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Gene information
General information
TC033712
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008999
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8943738..8945725
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033712
    iBeetle screen iB_08744

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033712 RA
      MEKKLAEYR ARKAREEKI NEIKVKTKN 
      FFDGFWKKR PKTPEPSPA EKEALVPPS 
      PSEEYPEDA SSVCSDDES IDCFSYVDL 
      FYYLLWFGL WVVLYVIFI EFQFGTVFL 
      IVSAIVFMY VNTRTGPRK VGEVSAYSV 
      FNKNCESID GTLKAEQFE REIRYGPAV 
      VH
    CDS sequence
    • >TC033712 RA
      atggagaaa aagttggct gaatatcgg gcccgcaaa 
      gcccgagag gagaaaatc aacgaaata aaagtaaaa 
      acgaaaaac tttttcgat ggtttttgg aagaaacgg 
      ccgaagacg cctgaacca agccctgct gagaaagag 
      gctttggtg ccccccagc ccctctgaa gagtacccc 
      gaagatgcg tccagtgtg tgtagcgat gatgagagt 
      attgactgt ttttcatat gtcgatttg ttttattat 
      ttgttgtgg tttggactt tgggtcgtt ttgtatgtg 
      atattcatt gagtttcag ttcggaact gtttttctt 
      attgtttcc gctatcgtt ttcatgtac gtgaacact 
      aggactggg cctaggaaa gtcggggaa gttagtgca 
      tactcggtg ttcaataag aattgcgaa agcattgat 
      gggactttg aaggctgaa cagtttgag agggagata 
      aggtacgga cctgctgtg gttcattaa
    mRNA sequence
    • >TC033712 RA
      tttaaaaat tttgaaaaa aaggtcaat gaacccgct 
      ctaacctca cttatttta ttgtgcgaa aactccagt 
      tccgacata attgccacg tcagtgtta gttaatcac 
      gtaattaaa aaagaaaag tctgcttac cagctgttt 
      tcccactga ttgggccat attttgcac gatttttct 
      aaaaaatct cacaatcgc gctacagct gattacacg 
      tcaaggtgt caataaaca aaacctaaa aatctactt 
      gtaaatgga cccaatgga gaaaaagtt ggctgaata 
      tcgggcccg caaagcccg agaggagaa aatcaacga 
      aataaaagt aaaaacgaa aaacttttt cgatggttt 
      ttggaagaa acggccgaa gacgcctga accaagccc 
      tgctgagaa agaggcttt ggtgccccc cagcccctc 
      tgaagagta ccccgaaga tgcgtccag tgtgtgtag 
      cgatgatga gagtattga ctgtttttc atatgtcga 
      tttgtttta ttatttgtt gtggtttgg actttgggt 
      cgttttgta tgtgatatt cattgagtt tcagttcgg 
      aactgtttt tcttattgt ttccgctat cgttttcat 
      gtacgtgaa cactaggac tgggcctag gaaagtcgg 
      ggaagttag tgcatactc ggtgttcaa taagaattg 
      cgaaagcat tgatgggac tttgaaggc tgaacagtt 
      tgagaggga gataaggta cggacctgc tgtggttca 
      ttaagggcg aaaaaataa caaaaacgt gctgtgata 
      tcattaata ttaaattat tatttataa aagcaagaa 
      taaccccta gtaactgtc tcttgttca tgtatcaga 
      cggggccac attttttat tacttaatt acacaaatt 
      gtactatta tttgcattt attttaata aacgatata 
      aataaatat gagtaacag cattgtaaa aagtatcct 
      aacaaatag tgtcataaa ggtaatcta ctgttttac 
      atttccaat cgaaaatca caattttca taatgagaa 
      aaatattga aggactatt ctcacactc agtgctttt 
      cttaattac attatagta gaaggcaac tttgggtac 
      aagaagtcg ctagctaag gagggaaca caaaattct 
      ttgacttac ggttataaa tctttgttc gagacggtc 
      cctgaaaca atggacgtc taaataaac acataaacg 
      catttctaa tcttaaaga gaaattgga cgaaagtta 
      caacaccgt ttccggctg aaacgtatt cggttgcgg 
      caacagtat cataacacc aacagccat caaaacgtg 
      ccacagact gtgcaaata cttataatt attagacgt 
      ttccaaaaa tataaaaat cgtcaaccc gacacaaac 
      cacgagcat acccagcgg tataaaaac gtacaaata 
      cctcttgtg caaaaacaa ccgcttaac tctcttcca 
      cttcaagaa ccaattaat cgctataat cacaagacc 
      cgaaataac gggcaaaag aaaccccca aattccagt 
      tttatttat agtcgtcgt gaaagacaa gataatagc 
      gccggttat atgacacat agttggcca ataagaggg 
      caagtcagc aattgcgat cagcgtgac ccaaatggc 
      cagaagggc acaatagaa atcggcgaa ctgcaacgc 
      ccccaagcg tgttataca aaaactaat cacgttttc 
      gccagcatc acacgcgtg gtagaaggt tgagaagaa 
      gcaaattgc gaaataaac tatagcttc gacgtagta 
      tttgcgacg gtatgccac atacaccgt cggcagaaa 
      catcaagtt gctgaggac caatagaag aagttcgat 
      gacgatcat gtagtatgg ggtggcctc agaggcgtc 
      cgagcaatc ccagcctga aacaaaaga c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY