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Gene information
General information
TC033708
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009838
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8878516..8897509
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033708
    iBeetle screen iB_08742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033708 RA
      MMYDSKAKP YPLQVHHMY RDRLLKKAP KTVGVWSAW 
      GPWTTCSRS CGGGVTQQT RHCINRPAD TRFVRGQRR 
      RRQANGCVG LYKRIHLCN SQNCPSGRD FRYEQCAAF 
      NHRLFKGKT YVWEPFLNA PDECALNCR ASGLNFYAT 
      LNKTVIDGT SCSRPLTSY GRPAPRGTR GVCIEGSCK 
      AWLEEAEAQ AKNKRRDPD APSVLPAGE KKRKRTSIA 
      APEKRSLEA YFAVQPRPS GRRELCASG GNSQHPKIV 
      SIALFYRNI KAKFRLG
    CDS sequence
    • >TC033708 RA
      atgatgtac gactccaaa gccaagccg tatccactt 
      caagtgcac cacatgtac cgcgaccgt ctgctgaag 
      aaagcgccg aagacggtg ggagtgtgg tcagcatgg 
      gggccctgg acgacgtgt tcgagaagc tgtgggggt 
      ggcgtcacg caacagacg agacattgt atcaatcga 
      cctgctgat acgagattt gttcgagga caaaggcgg 
      agaagacag gccaatggt tgcgtgggt ttgtataaa 
      aggattcat ctctgcaat tcacagaat tgtccgtcg 
      ggaagggac tttcggtac gaacaatgt gccgcattc 
      aaccacagg ctgttcaag gggaaaacc tacgtttgg 
      gaacctttc ctcaatgca cctgacgaa tgtgcgctc 
      aattgtcga gcttccggc ttgaatttt tacgccacc 
      ctcaacaag accgtgatc gacggcacc tcctgcagc 
      cgccccttg acatcttac ggccggcct gcccccaga 
      ggcactcgt ggtgtctgt atcgaggga tcttgcaag 
      gcctggctg gaggaggcc gaagcccag gccaagaac 
      aagagacgg gacccggac gcccccagt gtactacca 
      gcaggtgag aaaaagcgc aagaggacg tcaattgca 
      gcgccggag aagcgcagt ctggaggcc tacttcgca 
      gtgcaaccg cgaccttca ggaagacgt gaattgtgc 
      gcgagtggt ggcaattcg cagcatccc aaaatcgtg 
      tccattgct ctgttttat cgaaatatc aaagcaaag 
      ttcaggttg ggatag
    mRNA sequence
    • >TC033708 RA
      ttctcctct tcgacctgg ggatgatgt acgactcca 
      aagccaagc cgtatccac ttcaagtgc accacatgt 
      accgcgacc gtctgctga agaaagcgc cgaagacgg 
      tgggagtgt ggtcagcat gggggccct ggacgacgt 
      gttcgagaa gctgtgggg gtggcgtca cgcaacaga 
      cgagacatt gtatcaatc gacctgctg atacgagat 
      ttgttcgag gacaaaggc ggagaagac aggccaatg 
      gttgcgtgg gtttgtata aaaggattc atctctgca 
      attcacaga attgtccgt cgggaaggg actttcggt 
      acgaacaat gtgccgcat tcaaccaca ggctgttca 
      aggggaaaa cctacgttt gggaacctt tcctcaatg 
      cacctgacg aatgtgcgc tcaattgtc gagcttccg 
      gcttgaatt tttacgcca ccctcaaca agaccgtga 
      tcgacggca cctcctgca gccgcccct tgacatctt 
      acggccggc ctgccccca gaggcactc gtggtgtct 
      gtatcgagg gatcttgca aggcctggc tggaggagg 
      ccgaagccc aggccaaga acaagagac gggacccgg 
      acgccccca gtgtactac cagcaggtg agaaaaagc 
      gcaagagga cgtcaattg cagcgccgg agaagcgca 
      gtctggagg cctacttcg cagtgcaac cgcgacctt 
      caggaagac gtgaattgt gcgcgagtg gtggcaatt 
      cgcagcatc ccaaaatcg tgtccattg ctctgtttt 
      atcgaaata tcaaagcaa agttcaggt tgggatagg 
      tcgggttat tgattcgtg aatttggaa atgtcaagt 
      gaaaacatg tcgcgagat aaacaccaa actcataaa 
      tatttatcc gattgcgaa acggattta cattaataa 
      agctatttc gtgaaaatt tttcgatcg gtgttgggt 
      ttcattggg aagtttttt ccgttggcg cgtgttttc 
      attttcgca tattttatg aattatttg acattgaaa 
      tttttttca caggaaaaa tatttgttg tcgcggata 
      atagcgatt ggagttcaa caaactacc aaactatgc 
      ggcaaataa tttatggta gtgtaattt aattagata 
      ttttcaaag tcacggagt tgcgacatt ttaacgcga 
      aaaaatcca cacgcggcg cttccgcaa atctgcaac 
      tgatttttt caattgaat tccattttg aagtaggta 
      attctaaaa atagattta cagaaacgt aaatagact 
      cccaaatta acttcgatt cggtttttt tcacaattt 
      tcgaaccgc cactgtcga agtgaaaaa tggaaacat 
      acttaactt cataaatta tatttccca attattact 
      taattggaa ttaacattt atgaataca tttgcattt 
      cccgattta tcactgtaa ttaatctgc aattaggca 
      caattaaaa aagtaatcg ttcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY