Status
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Gene information
General information
TC033755
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009924
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11770492..11774661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033755
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033755 RA
      MSKNKFDVK SVLNNKIEV KSKFDAEFR RFSVERDPQ 
      ARFEEFRAL LERLHRLQD IAFLVSYID PSDQDLLPI 
      NNDDNLRRA LANAKPLLR VIIQRKGDS LELNGYGTI 
      KPRNLISSI LGGTPGRTK TLEISNPHD FRQVSSIID 
      VDIVPETCR RVRLLKHGS DKPLGFYIR DGTSVRVTQ 
      SGLEKIPGI FISRLVPGG LAESTGLLA VNDEVLEVN 
      GIEVAGKTL DQVTDMMVA NSSNLIITV KPANQRTVA 
      PVRRGSFSR NSQLSSGSQ QSHTTGGSD PDDKYDQDE 
      IVDLTGATN LDESSEDGI LHL
    CDS sequence
    • >TC033755 RA
      atgtccaaa aacaagttt gatgttaaa agtgtgctc 
      aataacaaa atagaagtt aaaagcaaa ttcgacgcc 
      gagtttcga cggttctcg gttgagaga gaccctcaa 
      gctcgattt gaagaattc cgggctttg ttagagcgc 
      ttgcacaga ctgcaggac atagcattt ttggtttcg 
      tatattgac ccgagcgat caggatttg ctgccaatc 
      aacaatgat gataacctt cgaagggcc ttagcaaat 
      gcaaaaccc ctcctgagg gtcataatc caaagaaaa 
      ggtgatagt cttgaactg aatggatac gggactatc 
      aagccacga aatttaatt tctagcata ttaggcggc 
      acccccgga cgaacaaag accttggaa atttccaac 
      ccccatgat tttcgccaa gtttcatcc atcatagat 
      gtagacatt gtacctgag acttgcaga cgcgtgaga 
      ctgctgaaa cacggctca gataaaccg ctgggattt 
      tacatcagg gatggcaca agtgtgcga gtcacccaa 
      agtggtttg gagaaaatt cccggaatt ttcatctcg 
      cgtctggtc cccggcggt ttggcagaa tccaccggt 
      cttttggcg gtgaacgac gaagtcctc gaagtgaac 
      ggaatcgaa gtggcgggg aaaaccctc gaccaggtc 
      accgacatg atggtggcc aacagttca aacctgata 
      attacagtc aagccggca aatcagaga actgtagct 
      ccagtccgt cgcggcagt ttcagccga aactcgcaa 
      ctttcaagc gggtcacaa cagtcacac accaccggg 
      ggctccgac cctgacgat aagtacgat caggacgaa 
      atcgtcgat cttacgggg gctaccaac ctcgatgag 
      agctcagaa gatggtatt ctacacctg tag
    mRNA sequence
    • >TC033755 RA
      ctgatgact aaattcacc attacactg tctgggttg 
      caagaggtt cttccaatg taaataaat tggaatttg 
      atccgtttg ttaagtttt taagttact tttgttctt 
      tttttgttg taagaatag ttgaatgtg tcgttagtg 
      ataagaaaa gtgccgtaa cacgatttt tttcgatgc 
      ccgaaaatg tccaaaaac aagtttgat gttaaaagt 
      gtgctcaat aacaaaata gaagttaaa agcaaattc 
      gacgccgag tttcgacgg ttctcggtt gagagagac 
      cctcaagct cgatttgaa gaattccgg gctttgtta 
      gagcgcttg cacagactg caggacata gcatttttg 
      gtttcgtat attgacccg agcgatcag gatttgctg 
      ccaatcaac aatgatgat aaccttcga agggcctta 
      gcaaatgca aaacccctc ctgagggtc ataatccaa 
      agaaaaggt gatagtctt gaactgaat ggatacggg 
      actatcaag ccacgaaat ttaatttct agcatatta 
      ggcggcacc cccggacga acaaagacc ttggaaatt 
      tccaacccc catgatttt cgccaagtt tcatccatc 
      atagatgta gacattgta cctgagact tgcagacgc 
      gtgagactg ctgaaacac ggctcagat aaaccgctg 
      ggattttac atcagggat ggcacaagt gtgcgagtc 
      acccaaagt ggtttggag aaaattccc ggaattttc 
      atctcgcgt ctggtcccc ggcggtttg gcagaatcc 
      accggtctt ttggcggtg aacgacgaa gtcctcgaa 
      gtgaacgga atcgaagtg gcggggaaa accctcgac 
      caggtcacc gacatgatg gtggccaac agttcaaac 
      ctgataatt acagtcaag ccggcaaat cagagaact 
      gtagctcca gtccgtcgc ggcagtttc agccgaaac 
      tcgcaactt tcaagcggg tcacaacag tcacacacc 
      accgggggc tccgaccct gacgataag tacgatcag 
      gacgaaatc gtcgatctt acgggggct accaacctc 
      gatgagagc tcagaagat ggtattcta cacctgtag 
      gtgctctgt agtatttat tgcttccgt tggctgtaa 
      aagactgtg gattttttt acaggtaac tagcgtttg 
      gtattattt taacggtta ctgacttaa ttcaaaagc 
      ggtggtcac gactggcga gtcatatct gcatttatc 
      gtatttttt aattctatt caagtactt aactggaac 
      aagataaga cttaagaaa cattccctt tttattcca 
      aatatttta ttccattat catgtgttc tatcgagta 
      attccattt atatcatgt tttattgat ttaattatt 
      ttaaggaat attattatc gttattcag tattttagt 
      ttagtccat tgtgtctca aacggaacc aagttccgt 
      cccttcgtc gaatttatg taatggtgg ctacgacaa 
      gtattacat ttttatgat ttttcgtgt tattgtgtc 
      aaaatcact tttacaaca gctattttt taaacagtg 
      taaataata ttattgtaa gtaccttta agatgtgaa 
      catactgtt tgtaggtta tacatttta catttttat 
      aaacataat catgaaaga ggccttttt tctcatcaa 
      tcactatag ggaaaaatt ccgtccttg tattgcctt 
      gaatcgagc ttgtgactt ttttaatgt aatgatatt 
      gttaaaggt acattttat gcaaatgtt atagttgca 
      tcacatatt atgtactta tgaaaaagt tattcgaaa 
      tatatatct caagttaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY