Status
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Gene information
General information
TC010857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 5410864..5497670
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010857
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08763

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010857 RA
      MKLFLLLLL LFELGAQNV PLDNIRHSR SRTRLLRGR 
      RHGDDVRED FTSDNVLEV PSATESLFF GDDSGRNRT 
      SLSVEEPPT SKSSARKVT VGSNFNLHG NSSWQHVDK 
      SMLYMGERA LNQIDNKKK NKTVSGKEK ASPGESPIN 
      VHQGFDEDV EISSEKAAT ESARSGHVD IVTRFLRIV 
      ESQHLLGEN CTAGTDLNL GEGVVDRYA QERFRVEAD 
      VAVNRANML TRLWKYADP DVVMSEYLL HASVFSMVE 
      FDNDIFAAG NCYDQYQYK DYWLFCPYA YRLPEGTIL 
      VKDLAVEYK YLSNTSEWF YIARKNAEN VIRNYNQFS 
      KGYNTYTYN ESAHTERVP DEILSVRYE DGKWSKPYY 
      DCGGGNIWM LTYTVPFFG YSNNTYYFK GTSGIDIDL 
      RRVDIDQCP LPKGSTQLN IFAASDKCK KRTTECIPL 
      SGLGFRRGS YKCICKRGF FFPDTKADR RYYNGTVIE 
      EEYEKLMLG ETSQYAKAG IFECLSCPE GCETCEDDR 
      PCVVSLNWV MRTVILVLS CVIICCLPI VVVFTWKYG 
      NVKVVRAAS PVLLCFITL GAFLIYCTM IVMYPRPNM 
      YTCIARVWL REIGFSLTY GALMLKTWR ISVIFRVRS 
      AKAVKITDM NLLKRLGVI VAIFTVFLA IRTLVAPPV 
      VIVGRTADD LKAYLCRTD WWDHSFTTL EVMFLVWGI 
      RLCIVVRKA PSEFNESRF ISMAIYNEF LLSVFLNIS 
      MLFLQKPAN PDLLYIIFF CHTQLTVTL LLCLIFGSK 
      AYMVFKHHD QSDDSSGMM SKTPTSKFL GKARAGNSQ 
      YTSTNSSVG GLEYSKYSE QDVQEEFLR LYTQLELLK 
      EKNMRIGNR HLASKITAM QDAARSHDH VQSENPSRP 
      LGKLNNTLD VVPEKSGND VSTEDDAPV KIKRQSVSF 
      ASETEASGD PAVKDNNSC SKIVEEVAP GGAAASTEL 
      KSKDERNES PAADRNGRY LKSGHARTH AIVINLDDK 
      SRFTEEVTV
    CDS sequence
    • >TC010857 RA
      atgaagtta tttttgttg ttgttgctg ttgtttgag 
      ttgggagcg caaaatgtt cctttggac aatataagg 
      cacagtcgg tcgcggacg cgcttgctg aggggaaga 
      cggcatggg gatgatgtc cgggaagat ttcacgtct 
      gataatgtt ctcgaggtt ccttcggcg acggaaagc 
      ttgtttttt ggtgatgat tccgggaga aatcggact 
      agtctgtct gtggaggaa cctccgacg agcaaatcg 
      agcgcgagg aaggtgacg gtaggttcg aatttcaac 
      ctccatgga aacagcagt tggcagcat gtggataag 
      agcatgttg tacatgggt gagcgggcg cttaatcaa 
      atcgataac aaaaagaaa aataagaca gtgagtggt 
      aaggagaag gcgagcccc ggagagtca ccgataaac 
      gtgcatcag ggttttgac gaagatgtg gaaatatca 
      tcggaaaag gcagccacc gaaagtgct cgtagcggc 
      cacgtagac atcgttact cgtttttta cgcatcgtg 
      gagtcccag catttgttg ggagagaac tgtacggcc 
      ggcacggac ctgaatttg ggcgaagga gttgtagat 
      cgttatgca caagaaaga tttcgggtg gaggcagac 
      gtggccgta aatagggct aatatgttg actcggctg 
      tggaaatac gccgatcct gatgttgtt atgtcggag 
      tatttattg cacgcgtcc gttttttcc atggttgaa 
      ttcgataat gacatcttt gctgcggga aactgctat 
      gaccagtat cagtacaaa gactattgg ctattctgc 
      ccgtatgct tatcggctg cccgaaggg acaatcctc 
      gtgaaagac ttggccgtt gagtacaaa tatctcagc 
      aacacatcg gaatggttc tacatcgcc cgaaaaaat 
      gccgagaac gtcatcaga aactacaat caattcagt 
      aaaggttac aatacttac acatacaat gaaagtgct 
      cacacggag cgagttccc gacgagatt ttgtcagtg 
      cgttacgaa gatggaaaa tggtcaaag ccttactac 
      gactgtggt ggcggaaac atctggatg ctcacttac 
      actgttccg ttttttggc tattcgaac aatacgtac 
      tacttcaag ggaactagt ggaattgat atagatctg 
      cggcgggtt gatatcgac caatgccct ttgccgaag 
      ggcagcaca cagctcaac attttcgcg gcttcagac 
      aaatgcaaa aagcgcact acggaatgt attccgctg 
      tcaggactg ggcttcagg cggggtagt tacaagtgt 
      atatgcaaa cgcggcttt ttctttccg gacaccaaa 
      gcggacagg cgctactat aatggaacc gtgatcgaa 
      gaggaatat gaaaaactg atgttgggc gaaaccagt 
      cagtacgcg aaagctgga attttcgaa tgtctctca 
      tgtcctgaa ggatgtgaa acctgtgag gacgaccga 
      ccatgtgtg gtttctctc aactgggta atgcgaacg 
      gtcattctc gtcctttcc tgtgtcatc atctgctgt 
      ttacccatc gttgtcgtc tttacctgg aaatacggg 
      aatgtgaag gtggtacgt gccgccagt cctgttcta 
      ttatgcttc ataactttg ggagctttc ctcatatat 
      tgtacgatg attgtgatg taccctcgt cctaatatg 
      tacacgtgc atagcacga gtgtggctt agagaaatt 
      ggcttttca ttaacctac ggagctttg atgctgaag 
      acgtggcga atttccgtc atcttccgc gttcgttca 
      gctaaggcc gtcaagatt acagacatg aatttgcta 
      aagcggctt ggtgttata gttgccatt tttactgtt 
      tttctggct attcgaact cttgttgct ccgccagtc 
      gtcatcgtc ggccgaaca gctgatgac cttaaggcc 
      tatttgtgc cgcactgat tggtgggac cactcattc 
      acgacactc gaagtcatg tttctggtt tggggaata 
      cgactgtgc atcgtcgta cggaaagct ccatccgag 
      tttaatgaa agtcgcttt atttccatg gccatttat 
      aatgaattc ctactatct gtctttctc aatatatca 
      atgttgttc ctgcaaaaa cctgctaac ccagacctg 
      ctctacatt attttcttc tgtcatacg cagctcacc 
      gtaacatta cttctgtgc ctcatattc ggaagcaag 
      gcgtatatg gtgtttaag caccatgac caaagcgac 
      gacagctct ggtatgatg tcaaagacg ccgacttca 
      aaatttcta gggaaagcg cgtgcaggg aattcgcaa 
      tatacaagt acgaactcg tcagttggc ggtttagag 
      tattcaaaa tattcggag caagatgtc caggaagag 
      ttcctgcgt ttgtacacg cagctggag ttgttgaaa 
      gaaaaaaat atgcggatt gggaatagg catttggcg 
      tctaagata accgcgatg caggatgct gcgaggagt 
      catgatcat gtacaatcg gaaaatcca tccagaccc 
      cttgggaaa ttaaataat acactggat gtagttccg 
      gagaaatcg ggcaacgac gtaagtaca gaggacgac 
      gcacccgtt aaaattaaa cgtcaaagt gtttcattc 
      gcgtcggaa actgaagcc agtggcgat cctgcagtt 
      aaagataat aatagttgt agtaagatt gtggaagaa 
      gtggcgcca ggaggtgca gccgcatct actgagctc 
      aagagtaag gatgaaagg aatgaaagt cctgcggct 
      gaccgaaat ggcagatat ctcaagtct ggtcatgcc 
      aggacgcat gccattgtt ataaatctt gacgataaa 
      agccgattc acggaagaa gtgacggtg taa
    mRNA sequence
    • >TC010857 RA
      ctctgccgg ccttgcaat cgctcggac gcgccacct 
      ccaacccca aacgcgtcc aaatcacgc agcgatttg 
      cggacttgt cccaaaaat tttcccgag gcaggacgg 
      acctcgctc gagccttga tggcccctg taaaatagt 
      ttgagggag ttttcttga cttttgcct gtgtcgatg 
      ttaaatctc taattgtct aatatgtaa ataatggtt 
      gttccgatt agacgtagc atgaagtta tttttgttg 
      ttgttgctg ttgtttgag ttgggagcg caaaatgtt 
      cctttggac aatataagg cacagtcgg tcgcggacg 
      cgcttgctg aggggaaga cggcatggg gatgatgtc 
      cgggaagat ttcacgtct gataatgtt ctcgaggtt 
      ccttcggcg acggaaagc ttgtttttt ggtgatgat 
      tccgggaga aatcggact agtctgtct gtggaggaa 
      cctccgacg agcaaatcg agcgcgagg aaggtgacg 
      gtaggttcg aatttcaac ctccatgga aacagcagt 
      tggcagcat gtggataag agcatgttg tacatgggt 
      gagcgggcg cttaatcaa atcgataac aaaaagaaa 
      aataagaca gtgagtggt aaggagaag gcgagcccc 
      ggagagtca ccgataaac gtgcatcag ggttttgac 
      gaagatgtg gaaatatca tcggaaaag gcagccacc 
      gaaagtgct cgtagcggc cacgtagac atcgttact 
      cgtttttta cgcatcgtg gagtcccag catttgttg 
      ggagagaac tgtacggcc ggcacggac ctgaatttg 
      ggcgaagga gttgtagat cgttatgca caagaaaga 
      tttcgggtg gaggcagac gtggccgta aatagggct 
      aatatgttg actcggctg tggaaatac gccgatcct 
      gatgttgtt atgtcggag tatttattg cacgcgtcc 
      gttttttcc atggttgaa ttcgataat gacatcttt 
      gctgcggga aactgctat gaccagtat cagtacaaa 
      gactattgg ctattctgc ccgtatgct tatcggctg 
      cccgaaggg acaatcctc gtgaaagac ttggccgtt 
      gagtacaaa tatctcagc aacacatcg gaatggttc 
      tacatcgcc cgaaaaaat gccgagaac gtcatcaga 
      aactacaat caattcagt aaaggttac aatacttac 
      acatacaat gaaagtgct cacacggag cgagttccc 
      gacgagatt ttgtcagtg cgttacgaa gatggaaaa 
      tggtcaaag ccttactac gactgtggt ggcggaaac 
      atctggatg ctcacttac actgttccg ttttttggc 
      tattcgaac aatacgtac tacttcaag ggaactagt 
      ggaattgat atagatctg cggcgggtt gatatcgac 
      caatgccct ttgccgaag ggcagcaca cagctcaac 
      attttcgcg gcttcagac aaatgcaaa aagcgcact 
      acggaatgt attccgctg tcaggactg ggcttcagg 
      cggggtagt tacaagtgt atatgcaaa cgcggcttt 
      ttctttccg gacaccaaa gcggacagg cgctactat 
      aatggaacc gtgatcgaa gaggaatat gaaaaactg 
      atgttgggc gaaaccagt cagtacgcg aaagctgga 
      attttcgaa tgtctctca tgtcctgaa ggatgtgaa 
      acctgtgag gacgaccga ccatgtgtg gtttctctc 
      aactgggta atgcgaacg gtcattctc gtcctttcc 
      tgtgtcatc atctgctgt ttacccatc gttgtcgtc 
      tttacctgg aaatacggg aatgtgaag gtggtacgt 
      gccgccagt cctgttcta ttatgcttc ataactttg 
      ggagctttc ctcatatat tgtacgatg attgtgatg 
      taccctcgt cctaatatg tacacgtgc atagcacga 
      gtgtggctt agagaaatt ggcttttca ttaacctac 
      ggagctttg atgctgaag acgtggcga atttccgtc 
      atcttccgc gttcgttca gctaaggcc gtcaagatt 
      acagacatg aatttgcta aagcggctt ggtgttata 
      gttgccatt tttactgtt tttctggct attcgaact 
      cttgttgct ccgccagtc gtcatcgtc ggccgaaca 
      gctgatgac cttaaggcc tatttgtgc cgcactgat 
      tggtgggac cactcattc acgacactc gaagtcatg 
      tttctggtt tggggaata cgactgtgc atcgtcgta 
      cggaaagct ccatccgag tttaatgaa agtcgcttt 
      atttccatg gccatttat aatgaattc ctactatct 
      gtctttctc aatatatca atgttgttc ctgcaaaaa 
      cctgctaac ccagacctg ctctacatt attttcttc 
      tgtcatacg cagctcacc gtaacatta cttctgtgc 
      ctcatattc ggaagcaag gcgtatatg gtgtttaag 
      caccatgac caaagcgac gacagctct ggtatgatg 
      tcaaagacg ccgacttca aaatttcta gggaaagcg 
      cgtgcaggg aattcgcaa tatacaagt acgaactcg 
      tcagttggc ggtttagag tattcaaaa tattcggag 
      caagatgtc caggaagag ttcctgcgt ttgtacacg 
      cagctggag ttgttgaaa gaaaaaaat atgcggatt 
      gggaatagg catttggcg tctaagata accgcgatg 
      caggatgct gcgaggagt catgatcat gtacaatcg 
      gaaaatcca tccagaccc cttgggaaa ttaaataat 
      acactggat gtagttccg gagaaatcg ggcaacgac 
      gtaagtaca gaggacgac gcacccgtt aaaattaaa 
      cgtcaaagt gtttcattc gcgtcggaa actgaagcc 
      agtggcgat cctgcagtt aaagataat aatagttgt 
      agtaagatt gtggaagaa gtggcgcca ggaggtgca 
      gccgcatct actgagctc aagagtaag gatgaaagg 
      aatgaaagt cctgcggct gaccgaaat ggcagatat 
      ctcaagtct ggtcatgcc aggacgcat gccattgtt 
      ataaatctt gacgataaa agccgattc acggaagaa 
      gtgacggtg taatattat cattaagta cctgataat 
      taacaatac ttattaatt ttaagtcac gacagttag 
      agtagctaa catgagccc cgttagctg ctataaccg 
      actattatg aatccgctt ctatcggtt ttatgctta 
      gatttggtt gtcctatgt ggatgtgtt ggccattcc 
      gctgagctt agacatatc tttctacaa taattgttg 
      taattataa aaattttta tacccaagt ctttccgga 
      ggaaatcgt catttctag ttaagattt atatgtaaa 
      tactataaa aatatgtaa tactattaa catcataaa 
      tatatgtat atagccgcc gatgattac acagcaatt 
      actcctctc ccaatgttg taaaataaa aataaacgt 
      aagctccca gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY