Status
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Gene information
General information
TC009085 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7156567..7167095
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009085
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08721

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009085 RA
      MASGDKKSP QLSKIKVKV GNSTVTASD SDASVETNS 
      LSADSTTEQ NNVTQTEKR RKKARRGSE WEIMEGLKD 
      GQRFESKPN PFSGFLHKK RKWPLKGWH KRYFLLEKG 
      ILVYGKGPN EISKGKIHG TVDIGLSVI TTKSKRRRI 
      DIDAEEFIY HLKAKSEDA FTSWVQQLT AHRLYRQHI 
      LTYGTNVGT LFKPADGLH SIPRTPEIV SRDGSLTRG 
      LQPPSSGGR LSLWLQESL SSLEQFQRD TTNVEQNVT 
      KLSRLLQQI EASAILGTE VVEALSPNI KKDRRKFGL 
      KKKKSSKGG SVDLTIQFS AANKSSGTD ADNTSPLSA 
      NSFSALSTS PQQLGVNTV ASLPVPNAS TTPTPDSLS 
      NVDVISLST ENQLREDFL SLAKTVLGS LKTLTFGLS 
      TERERLKSA LELEGTVNV NQSVVNLRN NLNQVLQQN 
      TELKSRLLR IHEASDLTD LSSLEQLSE NHRPYNASL 
      SYSSSCVSA TEFFDAEDL PTEKTILDT EDPDIQEEE 
      SEVRTRSES SSEAGSLSS GEGSISSES ELGTELNTV 
      NNSLDLAGS QSLTGRRTV LPAPRPHTE GLSLWNLLS 
      RNIGKDLSQ ISMPVALNE PLNVLQRLC EELEYSELL 
      DKAASLDDP YERMVQIAA FAVSAYAST LTRAGNKPF 
      NPLLGETYE CTREDKGFK FLAEQVSHH PPISTCYAK 
      SNNFTFWQD ARVKTKFWG KSMEFQPLG SVHVLLPRT 
      GDLYTWNKV TTCVHNLFS GQRWVDQYG ELRVSNGRI 
      TCKLTFNKA SYWSAKRHE VVGAVFDES GKTVHRLFG 
      KWSESLYCG VPPSARCIW RAGNLPPNH ERYYGFTRF 
      AIELNELGP DAHLLPPTD TRFRPDQRA LEEGDLNTA 
      ETLKLQLES AQRDRRKRR EELNIRYEP RWFSNQKDD 
      MWEYNGKYW EMRKNPGFA NMQFESLW
    • >TC009085 RB
      MYLHLSDLA VRHDVFWKD RNDCRKSRK IMASGDKKS 
      PQLSKIKVK VGNSTVTAS DSDASVETN SLSADSTTE 
      QNNVTQTEK RRKKARRGS EWEIMEGLK DGQRFESKP 
      NPFSGFLHK KRKWPLKGW HKRYFLLEK GILVYGKGP 
      NEISKGKIH GTVDIGLSV ITTKSKRRR IDIDAEEFI 
      YHLKAKSED AFTSWVQQL TAHRLYRQH ILTYGTNVG 
      TLFKPADGL HSIPRTPEI VSRDGSLTR GLQPPSSGG 
      RLSLWLQES LSSLEQFQR DTTNVEQNV TKLSRLLQQ 
      IEASAILGT EVVEALSPN IKKDRRKFG LKKKKSSKG 
      GSVDLTIQF SAANKSSGT DADNTSPLS ANSFSALST 
      SPQQLGVNT VASLPVPNA STTPTPDSL SNVDVISLS 
      TENQLREDF LSLAKTVLG SLKTLTFGL STERERLKS 
      ALELEGTVN VNQSVVNLR NNLNQVLQQ NTELKSRLL 
      RIHEASDLT DLSSLEQLS ENHRPYNAS LSYSSSCVS 
      ATEFFDAED LPTEKTILD TEDPDIQEE ESEVRTRSE 
      SSSEAGSLS SGEGSISSE SELGTELNT VNNSLDLAG 
      SQSLTGRRT VLPAPRPHT EGLSLWNLL SRNIGKDLS 
      QISMPVALN EPLNVLQRL CEELEYSEL LDKAASLDD 
      PYERMVQIA AFAVSAYAS TLTRAGNKP FNPLLGETY 
      ECTREDKGF KFLAEQVSH HPPISTCYA KSNNFTFWQ 
      DARVKTKFW GKSMEFQPL GSVHVLLPR TGDLYTWNK 
      VTTCVHNLF SGQRWVDQY GELRVSNGR ITCKLTFNK 
      ASYWSAKRH EVVGAVFDE SGKTVHRLF GKWSESLYC 
      GVPPSARCI WRAGNLPPN HERYYGFTR FAIELNELG 
      PDAHLLPPT DTRFRPDQR ALEEGDLNT AETLKLQLE 
      SAQRDRRKR REELNIRYE PRWFSNQKD DMWEYNGKY 
      WEMRKNPGF ANMQFESLW
    CDS sequence
    • >TC009085 RA
      atggccagc ggcgacaag aagagtcca caactgagc 
      aaaatcaaa gtcaaggtc ggaaatagt acggtgaca 
      gccagtgat tcggacgct agcgtggaa actaatagt 
      ttgagtgcg gatagtacg acggaacag aataatgtc 
      acgcaaact gaaaagcga cgaaagaaa gccaggagg 
      gggtcggaa tgggaaatt atggaaggg ttgaaagat 
      gggcaaagg tttgaaagt aagccgaac ccgtttagt 
      ggatttttg cataagaag aggaagtgg cctttgaag 
      gggtggcat aagcgttat tttttactc gaaaagggt 
      atattagtt tatggaaag ggccctaat gaaataagt 
      aaaggaaaa atccacggc accgtcgat attggtttg 
      tctgtgatc actacgaaa agcaagagg cgacgtata 
      gatatagac gcggaagaa tttatttat catttgaaa 
      gcgaaaagt gaggatgct tttacgagc tgggtacaa 
      caactcact gctcaccgg ttgtacaga caacatatt 
      ttgacgtat ggaacgaac gttgggacg ctttttaaa 
      ccagccgat gggttgcat agtataccg aggactcct 
      gaaatagtg agtcgcgat ggcagcctt actcgaggc 
      ttgcaaccg ccatctagt ggcggccgg ctcagttta 
      tggctccag gaatcactc tcttccctt gaacaattt 
      caaagagat acaacaaat gtcgaacaa aacgtaacg 
      aaattatcg cgactcttg caacaaatc gaagctagt 
      gctattttg gggactgag gtggttgag gcactctcg 
      cccaatatt aaaaaagat aggaggaag tttggtttg 
      aagaagaaa aagtcgtcg aaaggtggc agtgttgat 
      ctaacaata cagttcagt gcagctaat aaatcatca 
      ggaaccgat gctgacaac acttcgcca ctatctgcg 
      aattctttt tcagctctt tcgacctct cctcagcaa 
      cttggagtc aataccgtg gccagtttg ccagtgcct 
      aatgcttca acaactcca actcctgat agtcttagt 
      aatgtagat gttatctcc ttgtcaacg gagaatcag 
      cttagggaa gatttcttg tcgttggca aaaactgtt 
      cttggtagt cttaaaacg ttaactttt ggcttaagt 
      accgaacgt gagcgtctt aagtcagct ctagagtta 
      gaaggaacc gtcaatgtc aatcaaagt gtagttaat 
      ttaagaaat aatctaaac caagttctc cagcaaaac 
      accgaactg aaaagtcgt ctgttgcga atccacgaa 
      gcttcagac ttgaccgac ttgtcttcg ttagagcag 
      ctctcggaa aaccatcgt ccctataac gcttcgttg 
      agctatagt tcctcgtgt gtgagtgca accgaattt 
      ttcgatgcc gaagattta ccgactgaa aaaaccatc 
      ctggatacc gaagatcca gacatacaa gaagaggaa 
      tctgaggtg cgtacacgt tctgaaagt agttcggag 
      gcgggctcg ctctccagc ggcgaagga agcatcagt 
      tcagagtct gaactcggc acggagcta aacacagtc 
      aataattcg ctagatttg gcagggtcc cagagcctg 
      accggccgc cggacggtc cttcctgca cctcgtccc 
      cacaccgaa ggcctctcc ctatggaac ctcttgagc 
      agaaacatc ggcaaagac ctgtcccag ataagtatg 
      cctgtggcc ctcaacgaa cccctcaat gtcctccag 
      aggctgtgc gaagagctc gagtattcc gaattgttg 
      gacaaagcg gcttctttg gacgacccg tacgaacgt 
      atggtccaa atagccgcg tttgccgtt tccgcctat 
      gccagtact cttaccaga gccggaaac aagcctttc 
      aatcctcta ttaggggag acgtatgag tgtactcga 
      gaggataaa gggtttaag tttttggcg gaacaagtg 
      tcgcatcat ccccccatt agcacttgt tatgcgaaa 
      agtaataat ttcacgttt tggcaagac gcaagagtt 
      aaaacaaaa ttctggggg aaaagtatg gagtttcaa 
      ccgctgggg agtgtgcat gtgctgttg ccccgaacc 
      ggggattta tacacgtgg aataaagtc acaacttgt 
      gtccataat ttattcagc gggcaaaga tgggtcgat 
      cagtatgga gagttgaga gttagtaat gggcgaatc 
      acgtgcaag ttgactttc aacaaggcc agctattgg 
      tcggcgaaa agacacgag gtcgtgggg gccgtgttc 
      gacgagtcg ggcaagacg gtgcaccgt ttgtttggt 
      aagtggtca gagtcgttg tactgtggg gtcccccca 
      tcggctcgg tgcatttgg agggccggg aatttgccc 
      ccgaaccac gagcgttac tacggattt acccgattc 
      gcaatcgag ttgaatgaa ttgggcccc gatgcgcat 
      ttgttgccg cctactgat actcgtttc cggcctgac 
      cagagggcg ctggaggag ggtgatttg aatacggcc 
      gaaaccctg aaattacag ttggaaagt gcgcagaga 
      gaccgtagg aagaggagg gaggagttg aatatcagg 
      tatgagccg cgatggttc tcgaaccag aaggatgat 
      atgtgggag tacaatggg aagtactgg gagatgcgg 
      aaaaatccc gggtttgcg aatatgcag ttcgagtcg 
      ttgtggtga
    • >TC009085 RB
      atgtatttg catctctct gatcttgca gttagacat 
      gacgttttt tggaaagat agaaacgat tgtcgcaag 
      agtcgcaag attatggcc agcggcgac aagaagagt 
      ccacaactg agcaaaatc aaagtcaag gtcggaaat 
      agtacggtg acagccagt gattcggac gctagcgtg 
      gaaactaat agtttgagt gcggatagt acgacggaa 
      cagaataat gtcacgcaa actgaaaag cgacgaaag 
      aaagccagg agggggtcg gaatgggaa attatggaa 
      gggttgaaa gatgggcaa aggtttgaa agtaagccg 
      aacccgttt agtggattt ttgcataag aagaggaag 
      tggcctttg aaggggtgg cataagcgt tatttttta 
      ctcgaaaag ggtatatta gtttatgga aagggccct 
      aatgaaata agtaaagga aaaatccac ggcaccgtc 
      gatattggt ttgtctgtg atcactacg aaaagcaag 
      aggcgacgt atagatata gacgcggaa gaatttatt 
      tatcatttg aaagcgaaa agtgaggat gcttttacg 
      agctgggta caacaactc actgctcac cggttgtac 
      agacaacat attttgacg tatggaacg aacgttggg 
      acgcttttt aaaccagcc gatgggttg catagtata 
      ccgaggact cctgaaata gtgagtcgc gatggcagc 
      cttactcga ggcttgcaa ccgccatct agtggcggc 
      cggctcagt ttatggctc caggaatca ctctcttcc 
      cttgaacaa tttcaaaga gatacaaca aatgtcgaa 
      caaaacgta acgaaatta tcgcgactc ttgcaacaa 
      atcgaagct agtgctatt ttggggact gaggtggtt 
      gaggcactc tcgcccaat attaaaaaa gataggagg 
      aagtttggt ttgaagaag aaaaagtcg tcgaaaggt 
      ggcagtgtt gatctaaca atacagttc agtgcagct 
      aataaatca tcaggaacc gatgctgac aacacttcg 
      ccactatct gcgaattct ttttcagct ctttcgacc 
      tctcctcag caacttgga gtcaatacc gtggccagt 
      ttgccagtg cctaatgct tcaacaact ccaactcct 
      gatagtctt agtaatgta gatgttatc tccttgtca 
      acggagaat cagcttagg gaagatttc ttgtcgttg 
      gcaaaaact gttcttggt agtcttaaa acgttaact 
      tttggctta agtaccgaa cgtgagcgt cttaagtca 
      gctctagag ttagaagga accgtcaat gtcaatcaa 
      agtgtagtt aatttaaga aataatcta aaccaagtt 
      ctccagcaa aacaccgaa ctgaaaagt cgtctgttg 
      cgaatccac gaagcttca gacttgacc gacttgtct 
      tcgttagag cagctctcg gaaaaccat cgtccctat 
      aacgcttcg ttgagctat agttcctcg tgtgtgagt 
      gcaaccgaa tttttcgat gccgaagat ttaccgact 
      gaaaaaacc atcctggat accgaagat ccagacata 
      caagaagag gaatctgag gtgcgtaca cgttctgaa 
      agtagttcg gaggcgggc tcgctctcc agcggcgaa 
      ggaagcatc agttcagag tctgaactc ggcacggag 
      ctaaacaca gtcaataat tcgctagat ttggcaggg 
      tcccagagc ctgaccggc cgccggacg gtccttcct 
      gcacctcgt ccccacacc gaaggcctc tccctatgg 
      aacctcttg agcagaaac atcggcaaa gacctgtcc 
      cagataagt atgcctgtg gccctcaac gaacccctc 
      aatgtcctc cagaggctg tgcgaagag ctcgagtat 
      tccgaattg ttggacaaa gcggcttct ttggacgac 
      ccgtacgaa cgtatggtc caaatagcc gcgtttgcc 
      gtttccgcc tatgccagt actcttacc agagccgga 
      aacaagcct ttcaatcct ctattaggg gagacgtat 
      gagtgtact cgagaggat aaagggttt aagtttttg 
      gcggaacaa gtgtcgcat catcccccc attagcact 
      tgttatgcg aaaagtaat aatttcacg ttttggcaa 
      gacgcaaga gttaaaaca aaattctgg gggaaaagt 
      atggagttt caaccgctg gggagtgtg catgtgctg 
      ttgccccga accggggat ttatacacg tggaataaa 
      gtcacaact tgtgtccat aatttattc agcgggcaa 
      agatgggtc gatcagtat ggagagttg agagttagt 
      aatgggcga atcacgtgc aagttgact ttcaacaag 
      gccagctat tggtcggcg aaaagacac gaggtcgtg 
      ggggccgtg ttcgacgag tcgggcaag acggtgcac 
      cgtttgttt ggtaagtgg tcagagtcg ttgtactgt 
      ggggtcccc ccatcggct cggtgcatt tggagggcc 
      gggaatttg cccccgaac cacgagcgt tactacgga 
      tttacccga ttcgcaatc gagttgaat gaattgggc 
      cccgatgcg catttgttg ccgcctact gatactcgt 
      ttccggcct gaccagagg gcgctggag gagggtgat 
      ttgaatacg gccgaaacc ctgaaatta cagttggaa 
      agtgcgcag agagaccgt aggaagagg agggaggag 
      ttgaatatc aggtatgag ccgcgatgg ttctcgaac 
      cagaaggat gatatgtgg gagtacaat gggaagtac 
      tgggagatg cggaaaaat cccgggttt gcgaatatg 
      cagttcgag tcgttgtgg tga
    mRNA sequence
    • >TC009085 RA
      tagcaatgt ctagtaaaa tcatcctgt ataaaaaaa 
      gtattgtca acgtagtgc ttgttgtgt tttgattcg 
      aggagtgca agtaaatag cagtgcaaa ttgtagtgt 
      aaattatga taaattcag tgcaacatt gtaaaaagc 
      cttctgtga caaggtcca tggaatata attggtgtt 
      attggattt aataagtgt cgcaagagt cgcaagatt 
      atggccagc ggcgacaag aagagtcca caactgagc 
      aaaatcaaa gtcaaggtc ggaaatagt acggtgaca 
      gccagtgat tcggacgct agcgtggaa actaatagt 
      ttgagtgcg gatagtacg acggaacag aataatgtc 
      acgcaaact gaaaagcga cgaaagaaa gccaggagg 
      gggtcggaa tgggaaatt atggaaggg ttgaaagat 
      gggcaaagg tttgaaagt aagccgaac ccgtttagt 
      ggatttttg cataagaag aggaagtgg cctttgaag 
      gggtggcat aagcgttat tttttactc gaaaagggt 
      atattagtt tatggaaag ggccctaat gaaataagt 
      aaaggaaaa atccacggc accgtcgat attggtttg 
      tctgtgatc actacgaaa agcaagagg cgacgtata 
      gatatagac gcggaagaa tttatttat catttgaaa 
      gcgaaaagt gaggatgct tttacgagc tgggtacaa 
      caactcact gctcaccgg ttgtacaga caacatatt 
      ttgacgtat ggaacgaac gttgggacg ctttttaaa 
      ccagccgat gggttgcat agtataccg aggactcct 
      gaaatagtg agtcgcgat ggcagcctt actcgaggc 
      ttgcaaccg ccatctagt ggcggccgg ctcagttta 
      tggctccag gaatcactc tcttccctt gaacaattt 
      caaagagat acaacaaat gtcgaacaa aacgtaacg 
      aaattatcg cgactcttg caacaaatc gaagctagt 
      gctattttg gggactgag gtggttgag gcactctcg 
      cccaatatt aaaaaagat aggaggaag tttggtttg 
      aagaagaaa aagtcgtcg aaaggtggc agtgttgat 
      ctaacaata cagttcagt gcagctaat aaatcatca 
      ggaaccgat gctgacaac acttcgcca ctatctgcg 
      aattctttt tcagctctt tcgacctct cctcagcaa 
      cttggagtc aataccgtg gccagtttg ccagtgcct 
      aatgcttca acaactcca actcctgat agtcttagt 
      aatgtagat gttatctcc ttgtcaacg gagaatcag 
      cttagggaa gatttcttg tcgttggca aaaactgtt 
      cttggtagt cttaaaacg ttaactttt ggcttaagt 
      accgaacgt gagcgtctt aagtcagct ctagagtta 
      gaaggaacc gtcaatgtc aatcaaagt gtagttaat 
      ttaagaaat aatctaaac caagttctc cagcaaaac 
      accgaactg aaaagtcgt ctgttgcga atccacgaa 
      gcttcagac ttgaccgac ttgtcttcg ttagagcag 
      ctctcggaa aaccatcgt ccctataac gcttcgttg 
      agctatagt tcctcgtgt gtgagtgca accgaattt 
      ttcgatgcc gaagattta ccgactgaa aaaaccatc 
      ctggatacc gaagatcca gacatacaa gaagaggaa 
      tctgaggtg cgtacacgt tctgaaagt agttcggag 
      gcgggctcg ctctccagc ggcgaagga agcatcagt 
      tcagagtct gaactcggc acggagcta aacacagtc 
      aataattcg ctagatttg gcagggtcc cagagcctg 
      accggccgc cggacggtc cttcctgca cctcgtccc 
      cacaccgaa ggcctctcc ctatggaac ctcttgagc 
      agaaacatc ggcaaagac ctgtcccag ataagtatg 
      cctgtggcc ctcaacgaa cccctcaat gtcctccag 
      aggctgtgc gaagagctc gagtattcc gaattgttg 
      gacaaagcg gcttctttg gacgacccg tacgaacgt 
      atggtccaa atagccgcg tttgccgtt tccgcctat 
      gccagtact cttaccaga gccggaaac aagcctttc 
      aatcctcta ttaggggag acgtatgag tgtactcga 
      gaggataaa gggtttaag tttttggcg gaacaagtg 
      tcgcatcat ccccccatt agcacttgt tatgcgaaa 
      agtaataat ttcacgttt tggcaagac gcaagagtt 
      aaaacaaaa ttctggggg aaaagtatg gagtttcaa 
      ccgctgggg agtgtgcat gtgctgttg ccccgaacc 
      ggggattta tacacgtgg aataaagtc acaacttgt 
      gtccataat ttattcagc gggcaaaga tgggtcgat 
      cagtatgga gagttgaga gttagtaat gggcgaatc 
      acgtgcaag ttgactttc aacaaggcc agctattgg 
      tcggcgaaa agacacgag gtcgtgggg gccgtgttc 
      gacgagtcg ggcaagacg gtgcaccgt ttgtttggt 
      aagtggtca gagtcgttg tactgtggg gtcccccca 
      tcggctcgg tgcatttgg agggccggg aatttgccc 
      ccgaaccac gagcgttac tacggattt acccgattc 
      gcaatcgag ttgaatgaa ttgggcccc gatgcgcat 
      ttgttgccg cctactgat actcgtttc cggcctgac 
      cagagggcg ctggaggag ggtgatttg aatacggcc 
      gaaaccctg aaattacag ttggaaagt gcgcagaga 
      gaccgtagg aagaggagg gaggagttg aatatcagg 
      tatgagccg cgatggttc tcgaaccag aaggatgat 
      atgtgggag tacaatggg aagtactgg gagatgcgg 
      aaaaatccc gggtttgcg aatatgcag ttcgagtcg 
      ttgtggtga ctggcaata cgcaatttg taggctaaa 
      ccagtgatt tgcgtgcaa ttatagtga ttttagtat 
      ttaaattgt ttccttatg aatgccatg cttgtataa 
      accggtcgt ttcagagtt actatttta tttttaagc 
      aataagaga tttaatgat tataatgga atgaagtgt 
      agtgatata tccattagt ttttaggta gtggcattt 
      tagttacgt agctaatgc aaaaaatca attgtgtat 
      tggtaagaa aataaaaat tccactttg aactttcct 
      atctagtca ctaattatt gttcaataa gattaaata 
      atgtattgt tattgttgt aacttaata tttattttt 
      atgttaaga aatgtaatt tttctttta ttgtttttg 
      atggaaaac atataacag ctacattgt tcataaatt 
      aaagtgtct ttttcggat tcttgtaga attagttgg 
      atttttatg tcaatgaag ggtgacaat aatcactac 
      aacgccaat tgtttaaag gaagcttca tttttaaac 
      atgcgcatt gctttcaca ggacctaac tagacgtta 
      tcattcatt ttaaattct gttatttgt aaaatgctc 
      ttttaaagg tgaaggtta aataaaaat gttaaacat 
      ta
    • >TC009085 RB
      tagcaatgt ctagtaaaa tcatcctgt ataaaaaaa 
      gtattgtca acgtagtgc ttgttgtgt tttgattcg 
      aggagtgca agtaaatag cagtgcaaa ttgtagtgt 
      aaattatga taaattcag tgcaacatt gtaaaaagc 
      cttctgtga caaggtcca tggaatata attggtgtt 
      attggattt aataagaaa cgaaaaatt tcgaatgtc 
      ttgccaatg tatttgcat ctctctgat cttgcagtt 
      agacatgac gttttttgg aaagataga aacgattgt 
      cgcaagagt cgcaagatt atggccagc ggcgacaag 
      aagagtcca caactgagc aaaatcaaa gtcaaggtc 
      ggaaatagt acggtgaca gccagtgat tcggacgct 
      agcgtggaa actaatagt ttgagtgcg gatagtacg 
      acggaacag aataatgtc acgcaaact gaaaagcga 
      cgaaagaaa gccaggagg gggtcggaa tgggaaatt 
      atggaaggg ttgaaagat gggcaaagg tttgaaagt 
      aagccgaac ccgtttagt ggatttttg cataagaag 
      aggaagtgg cctttgaag gggtggcat aagcgttat 
      tttttactc gaaaagggt atattagtt tatggaaag 
      ggccctaat gaaataagt aaaggaaaa atccacggc 
      accgtcgat attggtttg tctgtgatc actacgaaa 
      agcaagagg cgacgtata gatatagac gcggaagaa 
      tttatttat catttgaaa gcgaaaagt gaggatgct 
      tttacgagc tgggtacaa caactcact gctcaccgg 
      ttgtacaga caacatatt ttgacgtat ggaacgaac 
      gttgggacg ctttttaaa ccagccgat gggttgcat 
      agtataccg aggactcct gaaatagtg agtcgcgat 
      ggcagcctt actcgaggc ttgcaaccg ccatctagt 
      ggcggccgg ctcagttta tggctccag gaatcactc 
      tcttccctt gaacaattt caaagagat acaacaaat 
      gtcgaacaa aacgtaacg aaattatcg cgactcttg 
      caacaaatc gaagctagt gctattttg gggactgag 
      gtggttgag gcactctcg cccaatatt aaaaaagat 
      aggaggaag tttggtttg aagaagaaa aagtcgtcg 
      aaaggtggc agtgttgat ctaacaata cagttcagt 
      gcagctaat aaatcatca ggaaccgat gctgacaac 
      acttcgcca ctatctgcg aattctttt tcagctctt 
      tcgacctct cctcagcaa cttggagtc aataccgtg 
      gccagtttg ccagtgcct aatgcttca acaactcca 
      actcctgat agtcttagt aatgtagat gttatctcc 
      ttgtcaacg gagaatcag cttagggaa gatttcttg 
      tcgttggca aaaactgtt cttggtagt cttaaaacg 
      ttaactttt ggcttaagt accgaacgt gagcgtctt 
      aagtcagct ctagagtta gaaggaacc gtcaatgtc 
      aatcaaagt gtagttaat ttaagaaat aatctaaac 
      caagttctc cagcaaaac accgaactg aaaagtcgt 
      ctgttgcga atccacgaa gcttcagac ttgaccgac 
      ttgtcttcg ttagagcag ctctcggaa aaccatcgt 
      ccctataac gcttcgttg agctatagt tcctcgtgt 
      gtgagtgca accgaattt ttcgatgcc gaagattta 
      ccgactgaa aaaaccatc ctggatacc gaagatcca 
      gacatacaa gaagaggaa tctgaggtg cgtacacgt 
      tctgaaagt agttcggag gcgggctcg ctctccagc 
      ggcgaagga agcatcagt tcagagtct gaactcggc 
      acggagcta aacacagtc aataattcg ctagatttg 
      gcagggtcc cagagcctg accggccgc cggacggtc 
      cttcctgca cctcgtccc cacaccgaa ggcctctcc 
      ctatggaac ctcttgagc agaaacatc ggcaaagac 
      ctgtcccag ataagtatg cctgtggcc ctcaacgaa 
      cccctcaat gtcctccag aggctgtgc gaagagctc 
      gagtattcc gaattgttg gacaaagcg gcttctttg 
      gacgacccg tacgaacgt atggtccaa atagccgcg 
      tttgccgtt tccgcctat gccagtact cttaccaga 
      gccggaaac aagcctttc aatcctcta ttaggggag 
      acgtatgag tgtactcga gaggataaa gggtttaag 
      tttttggcg gaacaagtg tcgcatcat ccccccatt 
      agcacttgt tatgcgaaa agtaataat ttcacgttt 
      tggcaagac gcaagagtt aaaacaaaa ttctggggg 
      aaaagtatg gagtttcaa ccgctgggg agtgtgcat 
      gtgctgttg ccccgaacc ggggattta tacacgtgg 
      aataaagtc acaacttgt gtccataat ttattcagc 
      gggcaaaga tgggtcgat cagtatgga gagttgaga 
      gttagtaat gggcgaatc acgtgcaag ttgactttc 
      aacaaggcc agctattgg tcggcgaaa agacacgag 
      gtcgtgggg gccgtgttc gacgagtcg ggcaagacg 
      gtgcaccgt ttgtttggt aagtggtca gagtcgttg 
      tactgtggg gtcccccca tcggctcgg tgcatttgg 
      agggccggg aatttgccc ccgaaccac gagcgttac 
      tacggattt acccgattc gcaatcgag ttgaatgaa 
      ttgggcccc gatgcgcat ttgttgccg cctactgat 
      actcgtttc cggcctgac cagagggcg ctggaggag 
      ggtgatttg aatacggcc gaaaccctg aaattacag 
      ttggaaagt gcgcagaga gaccgtagg aagaggagg 
      gaggagttg aatatcagg tatgagccg cgatggttc 
      tcgaaccag aaggatgat atgtgggag tacaatggg 
      aagtactgg gagatgcgg aaaaatccc gggtttgcg 
      aatatgcag ttcgagtcg ttgtggtga ctggcaata 
      cgcaatttg taggctaaa ccagtgatt tgcgtgcaa 
      ttatagtga ttttagtat ttaaattgt ttccttatg 
      aatgccatg cttgtataa accggtcgt ttcagagtt 
      actatttta tttttaagc aataagaga tttaatgat 
      tataatgga atgaagtgt agtgatata tccattagt 
      ttttaggta gtggcattt tagttacgt agctaatgc 
      aaaaaatca attgtgtat tggtaagaa aataaaaat 
      tccactttg aactttcct atctagtca ctaattatt 
      gttcaataa gattaaata atgtattgt tattgttgt 
      aacttaata tttattttt atgttaaga aatgtaatt 
      tttctttta ttgtttttg atggaaaac atataacag 
      ctacattgt tcataaatt aaagtgtct ttttcggat 
      tcttgtaga attagttgg atttttatg tcaatgaag 
      ggtgacaat aatcactac aacgccaat tgtttaaag 
      gaagcttca tttttaaac atgcgcatt gctttcaca 
      ggacctaac tagacgtta tcattcatt ttaaattct 
      gttatttgt aaaatgctc ttttaaagg tgaaggtta 
      aataaaaat gttaaacat ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY