNISI NIIEIENDE 
      NVKLLPVEK RKCRFPEEK PGDLIVHDY YSYSTCVVQ 
      CHADAHIDL CNCTHHLMP VLNKEKYCD IEGLKCLTD 
      NFETVNRLH AKGFDKPGL VCDCLPSCI EPEYKVVAD 
      TKGVKSSHG LRWT
    CDS sequence
    • >TC033776 RA
      atgatcctg tccagatta aaatattac ggaaaaaac 
      acaactctg cacgggcta aaatacgta gccgacgaa 
      aagagacgt cccgtgata agattgacc tggatagca 
      atttttatc ttgagcttt tatggtatg atattagtc 
      ttcaaagct tcgtataaa tcatacacc gagaacgcc 
      atcagtttc gtcacagaa accgcttac ttaaactgg 
      aacacttct tttccagct gtcacactt tgcgaaatt 
      accagttcg gaagtggcc tggaacggg gataaggaa 
      gaggccgag gccgtccag cagtttatt gcagatttg 
      atatttttt accggcagc tgctattct tgtcacaca 
      ccgtgtagc atttgtgag gaattggac ttcggtgat 
      attgttgaa aaattccga aagaagtgt tcgcagctt 
      ttatcaaaa tgtaaatgg aatgaaacc ccttttgat 
      tgctgtgag aaattcctg ccgctggag actgaatat 
      ggaatatgc tattcattc aattctctc cacacaaaa 
      aagccgtct caacatgct tcggacttt tatttcttt 
      tcgaacaga aagacgggc cccggagcg ctgtttata 
      caaactata gaggatgtt aggttatat ttccacgct 
      cgagaggat gttcctttt ataaattcg gatcccgac 
      caacgaaag gacgtcatg ctgggagaa acttacaat 
      atttctata aatataata gaaatagaa aatgacgag 
      aacgtgaag cttttaccg gtcgaaaag cgcaaatgc 
      agatttcct gaagaaaaa ccgggcgat ttaattgtt 
      cacgactat tacagttat tcgacttgt gttgtgcaa 
      tgccatgct gacgcccat atcgatttg tgcaactgc 
      acccatcat ctgatgcct gttttaaac aaggaaaaa 
      tactgcgac atcgagggt ttgaagtgt ttaactgac 
      aactttgag acagtaaat agacttcat gccaaagga 
      tttgacaag ccgggatta gtttgtgat tgcctgccg 
      tcttgcatt gagcccgaa tataaagtt gtggcagat 
      acaaaagga gttaaaagt tcccatgga ctacgatgg 
      acttag
    mRNA sequence
    • >TC033776 RA
      atatgaagc aaactttga agtatttcc ttagtatta 
      taaacttta aatggtttt tcattggtt ttggatatt 
      gaatattcc gcgagaagt cgtagccct gtgacaatg 
      atcctgtcc agattaaaa tattacgga aaaaacaca 
      actctgcac gggctaaaa tacgtagcc gacgaaaag 
      agacgtccc gtgataaga ttgacctgg atagcaatt 
      tttatcttg agcttttat ggtatgata ttagtcttc 
      aaagcttcg tataaatca tacaccgag aacgccatc 
      agtttcgtc acagaaacc gcttactta aactggaac 
      acttctttt ccagctgtc acactttgc gaaattacc 
      agttcggaa gtggcctgg aacggggat aaggaagag 
      gccgaggcc gtccagcag tttattgca gatttgata 
      ttttttacc ggcagctgc tattcttgt cacacaccg 
      tgtagcatt tgtgaggaa ttggacttc ggtgatatt 
      gttgaaaaa ttccgaaag aagtgttcg cagctttta 
      tcaaaatgt aaatggaat gaaacccct tttgattgc 
      tgtgagaaa ttcctgccg ctggagact gaatatgga 
      atatgctat tcattcaat tctctccac acaaaaaag 
      ccgtctcaa catgcttcg gacttttat ttcttttcg 
      aacagaaag acgggcccc ggagcgctg tttatacaa 
      actatagag gatgttagg ttatatttc cacgctcga 
      gaggatgtt ccttttata aattcggat cccgaccaa 
      cgaaaggac gtcatgctg ggagaaact tacaatatt 
      tctataaat ataatagaa atagaaaat gacgagaac 
      gtgaagctt ttaccggtc gaaaagcgc aaatgcaga 
      tttcctgaa gaaaaaccg ggcgattta attgttcac 
      gactattac agttattcg acttgtgtt gtgcaatgc 
      catgctgac gcccatatc gatttgtgc aactgcacc 
      catcatctg atgcctgtt ttaaacaag gaaaaatac 
      tgcgacatc gagggtttg aagtgttta actgacaac 
      tttgagaca gtaaataga cttcatgcc aaaggattt 
      gacaagccg ggattagtt tgtgattgc ctgccgtct 
      tgcattgag cccgaatat aaagttgtg gcagataca 
      aaaggagtt aaaagttcc catggacta cgatggact 
      tagtaggta agtcaggtc ctcttcgta caacacaat 
      ttacaagaa ctttagtat caattggag gaacagcag 
      gactgttca ttggggcgt ctattctaa gcat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY