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Gene information
General information
TC033713
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009849
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9064571..9066565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033713
    iBeetle screen iB_08748

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033713 RA
      MENKTLQIA SDLGIESEE IEIVSINTI CRLCGTHNE 
      RLIGIFSDE GVANDLSNK INLYLPVKV AASDELPLQ 
      CCWNCASTL LAWHELVVT SVETDRRFR NLQLITEKH 
      IGEDAAFDS PVNPPSDDN ADNDGMEPK TIGTSLDDF 
      NQTSPNQGS KSAQKSPKK KKKDSITCF PLQETETPL 
      ETYITDIEQ ELMDPELVD VPDYKVEIE TDHLLTQED 
      DKCNEIKTP EIKPDEEEE RPYFGCQYC PAIFFVQKA 
      IADHVREEH GKEYKNCKV HVPKQAPPE AKVVVDGRI 
      YYNCKECGK NLHSPYTYV WHIRIHTGE RPFVCDLCG 
      KQFRVSQGL VRHLRETHQ GIKKFPCDL CGRMFATRR 
      NVEEHRRIH TNERPYVCD LCGKSFKQK ASLFVHKRS 
      HKNHFPFKC SYCNQGFRT KPPLLVHIT RHTGEKPYK 
      CDICGRCFR IKYELKRHK LIHSDEKPW VCNVCGFSF 
      RQKRYLRNH MKSNHTLVA
    CDS sequence
    • >TC033713 RA
      atggaaaat aagacgctg cagatcgct tccgactta 
      ggcatcgaa tcagaagaa atcgaaatt gtgagcata 
      aatacgatc tgccggttg tgtggcact cacaacgag 
      cgtttgatc ggaatattc agcgatgag ggagttgca 
      aatgatctc tcgaataaa atcaatctg tatctgcca 
      gtgaaagta gcagcttca gacgaactg cctctgcaa 
      tgctgctgg aactgtgcc tcaacgttg ctggcctgg 
      cacgagttg gttgtaacg agtgttgag acagaccgc 
      aggtttagg aacttgcag cttattaca gaaaaacac 
      atcggcgaa gatgcggcg tttgatagc cccgtcaac 
      ccccccagt gatgacaat gcagacaat gatgggatg 
      gagcccaag accatcggc acaagtctg gatgatttc 
      aaccaaact tcgccaaac cagggatcg aaaagtgca 
      caaaaatcg cccaagaag aagaaaaaa gactcaatc 
      acgtgtttt cctctccaa gaaaccgaa acacctctc 
      gaaacctac attaccgac atcgaacaa gaactaatg 
      gaccccgaa ttggtcgac gttcctgac tacaaagta 
      gaaattgaa accgatcat ctcctaaca caggaagac 
      gacaaatgt aacgaaata aaaacgcca gagattaag 
      ccggacgag gaggaagag cggccctat ttcgggtgc 
      caatactgc cctgcgatt ttcttcgtt cagaaagcg 
      atagccgac cacgtgaga gaagaacac ggcaaggaa 
      tacaagaat tgtaaagtg catgtgcca aagcaagcg 
      ccgccggaa gcaaaggtg gttgtagac ggccgtatt 
      tactacaac tgcaaggag tgcggaaaa aatctgcac 
      tcgccgtac acctacgtc tggcatata agaatacac 
      accggtgag cgccccttc gtgtgcgac ttgtgtggg 
      aaacaattc cgggtgtcc caaggcttg gtcagacat 
      ttgcgtgag actcaccaa ggtattaaa aagttcccc 
      tgcgacctg tgtgggcgc atgttcgcc actcgacgt 
      aacgtggaa gaacacagg aggattcac acaaacgaa 
      agaccgtac gtttgcgac ctctgtggc aagtctttc 
      aagcagaag gcgtcactt ttcgtgcac aagaggtcg 
      cacaagaac cacttcccg ttcaagtgt agttactgc 
      aatcaaggg tttcgaacc aaaccgcct ttactggtg 
      catatcacg agacataca ggcgaaaaa ccgtacaag 
      tgtgatatt tgcggcagg tgtttccga attaagtac 
      gaattgaag cgacataaa cttatacat tcggatgag 
      aagccgtgg gtgtgcaat gtgtgtgga ttcagtttt 
      agacagaag cggtatttg aggaaccac atgaagtcg 
      aaccatacg ctggtggct tag
    mRNA sequence
    • >TC033713 RA
      catcttggt tgttagcgt acgctggtg tactttgtg 
      cttctttag tccaattta cacgataaa atttaaccg 
      gcctaaaca aaaacattc acttaacct aaaatacgc 
      tgggcactc agatggaaa ataagacgc tgcagatcg 
      cttccgact taggcatcg aatcagaag aaatcgaaa 
      ttgtgagca taaatacga tctgccggt tgtgtggca 
      ctcacaacg agcgtttga tcggaatat tcagcgatg 
      agggagttg caaatgatc tctcgaata aaatcaatc 
      tgtatctgc cagtgaaag tagcagctt cagacgaac 
      tgcctctgc aatgctgct ggaactgtg cctcaacgt 
      tgctggcct ggcacgagt tggttgtaa cgagtgttg 
      agacagacc gcaggttta ggaacttgc agcttatta 
      cagaaaaac acatcggcg aagatgcgg cgtttgata 
      gccccgtca accccccca gtgatgaca atgcagaca 
      atgatggga tggagccca agaccatcg gcacaagtc 
      tggatgatt tcaaccaaa cttcgccaa accagggat 
      cgaaaagtg cacaaaaat cgcccaaga agaagaaaa 
      aagactcaa tcacgtgtt ttcctctcc aagaaaccg 
      aaacacctc tcgaaacct acattaccg acatcgaac 
      aagaactaa tggaccccg aattggtcg acgttcctg 
      actacaaag tagaaattg aaaccgatc atctcctaa 
      cacaggaag acgacaaat gtaacgaaa taaaaacgc 
      cagagatta agccggacg aggaggaag agcggccct 
      atttcgggt gccaatact gccctgcga ttttcttcg 
      ttcagaaag cgatagccg accacgtga gagaagaac 
      acggcaagg aatacaaga attgtaaag tgcatgtgc 
      caaagcaag cgccgccgg aagcaaagg tggttgtag 
      acggccgta tttactaca actgcaagg agtgcggaa 
      aaaatctgc actcgccgt acacctacg tctggcata 
      taagaatac acaccggtg agcgcccct tcgtgtgcg 
      acttgtgtg ggaaacaat tccgggtgt cccaaggct 
      tggtcagac atttgcgtg agactcacc aaggtatta 
      aaaagttcc cctgcgacc tgtgtgggc gcatgttcg 
      ccactcgac gtaacgtgg aagaacaca ggaggattc 
      acacaaacg aaagaccgt acgtttgcg acctctgtg 
      gcaagtctt tcaagcaga aggcgtcac ttttcgtgc 
      acaagaggt cgcacaaga accacttcc cgttcaagt 
      gtagttact gcaatcaag ggtttcgaa ccaaaccgc 
      ctttactgg tgcatatca cgagacata caggcgaaa 
      aaccgtaca agtgtgata tttgcggca ggtgtttcc 
      gaattaagt acgaattga agcgacata aacttatac 
      attcggatg agaagccgt gggtgtgca atgtgtgtg 
      gattcagtt ttagacaga agcggtatt tgaggaacc 
      acatgaagt cgaaccata cgctggtgg cttagaaaa 
      aatcgtaac ggtttgtgg aaataacag ggtgtaacg 
      aaaaggtgg ggactaaag gctaccgcc gtttaaaag 
      ttagagagc ggtctggtt tttttatac gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY