Status
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Gene information
General information
TC009789 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8171870..8178834
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009789
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08730

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009789 RA
      MQSEPGRYI NSVSDLLYS TDEIEFLLD DIRDLEPTS 
      CKPEDIQDF EIAGSRTGA LSADCAGGC ECESPLGTV 
      NSWDSHSHY RRRVASPDL SLYSGVIVY CDHTHLKTP 
      TGIVRILLL VTTIACLTC LCTSGTVKV GLFMLPLVG 
      RLRLMMFVT LLSIFITCL LLFLDISHV VYLFPFNWG 
      KVNAYLYVS LSVLFLVAS SLIFHTIFL SEAFYWVPR 
      WTHHQLLIT GCLGYLCCF QSAILSLIQ KCSMGHYQP 
      VAEDPSLNL QERQTSLQD SPEHKANQT THNYKPIAS 
      YPVASTSRQ DASNYLDDV QPCSSKSSD PYRVA
    CDS sequence
    • >TC009789 RA
      atgcagtct gagccgggc cgatacatc aattcagta 
      tccgatctg ctttacagc accgacgaa attgaattt 
      ctgctcgac gacattaga gacctggag ccgacaagt 
      tgcaagcct gaagatatt caggacttt gagatagcg 
      ggcagcagg acgggggcc ctgagcgcg gattgtgct 
      ggaggttgc gagtgcgaa tcacctctg ggcaccgtt 
      aactcatgg gactcgcac tctcactac cgccgacgg 
      gtcgcctcg cccgatctg tccctatac tctggggtt 
      atcgtctac tgcgaccac actcatctc aagacgccc 
      acaggaata gtccgaata cttctgctg gtgaccaca 
      atcgcctgt ctcacatgc ctctgtacg tcaggaaca 
      gtaaaagta ggcctattt atgcttccg ctggtcgga 
      cgattacgg ctgatgatg ttcgtaacg ttactgagt 
      attttcatc acctgtctt ctcctcttt cttgatatt 
      tctcatgtc gtttatttg ttcccattt aactggggc 
      aaagtgaac gcttatttg tacgtcagc cttagtgtt 
      ctgttcctc gtagcgtcg tcgttaatt ttccacacg 
      attttctta tcagaggcc ttttactgg gtcccgagg 
      tggacacac catcagcta ctgatcacg gggtgtctg 
      gggtacctg tgttgcttc cagtcagca attttgtcg 
      ctgattcag aagtgtagc atggggcac taccagccg 
      gtggccgaa gaccccagc ctgaacctg caggagcgc 
      cagacctcg ctgcaagac tcgcccgag cataaggcg 
      aaccaaaca acgcacaat tacaagcct atagccagc 
      taccccgtg gcgtcgacg tcgcgtcaa gacgcctcg 
      aactacctc gacgacgtc cagccgtgt tcgtcgaaa 
      agttccgac ccgtacagg gtggcatga
    mRNA sequence
    • >TC009789 RA
      ccttccaca aaaaatgta tttaggtcg cgctaatct 
      caaaatttg attggctgc gagagggtg cctacccta 
      atcgaatct cgcgtccga tgaataaaa aaggggtgc 
      caacttgtg catttgacg tgtcgttgg tactcgacc 
      gcttcacaa tttttcaat aattaaata attcccgtt 
      taatgatgc ggagtgtag cagtttggc cccgttcgc 
      ccccatccc gatcgtcat gactcccgt ccccgcaca 
      cccttgaag cgtcgaaag tggggtagt ggtagcgcg 
      tcgggccgc atttagtaa acatggcgc ggtgtttgc 
      ggcttgttt ggggtgacc gccttggat ggtaaacgc 
      cgttcgcgc aggtcagta gtgcgtttt cgggtggag 
      aagctggcg agtcatgtg tccgcattg aggagttcg 
      cagggccgc gcggtatgg attggtgtg gggcgaagc 
      ctcgcgtct agtggcgga gtgacatgc agtctgagc 
      cgggccgat acatcaatt cagtatccg atctgcttt 
      acagcaccg acgaaattg aatttctgc tcgacgaca 
      ttagagacc tggagccga caagttgca agcctgaag 
      atattcagg actttgaga tagcgggca gcaggacgg 
      gggccctga gcgcggatt gtgctggag gttgcgagt 
      gcgaatcac ctctgggca ccgttaact catgggact 
      cgcactctc actaccgcc gacgggtcg cctcgcccg 
      atctgtccc tatactctg gggttatcg tctactgcg 
      accacactc atctcaaga cgcccacag gaatagtcc 
      gaatacttc tgctggtga ccacaatcg cctgtctca 
      catgcctct gtacgtcag gaacagtaa aagtaggcc 
      tatttatgc ttccgctgg tcggacgat tacggctga 
      tgatgttcg taacgttac tgagtattt tcatcacct 
      gtcttctcc tctttcttg atatttctc atgtcgttt 
      atttgttcc catttaact ggggcaaag tgaacgctt 
      atttgtacg tcagcctta gtgttctgt tcctcgtag 
      cgtcgtcgt taattttcc acacgattt tcttatcag 
      aggcctttt actgggtcc cgaggtgga cacaccatc 
      agctactga tcacggggt gtctggggt acctgtgtt 
      gcttccagt cagcaattt tgtcgctga ttcagaagt 
      gtagcatgg ggcactacc agccggtgg ccgaagacc 
      ccagcctga acctgcagg agcgccaga cctcgctgc 
      aagactcgc ccgagcata aggcgaacc aaacaacgc 
      acaattaca agcctatag ccagctacc ccgtggcgt 
      cgacgtcgc gtcaagacg cctcgaact acctcgacg 
      acgtccagc cgtgttcgt cgaaaagtt ccgacccgt 
      acagggtgg catgatatt taacttaaa actcttcta 
      aatgccttc tgtgtttat cgtgcagtg aatgtgcag 
      attcgacaa gtgtggata gatagtcaa acaccagtt 
      aagaaatat atcattgtt tcgatttgt tttgaattt 
      ttacacggc atgtataaa aatgtatag acaaataaa 
      tatattcca aattattgc aatatttgc aaagttagt 
      atacggatc ctgcagcaa gttcactcg ttcagaata 
      ggcaagaaa agaagttta ttgtaaata gttttttag 
      cgtttttca aatttagtg tttatattc tgtacagta 
      ttaacttca cgagtttca ttttagact agacagaac 
      tttttcata atataagaa acaaagtgt tcaattcgt 
      tttattgtg tgtatactt acctaagca caagtccat 
      gttttttgt attgtatgt gacggattt atgtttggt 
      tgtagcctc taacatggt gctgaaaaa cattccaga 
      gatgttttt ccctaacat gattctaaa cgaccccat 
      ttcccatca gatatttaa tatttcaag ccggaaatt 
      gcgttttaa ttccggaaa tcgagtccg accctcccg 
      agacagcac ttcaatcac aaattgttt ttctatcaa 
      ttaaaataa ttagtcgtt tcgaatcat tgcaaaaat 
      tctagtccg tgcccatat ttacttcct ccaaatgga 
      gtaagtatg acttgaccc tcattaatt tcagtcaaa 
      actgccaaa gttggaaac tagccaaaa atagagcga 
      gtttttact ttaggagac aatcagggc atcaaaact 
      gatttattt tgagtaatg taaaaattg tgttctcgt 
      gtgtatttt acaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY