Status
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Gene information
General information
TC009935 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11593137..11604510
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009935
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009935 RA
      MTTLELYTK GAKVWIPHP EKVWEGAEL EEDYKTNKK 
      SLEVVTENN EKKTLTIKS ESDLPCLRN PSILVGEND 
      LTSLSYLHE PAVLYNLQV RFCQNRYIY TYCGIVLVA 
      INPYDDLPI YDVDTIQTY RGQAMGDLD PHIFAVAEE 
      AYTKLEREQ RDQSIIVSG ESGAGKTVS AKYAMRYFA 
      TVGGSATET QVEKKVLAS SPIMEAIGN AKTTRNDNS 
      SRFGKFIEL QFNKQFHIS GASMRTYLL EKSRVVFQA 
      PDERNYHIF YQLCSARDQ FPHLHLAHQ DSFYYLNQG 
      ESPNVDGVD DLQTFEETL YALNLLGFK KSDLDDMFK 
      IIASVLHLG NIKFVESII STENEQDQE GCSILGTDS 
      HLKILAELL EIDSKEMQQ WLCTRKIVS MREVFLKPM 
      SVEDAQAAR DALAKHIYA ELFNWIVLV INKALESDI 
      PRHKFIGVL DIYGFETFE TNSFEQFCI NYANEKLQQ 
      QFNLHVFKL EQEEYIKEG IEWKMIDFY DNQPCIDLI 
      ETKLGILDL LDEECRMPR GTDSSWTEK LYSKCAKYS 
      HFGKARFGT SAFTINHFA DKVQYESNG FLEKNRDTV 
      IEEQISVVK RSKNDLVRR LFGDDSQKL AVPGAKLKV 
      ISAKPAPTS QKTHKKSVG SQFRDSLNM LMTTLNATT 
      PHYVRCIKP NDSKKPFEY NPKRAVQQL RACGVLETI 
      RISAAGFPS RWTYVDFFY RYRVLCKFK DIKRNDMQL 
      TCQNILNQY IKNKDMFQF GKTKIFFRA GQVAYLEKL 
      RADKLKACC IMMQKTIRA FIWRKKYLR IKRCTINIQ 
      RYGRGYLAR KLAENLRRE KAAKTLQRY VRGWVKRVQ 
      YQRLKACVT GIQRYARGY LARRRYMQL RYNAKALVI 
      QRYVRGYLA RRSALAYKR KIVICQAAI RRFLARRLY 
      KKLRIEARS IEHVKKLNK GLENKIISL QQKIDEITK 
      QNAELMTYK AGVNELKNK LTAFKANEA EIKNLNARL 
      IEKDKVIDK LKEDLKLER DEKIDLINE HEKYRQETQ 
      KQRDLWTQE TNKLRKELD NINEIVKMN QKGAEENLK 
      VRLEEEKML ILNEQDSDR EAYQKLLQE YHCLEQHCE 
      ELEKQLNNQ SQNQSSHRR NVSDLSSID SFVINSDIA 
      EDHGYGSVR STTTSSSQR EKLENIDWK CEGNVDSQT 
      PSTSSSDTK HESENNKVD VGLVLKLQH KLAEVEREK 
      LRMQKRLDE LDMSPRTEK AENAARDSI KISELELVN 
      SNLKAQLME LQNSIIEGT GSSKIHEQM QILHSELER 
      KTEEIIQLK SVLADQTNN MKSLVNSNA RLGAYINED 
      GELALAYET QKTINKQLE LELQDEKSK YKAHEREYK 
      LEIEKLRED NERQQRLLS ANLTNTPQT QSEAFMQHE 
      ITRLTIENL DLHDKNDTL TETVRKLKK QIKLMAKKL 
      KEAGLDMDD NFNEITDNE PNKTNVNKH TRALPSIRK 
      KERDYLGMF SYSSGEENT IMRQLVIDL KPRTAVTLL 
      PGLPAYIVF MCIRHTDYV NDEDKVKAL LSAFTNSVK 
      KVIKKRHED FETMALWLS NTLRLVHNM KQYSGDRAF 
      QAKNTPKQN EQCLRNFDL SEYRQVLSD IAVWIYQGL 
      IRKFAEKIQ PLVIPAILE HEEIPGISG NKPSGFRGR 
      SSSVATSPE PSQKPTTAV LLELTNHHK ILSFYGVDP 
      EVISQIFKQ IFYFLCATS LNNLLLRQE LCHWSKGFQ 
      IRHNLSHFE MWTREKGLD EASIQSTLQ PIIQAAHLL 
      QARKTEEDV ASVCEMCSA LTPLQICKI LNLYKPVDE 
      FEQHVPPSF ISKVKAKLQ ERNPTSEQQ TLLMDVKYH 
      FPVRFPFNP SVICLEDIE IPEVLKLPQ LEKL
    CDS sequence
    • >TC009935 RA
      atgactacc ttggagcta tatacaaag ggtgccaag 
      gtatggata cctcaccct gaaaaagta tgggaaggc 
      gctgaactt gaagaagac tacaaaacg aacaaaaag 
      tcacttgaa gtagtaaca gaaaacaac gaaaagaaa 
      acccttacg atcaaatca gaatcggat ttaccctgt 
      ctgaggaac ccttcaatt ttagtcggg gaaaatgac 
      cttacatcc ctctcctac ttacacgag cccgccgta 
      ctttacaat ttacaagta cggttctgc caaaatcga 
      tacatttac acctattgt gggattgtt ttggtggca 
      attaatccc tacgatgat cttcctatt tatgacgtc 
      gacaccata caaacgtat agaggtcaa gcgatgggt 
      gacctagat cctcacata ttcgccgta gccgaagaa 
      gcgtacacg aaactagaa agagagcaa agggaccaa 
      agcataatc gtatcagga gaaagcgga gccggtaaa 
      acggtttcc gcgaaatac gcaatgaga tacttcgcg 
      actgtagga ggaagtgcg accgaaacc caagtcgaa 
      aaaaaagtc ctagctagt agtcctatc atggaagcc 
      atcggaaac gcgaaaact acaagaaac gataacagt 
      tcgcgattt ggaaagttt atagaactc cagtttaat 
      aagcaattt catattagc ggagcgtcg atgagaacg 
      tacctcttg gaaaaatcg cgagtcgtc tttcaggct 
      cccgatgaa cggaattac catattttc taccaattg 
      tgttccgcg agagaccaa tttccacac ttgcatctc 
      gctcaccaa gatagtttt tattattta aatcaagga 
      gaaagtcca aatgtggac ggagtcgac gatttacaa 
      accttcgaa gagacgctc tatgcgttg aatctttta 
      gggtttaag aaatcggat ctggacgat atgtttaaa 
      attatagcc tccgtcttg catttggga aatatcaag 
      tttgttgaa tcgatcatt tccacggaa aacgaacaa 
      gaccaggag ggttgtagc atactgggc actgactca 
      catttgaaa atcttagcg gaattactc gagatcgat 
      tcaaaagaa atgcagcag tggctctgt acccgaaaa 
      atcgtctct atgagagag gtcttcctg aaaccaatg 
      tccgtcgaa gacgcgcaa gctgcccgt gatgcttta 
      gcaaaacac atttatgca gaattgttc aactggatc 
      gtgttagtt ataaacaaa gctttagaa tccgacatt 
      cctagacat aaatttatc ggagtcctc gatatctac 
      ggtttcgaa accttcgaa accaattcc tttgaacaa 
      ttttgtatc aattacgcc aacgaaaaa ctccaacaa 
      caattcaac ctccacgtg tttaaactg gagcaggag 
      gaatacatt aaagaagga attgaatgg aaaatgatt 
      gatttctac gacaaccaa ccctgcatc gacctcatc 
      gaaacaaaa ttagggatt ttagatttg ctagacgag 
      gagtgtcgg atgccgcga ggtactgac agttcctgg 
      acggagaaa ctgtactca aagtgcgca aagtattcc 
      cacttcggc aaggcgcgt ttcggcact tctgccttc 
      accatcaat cactttgcc gataaagtc caatacgaa 
      agcaacggt tttctggag aaaaaccgc gacaccgta 
      atcgaggag caaatctcg gtggtgaaa aggagcaaa 
      aacgatcta gtccgccgg cttttcggc gacgattct 
      caaaaattg gccgttccg ggagccaaa ctcaaagtg 
      atctcggcc aaacctgcc ccaacctcg caaaaaacc 
      cacaagaaa tcggtgggg tcccagttt agagactcg 
      cttaatatg ctaatgacc acgttgaac gccacaacc 
      ccgcattac gtccggtgt attaagcca aacgattcg 
      aaaaaaccg tttgagtat aacccgaaa cgggcggtg 
      caacaactg cgagcttgt ggcgtgttg gagacgatc 
      aggatttcg gcagccggg tttccgagt cggtggact 
      tacgttgat ttcttctac cggtaccga gtcttgtgc 
      aagtttaaa gatatcaaa agaaacgac atgcagctc 
      acttgccag aatatactg aaccagtat attaaaaac 
      aaggatatg tttcaattc gggaaaact aagattttt 
      ttccgggcc ggacaagtc gcctatttg gagaaatta 
      agagccgat aaactcaaa gcctgttgc atcatgatg 
      caaaaaaca atccgggcg ttcatttgg aggaaaaag 
      tacctccga atcaagcgc tgcactatc aatatacaa 
      cggtatgga cggggttat ttggcccga aaactggcc 
      gaaaatttg cggcgcgag aaagccgca aagactttg 
      cagagatat gttcgagga tgggtgaaa cgtgtccaa 
      tatcaaaga ctgaaggcc tgtgtgact ggcattcaa 
      aggtatgcc agagggtat ttagcgaga cggcggtac 
      atgcaatta cggtacaac gccaaagct ctggtaata 
      cagcgatat gtaagaggg tatttagca cggagaagt 
      gccctggct tacaagcgg aaaatcgtc atatgccaa 
      gctgcgatt cgccgcttt ttggctaga agattatac 
      aagaaactc agaatcgaa gcaagatca atcgaacac 
      gtcaagaaa cttaacaaa ggcttggag aacaagatc 
      atcagtttg cagcaaaag attgacgaa attacgaaa 
      caaaacgcg gaattgatg acttataag gccggagtt 
      aacgagctg aagaataaa ttgacggcg ttcaaagcg 
      aatgaggct gaaattaag aacttgaac gctcgtctg 
      attgagaaa gacaaagtc attgataaa ttgaaggag 
      gatcttaag ttggaacgg gacgaaaaa attgacttg 
      attaacgaa catgagaaa tatcgacaa gaaacgcaa 
      aagcagcgt gatttatgg acgcaggaa acaaacaaa 
      ttgcgtaaa gagctagac aatatcaac gagatcgtt 
      aaaatgaac cagaaggga gccgaagaa aatctcaaa 
      gtgcgactc gaagaggag aaaatgttg attttgaat 
      gagcaagat agtgatagg gaagcctac cagaagctt 
      ttacaagag taccattgc ttggaacaa cactgcgaa 
      gaactagag aagcagttg aacaatcag agccaaaac 
      caaagttcc caccgaaga aatgtgagc gatcttagc 
      agcattgac agttttgtt atcaacagc gatattgct 
      gaagaccac ggttacggt tccgtgaga tcgaccaca 
      acttcgtcg tcacaaaga gaaaaactt gagaatatc 
      gactggaaa tgtgaagga aatgtagat agtcaaaca 
      ccaagtaca agtagcagt gacaccaaa catgaatcc 
      gaaaacaat aaagttgac gtcggtttg gtccttaaa 
      ctccagcac aaacttgcc gaagttgag cgtgaaaaa 
      ctgcgaatg cagaaacgc ctagacgag ttggacatg 
      tctccacga accgaaaag gctgaaaac gcggcacga 
      gattccatt aaaatctcc gaacttgaa ctagttaac 
      agcaaccta aaggcgcaa ctgatggaa ctgcaaaac 
      agcattatt gaaggaact ggcagttcg aaaatacac 
      gaacagatg caaatatta cacagcgaa ctggaacga 
      aaaactgag gaaataatc caactcaag agtgtcttg 
      gccgaccag actaacaac atgaaatca ctcgtgaac 
      tcaaatgca cgattagga gcgtacatt aatgaagac 
      ggagagtta gcactggct tacgaaacc caaaaaaca 
      atcaataag caactcgaa ttggaatta caagacgag 
      aaatcaaaa tacaaagct cacgaacgg gaatacaag 
      ctagagatt gaaaaatta agggaagat aatgaacgc 
      caacaacga cttttatcc gcaaatctc accaacacg 
      ccacaaaca caaagcgaa gcttttatg caacacgaa 
      attacaaga ttaactata gaaaatctt gacttacac 
      gacaaaaac gatactttg accgagaca gtccgaaaa 
      cttaagaaa caaatcaag ttaatggcc aagaaactt 
      aaagaagca gggctggat atggacgat aattttaac 
      gaaattacc gacaacgaa cctaacaaa acgaatgta 
      aacaagcac actcgggcc ctgccgtcg atcagaaag 
      aaagaaaga gattattta ggcatgttc tcgtattct 
      agtggggag gaaaatacg atcatgcgg caacttgtt 
      attgatttg aagcctagg actgcggtc actttgctt 
      cctggcttg ccagcttac atagttttc atgtgtata 
      agacacacg gattacgtc aacgacgaa gataaagtg 
      aaagccttg ctctccgcg tttacaaac tctgtaaaa 
      aaagtgata aagaagcga cacgaagat ttcgaaacc 
      atggcttta tggctttca aacaccttg agattagtg 
      cacaatatg aaacagtac agcggtgac cgagctttc 
      caggctaaa aatacgcca aaacaaaac gagcagtgt 
      ttgcgaaat ttcgattta tcggaatat cgacaagtg 
      ctttcagat attgctgtg tggatttac cagggactg 
      atccgaaag tttgccgaa aaaatccaa ccacttgtc 
      atacccgcc attctggag cacgaggaa atcccggga 
      attagcggg aataaacct agtggcttc agagggagg 
      agcagcagt gtagcgaca agccctgaa cccagccaa 
      aaaccaacg accgcggtt ttactggaa ttaacaaac 
      caccacaag attttaagt ttctatggc gttgacccg 
      gaagtaatt tcccaaatt ttcaaacaa atattttac 
      tttttatgc gcgacaagt ctcaataat ttgctccta 
      cggcaagag ttgtgccac tggtcgaaa ggtttccaa 
      attcggcac aacttatcc cacttcgag atgtggaca 
      cgagaaaaa ggccttgat gaggcttca atccagtca 
      actttacag cccataatt caagcggcc catttatta 
      caagcgcgt aaaaccgaa gaagacgtt gcaagtgtg 
      tgtgaaatg tgtagcgct ttaacgcca ctacaaatt 
      tgtaaaata ctgaatttg tataaacct gtagatgaa 
      tttgaacaa cacgtgccc ccgtcattt attagcaaa 
      gttaaagcc aagttacag gagcggaat cccacttcg 
      gaacaacaa acgctgctc atggacgta aagtatcac 
      ttcccagtc cgattcccg ttcaatcct tccgtcata 
      tgtttggaa gacattgaa attccagaa gttttaaaa 
      ttgcctcag ttggaaaaa ctataa
    mRNA sequence
    • >TC009935 RA
      gtacctaac cggaattca ttcctagtg atccccaga 
      gggcgctag caattcttt tgagcgcca aatcgccaa 
      atccatact tttacacat gactcctag tgatcgaaa 
      taagccacg cagtgatta gtgcgcatg gtgcgccgt 
      gtaggtgca ggttgtgtg ttttactac cattataat 
      acacgagag ggttttcta tttgttggc gggttgtgc 
      tggctccgg attattcgt ttgattact ttttttttg 
      atgatttat gaacaagat cagtgaaaa cggtgcaaa 
      atgactacc ttggagcta tatacaaag ggtgccaag 
      gtatggata cctcaccct gaaaaagta tgggaaggc 
      gctgaactt gaagaagac tacaaaacg aacaaaaag 
      tcacttgaa gtagtaaca gaaaacaac gaaaagaaa 
      acccttacg atcaaatca gaatcggat ttaccctgt 
      ctgaggaac ccttcaatt ttagtcggg gaaaatgac 
      cttacatcc ctctcctac ttacacgag cccgccgta 
      ctttacaat ttacaagta cggttctgc caaaatcga 
      tacatttac acctattgt gggattgtt ttggtggca 
      attaatccc tacgatgat cttcctatt tatgacgtc 
      gacaccata caaacgtat agaggtcaa gcgatgggt 
      gacctagat cctcacata ttcgccgta gccgaagaa 
      gcgtacacg aaactagaa agagagcaa agggaccaa 
      agcataatc gtatcagga gaaagcgga gccggtaaa 
      acggtttcc gcgaaatac gcaatgaga tacttcgcg 
      actgtagga ggaagtgcg accgaaacc caagtcgaa 
      aaaaaagtc ctagctagt agtcctatc atggaagcc 
      atcggaaac gcgaaaact acaagaaac gataacagt 
      tcgcgattt ggaaagttt atagaactc cagtttaat 
      aagcaattt catattagc ggagcgtcg atgagaacg 
      tacctcttg gaaaaatcg cgagtcgtc tttcaggct 
      cccgatgaa cggaattac catattttc taccaattg 
      tgttccgcg agagaccaa tttccacac ttgcatctc 
      gctcaccaa gatagtttt tattattta aatcaagga 
      gaaagtcca aatgtggac ggagtcgac gatttacaa 
      accttcgaa gagacgctc tatgcgttg aatctttta 
      gggtttaag aaatcggat ctggacgat atgtttaaa 
      attatagcc tccgtcttg catttggga aatatcaag 
      tttgttgaa tcgatcatt tccacggaa aacgaacaa 
      gaccaggag ggttgtagc atactgggc actgactca 
      catttgaaa atcttagcg gaattactc gagatcgat 
      tcaaaagaa atgcagcag tggctctgt acccgaaaa 
      atcgtctct atgagagag gtcttcctg aaaccaatg 
      tccgtcgaa gacgcgcaa gctgcccgt gatgcttta 
      gcaaaacac atttatgca gaattgttc aactggatc 
      gtgttagtt ataaacaaa gctttagaa tccgacatt 
      cctagacat aaatttatc ggagtcctc gatatctac 
      ggtttcgaa accttcgaa accaattcc tttgaacaa 
      ttttgtatc aattacgcc aacgaaaaa ctccaacaa 
      caattcaac ctccacgtg tttaaactg gagcaggag 
      gaatacatt aaagaagga attgaatgg aaaatgatt 
      gatttctac gacaaccaa ccctgcatc gacctcatc 
      gaaacaaaa ttagggatt ttagatttg ctagacgag 
      gagtgtcgg atgccgcga ggtactgac agttcctgg 
      acggagaaa ctgtactca aagtgcgca aagtattcc 
      cacttcggc aaggcgcgt ttcggcact tctgccttc 
      accatcaat cactttgcc gataaagtc caatacgaa 
      agcaacggt tttctggag aaaaaccgc gacaccgta 
      atcgaggag caaatctcg gtggtgaaa aggagcaaa 
      aacgatcta gtccgccgg cttttcggc gacgattct 
      caaaaattg gccgttccg ggagccaaa ctcaaagtg 
      atctcggcc aaacctgcc ccaacctcg caaaaaacc 
      cacaagaaa tcggtgggg tcccagttt agagactcg 
      cttaatatg ctaatgacc acgttgaac gccacaacc 
      ccgcattac gtccggtgt attaagcca aacgattcg 
      aaaaaaccg tttgagtat aacccgaaa cgggcggtg 
      caacaactg cgagcttgt ggcgtgttg gagacgatc 
      aggatttcg gcagccggg tttccgagt cggtggact 
      tacgttgat ttcttctac cggtaccga gtcttgtgc 
      aagtttaaa gatatcaaa agaaacgac atgcagctc 
      acttgccag aatatactg aaccagtat attaaaaac 
      aaggatatg tttcaattc gggaaaact aagattttt 
      ttccgggcc ggacaagtc gcctatttg gagaaatta 
      agagccgat aaactcaaa gcctgttgc atcatgatg 
      caaaaaaca atccgggcg ttcatttgg aggaaaaag 
      tacctccga atcaagcgc tgcactatc aatatacaa 
      cggtatgga cggggttat ttggcccga aaactggcc 
      gaaaatttg cggcgcgag aaagccgca aagactttg 
      cagagatat gttcgagga tgggtgaaa cgtgtccaa 
      tatcaaaga ctgaaggcc tgtgtgact ggcattcaa 
      aggtatgcc agagggtat ttagcgaga cggcggtac 
      atgcaatta cggtacaac gccaaagct ctggtaata 
      cagcgatat gtaagaggg tatttagca cggagaagt 
      gccctggct tacaagcgg aaaatcgtc atatgccaa 
      gctgcgatt cgccgcttt ttggctaga agattatac 
      aagaaactc agaatcgaa gcaagatca atcgaacac 
      gtcaagaaa cttaacaaa ggcttggag aacaagatc 
      atcagtttg cagcaaaag attgacgaa attacgaaa 
      caaaacgcg gaattgatg acttataag gccggagtt 
      aacgagctg aagaataaa ttgacggcg ttcaaagcg 
      aatgaggct gaaattaag aacttgaac gctcgtctg 
      attgagaaa gacaaagtc attgataaa ttgaaggag 
      gatcttaag ttggaacgg gacgaaaaa attgacttg 
      attaacgaa catgagaaa tatcgacaa gaaacgcaa 
      aagcagcgt gatttatgg acgcaggaa acaaacaaa 
      ttgcgtaaa gagctagac aatatcaac gagatcgtt 
      aaaatgaac cagaaggga gccgaagaa aatctcaaa 
      gtgcgactc gaagaggag aaaatgttg attttgaat 
      gagcaagat agtgatagg gaagcctac cagaagctt 
      ttacaagag taccattgc ttggaacaa cactgcgaa 
      gaactagag aagcagttg aacaatcag agccaaaac 
      caaagttcc caccgaaga aatgtgagc gatcttagc 
      agcattgac agttttgtt atcaacagc gatattgct 
      gaagaccac ggttacggt tccgtgaga tcgaccaca 
      acttcgtcg tcacaaaga gaaaaactt gagaatatc 
      gactggaaa tgtgaagga aatgtagat agtcaaaca 
      ccaagtaca agtagcagt gacaccaaa catgaatcc 
      gaaaacaat aaagttgac gtcggtttg gtccttaaa 
      ctccagcac aaacttgcc gaagttgag cgtgaaaaa 
      ctgcgaatg cagaaacgc ctagacgag ttggacatg 
      tctccacga accgaaaag gctgaaaac gcggcacga 
      gattccatt aaaatctcc gaacttgaa ctagttaac 
      agcaaccta aaggcgcaa ctgatggaa ctgcaaaac 
      agcattatt gaaggaact ggcagttcg aaaatacac 
      gaacagatg caaatatta cacagcgaa ctggaacga 
      aaaactgag gaaataatc caactcaag agtgtcttg 
      gccgaccag actaacaac atgaaatca ctcgtgaac 
      tcaaatgca cgattagga gcgtacatt aatgaagac 
      ggagagtta gcactggct tacgaaacc caaaaaaca 
      atcaataag caactcgaa ttggaatta caagacgag 
      aaatcaaaa tacaaagct cacgaacgg gaatacaag 
      ctagagatt gaaaaatta agggaagat aatgaacgc 
      caacaacga cttttatcc gcaaatctc accaacacg 
      ccacaaaca caaagcgaa gcttttatg caacacgaa 
      attacaaga ttaactata gaaaatctt gacttacac 
      gacaaaaac gatactttg accgagaca gtccgaaaa 
      cttaagaaa caaatcaag ttaatggcc aagaaactt 
      aaagaagca gggctggat atggacgat aattttaac 
      gaaattacc gacaacgaa cctaacaaa acgaatgta 
      aacaagcac actcgggcc ctgccgtcg atcagaaag 
      aaagaaaga gattattta ggcatgttc tcgtattct 
      agtggggag gaaaatacg atcatgcgg caacttgtt 
      attgatttg aagcctagg actgcggtc actttgctt 
      cctggcttg ccagcttac atagttttc atgtgtata 
      agacacacg gattacgtc aacgacgaa gataaagtg 
      aaagccttg ctctccgcg tttacaaac tctgtaaaa 
      aaagtgata aagaagcga cacgaagat ttcgaaacc 
      atggcttta tggctttca aacaccttg agattagtg 
      cacaatatg aaacagtac agcggtgac cgagctttc 
      caggctaaa aatacgcca aaacaaaac gagcagtgt 
      ttgcgaaat ttcgattta tcggaatat cgacaagtg 
      ctttcagat attgctgtg tggatttac cagggactg 
      atccgaaag tttgccgaa aaaatccaa ccacttgtc 
      atacccgcc attctggag cacgaggaa atcccggga 
      attagcggg aataaacct agtggcttc agagggagg 
      agcagcagt gtagcgaca agccctgaa cccagccaa 
      aaaccaacg accgcggtt ttactggaa ttaacaaac 
      caccacaag attttaagt ttctatggc gttgacccg 
      gaagtaatt tcccaaatt ttcaaacaa atattttac 
      tttttatgc gcgacaagt ctcaataat ttgctccta 
      cggcaagag ttgtgccac tggtcgaaa ggtttccaa 
      attcggcac aacttatcc cacttcgag atgtggaca 
      cgagaaaaa ggccttgat gaggcttca atccagtca 
      actttacag cccataatt caagcggcc catttatta 
      caagcgcgt aaaaccgaa gaagacgtt gcaagtgtg 
      tgtgaaatg tgtagcgct ttaacgcca ctacaaatt 
      tgtaaaata ctgaatttg tataaacct gtagatgaa 
      tttgaacaa cacgtgccc ccgtcattt attagcaaa 
      gttaaagcc aagttacag gagcggaat cccacttcg 
      gaacaacaa acgctgctc atggacgta aagtatcac 
      ttcccagtc cgattcccg ttcaatcct tccgtcata 
      tgtttggaa gacattgaa attccagaa gttttaaaa 
      ttgcctcag ttggaaaaa ctataaata attctagca 
      aatatttta accaaactc acgtttgca gtgtaaata 
      atgtcaact tagtcac
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