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Gene information
General information
TC033755
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009924 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11770492..11774661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033755
    iBeetle screen iB_08760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033755 RA
      MSKNKFDVK SVLNNKIEV KSKFDAEFR RFSVERDPQ 
ARFEEFRAL LERLHRLQD IAFLVSYID PSDQDLLPI 
NNDDNLRRA LANAKPLLR VIIQRKGDS LELNGYGTI 
KPRNLISSI LGGTPGRTK TLEISNPHD FRQVSSIID 
VDIVPETCR RVRLLKHGS DKPLGFYIR DGTSVRVTQ 
SGLEKIPGI FISRLVPGG LAESTGLLA VNDEVLEVN 
GIEVAGKTL DQVTDMMVA NSSNLIITV KPANQRTVA 
PVRRGSFSR NSQLSSGSQ QSHTTGGSD PDDKYDQDE 
IVDLTGATN LDESSEDGI LHL
    CDS sequence
    • >TC033755 RA
      atgtccaaa aacaagttt gatgttaaa agtgtgctc 
aataacaaa atagaagtt aaaagcaaa ttcgacgcc 
gagtttcga cggttctcg gttgagaga gaccctcaa 
gctcgattt gaagaattc cgggctttg ttagagcgc 
ttgcacaga ctgcaggac atagcattt ttggtttcg 
tatattgac ccgagcgat caggatttg ctgccaatc 
aacaatgat gataacctt cgaagggcc ttagcaaat 
gcaaaaccc ctcctgagg gtcataatc caaagaaaa 
ggtgatagt cttgaactg aatggatac gggactatc 
aagccacga aatttaatt tctagcata ttaggcggc 
acccccgga cgaacaaag accttggaa atttccaac 
ccccatgat tttcgccaa gtttcatcc atcatagat 
gtagacatt gtacctgag acttgcaga cgcgtgaga 
ctgctgaaa cacggctca gataaaccg ctgggattt 
tacatcagg gatggcaca agtgtgcga gtcacccaa 
agtggtttg gagaaaatt cccggaatt ttcatctcg 
cgtctggtc cccggcggt ttggcagaa tccaccggt 
cttttggcg gtgaacgac gaagtcctc gaagtgaac 
ggaatcgaa gtggcgggg aaaaccctc gaccaggtc 
accgacatg atggtggcc aacagttca aacctgata 
attacagtc aagccggca aatcagaga actgtagct 
ccagtccgt cgcggcagt ttcagccga aactcgcaa 
ctttcaagc gggtcacaa cagtcacac accaccggg 
ggctccgac cctgacgat aagtacgat caggacgaa 
atcgtcgat cttacgggg gctaccaac ctcgatgag 
agctcagaa gatggtatt ctacacctg tag
    mRNA sequence
    • >TC033755 RA
      ctgatgact aaattcacc attacactg tctgggttg 
caagaggtt cttccaatg taaataaat tggaatttg 
atccgtttg ttaagtttt taagttact tttgttctt 
tttttgttg taagaatag ttgaatgtg tcgttagtg 
ataagaaaa gtgccgtaa cacgatttt tttcgatgc 
ccgaaaatg tccaaaaac aagtttgat gttaaaagt 
gtgctcaat aacaaaata gaagttaaa agcaaattc 
gacgccgag tttcgacgg ttctcggtt gagagagac 
cctcaagct cgatttgaa gaattccgg gctttgtta 
gagcgcttg cacagactg caggacata gcatttttg 
gtttcgtat attgacccg agcgatcag gatttgctg 
ccaatcaac aatgatgat aaccttcga agggcctta 
gcaaatgca aaacccctc ctgagggtc ataatccaa 
agaaaaggt gatagtctt gaactgaat ggatacggg 
actatcaag ccacgaaat ttaatttct agcatatta 
ggcggcacc cccggacga acaaagacc ttggaaatt 
tccaacccc catgatttt cgccaagtt tcatccatc 
atagatgta gacattgta cctgagact tgcagacgc 
gtgagactg ctgaaacac ggctcagat aaaccgctg 
ggattttac atcagggat ggcacaagt gtgcgagtc 
acccaaagt ggtttggag aaaattccc ggaattttc 
atctcgcgt ctggtcccc ggcggtttg gcagaatcc 
accggtctt ttggcggtg aacgacgaa gtcctcgaa 
gtgaacgga atcgaagtg gcggggaaa accctcgac 
caggtcacc gacatgatg gtggccaac agttcaaac 
ctgataatt acagtcaag ccggcaaat cagagaact 
gtagctcca gtccgtcgc ggcagtttc agccgaaac 
tcgcaactt tcaagcggg tcacaacag tcacacacc 
accgggggc tccgaccct gacgataag tacgatcag 
gacgaaatc gtcgatctt acgggggct accaacctc 
gatgagagc tcagaagat ggtattcta cacctgtag 
gtgctctgt agtatttat tgcttccgt tggctgtaa 
aagactgtg gattttttt acaggtaac tagcgtttg 
gtattattt taacggtta ctgacttaa ttcaaaagc 
ggtggtcac gactggcga gtcatatct gcatttatc 
gtatttttt aattctatt caagtactt aactggaac 
aagataaga cttaagaaa cattccctt tttattcca 
aatatttta ttccattat catgtgttc tatcgagta 
attccattt atatcatgt tttattgat ttaattatt 
ttaaggaat attattatc gttattcag tattttagt 
ttagtccat tgtgtctca aacggaacc aagttccgt 
cccttcgtc gaatttatg taatggtgg ctacgacaa 
gtattacat ttttatgat ttttcgtgt tattgtgtc 
aaaatcact tttacaaca gctattttt taaacagtg 
taaataata ttattgtaa gtaccttta agatgtgaa 
catactgtt tgtaggtta tacatttta catttttat 
aaacataat catgaaaga ggccttttt tctcatcaa 
tcactatag ggaaaaatt ccgtccttg tattgcctt 
gaatcgagc ttgtgactt ttttaatgt aatgatatt 
gttaaaggt acattttat gcaaatgtt atagttgca 
tcacatatt atgtactta tgaaaaagt tattcgaaa 
tatatatct caagttaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY