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Gene information
General information
TC008904 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11521902..11526669
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008904
    iBeetle screen iB_08767

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008904 RA
      MEETLIIKT EEAPQKNEE TVVLQNIPM ESEEIPIVF 
      GELIENNSD GNLQINSVQ EEDVIHTYI IQEGEYSNI 
      EYEETTITE EKEEVEKEH EEGEEKEDE HSEVEQELD 
      ESNSMDYSE SYVEQTEEA EDDPEDHPD YADSQEFGE 
      FECDLCQRK FKTTAGLKR HITVNHAEE EDLKDPLTF 
      QLCPCCGEP MDSAHTIGD FKCEKCDKL FVQQNSLDR 
      HYSIEHCEG NAYKCVDCR KEFKQKEQL IDHMKVHPL 
      SKSIKCPDC NREFTRKYH LDRHIDQTG CNGAPKKAF 
      ACKVCNKVF HRKDNLADH LRAHAGQGK RKKLFICEY 
      CQKEFHGSA LLNIHVRTH TGEKPYPCD LCPKRFPSS 
      GAMKKHRRM HTGERPYSC KECHNRFAA KETLNRHMR 
      THTGEKPHS CQFCGKSFI QASQLRAHI FHHTGENAY 
      TCPHCNRAF NRKLRLTTH IKYMHEGAE PLTCSECDK 
      TFFRKEDLA RHFLSHTGE RPFECDVCH KSFAVKSSL 
      KIHQLTHRK EPPCSCDTC GRAFIRQDC LMRHMRARH 
      RDVLEDIMA SAEKKRLQQ QLLNAATEA VAKNQGSLT 
      ENMVWNELT LTESIKELL TILVDEACL QDFGFPDAP 
      VDKILDAVI KRCGHKPAS EEDFDYIGR MRENAKLLF 
      TVVIDDEAV KSLLNNQTV DEVILHVLR LAKAEST
    CDS sequence
    • >TC008904 RA
      atggaagag acgctaatc attaagacg gaagaggcg 
      cctcagaag aatgaggaa acggtggtt ttgcagaac 
      atccccatg gagagcgag gagattccc atcgtcttt 
      ggggagcta attgagaat aactccgat gggaatctc 
      cagattaac agcgtgcag gaggaggat gttatacac 
      acttacatc attcaggaa ggggagtat agtaacatt 
      gagtatgag gaaaccacc ataaccgaa gagaaggaa 
      gaggtggag aaggagcat gaggaaggt gaagagaaa 
      gaggatgaa cactcagaa gtcgagcaa gaacttgac 
      gaatcaaac tccatggat tactcggaa agttacgtt 
      gaacaaact gaagaagca gaagatgat ccggaggat 
      caccccgac tatgctgac tcccaagaa tttggcgag 
      tttgaatgt gatttgtgt caacggaaa tttaaaact 
      actgctggc ctcaaaaga cacatcact gtaaatcac 
      gctgaagaa gaggacctg aaggaccct ctaactttc 
      caattgtgt ccctgttgt ggtgaacca atggattcg 
      gcccacact atcggggac tttaaatgc gagaaatgt 
      gacaaactt ttcgtccag caaaacagc ctcgatcgc 
      cattacagt attgagcat tgtgagggc aatgcttac 
      aagtgtgta gattgtagg aaagaattc aagcagaaa 
      gaacagttg attgaccac atgaaagtt catcctcta 
      tccaagtcg attaaatgc cccgattgt aaccgggaa 
      tttacgcgc aagtatcat ttagacagg catattgac 
      cagactggg tgcaatggg gcgcccaaa aaggcattt 
      gcttgtaag gtttgtaac aaagtattt catcgcaaa 
      gataatctc gctgatcac ttgagagct cacgcggga 
      caagggaag aggaagaaa ctttttatt tgtgaatat 
      tgccaaaaa gaattccac ggctctgct ttacttaat 
      attcatgtg agaactcac acaggtgaa aaaccgtat 
      ccttgcgat ttgtgtcct aagagattt ccgtcaagt 
      ggcgcaatg aagaaacat agaaggatg cacactgga 
      gagagacca tattcgtgt aaagagtgc cataacaga 
      ttcgccgct aaagagacc ctcaaccgc cacatgcgc 
      acccacacc ggcgaaaag ccgcattct tgccaattc 
      tgcggcaaa tccttcatc caggcatcg caattgcgt 
      gcccacatc ttccaccac actggtgaa aacgcgtac 
      acttgccct cactgtaac cgagctttc aaccgaaaa 
      ctacgattg acaactcac attaaatac atgcacgaa 
      ggagcagag cctttaaca tgctcggaa tgcgataaa 
      acctttttc agaaaggag gacttagcg cgccacttt 
      ttatcacac actggagag cgaccgttc gaatgcgac 
      gtctgccac aaatcgttc gctgtaaaa tcctcgttg 
      aaaatccac caactgacc caccgtaaa gaacccccg 
      tgtagttgc gatacgtgc ggtcgggcg ttcattcgc 
      caggattgc ctgatgcgg catatgcga gctcgtcat 
      agggacgtt cttgaggat attatggcg agtgcagag 
      aagaagcgg ttgcagcaa cagttgttg aatgctgcg 
      acggaagcc gtggctaag aatcaagga agtttgacg 
      gagaatatg gtttggaac gagttgact ttaacagag 
      tcgattaaa gagttgttg acgattctt gtggatgag 
      gcgtgtttg caagatttt ggatttccg gatgctcca 
      gttgataag attttggat gcggtgatt aaaagatgt 
      ggacacaag ccggcctct gaggaagat tttgactac 
      attgggcgc atgagagag aacgccaag ttgctgttc 
      actgtggtt attgacgac gaagcggtc aagtcttta 
      ttgaataat cagacggtg gatgaagtg atattgcac 
      gtcttgagg ttagcaaag gctgagagt acttag
    mRNA sequence
    • >TC008904 RA
      taacatttg gacgcgcct ggaaaatag tccgcctcc 
      attttatgt atacgcgtg ataaaacgg ttgacgacg 
      tttcagggg ccaaatgga agagacgct aatcattaa 
      gacggaaga ggcgcctca gaagaatga ggaaacggt 
      ggttttgca gaacatccc catggagag cgaggagat 
      tcccatcgt ctttgggga gctaattga gaataactc 
      cgatgggaa tctccagat taacagcgt gcaggagga 
      ggatgttat acacactta catcattca ggaagggga 
      gtatagtaa cattgagta tgaggaaac caccataac 
      cgaagagaa ggaagaggt ggagaagga gcatgagga 
      aggtgaaga gaaagagga tgaacactc agaagtcga 
      gcaagaact tgacgaatc aaactccat ggattactc 
      ggaaagtta cgttgaaca aactgaaga agcagaaga 
      tgatccgga ggatcaccc cgactatgc tgactccca 
      agaatttgg cgagtttga atgtgattt gtgtcaacg 
      gaaatttaa aactactgc tggcctcaa aagacacat 
      cactgtaaa tcacgctga agaagagga cctgaagga 
      ccctctaac tttccaatt gtgtccctg ttgtggtga 
      accaatgga ttcggccca cactatcgg ggactttaa 
      atgcgagaa atgtgacaa acttttcgt ccagcaaaa 
      cagcctcga tcgccatta cagtattga gcattgtga 
      gggcaatgc ttacaagtg tgtagattg taggaaaga 
      attcaagca gaaagaaca gttgattga ccacatgaa 
      agttcatcc tctatccaa gtcgattaa atgccccga 
      ttgtaaccg ggaatttac gcgcaagta tcatttaga 
      caggcatat tgaccagac tgggtgcaa tggggcgcc 
      caaaaaggc atttgcttg taaggtttg taacaaagt 
      atttcatcg caaagataa tctcgctga tcacttgag 
      agctcacgc gggacaagg gaagaggaa gaaactttt 
      tatttgtga atattgcca aaaagaatt ccacggctc 
      tgctttact taatattca tgtgagaac tcacacagg 
      tgaaaaacc gtatccttg cgatttgtg tcctaagag 
      atttccgtc aagtggcgc aatgaagaa acatagaag 
      gatgcacac tggagagag accatattc gtgtaaaga 
      gtgccataa cagattcgc cgctaaaga gaccctcaa 
      ccgccacat gcgcaccca caccggcga aaagccgca 
      ttcttgcca attctgcgg caaatcctt catccaggc 
      atcgcaatt gcgtgccca catcttcca ccacactgg 
      tgaaaacgc gtacacttg ccctcactg taaccgagc 
      tttcaaccg aaaactacg attgacaac tcacattaa 
      atacatgca cgaaggagc agagccttt aacatgctc 
      ggaatgcga taaaacctt tttcagaaa ggaggactt 
      agcgcgcca ctttttatc acacactgg agagcgacc 
      gttcgaatg cgacgtctg ccacaaatc gttcgctgt 
      aaaatcctc gttgaaaat ccaccaact gacccaccg 
      taaagaacc cccgtgtag ttgcgatac gtgcggtcg 
      ggcgttcat tcgccagga ttgcctgat gcggcatat 
      gcgagctcg tcataggga cgttcttga ggatattat 
      ggcgagtgc agagaagaa gcggttgca gcaacagtt 
      gttgaatgc tgcgacgga agccgtggc taagaatca 
      aggaagttt gacggagaa tatggtttg gaacgagtt 
      gactttaac agagtcgat taaagagtt gttgacgat 
      tcttgtgga tgaggcgtg tttgcaaga ttttggatt 
      tccggatgc tccagttga taagatttt ggatgcggt 
      gattaaaag atgtggaca caagccggc ctctgagga 
      agattttga ctacattgg gcgcatgag agagaacgc 
      caagttgct gttcactgt ggttattga cgacgaagc 
      ggtcaagtc tttattgaa taatcagac ggtggatga 
      agtgatatt gcacgtctt gaggttagc aaaggctga 
      gagtactta gaactagac gtttagaat atttagggc 
      gtatttttg tagttagtt acagtttct tcaaatgaa 
      tgtatgtac agtcttact tttctttta ttaattatt 
      atatgttta aaatcacaa tgatgtgtt tatattcac 
      cattataaa tttacttgt ttactgatt ttttatttg 
      ggtcgtatc atgtatggg agat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY