Status
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Gene information
General information
TC033757
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008910
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11674312..11683197
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033757
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08762

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033757 RA
      MKVFNQSVI FFAILYVST SISSTIIIV EDAKACFRR 
      VLAGKRVTR ALVKRALFC ERVEDCQRE CADEKRFPC 
      EGFNYRLDP SGRGKGECE LLEIPLSHL DIGRDLYPD 
      PDYDYYERD RNAASANCK GGYYEQYGG NDGYYNRRH 
      DQRPFDWWE EDRNRYGNR RPRPDFGYN RPDYGYDGY 
      RPNGNRRGD FAYHHSHHS DPHNYHFEH YDHGLAPYD 
      YKSYEPYLP PHRRPPYDE DRFYNKHYG NRDREKNYW 
      GIAKPTWGT YGGSYGTSY NPHEFDRRN YYLPPQIGG 
      SKDWGQYGG SYGNGGLNL QYNGYGHSQ SFDFWGLNK 
      YEEKFHNYG ELPPNKPPS GGSYPPLKP PSNEGGNYL 
      PVLPPKRPT SGSYLPENP PSYLPVLPP KSPNEGSYL 
      PEKPSHGGS YLPEIPSKP PNGGSYLPE LPGRYPINT 
      GSYLPLPGK HDNDFYNSI LPAEPRYPY RYDFLKDEC 
      SLRTAAGFR LQKGIVKKF YAVPNIYEC ELLCFKEKE 
      FPCASYAFR YTVSLTSPT DNCYLSNRN YKELDYYTD 
      LEPDRDFDV YTMNNMKKC DQPVIRGKE HSECFWRVR 
      SAQRLDHKV VRDSLSVKS IVDCQLECL KSIGFTCRA 
      FSYRYGSPV IGGPVDNCL LTDWPYYEM DPRIHFVPE 
      PGFEIYERG SFGHGCEPH HFGIRGRPW KNPGMRLDQ 
      LCYSGYGSP SKLLPQACR KEIQVATEE ECKAECSKY 
      RDKTLFHCM SLSFSTRSS KWEPNCRLS DILQRDLLP 
      NVDYIPDPD SWLFAWDNY NPECTATAH NPSHNHIGH 
      KEDRNDFDD MSNAIGTWR VYSVSGWPC RRGSRCREN 
      REAGFWFCE LEGGDSNTW DYCCRPDHQ CGFSHGFEY 
      QWCYVGPSR TQWRKCSDR YYPYVHNLI DRFDRPPAS 
      APWTPNNYL PDTRPPPYL PGGRPDRPP APPTRPPPR 
      PTLDEYEMQ FESQFLDPP KPGGFGQPR HWPLSYLHK 
      EMPPNSTDS DSRLMKAVQ GRDSNPKFA AIQNLIDII 
      KSNDLKNVQ YQITNESNK HDDILFVKI PLPTNFTQE 
      TRKSEKMVN ATSVISGPI DLGNGGKNT TKRSQKALP 
      VQERPVPVY RRGYITRTN VTNHRPRVS RTL
    CDS sequence
    • >TC033757 RA
      atgaaagtg ttcaaccag tctgtgata ttttttgct 
      atactttat gtttccaca tccatcagc agcaccatt 
      atcatcgtc gaagacgcc aaagcatgc ttcaggaga 
      gtattggcc ggaaaacgc gtaactcgt gcactcgtt 
      aaacgcgcg ttattttgc gaaagagtc gaagattgt 
      caacgcgaa tgtgcagat gaaaaacgg tttccttgc 
      gaaggattt aattacaga ttggatccg tcaggccgt 
      ggaaaaggc gaatgcgag ctcctcgaa atcccttta 
      tcccattta gatatcggg agagatcta taccccgac 
      ccggattac gattactac gaacgagac cgaaacgcc 
      gctagtgca aattgcaaa ggcggttac tacgaacaa 
      tacggtggt aacgacggt tactataac cgacgtcac 
      gaccagaga ccgttcgat tggtgggaa gaagacaga 
      aaccggtac ggtaacaga agaccacgc cccgatttc 
      ggttacaac cggcctgat tacggctac gacggctac 
      aggccgaat ggtaacaga cggggagac tttgcctat 
      catcattca caccattcc gacccacac aattatcat 
      tttgagcac tatgatcat ggtcttgca ccatatgat 
      tacaaatca tacgagcca tatttacct cctcacaga 
      aggccacca tatgacgaa gacaggttt tacaacaaa 
      cactacgga aaccgagac agagagaaa aactactgg 
      gggatcgct aaaccgaca tggggcaca tatggaggc 
      tcgtacgga acgagttat aacccccac gagttcgac 
      cgaagaaat tattacttg cccccgcaa atcggcggt 
      tcgaaagac tggggtcaa tatgggggt agttatggg 
      aatggcggt ctaaactta caatacaac ggttacgga 
      cacagccaa agtttcgac ttctggggg ctaaacaaa 
      tacgaggag aaattccac aattatgga gaattgccc 
      ccgaataaa cctccaagt gggggaagc tatccccct 
      ctgaaacct ccaagtaat gaaggaggt aactacttg 
      ccggtatta ccaccgaaa cgaccaacc agcggaagt 
      taccttcca gaaaacccg ccaagttat ttacccgtg 
      ttaccacct aaatctccc aatgaggga agttacttg 
      ccggaaaaa ccctctcat gggggcagt tacttgccg 
      gaaataccc tcaaaaccc ccaaatggc ggtagttat 
      ttgccggaa ttgcccgga agatatccg ataaatacc 
      ggaagttat ttgccgttg ccgggtaaa cacgataac 
      gatttttac aattcaatt cttccggct gaaccgagg 
      tatccgtat cgctacgat tttctcaag gacgagtgc 
      tctttgaga actgcggca ggttttagg ttgcaaaaa 
      ggcatagtt aaaaagttt tatgctgtg ccgaatatt 
      tacgaatgc gaacttttg tgtttcaaa gagaaggaa 
      tttccttgt gcatcgtat gccttcaga tacacggtt 
      tctttgact tcgccaacg gacaattgt tatttgagt 
      aatcgcaat tataaggaa ttagactat tacacggac 
      ttggaaccc gacagagac tttgatgtt tacactatg 
      aacaatatg aagaaatgt gaccaacct gtaattcgt 
      ggcaaagaa catagtgag tgcttctgg cgcgtccga 
      agcgcccaa agactggac cacaaagtc gtccgcgat 
      tccctatca gtgaaatca atcgtagac tgccagctc 
      gaatgttta aaatcaatt ggtttcaca tgtcgagct 
      ttcagctac agatacgga tctccggta atagggggc 
      ccagtcgac aactgtctt ttaaccgac tggccatat 
      tacgaaatg gacccccga attcacttc gtacccgaa 
      cccggcttc gaaatttac gaaagaggg agttttgga 
      catggatgc gaaccgcac catttcggg attagggga 
      cgcccttgg aaaaaccca gggatgcga ctagaccaa 
      ctgtgctat agtggatac ggttctccc tcaaaactg 
      ttgccacaa gcttgcaga aaagaaatt caagttgcg 
      actgaggag gaatgcaag gccgaatgt tcgaaatat 
      agggacaag acgctattc cactgcatg tcactaagt 
      ttcagcacc agaagttca aagtgggaa ccaaactgt 
      cgattatcg gatatttta caaagggat ttattgccc 
      aatgttgac tatattccc gatcccgac tcttggctc 
      tttgcttgg gataattat aatccagag tgtacagca 
      actgcccac aaccccagt cacaatcac atagggcac 
      aaggaagac cgcaacgat ttcgatgac atgtccaac 
      gctattgga acctggaga gtctacagt gtcagtgga 
      tggccttgc cgcagagga agccgttgt cgggaaaac 
      agggaagca ggattttgg ttctgcgaa ttagaaggg 
      ggtgattca aacacttgg gattactgc tgccgacct 
      gaccatcag tgtgggttc tcgcacggt tttgaatat 
      caatggtgt tacgtgggc ccctcgcgc acgcagtgg 
      cgcaaatgc agcgaccgc tactacccc tacgtgcac 
      aacctcatc gaccgcttt gaccgcccc ccggcctcg 
      gccccctgg acccccaat aattacctc ccggacacg 
      cgtccgccc ccgtacctg cctggaggc cgccccgat 
      cggcccccg gcgccgcct acacggcca cccccacgt 
      ccgaccctt gacgagtac gaaatgcag ttcgagagc 
      cagttcttg gatccccct aaacctggg ggctttggg 
      caaccgcga cactggccc ttgtcttat ttgcacaag 
      gagatgcct cccaatagc accgactcg gactcgagg 
      ctcatgaaa gccgtccaa ggccgcgac agcaacccc 
      aaattcgcg gccattcag aatttgatc gatatcata 
      aaatcgaat gatctcaag aatgttcag taccagata 
      accaacgaa tcgaataaa catgacgat attttgttt 
      gtgaagatt ccactgccg acgaatttt acgcaagag 
      acgcggaaa agcgagaaa atggtgaat gcgacgagt 
      gttatttcc ggtccgatt gatttggga aatggaggg 
      aaaaatacc acgaaaaga tctcaaaag gcactgccg 
      gtacaagag aggcctgtg cccgtttac agaagaggg 
      tacataacc aggactaac gttactaat catagaccg 
      agagttagt agaacactg taa
    mRNA sequence
    • >TC033757 RA
      acaaaatcc cagttgtag gtgttcgtt gcttaaagt 
      taacgtttt cggtttgtt tttctaaac caacaccga 
      cgccggttt tgttattgt aatatattg agagaagaa 
      agggaattt ttcgcaatt cctgtttgt atgttaatt 
      tcttatgta tatcgcacc ttgggaaca agaaggttc 
      tattgagac atggtcaaa ttaaaatta cattcttaa 
      ttgtcttaa taataaatc ttaaagtta tgttaatta 
      aatagtgtt aaataatac cgaaagctt gcataatga 
      cacaactag cagcgtcgg ggttttagt tgtgatcga 
      cgggttttt actagaaaa tgaaagtgt tcaaccagt 
      ctgtgatat tttttgcta tactttatg tttccacat 
      ccatcagca gcaccatta tcatcgtcg aagacgcca 
      aagcatgct tcaggagag tattggccg gaaaacgcg 
      taactcgtg cactcgtta aacgcgcgt tattttgcg 
      aaagagtcg aagattgtc aacgcgaat gtgcagatg 
      aaaaacggt ttccttgcg aaggattta attacagat 
      tggatccgt caggccgtg gaaaaggcg aatgcgagc 
      tcctcgaaa tccctttat cccatttag atatcggga 
      gagatctat accccgacc cggattacg attactacg 
      aacgagacc gaaacgccg ctagtgcaa attgcaaag 
      gcggttact acgaacaat acggtggta acgacggtt 
      actataacc gacgtcacg accagagac cgttcgatt 
      ggtgggaag aagacagaa accggtacg gtaacagaa 
      gaccacgcc ccgatttcg gttacaacc ggcctgatt 
      acggctacg acggctaca ggccgaatg gtaacagac 
      ggggagact ttgcctatc atcattcac accattccg 
      acccacaca attatcatt ttgagcact atgatcatg 
      gtcttgcac catatgatt acaaatcat acgagccat 
      atttacctc ctcacagaa ggccaccat atgacgaag 
      acaggtttt acaacaaac actacggaa accgagaca 
      gagagaaaa actactggg ggatcgcta aaccgacat 
      ggggcacat atggaggct cgtacggaa cgagttata 
      acccccacg agttcgacc gaagaaatt attacttgc 
      ccccgcaaa tcggcggtt cgaaagact ggggtcaat 
      atgggggta gttatggga atggcggtc taaacttac 
      aatacaacg gttacggac acagccaaa gtttcgact 
      tctgggggc taaacaaat acgaggaga aattccaca 
      attatggag aattgcccc cgaataaac ctccaagtg 
      ggggaagct atccccctc tgaaacctc caagtaatg 
      aaggaggta actacttgc cggtattac caccgaaac 
      gaccaacca gcggaagtt accttccag aaaacccgc 
      caagttatt tacccgtgt taccaccta aatctccca 
      atgagggaa gttacttgc cggaaaaac cctctcatg 
      ggggcagtt acttgccgg aaataccct caaaacccc 
      caaatggcg gtagttatt tgccggaat tgcccggaa 
      gatatccga taaataccg gaagttatt tgccgttgc 
      cgggtaaac acgataacg atttttaca attcaattc 
      ttccggctg aaccgaggt atccgtatc gctacgatt 
      ttctcaagg acgagtgct ctttgagaa ctgcggcag 
      gttttaggt tgcaaaaag gcatagtta aaaagtttt 
      atgctgtgc cgaatattt acgaatgcg aacttttgt 
      gtttcaaag agaaggaat ttccttgtg catcgtatg 
      ccttcagat acacggttt ctttgactt cgccaacgg 
      acaattgtt atttgagta atcgcaatt ataaggaat 
      tagactatt acacggact tggaacccg acagagact 
      ttgatgttt acactatga acaatatga agaaatgtg 
      accaacctg taattcgtg gcaaagaac atagtgagt 
      gcttctggc gcgtccgaa gcgcccaaa gactggacc 
      acaaagtcg tccgcgatt ccctatcag tgaaatcaa 
      tcgtagact gccagctcg aatgtttaa aatcaattg 
      gtttcacat gtcgagctt tcagctaca gatacggat 
      ctccggtaa tagggggcc cagtcgaca actgtcttt 
      taaccgact ggccatatt acgaaatgg acccccgaa 
      ttcacttcg tacccgaac ccggcttcg aaatttacg 
      aaagaggga gttttggac atggatgcg aaccgcacc 
      atttcggga ttaggggac gcccttgga aaaacccag 
      ggatgcgac tagaccaac tgtgctata gtggatacg 
      gttctccct caaaactgt tgccacaag cttgcagaa 
      aagaaattc aagttgcga ctgaggagg aatgcaagg 
      ccgaatgtt cgaaatata gggacaaga cgctattcc 
      actgcatgt cactaagtt tcagcacca gaagttcaa 
      agtgggaac caaactgtc gattatcgg atattttac 
      aaagggatt tattgccca atgttgact atattcccg 
      atcccgact cttggctct ttgcttggg ataattata 
      atccagagt gtacagcaa ctgcccaca accccagtc 
      acaatcaca tagggcaca aggaagacc gcaacgatt 
      tcgatgaca tgtccaacg ctattggaa cctggagag 
      tctacagtg tcagtggat ggccttgcc gcagaggaa 
      gccgttgtc gggaaaaca gggaagcag gattttggt 
      tctgcgaat tagaagggg gtgattcaa acacttggg 
      attactgct gccgacctg accatcagt gtgggttct 
      cgcacggtt ttgaatatc aatggtgtt acgtgggcc 
      cctcgcgca cgcagtggc gcaaatgca gcgaccgct 
      actacccct acgtgcaca acctcatcg accgctttg 
      accgccccc cggcctcgg ccccctgga cccccaata 
      attacctcc cggacacgc gtccgcccc cgtacctgc 
      ctggaggcc gccccgatc ggcccccgg cgccgccta 
      cacggccac ccccacgtc cgacccttg acgagtacg 
      aaatgcagt tcgagagcc agttcttgg atcccccta 
      aacctgggg gctttgggc aaccgcgac actggccct 
      tgtcttatt tgcacaagg agatgcctc ccaatagca 
      ccgactcgg actcgaggc tcatgaaag ccgtccaag 
      gccgcgaca gcaacccca aattcgcgg ccattcaga 
      atttgatcg atatcataa aatcgaatg atctcaaga 
      atgttcagt accagataa ccaacgaat cgaataaac 
      atgacgata ttttgtttg tgaagattc cactgccga 
      cgaatttta cgcaagaga cgcggaaaa gcgagaaaa 
      tggtgaatg cgacgagtg ttatttccg gtccgattg 
      atttgggaa atggaggga aaaatacca cgaaaagat 
      ctcaaaagg cactgccgg tacaagaga ggcctgtgc 
      ccgtttaca gaagagggt acataacca ggactaacg 
      ttactaatc atagaccga gagttagta gaacactgt 
      aattgtagg atattttgt aaatgcaat tttgtatca 
      ataaagaca tttttcgta a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY