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Gene information
General information
TC009803 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8324450..8335124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009803
    iBeetle screen iB_08734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009803 RA
      MKFLLEAND GGCYKWSTT RNDIIKLTM LDENLDLHC 
      SIKAEVQTV TKEPVRNLA VVLAEDVQT DQILKCDVV 
      VDAISSLSI TTTTRELFM EEVPEAFQV NAYDAQGNE 
      FSSLEGVEF DWKIITLGS KKEAVVVRY LPFKDSPYE 
      SLPHIQKLE DDNRKGSVI LLEGVKTGS AKVSVRLPY 
      SEYKEVPSI EVVLTVIAN LLLVPSEAY MMEGDVISF 
      KLYYMHNGK MDELQIPDA QYFLESEDD SVVKSDKSS 
      REVTARALG KCHVMLRSS NRLDDDPAL KLPVAVITV 
      VQPSYIVLT ILPHKNWAI LVGDQHEII AEVYSSSDN 
      KLYLGSGVQ IQTQVGEAF HVEERSQNG SWISGWGLK 
      ESRAIVSAK LEAVIHPTL GKFTLDTPI ISQGELYIY 
      PRITISPAE VVLPWDTQI KPKYDIDLV AKGGDGRFL 
      WSSTDHSIG IVSQTGHVR TLTQGYFSV SAAMTRNHH 
      NRQYAKFSI LPPVRLEIV EFIMEAEIF QPIYLHIAL 
      YAPKTSNDN TTQTFIPFT QCQDLPFQV KQTDNKFIY 
      NKTASIPPV GISCGNIAM IGTAVGTSK VTVTYYQDG 
      LILEDSVTI NAYYPLKLI SPSPKHQVV LAVGSAIHL 
      VFSGGPRPQ IGRQSDHQR IVIVENEAT VQAEDVTDS 
      NQLPLEDIT AVWVLCRKL GETNVKLTI SNTPSLPNC 
      KSHGSTVTT KVICGKPRR LHIQPEVKV NDAKACPMD 
      LSADRVVVP NNQDIELEV VVIDELGRV FLNTSSLLF 
      TWESRPAGE VKFQSIDST ITKHEMFGS VPLVNKTLQ 
      IIRPYIDSG LLEVTGTVK GYKMSVLKA YHITPEWPE 
      FLSGEERSA DHPAITATV GLFLVDDTV VTPNVTIIF 
      NQPNVKKEI AVMHGSGYI ELSLSNDEI ATVNYVEGT 
      RLIEIVPVV SGEMTIQVV DLCLVANPI FIYVKVVSL 
      GKIEVETAG KVEINHCIQ VVAKLYDET DQLLDVHDM 
      GVIELRHRF SKNIANLGR MPQNADDPW PLGEVHFVL 
      TGVELGDAQ LTFTFDNKD QVVSSEPVD IQVFPPLKV 
      NPRNGTLLV GATMQLTLR GGPQPDTSI EFEPHTNGT 
      ITVSDVGIV VGKNLGFTK ITVRSIGVH PKTGEKIIY 
      AEDTIDVNI VKISGVKIG APLTNFKSG GTIPVWVTG 
      IPETISPLI LSSFEDSAL TYDWYFNDF DVTTLVGVF 
      STVGVIYKT CDKTIVRVT GLQPGKTKL YVNVTFNAV 
      DATQPTVYQ AYVDLEVFE PLMLVSPRH MPGRSLLMA 
      RHSSIQLQT NLEGIVNIE YSIPEFRLT GTEVTAMNS 
      TPVATITVT ETGYLQSFG TVGFAQLLI TATDELGLK 
      QTLTYVVEV KPVHYMLLT VKANWRIHL DSSLQVIPL 
      GTEFDLVAN FYDNIGNKF NAGPRQVKV RANRLDLVK 
      IKQYTSNAT MTVATKKPG HTVIKAWTD GVDNTADYV 
      KIHSKEVVK PVVDFLTSG DIMCLWSPV VTRHLQIGF 
      WSSSDTSMI SIDATIDLA FVIGNKEGT VLLTHSLQA 
      NVPLRMQVL PVAEVKLYP DKVTFFTNG AEGMVQRIG 
      VVLKSRVGP ADKRNNLIQ GWLCDRSFH RYLVPTPFR 
      CFVRFTNES VDIDANSIF SMKSSFKPE IGQYNCKII 
      STGANTSDV SLLMTNITF WVISDEGEI ESQPLEMPF 
      IPTPYVPPE VYIGDQGNV GELVVTGLG YILEHIVVQ 
      PADSSIVYV DKGRLIDEN HKMYQVQLV DYHPKFADQ 
      EDVMGIIVR SPLTDQATQ VMVRVSNKY RDDTCAGKS 
      PIYRFIQHY KHAIIIVIS MLIIFCVTC YVYSRYMQP 
      MVTVTVNPN RSLLNASYQ QQHYPTAST PMNMMNRTQ 
      PSIPRLSPT SPICTSKSN CTCGRNREP VYGDVSSFT 
      VNSPELRRN RRFL
    CDS sequence
    • >TC009803 RA
      atgaaattt ctgctggaa gccaacgat ggcggttgt 
      tacaaatgg agcacgact cgcaatgat atcatcaaa 
      ttgacaatg cttgacgaa aatcttgac ttacattgc 
      tccataaag gccgaagtt caaactgtg accaaagag 
      ccggtcagg aacctcgct gtggttctt gccgaggat 
      gttcaaacc gaccaaata ctcaaatgc gacgttgtg 
      gtcgacgct ataagttcc ttatcaatt acgacaacg 
      acaagggag ctttttatg gaagaagtt cccgaggcg 
      ttccaagta aatgcttat gacgcacaa ggcaacgag 
      ttttcctcc ctggaaggg gtggaattt gactggaag 
      atcatcaca cttggcagc aaaaaggaa gcggtggtt 
      gtgagatac ctgccgttt aaagactcc ccctatgag 
      tcactacct cacatccag aaactcgaa gatgacaat 
      agaaaaggc tcagttatt ttacttgaa ggcgtgaaa 
      acgggctca gctaaagtt tcagtacgt cttccgtat 
      tcggaatac aaagaagtt ccaagtatt gaagtcgtt 
      ttgaccgtc attgctaat ttgttgctt gttccgagt 
      gaggcttat atgatggag ggagatgtg atctcattt 
      aaattatac tatatgcat aatggtaaa atggatgag 
      ttgcaaata ccggacgcc caatatttc ctcgaatct 
      gaagatgat agtgttgtt aaaagtgat aaatcttca 
      agggaagtt actgccagg gcgttgggt aaatgtcac 
      gtgatgttg cgcagctcc aataggctg gatgatgac 
      ccagcctta aagcttcca gtggctgtg ataactgta 
      gtacagccc agttacatt gttttgacc attttacct 
      cacaaaaac tgggcaatt ttggtcgga gaccagcat 
      gagattatc gctgaggtg tacagcagc tctgataat 
      aagctctat ctcggttcg ggtgtgcaa atccaaacc 
      caggtgggc gaagccttc cacgtggaa gagcgttcg 
      cagaatggc agctggatt agcgggtgg ggccttaaa 
      gaaagccga gccatagtt tcagctaaa ttagaggcg 
      gtgatccat cccacattg ggcaaattt accctggat 
      acacccata atatcgcaa ggcgagctc tacatctac 
      ccccgaata actatatct cctgccgag gttgtctta 
      ccatgggac acccaaata aaacccaaa tatgacatc 
      gacttggtg gctaaaggg ggcgacggc cgctttttg 
      tggtcaagc acggaccat tccattggt atagtgtct 
      caaacgggt cacgtgcgt accctcacc cagggctat 
      ttttccgta tcggctgct atgacccgc aaccaccac 
      aaccgtcaa tacgcgaaa ttttcaatc cttccacct 
      gttcgtctc gaaattgtt gaatttata atggaagcg 
      gagattttc caaccgatt tacctccac attgctctc 
      tatgccccc aaaacgagc aacgataat accacacaa 
      acttttatt cctttcacg cagtgtcaa gacctcccg 
      ttccaagta aaacaaacc gataacaaa tttatttac 
      aacaaaaca gcatcgatc cctcctgtg ggtatctcc 
      tgcgggaat atagcaatg attggtact gctgttggt 
      acaagcaaa gttacagtg acctactat caggacggt 
      ttaattttg gaagattcg gtcacgata aacgcgtat 
      taccctttg aaattgatt tcgccgagt cccaaacat 
      caggtggtt ctggcggtg ggcagtgcg atacatttg 
      gtgttttcg gggggacct agaccacag attgggaga 
      cagagtgat catcaaagg attgtaatt gtggagaat 
      gaagccact gtgcaggcg gaggatgtg accgattcg 
      aatcaactg ccgttggag gatattacg gccgtttgg 
      gtcttgtgt aggaagttg ggtgagacc aatgttaag 
      ttgacgatc agcaatacg ccctcgttg cccaattgc 
      aagagtcat gggagtaca gtgacgaca aaggtaatt 
      tgcggtaaa ccccgccgc ctccacatc cagccggaa 
      gtcaaagtc aacgatgcg aaggcatgt cccatggac 
      ctcagtgcc gaccgtgtt gtagtgccc aacaaccaa 
      gatattgaa ctcgaagtg gttgtcatc gacgaactc 
      ggccgtgtc ttcctaaat accagtagt cttctcttc 
      acatgggag tcgcgtccg gctggcgaa gttaaattc 
      caaagcatc gacagcaca atcaccaaa cacgaaatg 
      ttcggctcg gttccactg gtcaacaaa acgctccaa 
      attatccgt ccctatatc gactccggg ctgttggaa 
      gtcaccgga accgtcaag ggctacaag atgagtgtc 
      ttaaaagcc tatcacatc acaccggaa tggccggaa 
      tttttgagt ggggaggag cgatcggcc gatcatccg 
      gcgataaca gcgactgtg gggctgttt ttggttgac 
      gatacagtt gtgacaccc aatgtaacg attattttc 
      aatcaaccg aacgtcaag aaggaaata gcagtgatg 
      catgggtcg ggctatatc gaattgtcg ttaagtaat 
      gacgaaatc gcaacggtg aattatgtc gaagggacg 
      agacttatc gaaattgtc ccagtggtg tcaggagaa 
      atgacaata caagtggtg gacctgtgt ctagtcgca 
      aacccgatt ttcatctac gttaaagtg gtttccttg 
      gggaaaatc gaagtcgaa actgccggg aaagtcgag 
      ataaatcac tgcatccaa gtcgtagcg aaactctac 
      gacgaaact gatcaatta ttggacgtg catgacatg 
      ggggttatc gaattgagg catcggttc agcaaaaat 
      atagccaac ttggggcgc atgccccaa aatgccgac 
      gatccctgg cccttggga gaggttcat ttcgttttg 
      actggagtc gaactcggc gatgcccag ctgaccttc 
      acgttcgac aacaaagac caagtcgtt tcgagcgaa 
      ccggtcgac atccaggtg tttccccca ctgaaggtg 
      aatccccga aacggcact cttttggtg ggggctacc 
      atgcaattg acgcttcgt gggggccca caacccgac 
      accagtatc gaattcgag ccacacacg aatggaacc 
      atcaccgtt agtgatgtg ggtattgtt gttgggaaa 
      aatttagga tttaccaaa atcacagtt cgctcgatt 
      ggagtacat ccaaaaaca ggcgagaag attatttac 
      gccgaagat acaatcgat gttaatatt gttaaaatc 
      agcggagtg aagatagga gcgcctttg acgaacttt 
      aagagtggg gggacgatt ccggtttgg gttactgga 
      attccggaa acgatttcg cccttgatt ttgtcgagt 
      tttgaagat tcggctttg acgtacgac tggtatttt 
      aatgatttt gatgtgacg acgcttgtg ggagttttt 
      tcaactgtg ggtgtaatt tacaaaact tgcgacaaa 
      acaatagta agagtgacg ggtttgcaa cctggcaaa 
      acgaagctc tacgtaaac gtaacattc aacgctgtt 
      gatgcaact caacctaca gtgtaccag gcatacgta 
      gatttggaa gtttttgaa cctttgatg cttgtaagc 
      cctcgacat atgccaggg aggtcactt ttgatggcc 
      cgccattca agcatccaa ctacaaacg aatttagaa 
      ggcattgtt aatatagag tacagcatt cctgaattt 
      cgcctgacg gggaccgaa gtaacagca atgaattcg 
      actccggtt gcgacaata acagtgaca gagactggt 
      tatttacag tctttcggt accgttgga tttgcccaa 
      ctgttgata actgcaacc gacgagttg ggattgaag 
      caaaccctg acttatgtt gttgaagtc aagccggtg 
      cattacatg ttattgacg gtgaaagcg aactggaga 
      attcacttg gacagttct ttgcaagtg ataccgtta 
      ggaactgag ttcgatttg gttgcgaat ttttacgac 
      aatatcggg aataaattc aacgctggg cctcgccag 
      gttaaagtg cgggctaat cgactggat cttgttaaa 
      atcaagcaa tacacaagc aatgccact atgaccgta 
      gcgacgaaa aagcccggt catacggtt atcaaggct 
      tggaccgac ggtgttgac aatacggcg gactatgtc 
      aagattcat tcgaaagaa gtggtcaaa cctgtcgtt 
      gattttttg acaagtggc gatattatg tgtttgtgg 
      agtccagtc gtcacaaga catttgcaa atcggtttt 
      tggtccagt agcgacact agcatgatc tcaattgat 
      gcaaccatt gatttggcg tttgttatt ggaaataag 
      gaaggaact gtcttgctc acacactcg ttacaagcc 
      aacgttccg ctgcgaatg caagtctta ccagtcgct 
      gaagttaaa ttatacccc gacaaggtc acatttttc 
      acaaatggg gccgagggg atggtgcaa aggatcggc 
      gttgtgtta aaaagccgg gtggggcca gccgacaag 
      agaaataat ttgatccaa ggatggctc tgtgatagg 
      tctttccac cgatatcta gtcccgacg ccgttcagg 
      tgtttcgtc cgattcact aacgagagt gtagacatc 
      gatgcgaac agtatcttc agcatgaag agcagtttt 
      aaaccggaa ataggtcaa tataattgt aaaatcatc 
      tcaacgggg gctaacacg tccgatgtg agtctccta 
      atgacgaat atcacgttt tgggtcatt tccgacgaa 
      ggggaaatt gaatcgcaa cctttggaa atgccgttc 
      attcctaca ccttacgta ccaccggaa gtgtatatt 
      ggggatcag ggaaatgtc ggcgagctt gttgttaca 
      ggtctgggc tacattttg gagcatatt gtggtacaa 
      ccagccgat agtagcatt gtttatgtg gataagggc 
      cgcttaatt gatgaaaat cataaaatg tatcaagtg 
      cagcttgtc gattatcat ccgaagttt gccgatcag 
      gaagatgtc atgggaatt attgttcgg tcaccgctt 
      acggatcaa gcgactcaa gttatggtg cgagtttcc 
      aataaatat cgcgacgac acctgtgcg ggtaaatcc 
      ccgatttac cgatttata caacattac aaacacgcc 
      attattatc gtgatttca atgctcatt attttctgt 
      gtcacttgt tacgtctat tcacgatat atgcaacca 
      atggttact gtcacagtt aacccgaac cgatctctt 
      ctcaacgct tcttatcag caacagcat tacccgact 
      gcttcaact cccatgaat atgatgaac aggactcaa 
      ccctcaatt ccaagatta tctccaact tcaccgatt 
      tgtacaagc aagtcgaat tgtacctgt ggtaggaat 
      cgagaaccg gtctatggg gatgttagt tcgtttaca 
      gtgaatagt cccgaattg cgaagaaac cgaagattt 
      ttgtaa
    mRNA sequence
    • >TC009803 RA
      ttgggccta aaatctcaa acaaactta tttgccctt 
      tagaaaagt cttgaaaac ttaaatttc cattgttta 
      catttaaat tcgcacagt ggcgccctc tgcccccgc 
      aaatgtttt taacctaaa aaatgtttt gaatccatg 
      catcggttt ttcgtaaat tatggccgg tgcaaaaat 
      tattacatg tatatttta cacactttt catagcaag 
      tgccttttc gtcaaaatt gaacgtgcc gagagtcct 
      acttcccat tttcaatga cttttctat gaaatttct 
      gctggaagc caacgatgg cggttgtta caaatggag 
      cacgactcg caatgatat catcaaatt gacaatgct 
      tgacgaaaa tcttgactt acattgctc cataaaggc 
      cgaagttca aactgtgac caaagagcc ggtcaggaa 
      cctcgctgt ggttcttgc cgaggatgt tcaaaccga 
      ccaaatact caaatgcga cgttgtggt cgacgctat 
      aagttcctt atcaattac gacaacgac aagggagct 
      ttttatgga agaagttcc cgaggcgtt ccaagtaaa 
      tgcttatga cgcacaagg caacgagtt ttcctccct 
      ggaaggggt ggaatttga ctggaagat catcacact 
      tggcagcaa aaaggaagc ggtggttgt gagatacct 
      gccgtttaa agactcccc ctatgagtc actacctca 
      catccagaa actcgaaga tgacaatag aaaaggctc 
      agttatttt acttgaagg cgtgaaaac gggctcagc 
      taaagtttc agtacgtct tccgtattc ggaatacaa 
      agaagttcc aagtattga agtcgtttt gaccgtcat 
      tgctaattt gttgcttgt tccgagtga ggcttatat 
      gatggaggg agatgtgat ctcatttaa attatacta 
      tatgcataa tggtaaaat ggatgagtt gcaaatacc 
      ggacgccca atatttcct cgaatctga agatgatag 
      tgttgttaa aagtgataa atcttcaag ggaagttac 
      tgccagggc gttgggtaa atgtcacgt gatgttgcg 
      cagctccaa taggctgga tgatgaccc agccttaaa 
      gcttccagt ggctgtgat aactgtagt acagcccag 
      ttacattgt tttgaccat tttacctca caaaaactg 
      ggcaatttt ggtcggaga ccagcatga gattatcgc 
      tgaggtgta cagcagctc tgataataa gctctatct 
      cggttcggg tgtgcaaat ccaaaccca ggtgggcga 
      agccttcca cgtggaaga gcgttcgca gaatggcag 
      ctggattag cgggtgggg ccttaaaga aagccgagc 
      catagtttc agctaaatt agaggcggt gatccatcc 
      cacattggg caaatttac cctggatac acccataat 
      atcgcaagg cgagctcta catctaccc ccgaataac 
      tatatctcc tgccgaggt tgtcttacc atgggacac 
      ccaaataaa acccaaata tgacatcga cttggtggc 
      taaaggggg cgacggccg ctttttgtg gtcaagcac 
      ggaccattc cattggtat agtgtctca aacgggtca 
      cgtgcgtac cctcaccca gggctattt ttccgtatc 
      ggctgctat gacccgcaa ccaccacaa ccgtcaata 
      cgcgaaatt ttcaatcct tccacctgt tcgtctcga 
      aattgttga atttataat ggaagcgga gattttcca 
      accgattta cctccacat tgctctcta tgcccccaa 
      aacgagcaa cgataatac cacacaaac ttttattcc 
      tttcacgca gtgtcaaga cctcccgtt ccaagtaaa 
      acaaaccga taacaaatt tatttacaa caaaacagc 
      atcgatccc tcctgtggg tatctcctg cgggaatat 
      agcaatgat tggtactgc tgttggtac aagcaaagt 
      tacagtgac ctactatca ggacggttt aattttgga 
      agattcggt cacgataaa cgcgtatta ccctttgaa 
      attgatttc gccgagtcc caaacatca ggtggttct 
      ggcggtggg cagtgcgat acatttggt gttttcggg 
      gggacctag accacagat tgggagaca gagtgatca 
      tcaaaggat tgtaattgt ggagaatga agccactgt 
      gcaggcgga ggatgtgac cgattcgaa tcaactgcc 
      gttggagga tattacggc cgtttgggt cttgtgtag 
      gaagttggg tgagaccaa tgttaagtt gacgatcag 
      caatacgcc ctcgttgcc caattgcaa gagtcatgg 
      gagtacagt gacgacaaa ggtaatttg cggtaaacc 
      ccgccgcct ccacatcca gccggaagt caaagtcaa 
      cgatgcgaa ggcatgtcc catggacct cagtgccga 
      ccgtgttgt agtgcccaa caaccaaga tattgaact 
      cgaagtggt tgtcatcga cgaactcgg ccgtgtctt 
      cctaaatac cagtagtct tctcttcac atgggagtc 
      gcgtccggc tggcgaagt taaattcca aagcatcga 
      cagcacaat caccaaaca cgaaatgtt cggctcggt 
      tccactggt caacaaaac gctccaaat tatccgtcc 
      ctatatcga ctccgggct gttggaagt caccggaac 
      cgtcaaggg ctacaagat gagtgtctt aaaagccta 
      tcacatcac accggaatg gccggaatt tttgagtgg 
      ggaggagcg atcggccga tcatccggc gataacagc 
      gactgtggg gctgttttt ggttgacga tacagttgt 
      gacacccaa tgtaacgat tattttcaa tcaaccgaa 
      cgtcaagaa ggaaatagc agtgatgca tgggtcggg 
      ctatatcga attgtcgtt aagtaatga cgaaatcgc 
      aacggtgaa ttatgtcga agggacgag acttatcga 
      aattgtccc agtggtgtc aggagaaat gacaataca 
      agtggtgga cctgtgtct agtcgcaaa cccgatttt 
      catctacgt taaagtggt ttccttggg gaaaatcga 
      agtcgaaac tgccgggaa agtcgagat aaatcactg 
      catccaagt cgtagcgaa actctacga cgaaactga 
      tcaattatt ggacgtgca tgacatggg ggttatcga 
      attgaggca tcggttcag caaaaatat agccaactt 
      ggggcgcat gccccaaaa tgccgacga tccctggcc 
      cttgggaga ggttcattt cgttttgac tggagtcga 
      actcggcga tgcccagct gaccttcac gttcgacaa 
      caaagacca agtcgtttc gagcgaacc ggtcgacat 
      ccaggtgtt tcccccact gaaggtgaa tccccgaaa 
      cggcactct tttggtggg ggctaccat gcaattgac 
      gcttcgtgg gggcccaca acccgacac cagtatcga 
      attcgagcc acacacgaa tggaaccat caccgttag 
      tgatgtggg tattgttgt tgggaaaaa tttaggatt 
      taccaaaat cacagttcg ctcgattgg agtacatcc 
      aaaaacagg cgagaagat tatttacgc cgaagatac 
      aatcgatgt taatattgt taaaatcag cggagtgaa 
      gataggagc gcctttgac gaactttaa gagtggggg 
      gacgattcc ggtttgggt tactggaat tccggaaac 
      gatttcgcc cttgatttt gtcgagttt tgaagattc 
      ggctttgac gtacgactg gtattttaa tgattttga 
      tgtgacgac gcttgtggg agttttttc aactgtggg 
      tgtaattta caaaacttg cgacaaaac aatagtaag 
      agtgacggg tttgcaacc tggcaaaac gaagctcta 
      cgtaaacgt aacattcaa cgctgttga tgcaactca 
      acctacagt gtaccaggc atacgtaga tttggaagt 
      ttttgaacc tttgatgct tgtaagccc tcgacatat 
      gccagggag gtcactttt gatggcccg ccattcaag 
      catccaact acaaacgaa tttagaagg cattgttaa 
      tatagagta cagcattcc tgaatttcg cctgacggg 
      gaccgaagt aacagcaat gaattcgac tccggttgc 
      gacaataac agtgacaga gactggtta tttacagtc 
      tttcggtac cgttggatt tgcccaact gttgataac 
      tgcaaccga cgagttggg attgaagca aaccctgac 
      ttatgttgt tgaagtcaa gccggtgca ttacatgtt 
      attgacggt gaaagcgaa ctggagaat tcacttgga 
      cagttcttt gcaagtgat accgttagg aactgagtt 
      cgatttggt tgcgaattt ttacgacaa tatcgggaa 
      taaattcaa cgctgggcc tcgccaggt taaagtgcg 
      ggctaatcg actggatct tgttaaaat caagcaata 
      cacaagcaa tgccactat gaccgtagc gacgaaaaa 
      gcccggtca tacggttat caaggcttg gaccgacgg 
      tgttgacaa tacggcgga ctatgtcaa gattcattc 
      gaaagaagt ggtcaaacc tgtcgttga ttttttgac 
      aagtggcga tattatgtg tttgtggag tccagtcgt 
      cacaagaca tttgcaaat cggtttttg gtccagtag 
      cgacactag catgatctc aattgatgc aaccattga 
      tttggcgtt tgttattgg aaataagga aggaactgt 
      cttgctcac acactcgtt acaagccaa cgttccgct 
      gcgaatgca agtcttacc agtcgctga agttaaatt 
      ataccccga caaggtcac atttttcac aaatggggc 
      cgaggggat ggtgcaaag gatcggcgt tgtgttaaa 
      aagccgggt ggggccagc cgacaagag aaataattt 
      gatccaagg atggctctg tgataggtc tttccaccg 
      atatctagt cccgacgcc gttcaggtg tttcgtccg 
      attcactaa cgagagtgt agacatcga tgcgaacag 
      tatcttcag catgaagag cagttttaa accggaaat 
      aggtcaata taattgtaa aatcatctc aacgggggc 
      taacacgtc cgatgtgag tctcctaat gacgaatat 
      cacgttttg ggtcatttc cgacgaagg ggaaattga 
      atcgcaacc tttggaaat gccgttcat tcctacacc 
      ttacgtacc accggaagt gtatattgg ggatcaggg 
      aaatgtcgg cgagcttgt tgttacagg tctgggcta 
      cattttgga gcatattgt ggtacaacc agccgatag 
      tagcattgt ttatgtgga taagggccg cttaattga 
      tgaaaatca taaaatgta tcaagtgca gcttgtcga 
      ttatcatcc gaagtttgc cgatcagga agatgtcat 
      gggaattat tgttcggtc accgcttac ggatcaagc 
      gactcaagt tatggtgcg agtttccaa taaatatcg 
      cgacgacac ctgtgcggg taaatcccc gatttaccg 
      atttataca acattacaa acacgccat tattatcgt 
      gatttcaat gctcattat tttctgtgt cacttgtta 
      cgtctattc acgatatat gcaaccaat ggttactgt 
      cacagttaa cccgaaccg atctcttct caacgcttc 
      ttatcagca acagcatta cccgactgc ttcaactcc 
      catgaatat gatgaacag gactcaacc ctcaattcc 
      aagattatc tccaacttc accgatttg tacaagcaa 
      gtcgaattg tacctgtgg taggaatcg agaaccggt 
      ctatgggga tgttagttc gtttacagt gaatagtcc 
      cgaattgcg aagaaaccg aagattttt gtaactgtg 
      ttcaaatta cgtttagtt tttgtacat agcagggag 
      tgccagttt gttttttgt atataatat acaagtgta 
      cttattttt ttacaaaaa taaattcat ttttgacca
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