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Gene information
General information
TC009818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8589916..8608070
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009818
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08737

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009818 RA
      MHPSRRRKK RPNYGIRTV EVLRGSNGF GFTISGQQP 
      CILSCIVAN SPADHAGLR AGDFLISVN GISVSKITH 
      DAVVNLIGN CVGPIRMTI AENYYSDSS EEELDVSRM 
      MGARKPKYM HKPRPHRCY KQSPSENTK KHNENIKYN 
      DENSGNLLN ISNQMSMED EAGPVEYKA LVGYLGTIE 
      MPKQLLPNS RLQTVCSCI KKLRQEKRA PTAVLMTIF 
      PTCLTLKNS SNHILAIYP TNRVVYIGS TADRESRYF 
      GLVTSAVSD MRSTENLKH FQWSEDKKF NNTEPEIEI 
      SNSCHVFMI DPKIVDHSV HAKKADTFK FSCTTDVIT 
      GGCLEFPRN ALYIVNLVQ TMYKLQNNE SPKNDEASL 
      GPLVANSPQ PSASSNSDS GIGFRDDCG NISDRILVV 
      EFPTHRPFP IVNATNNRP SGIDASSIP LESLDLPLN 
      NEINNKNHD IKSKQLNNL NNIRACGSV NIKNVLGKN 
      DNHFLTKQN NDGEVYNRL TCRAMPDRP MRVTRGLSP 
      TSSDDNTKV PDSKSSETL SVSEDVDFQ SSVDNISTH 
      SSKSDLKGV FKAPSMFFF ENKKLKRQS KMVNSCDNL 
      DKFEENIMN YKLSPKVYG LSKPNHSCE ELNNFDTVE 
      KYGYGSLQE LCRWDGQGK MRNIAQSEP DVRMQRNGM 
      ECEKNGPKE SEGPAGWAS SFEKLLDDP LGLHTFAEF 
      LKKEFSAEN IYFWTACER YRRLPAGPA RVAEAQRIY 
      QQHLCVGAP EGVNVDSHG RHCTEQALQ EASEALFDQ 
      AQKQIFNLM KFDSYPRFL KSSLYKQSL AGELERPQI 
      DSGLLLQPP TPTKLKKSL SNAEDRRRK SLLPWHRKN 
      RSKSKDRGE SEYCQKRGD IVTSCESVN GKLGGGSDT 
      HSSKSSLTS LDLAITSTQ DLSKGSQDE TSSTVARGP 
      LCRVVLSNG STTVVQIKT TESIQQLVT RLLDKRGIV 
      YSAFEVYTN KHQKALDVT DPSTKLAGC EVTIEQRVT 
      FKLDLPNKK VISVKSKHT KIIADVLRP ILQKYNYNL 
      DQAKVTIGD TEAVDIHLP VTSIDEKRL RITYCEKTV 
      PATPVIKVN NVKAKLDEI TNQVFEDIL QEKADSAFL 
      KAKSDKGSV KSEDWGSEH SSGIFGRFL RRDSGLHDR 
      KKKSLMKLK PPGNSSDDV TGEQVKKPL IAKWKTGVN 
      KLQVACSES DELVEGLTR AQRSRLEDQ RGTEINFEL 
      PDFLKDKNS RFFVENGDL RRNNYENRN IMPKDFNNL 
      TNNNNNNSF TNHNQKLNN LRIYSPNKF SSDESSPSK 
      SLENTVIEN KKDSSKCEP PPLPPKPKI VPIKPPNWG 
      QNGYYKKEL GASDKKMYL EQPSSSFV
    CDS sequence
    • >TC009818 RA
      atgcacccc agtcggcgg cgcaaaaag cgccccaac 
      tacggcatc cggaccgtg gaagtgctg cggggctct 
      aacggcttc ggcttcacc atctcgggc cagcagccc 
      tgcattttg tcgtgtatt gtggcgaat tcccccgcg 
      gatcacgcg gggctccgc gctggtgac ttcctcatc 
      tccgtcaac ggcatctcc gtgtcgaaa atcacacac 
      gatgccgtg gttaacctc atcggtaac tgcgtgggg 
      cccatcagg atgaccata gcggagaat tactactcg 
      gacagcagt gaagaggag ctggacgtg agtcgcatg 
      atgggggcc cgcaaaccc aaatacatg cacaagccc 
      agaccgcac aggtgctac aagcagtcc ccgagcgaa 
      aacacgaaa aagcacaac gaaaatatc aaatacaac 
      gacgaaaat tcgggaaat ttgctcaac atttcgaat 
      caaatgtcc atggaagat gaggcgggg cccgtggag 
      tacaaggcc ctggttggc tatttagga acaattgaa 
      atgcccaaa caactgttg ccaaactcg agactgcaa 
      acagtttgc agttgcatt aaaaaattg aggcaggaa 
      aaacgggcc cccaccgct gtcctaatg acgatattc 
      ccgacttgc ctaacactg aaaaattcc tcaaaccac 
      attcttgcc atatacccc acaaaccgg gtcgtttac 
      atcggaagc accgccgac agggaatct cgttatttc 
      gggttggta acaagtgcc gtttccgac atgagaagt 
      accgaaaat ctcaagcac ttccaatgg tccgaagac 
      aaaaaattc aacaacacg gaaccggaa attgaaata 
      tcgaacagt tgtcacgtg tttatgata gacccgaaa 
      attgtggac cacagtgtc catgccaaa aaagccgac 
      acgttcaaa ttttcctgc acaactgac gtcatcacc 
      ggaggctgc ctcgaattt ccccggaac gccctgtac 
      atagtaaac ctcgtgcaa acaatgtac aaactacag 
      aataacgaa agcccgaaa aatgacgag gcgagcttg 
      ggccccctt gtcgcgaac tccccccag cccagtgct 
      tcgagcaat tccgactcc gggatcggc ttccgggat 
      gattgcggg aatatcagc gaccggatt ctggtagtg 
      gaattcccg acgcatcgg ccgttcccg attgtcaat 
      gctacgaac aatcggccg tctggaatc gacgcttcg 
      agtatcccc cttgagtcg ttagacctg cccctgaat 
      aacgaaatc aacaacaag aatcatgac atcaagtcc 
      aaacagtta aataacttg aataacatt cgagcgtgt 
      gggtccgtc aacattaag aatgttctg ggaaagaat 
      gacaatcac tttttgacg aaacaaaac aacgacggg 
      gaggtttac aaccggttg acttgtcgg gcgatgcct 
      gataggccc atgagggtc acaaggggg ctgtcaccg 
      accagttcc gacgacaac acgaaagtc cccgattcg 
      aaatcgtca gagacactc tcagtgtcc gaagacgtc 
      gattttcag agttcggtt gataacatt tcgacccac 
      agctcgaaa agtgaccta aaaggggtt ttcaaagcc 
      cccagtatg tttttcttc gagaataaa aaattgaaa 
      aggcagagt aagatggtg aatagttgc gacaatttg 
      gataagttt gaggagaat attatgaat tataaattg 
      agtccgaaa gtttacgga ttgtcgaaa cccaatcat 
      agctgtgag gagttgaat aattttgat acagttgag 
      aagtatggc tatggaagt ttgcaggag ttgtgtaga 
      tgggatggg caaggaaaa atgagaaat atcgcacag 
      agtgaaccc gatgtgaga atgcagagg aacggaatg 
      gagtgtgaa aaaaatggc cccaaggag tcggaaggg 
      cccgccgga tgggcctcg tccttcgag aaactcctc 
      gacgatccc ttaggactc cacacgttt gcggaattt 
      ttgaaaaag gaatttagt gccgaaaat atttacttc 
      tggactgcg tgcgaacgc tatcggcga cttccggca 
      ggtccggct cgagtcgcc gaagcccag cgaatctac 
      cagcaacat ttgtgcgtg ggagccccc gagggcgtc 
      aatgtggac tcgcacggg cgccactgc actgaacaa 
      gcgctacaa gaagccagc gaagcgctt tttgaccaa 
      gcgcaaaag cagattttt aatttaatg aagtttgat 
      agttatccg cgatttctc aaatcgagt ctttacaaa 
      caaagtctt gccggcgaa ctagagagg cctcagatc 
      gattcagga ttgttatta caaccccca acgcccacg 
      aaattaaag aaatcgtta agtaatgcc gaagatagg 
      agaaggaag tcacttttg ccgtggcat cggaaaaac 
      cggtccaag tcaaaagac cgaggcgag tccgagtat 
      tgtcaaaag aggggtgat attgttaca agttgcgag 
      agtgtgaat ggaaaattg gggggcggg agcgacaca 
      cacagcagt aagtcgtcg ctgacgtcg ctggatttg 
      gcgattacg tccacacag gatttgagt aaaggaagt 
      caggacgaa acctcgtcg acggtggcc cgagggccc 
      ctctgtaga gtagttctg agtaacgga agcacgaca 
      gtagtacaa atcaaaaca accgaaagt attcaacag 
      ttggtcact agactatta gataaacga gggattgtt 
      tattcagcg ttcgaagtt tacacaaac aaacaccaa 
      aaggcactt gatgtcacc gatccgtcg acaaaatta 
      gccggctgt gaggtaaca atcgagcag cgagtgact 
      tttaaacta gacctcccg aacaagaaa gtcatttcg 
      gtgaaatcc aaacatacg aaaataatt gcggacgtg 
      ttacgaccg atcttgcaa aaatacaac tacaatttg 
      gaccaagcg aaagtcaca attggggac accgaagcc 
      gtggatatt cacttgccg gtcacttcg attgatgaa 
      aaacgcctc cgaatcacc tattgtgaa aaaacagta 
      cctgcgact cccgttatt aaagtgaac aacgtgaaa 
      gcgaaactc gacgagatt accaaccaa gtattcgaa 
      gatattctc caagagaag gccgattcg gcgtttctc 
      aaagcaaag tcggataag gggtctgtg aaaagcgag 
      gactggggc tcggagcac tcgtcgggg attttcggg 
      aggtttctg cggcgcgat tccgggctc cacgaccgg 
      aagaaaaag tcgctgatg aagctaaaa cccccggga 
      aacagtagc gatgatgtg acgggggag caagtgaaa 
      aagccgcta atagcaaaa tggaaaact ggagtgaat 
      aaattacaa gtggcttgt agcgaaagc gacgaactg 
      gtcgagggt ctgacaagg gcgcagagg tcgcggttg 
      gaggaccag cgcgggact gagataaat ttcgaactt 
      cctgatttt ttgaaagat aagaatagc cggttcttt 
      gtcgaaaat ggggacctc agacggaat aattacgag 
      aacaggaat atcatgccc aaggatttt aacaatttg 
      actaataat aacaacaat aattcgttt acaaaccac 
      aatcagaaa ctgaataat ttgcgaatc tactcgccg 
      aataaattt tcgtcggat gagagttcg ccgtcgaaa 
      agcttggaa aatactgtt attgaaaat aagaaagac 
      agttcgaag tgtgaacca ccgccactg cccccgaaa 
      cctaaaatc gttcctata aaaccgcca aattggggc 
      cagaacggg tactataag aaagaattg ggcgcgagt 
      gataagaaa atgtatcta gaacagccg tcgagcagc 
      tttgtctaa
    mRNA sequence
    • >TC009818 RA
      acggccatt taaaaactg tcgaaatca agctgtgac 
      gtgtggcaa gggccgttc gccccagtg tttacgtgt 
      ttgtgtttc gtaacctaa aatgcaccc cagtcggcg 
      gcgcaaaaa gcgccccaa ctacggcat ccggaccgt 
      ggaagtgct gcggggctc taacggctt cggcttcac 
      catctcggg ccagcagcc ctgcatttt gtcgtgtat 
      tgtggcgaa ttcccccgc ggatcacgc ggggctccg 
      cgctggtga cttcctcat ctccgtcaa cggcatctc 
      cgtgtcgaa aatcacaca cgatgccgt ggttaacct 
      catcggtaa ctgcgtggg gcccatcag gatgaccat 
      agcggagaa ttactactc ggacagcag tgaagagga 
      gctggacgt gagtcgcat gatgggggc ccgcaaacc 
      caaatacat gcacaagcc cagaccgca caggtgcta 
      caagcagtc cccgagcga aaacacgaa aaagcacaa 
      cgaaaatat caaatacaa cgacgaaaa ttcgggaaa 
      tttgctcaa catttcgaa tcaaatgtc catggaaga 
      tgaggcggg gcccgtgga gtacaaggc cctggttgg 
      ctatttagg aacaattga aatgcccaa acaactgtt 
      gccaaactc gagactgca aacagtttg cagttgcat 
      taaaaaatt gaggcagga aaaacgggc ccccaccgc 
      tgtcctaat gacgatatt cccgacttg cctaacact 
      gaaaaattc ctcaaacca cattcttgc catataccc 
      cacaaaccg ggtcgttta catcggaag caccgccga 
      cagggaatc tcgttattt cgggttggt aacaagtgc 
      cgtttccga catgagaag taccgaaaa tctcaagca 
      cttccaatg gtccgaaga caaaaaatt caacaacac 
      ggaaccgga aattgaaat atcgaacag ttgtcacgt 
      gtttatgat agacccgaa aattgtgga ccacagtgt 
      ccatgccaa aaaagccga cacgttcaa attttcctg 
      cacaactga cgtcatcac cggaggctg cctcgaatt 
      tccccggaa cgccctgta catagtaaa cctcgtgca 
      aacaatgta caaactaca gaataacga aagcccgaa 
      aaatgacga ggcgagctt gggccccct tgtcgcgaa 
      ctcccccca gcccagtgc ttcgagcaa ttccgactc 
      cgggatcgg cttccggga tgattgcgg gaatatcag 
      cgaccggat tctggtagt ggaattccc gacgcatcg 
      gccgttccc gattgtcaa tgctacgaa caatcggcc 
      gtctggaat cgacgcttc gagtatccc ccttgagtc 
      gttagacct gcccctgaa taacgaaat caacaacaa 
      gaatcatga catcaagtc caaacagtt aaataactt 
      gaataacat tcgagcgtg tgggtccgt caacattaa 
      gaatgttct gggaaagaa tgacaatca ctttttgac 
      gaaacaaaa caacgacgg ggaggttta caaccggtt 
      gacttgtcg ggcgatgcc tgataggcc catgagggt 
      cacaagggg gctgtcacc gaccagttc cgacgacaa 
      cacgaaagt ccccgattc gaaatcgtc agagacact 
      ctcagtgtc cgaagacgt cgattttca gagttcggt 
      tgataacat ttcgaccca cagctcgaa aagtgacct 
      aaaaggggt tttcaaagc ccccagtat gtttttctt 
      cgagaataa aaaattgaa aaggcagag taagatggt 
      gaatagttg cgacaattt ggataagtt tgaggagaa 
      tattatgaa ttataaatt gagtccgaa agtttacgg 
      attgtcgaa acccaatca tagctgtga ggagttgaa 
      taattttga tacagttga gaagtatgg ctatggaag 
      tttgcagga gttgtgtag atgggatgg gcaaggaaa 
      aatgagaaa tatcgcaca gagtgaacc cgatgtgag 
      aatgcagag gaacggaat ggagtgtga aaaaaatgg 
      ccccaagga gtcggaagg gcccgccgg atgggcctc 
      gtccttcga gaaactcct cgacgatcc cttaggact 
      ccacacgtt tgcggaatt tttgaaaaa ggaatttag 
      tgccgaaaa tatttactt ctggactgc gtgcgaacg 
      ctatcggcg acttccggc aggtccggc tcgagtcgc 
      cgaagccca gcgaatcta ccagcaaca tttgtgcgt 
      gggagcccc cgagggcgt caatgtgga ctcgcacgg 
      gcgccactg cactgaaca agcgctaca agaagccag 
      cgaagcgct ttttgacca agcgcaaaa gcagatttt 
      taatttaat gaagtttga tagttatcc gcgatttct 
      caaatcgag tctttacaa acaaagtct tgccggcga 
      actagagag gcctcagat cgattcagg attgttatt 
      acaaccccc aacgcccac gaaattaaa gaaatcgtt 
      aagtaatgc cgaagatag gagaaggaa gtcactttt 
      gccgtggca tcggaaaaa ccggtccaa gtcaaaaga 
      ccgaggcga gtccgagta ttgtcaaaa gaggggtga 
      tattgttac aagttgcga gagtgtgaa tggaaaatt 
      ggggggcgg gagcgacac acacagcag taagtcgtc 
      gctgacgtc gctggattt ggcgattac gtccacaca 
      ggatttgag taaaggaag tcaggacga aacctcgtc 
      gacggtggc ccgagggcc cctctgtag agtagttct 
      gagtaacgg aagcacgac agtagtaca aatcaaaac 
      aaccgaaag tattcaaca gttggtcac tagactatt 
      agataaacg agggattgt ttattcagc gttcgaagt 
      ttacacaaa caaacacca aaaggcact tgatgtcac 
      cgatccgtc gacaaaatt agccggctg tgaggtaac 
      aatcgagca gcgagtgac ttttaaact agacctccc 
      gaacaagaa agtcatttc ggtgaaatc caaacatac 
      gaaaataat tgcggacgt gttacgacc gatcttgca 
      aaaatacaa ctacaattt ggaccaagc gaaagtcac 
      aattgggga caccgaagc cgtggatat tcacttgcc 
      ggtcacttc gattgatga aaaacgcct ccgaatcac 
      ctattgtga aaaaacagt acctgcgac tcccgttat 
      taaagtgaa caacgtgaa agcgaaact cgacgagat 
      taccaacca agtattcga agatattct ccaagagaa 
      ggccgattc ggcgtttct caaagcaaa gtcggataa 
      ggggtctgt gaaaagcga ggactgggg ctcggagca 
      ctcgtcggg gattttcgg gaggtttct gcggcgcga 
      ttccgggct ccacgaccg gaagaaaaa gtcgctgat 
      gaagctaaa acccccggg aaacagtag cgatgatgt 
      gacggggga gcaagtgaa aaagccgct aatagcaaa 
      atggaaaac tggagtgaa taaattaca agtggcttg 
      tagcgaaag cgacgaact ggtcgaggg tctgacaag 
      ggcgcagag gtcgcggtt ggaggacca gcgcgggac 
      tgagataaa tttcgaact tcctgattt tttgaaaga 
      taagaatag ccggttctt tgtcgaaaa tggggacct 
      cagacggaa taattacga gaacaggaa tatcatgcc 
      caaggattt taacaattt gactaataa taacaacaa 
      taattcgtt tacaaacca caatcagaa actgaataa 
      tttgcgaat ctactcgcc gaataaatt ttcgtcgga 
      tgagagttc gccgtcgaa aagcttgga aaatactgt 
      tattgaaaa taagaaaga cagttcgaa gtgtgaacc 
      accgccact gcccccgaa acctaaaat cgttcctat 
      aaaaccgcc aaattgggg ccagaacgg gtactataa 
      gaaagaatt gggcgcgag tgataagaa aatgtatct 
      agaacagcc gtcgagcag ctttgtcta attgtatca 
      tactgtata gataaccga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY