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Gene information
General information
TC009872 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12833701..12836225
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009872
    iBeetle screen iB_08752

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009872 RA
      MVSLKNFCA VFVIFVVAF MDEVKALTG PSHGKVVVC 
      YLGTWSVYR PGRGSFTIE HLDPALCTH IVYSFAGLD 
      EKTDSIKSL DPFQDLADD YGKEGYQKL TRLKHRYPH 
      LKVTLAIGG WNEGSLKYS ELAKHANRR SRFVSSVVD 
      FLRKYDFDG LDLDWEFPG KRGGAPEDK LNFLILVKE 
      LKAAFSKYN LLLTAAFGA GKDTIDAAY DVKGLSLYL 
      DFIHMMCYD YHGAWDQKT GANAPLQSS DVLNVEFSI 
      NYMLKLGAP AKKLVLGVP LYGRTFLLP EPYDPESGR 
      KPKLGAVAR NIGFQGPFT RENGFMGYN EICLEVKNK 
      SQNWAKFWD SESKTPYAV SGNKVLTYD DVQSIEEKV 
      KFAIEKDLG GIMVWSIDT DDFHGDCAD VGEDGHFEN 
      FPLMRAINK AIPKSIEDR RNSVQWTGV NTSGAGSSS 
      SLLFLVAMA IALFIKF
    CDS sequence
    • >TC009872 RA
      atggtatca ctaaaaaat ttttgtgcg gtttttgtg 
      attttcgta gtggctttc atggatgaa gttaaggcc 
      cttactggt ccaagccat gggaaagtc gtcgtttgt 
      tatttaggg acgtggtcg gtctataga ccaggcaga 
      ggctctttc accattgag cacctggac cccgcgtta 
      tgcacccac attgtctac tccttcgcc ggcttggac 
      gaaaaaacc gacagtatt aagtcacta gaccccttc 
      caagatttg gccgacgat tatggcaaa gaggggtac 
      cagaaactg acgcggctt aagcaccgg tacccccat 
      ttgaaagtc acgctggcc atcgggggc tggaacgag 
      ggctccctc aaatactca gaactggca aaacacgcg 
      aacaggcga agcagattt gtctcaagc gtcgttgat 
      ttcctcagg aaatacgac tttgatggc cttgacttg 
      gactgggag tttcctgga aaacgcggc ggcgcccct 
      gaagataaa ctcaacttc cttatctta gtcaaagaa 
      ctcaaagcc gcgttcagc aaatacaat ctcctcctg 
      actgcggcc ttcggggcc gggaaagac accatagac 
      gcagcgtac gacgtcaaa ggcctctcg ctttactta 
      gacttcatc cacatgatg tgctacgac taccacggg 
      gcttgggac cagaaaacc ggggccaat gcgcccctg 
      caaagctcc gacgtccta aacgtcgaa ttctcgatc 
      aattacatg ctgaagctc ggagctccc gccaaaaaa 
      ctcgtcctg ggggttccc ctctacggc cgcacgttc 
      ctcctgccg gagccctac gacccggag tccgggcgc 
      aaacctaaa ctgggggct gtcgcccgc aacataggg 
      ttccaaggg ccgttcacg cgagagaac gggtttatg 
      ggttacaat gagatttgc ttggaggtg aagaacaag 
      tcgcaaaac tgggccaag ttctgggac agcgagtcg 
      aaaacgccg tacgcagtg tcgggcaat aaagtcttg 
      acttacgac gacgtgcag tcgatcgaa gagaaagtt 
      aaatttgcg attgagaag gacttgggt gggattatg 
      gtctggtcg attgacacc gatgatttt cacggggat 
      tgtgccgat gttggggag gatggacat ttcgagaac 
      ttccccctg atgagggct attaacaaa gctataccc 
      aagtcgatt gaggacagg aggaattct gttcaatgg 
      acaggggtg aacaccagc ggggctgga agtagcagt 
      agtttgttg tttttggtt gctatggcc attgcgtta 
      tttataaaa ttctaa
    mRNA sequence
    • >TC009872 RA
      ttaagcggg taaccccca actggacac attcattgt 
      atctttctc tgtctcaaa agtaccaaa aacgcgcgc 
      gcgaaagag aaagctgct accttgcca ttataactg 
      taatatata ttgtacaag aaatataaa gttcatctc 
      tcatttgtg caaaagttg tgagaatac caagatggt 
      atcactaaa aaatttttg tgcggtttt tgtgatttt 
      cgtagtggc tttcatgga tgaagttaa ggcccttac 
      tggtccaag ccatgggaa agtcgtcgt ttgttattt 
      agggacgtg gtcggtcta tagaccagg cagaggctc 
      tttcaccat tgagcacct ggaccccgc gttatgcac 
      ccacattgt ctactcctt cgccggctt ggacgaaaa 
      aaccgacag tattaagtc actagaccc cttccaaga 
      tttggccga cgattatgg caaagaggg gtaccagaa 
      actgacgcg gcttaagca ccggtaccc ccatttgaa 
      agtcacgct ggccatcgg gggctggaa cgagggctc 
      cctcaaata ctcagaact ggcaaaaca cgcgaacag 
      gcgaagcag atttgtctc aagcgtcgt tgatttcct 
      caggaaata cgactttga tggccttga cttggactg 
      ggagtttcc tggaaaacg cggcggcgc ccctgaaga 
      taaactcaa cttccttat cttagtcaa agaactcaa 
      agccgcgtt cagcaaata caatctcct cctgactgc 
      ggccttcgg ggccgggaa agacaccat agacgcagc 
      gtacgacgt caaaggcct ctcgcttta cttagactt 
      catccacat gatgtgcta cgactacca cggggcttg 
      ggaccagaa aaccggggc caatgcgcc cctgcaaag 
      ctccgacgt cctaaacgt cgaattctc gatcaatta 
      catgctgaa gctcggagc tcccgccaa aaaactcgt 
      cctgggggt tcccctcta cggccgcac gttcctcct 
      gccggagcc ctacgaccc ggagtccgg gcgcaaacc 
      taaactggg ggctgtcgc ccgcaacat agggttcca 
      agggccgtt cacgcgaga gaacgggtt tatgggtta 
      caatgagat ttgcttgga ggtgaagaa caagtcgca 
      aaactgggc caagttctg ggacagcga gtcgaaaac 
      gccgtacgc agtgtcggg caataaagt cttgactta 
      cgacgacgt gcagtcgat cgaagagaa agttaaatt 
      tgcgattga gaaggactt gggtgggat tatggtctg 
      gtcgattga caccgatga ttttcacgg ggattgtgc 
      cgatgttgg ggaggatgg acatttcga gaacttccc 
      cctgatgag ggctattaa caaagctat acccaagtc 
      gattgagga caggaggaa ttctgttca atggacagg 
      ggtgaacac cagcggggc tggaagtag cagtagttt 
      gttgttttt ggttgctat ggccattgc gttatttat 
      aaaattcta aatttgtga tttttccta gttttaagt 
      ttattatgc agaaaatgt acaactttc agcgacaaa 
      taaagacag atacgtcac cttgttaat attcaggtc 
      agttcttgt tttcaagtc caagaattc gccgattta 
      aaagggaag aaatgaatc acaagtaaa atgagtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY