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Gene information
General information
TC009809 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8494286..8495691
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009809
    iBeetle screen iB_08736

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009809 RA
      MFGCGRPKN RENDEEREH DRRFKRSTK LLLLGTGES 
GKTTIIKQM KILHINGFS TEEKKQKIP YIKQNIHES 
IYDIVYNMD KIDPPVKLE KSGNEECAK FILDLGPEE 
LEEYTEEYF DNVKSLWND QGVQKTFER SNEYQLIDS 
AQHFLDRID EIRKPDYIP STQDILFCR IRTTSISEI 
EFSVPVPKT YGGGSANFL MYDVGGQRG ERRKWIQVF 
DGIDAILFI IAASDFDQT LREDESKNR LEEALSVFR 
DMCGSRFLR KAGMIVFLN KQDLFQRKI EHGRKLEKY 
FPEYKNFNK TLGSAVDNA KAFLEEKIT QMSKIQVTV 
KNYNPITKT VTEEKLPPR DIYIHHTIA TDTQNVKKV 
FDSVHEMII KESVKNIAS Y
    CDS sequence
    • >TC009809 RA
      atgtttggt tgtgggcgt cccaaaaat agagaaaac 
gatgaagaa agagaacat gacagacgt ttcaaaaga 
tcgaccaaa ttactactt ttgggtaca ggtgaatct 
ggaaaaact acaatcatt aaacaaatg aaaattttg 
cacattaat ggattttca accgaagaa aagaaacaa 
aaaattcca tacatcaag caaaatatt cacgagtct 
atctatgat attgtttac aacatggat aaaattgat 
cctcctgta aaactggag aagagcgga aatgaagaa 
tgcgccaaa tttatttta gacttgggg ccagaagaa 
ctagaggaa tacactgag gaatatttt gataacgtg 
aagagcctt tggaacgat caaggggtg caaaaaacg 
ttcgagcgc tcaaacgaa taccagtta atagacagc 
gcccaacat ttcttagac cgtattgac gaaattagg 
aagccggac tacatcccc tcaactcaa gacattctg 
ttttgccga attcgcacc acttccatt tcggagatt 
gagttcagc gtcccagtc cctaaaaca tacgggggc 
ggaagcgcc aatttcctc atgtacgat gtagggggc 
cagcggggg gaacgccgc aaatggata caagttttc 
gacggaatt gacgcaatt ttattcata atcgcggcc 
agtgatttc gatcaaaca ctacgagaa gatgaatcg 
aaaaatcgg cttgaggaa gctttgtcc gttttcaga 
gacatgtgt ggaagccgg tttttacga aaggctgga 
atgattgtt tttctcaac aaacaggac ctcttccag 
aggaaaata gagcacggg agaaaattg gaaaagtac 
ttccccgag tacaaaaat tttaataaa actctgggt 
tctgcggtg gataatgcg aaagccttc ctcgaggag 
aagataaca caaatgtcg aaaatacaa gtaacagtg 
aaaaattat aatccaatt acaaaaaca gtaactgag 
gagaagctg ccccctagg gatatttat atccaccac 
actatcgct acagacact cagaatgtt aaaaaagtg 
tttgatagc gtccacgaa atgataatt aaggaaagt 
gtaaaaaac attgcaagt tactaa
    mRNA sequence
    • >TC009809 RA
      acctggtta aaaaataaa aatgtttgg ttgtgggcg 
tcccaaaaa tagagaaaa cgatgaaga aagagaaca 
tgacagacg tttcaaaag atcgaccaa attactact 
tttgggtac aggtgaatc tggaaaaac tacaatcat 
taaacaaat gaaaatttt gcacattaa tggattttc 
aaccgaaga aaagaaaca aaaaattcc atacatcaa 
gcaaaatat tcacgagtc tatctatga tattgttta 
caacatgga taaaattga tcctcctgt aaaactgga 
gaagagcgg aaatgaaga atgcgccaa atttatttt 
agacttggg gccagaaga actagagga atacactga 
ggaatattt tgataacgt gaagagcct ttggaacga 
tcaaggggt gcaaaaaac gttcgagcg ctcaaacga 
ataccagtt aatagacag cgcccaaca tttcttaga 
ccgtattga cgaaattag gaagccgga ctacatccc 
ctcaactca agacattct gttttgccg aattcgcac 
cacttccat ttcggagat tgagttcag cgtcccagt 
ccctaaaac atacggggg cggaagcgc caatttcct 
catgtacga tgtaggggg ccagcgggg ggaacgccg 
caaatggat acaagtttt cgacggaat tgacgcaat 
tttattcat aatcgcggc cagtgattt cgatcaaac 
actacgaga agatgaatc gaaaaatcg gcttgagga 
agctttgtc cgttttcag agacatgtg tggaagccg 
gtttttacg aaaggctgg aatgattgt ttttctcaa 
caaacagga cctcttcca gaggaaaat agagcacgg 
gagaaaatt ggaaaagta cttccccga gtacaaaaa 
ttttaataa aactctggg ttctgcggt ggataatgc 
gaaagcctt cctcgagga gaagataac acaaatgtc 
gaaaataca agtaacagt gaaaaatta taatccaat 
tacaaaaac agtaactga ggagaagct gccccctag 
ggatattta tatccacca cactatcgc tacagacac 
tcagaatgt taaaaaagt gtttgatag cgtccacga 
aatgataat taaggaaag tgtaaaaaa cattgcaag 
ttactaata caaatttat tacaagttt acacaaata 
ctttactta agcaaagac cacttgagt tgctctttg 
gccgactgt attacctat aaataaacg tatttgtta 
tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY