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Gene information
General information
TC033713
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009849 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9064571..9066565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033713
    iBeetle screen iB_08748

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033713 RA
      MENKTLQIA SDLGIESEE IEIVSINTI CRLCGTHNE 
RLIGIFSDE GVANDLSNK INLYLPVKV AASDELPLQ 
CCWNCASTL LAWHELVVT SVETDRRFR NLQLITEKH 
IGEDAAFDS PVNPPSDDN ADNDGMEPK TIGTSLDDF 
NQTSPNQGS KSAQKSPKK KKKDSITCF PLQETETPL 
ETYITDIEQ ELMDPELVD VPDYKVEIE TDHLLTQED 
DKCNEIKTP EIKPDEEEE RPYFGCQYC PAIFFVQKA 
IADHVREEH GKEYKNCKV HVPKQAPPE AKVVVDGRI 
YYNCKECGK NLHSPYTYV WHIRIHTGE RPFVCDLCG 
KQFRVSQGL VRHLRETHQ GIKKFPCDL CGRMFATRR 
NVEEHRRIH TNERPYVCD LCGKSFKQK ASLFVHKRS 
HKNHFPFKC SYCNQGFRT KPPLLVHIT RHTGEKPYK 
CDICGRCFR IKYELKRHK LIHSDEKPW VCNVCGFSF 
RQKRYLRNH MKSNHTLVA
    CDS sequence
    • >TC033713 RA
      atggaaaat aagacgctg cagatcgct tccgactta 
ggcatcgaa tcagaagaa atcgaaatt gtgagcata 
aatacgatc tgccggttg tgtggcact cacaacgag 
cgtttgatc ggaatattc agcgatgag ggagttgca 
aatgatctc tcgaataaa atcaatctg tatctgcca 
gtgaaagta gcagcttca gacgaactg cctctgcaa 
tgctgctgg aactgtgcc tcaacgttg ctggcctgg 
cacgagttg gttgtaacg agtgttgag acagaccgc 
aggtttagg aacttgcag cttattaca gaaaaacac 
atcggcgaa gatgcggcg tttgatagc cccgtcaac 
ccccccagt gatgacaat gcagacaat gatgggatg 
gagcccaag accatcggc acaagtctg gatgatttc 
aaccaaact tcgccaaac cagggatcg aaaagtgca 
caaaaatcg cccaagaag aagaaaaaa gactcaatc 
acgtgtttt cctctccaa gaaaccgaa acacctctc 
gaaacctac attaccgac atcgaacaa gaactaatg 
gaccccgaa ttggtcgac gttcctgac tacaaagta 
gaaattgaa accgatcat ctcctaaca caggaagac 
gacaaatgt aacgaaata aaaacgcca gagattaag 
ccggacgag gaggaagag cggccctat ttcgggtgc 
caatactgc cctgcgatt ttcttcgtt cagaaagcg 
atagccgac cacgtgaga gaagaacac ggcaaggaa 
tacaagaat tgtaaagtg catgtgcca aagcaagcg 
ccgccggaa gcaaaggtg gttgtagac ggccgtatt 
tactacaac tgcaaggag tgcggaaaa aatctgcac 
tcgccgtac acctacgtc tggcatata agaatacac 
accggtgag cgccccttc gtgtgcgac ttgtgtggg 
aaacaattc cgggtgtcc caaggcttg gtcagacat 
ttgcgtgag actcaccaa ggtattaaa aagttcccc 
tgcgacctg tgtgggcgc atgttcgcc actcgacgt 
aacgtggaa gaacacagg aggattcac acaaacgaa 
agaccgtac gtttgcgac ctctgtggc aagtctttc 
aagcagaag gcgtcactt ttcgtgcac aagaggtcg 
cacaagaac cacttcccg ttcaagtgt agttactgc 
aatcaaggg tttcgaacc aaaccgcct ttactggtg 
catatcacg agacataca ggcgaaaaa ccgtacaag 
tgtgatatt tgcggcagg tgtttccga attaagtac 
gaattgaag cgacataaa cttatacat tcggatgag 
aagccgtgg gtgtgcaat gtgtgtgga ttcagtttt 
agacagaag cggtatttg aggaaccac atgaagtcg 
aaccatacg ctggtggct tag
    mRNA sequence
    • >TC033713 RA
      catcttggt tgttagcgt acgctggtg tactttgtg 
cttctttag tccaattta cacgataaa atttaaccg 
gcctaaaca aaaacattc acttaacct aaaatacgc 
tgggcactc agatggaaa ataagacgc tgcagatcg 
cttccgact taggcatcg aatcagaag aaatcgaaa 
ttgtgagca taaatacga tctgccggt tgtgtggca 
ctcacaacg agcgtttga tcggaatat tcagcgatg 
agggagttg caaatgatc tctcgaata aaatcaatc 
tgtatctgc cagtgaaag tagcagctt cagacgaac 
tgcctctgc aatgctgct ggaactgtg cctcaacgt 
tgctggcct ggcacgagt tggttgtaa cgagtgttg 
agacagacc gcaggttta ggaacttgc agcttatta 
cagaaaaac acatcggcg aagatgcgg cgtttgata 
gccccgtca accccccca gtgatgaca atgcagaca 
atgatggga tggagccca agaccatcg gcacaagtc 
tggatgatt tcaaccaaa cttcgccaa accagggat 
cgaaaagtg cacaaaaat cgcccaaga agaagaaaa 
aagactcaa tcacgtgtt ttcctctcc aagaaaccg 
aaacacctc tcgaaacct acattaccg acatcgaac 
aagaactaa tggaccccg aattggtcg acgttcctg 
actacaaag tagaaattg aaaccgatc atctcctaa 
cacaggaag acgacaaat gtaacgaaa taaaaacgc 
cagagatta agccggacg aggaggaag agcggccct 
atttcgggt gccaatact gccctgcga ttttcttcg 
ttcagaaag cgatagccg accacgtga gagaagaac 
acggcaagg aatacaaga attgtaaag tgcatgtgc 
caaagcaag cgccgccgg aagcaaagg tggttgtag 
acggccgta tttactaca actgcaagg agtgcggaa 
aaaatctgc actcgccgt acacctacg tctggcata 
taagaatac acaccggtg agcgcccct tcgtgtgcg 
acttgtgtg ggaaacaat tccgggtgt cccaaggct 
tggtcagac atttgcgtg agactcacc aaggtatta 
aaaagttcc cctgcgacc tgtgtgggc gcatgttcg 
ccactcgac gtaacgtgg aagaacaca ggaggattc 
acacaaacg aaagaccgt acgtttgcg acctctgtg 
gcaagtctt tcaagcaga aggcgtcac ttttcgtgc 
acaagaggt cgcacaaga accacttcc cgttcaagt 
gtagttact gcaatcaag ggtttcgaa ccaaaccgc 
ctttactgg tgcatatca cgagacata caggcgaaa 
aaccgtaca agtgtgata tttgcggca ggtgtttcc 
gaattaagt acgaattga agcgacata aacttatac 
attcggatg agaagccgt gggtgtgca atgtgtgtg 
gattcagtt ttagacaga agcggtatt tgaggaacc 
acatgaagt cgaaccata cgctggtgg cttagaaaa 
aatcgtaac ggtttgtgg aaataacag ggtgtaacg 
aaaaggtgg ggactaaag gctaccgcc gtttaaaag 
ttagagagc ggtctggtt tttttatac gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY