Status
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Gene information
General information
TC033673
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016384,TC016386
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6750458..6783030
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033673
    iBeetle screen iB_08719

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033673 RA
      MEQNARRRG CFGDVVVCL RRAALILEP SLSAAWRRS 
      AAASRADAA GPAGLPPPP DPSVSHAHG ARTLIRCKG 
      FYDTLRGPE YVSPSLCGS GSYRRDRKY RRETLLRVS 
      TPPVSQITI FSYAPWALF RSGVVSGQS EIRVAVASK 
      TRRPVPSHP DSPSSQLIL LKSVPVRTA TAAGRSHGR 
      DRTRSFADP CTNYTPIAA SVGGHCALP CLPANRPLA 
      LDTSGVYPS STQTKFTNF NRQKIQTKP KTRVIMSGF 
      KESQETSLT PLCCFLPNR FYSPHTTFV RKSGQPHEL 
      ACVLCSLLI GLGYDAYVV FGYAVRDIT LRIMSRVDS 
      PYPPDEKQE DVRDEGEDN KKYTVKPPR DLRSKFLLM 
      MEQKKIDQI KAEQEREAE LERQREEEE ERPPPDDLE 
      GMRFHFWVL IRTEGRAID ENTFVEPST GYAHPVNSP 
      LYSGIESVW NHLNYWVNM QDCKEGLGT IDYNLHDVE 
      KWEHLLIGE PTQWRVTVE NLNMDDEEA ALAKIMEEK 
      HLDMPISWS MKISIPHKA LKQKYPEGM ISTWYKKTL 
      VEEYAPYIQ YDGLLTKIM RYKDFDCTE CITVEQIYE 
      NRQDKLKKI VYDLKSDLV TEYFGPGRE DAVIEHTYS 
      NKDTDSNKR IIKFNHKAR YDGLEKIEF EREHMVEYY 
      IDRDDLFYF REVFYARKG QPPPGFTES HRKLILRII 
      EKYERNHKV PASHDIATR EFAIVDRQI LLKYHFGEG 
      RVTCSTRNF NKPSIAESG EGMKFTPEL TWGYVAEIG 
      AKPPTQLEL FLLFQKMLK EEEKSLSRI REIEDQVAE 
      FLTLRDHEM AFPKLDVSL FNIEQNEEY RQGMLEKEK 
      QEQEYKERE IEDAGVEYL TPYLTLLGN PESVTPVEA 
      FKIKEKCLS DFKQLLLNR AICIQKQFE HWTQYLKDR 
      QQWYNINQD TVTEEDEIK YFQEVNDLT FLLQCYEIR 
      LARHRDLSF FRYEAVVRY LNAQLKPLI ETFG
    CDS sequence
    • >TC033673 RA
      atggagcaa aacgcacga cggagaggg tgcttcggc 
      gacgtcgtg gtgtgtttg aggagggca gcgctaatc 
      ctggagccg tccctgagc gcggcgtgg cgacggtcg 
      gccgcggct tcgagagca gatgccgcg ggccccgcg 
      ggcctcccg ccgcccccc gatccctcc gtcagtcac 
      gcacacggg gcgcgaaca ctcatccgt tgcaagggt 
      ttttacgac acgttgcgt ggcccggaa tatgttagt 
      ccgagcctg tgcggatca gggtcgtat cgaagggac 
      cggaaatac cgccgcgag accctcctc cgcgtctcc 
      acgccgccg gtttcgcaa ataacgatc ttctcctac 
      gcgccgtgg gctttgttc cggagcggt gttgtttct 
      ggtcaaagc gaaattcgg gtcgcagta gcaagcaaa 
      acccgtcgt cccgtcccg tcccatccc gactctccc 
      tcatcccaa ctcatcctc cttaaatcc gtccctgtc 
      cgaaccgcg acagctgcg ggtagatca cacggcagg 
      gacaggacg cggagcttc gcggacccg tgcactaac 
      tacacaccg atcgccgcg agcgtcggc ggccattgt 
      gctttgccc tgcttgccc gccaaccga cccttggcc 
      cttgacacc agtggcgta tacccaagc tcaacacaa 
      accaaattt accaatttc aaccgccaa aagatccaa 
      accaagccg aaaacaaga gttataatg tccggattt 
      aaagaatct caggaaact tctttgacc ccgttatgt 
      tgttttttg ccaaaccgt ttctactct cctcacaca 
      accttcgtc cgcaagtcc ggacagccc cacgagctt 
      gcttgtgtc ctttgttcc ctcctcata ggcctcggt 
      tacgatgcc tacgtggtc ttcggctac gcagtgcga 
      gatataacg ttacgaata atgagtcgt gtcgattct 
      ccatacccc cccgatgag aaacaagaa gatgtacgt 
      gacgaaggt gaagacaat aagaaatac accgttaag 
      ccccccaga gatttgcga agtaaattt ttattgatg 
      atggaacag aaaaaaatc gatcagatc aaagccgaa 
      caagaacgc gaagccgaa ctggaacgg cagagagaa 
      gaggaggag gaacggccg ccgccggac gatctggaa 
      gggatgagg ttccatttt tgggtcctg ataaggaca 
      gaaggtcgc gccatcgac gaaaatact ttcgttgag 
      ccgagtact ggatatgcg catccggtt aatagtccg 
      ttgtacagt ggcatcgaa agtgtttgg aatcatttg 
      aattattgg gtcaatatg caagactgc aaggaaggg 
      ttgggtaca atcgactac aatttgcac gatgttgaa 
      aaatgggaa catttgttg atcggagag ccgactcag 
      tggagagtc accgtcgaa aatttaaat atggatgac 
      gaagaagcc gctttagcg aaaataatg gaagaaaaa 
      catttagac atgccgata tcttggagc atgaaaata 
      tctataccg cacaaagct ctaaaacaa aaatacccc 
      gaaggcatg atcagtact tggtataaa aaaacacta 
      gtcgaagaa tacgcccct tatattcaa tatgacgga 
      cttttaacc aaaattatg agatataaa gactttgat 
      tgtacggaa tgtatcaca gttgagcag atttacgaa 
      aacagacaa gacaaattg aagaaaatt gtttatgat 
      ttaaaaagc gatctagtc acggagtat tttggccct 
      gggcgagaa gatgccgtg attgagcac acttactcg 
      aataaagac accgattca aataagcgg ataattaag 
      ttcaaccac aaggcacgc tacgacggc ttggaaaaa 
      attgaattc gagcgggaa cacatggtt gaatattac 
      atagaccgt gacgatttg ttttatttt cgcgaagtg 
      ttctacgcc cgcaaaggc caacccccg cctgggttc 
      accgaaagt caccgaaaa ttgattcta agaataatc 
      gagaaatac gaaaggaac cataaagtc cccgcgagt 
      cacgatatc gcgacccga gaattcgca atcgtcgat 
      agacagatt cttctaaag tatcatttc ggggaagga 
      agagtcact tgctcgacc aggaatttc aataagcct 
      tcgattgcg gaatcgggg gagggaatg aagttcaca 
      cctgaactc acctggggt tatgtcgcc gaaatcggg 
      gcgaagccc cccacacag ctggagttg ttcctttta 
      ttccagaaa atgttgaaa gaagaggag aaatccctg 
      tcgcggatc cgcgaaatc gaggaccag gttgcagag 
      tttttgacg ctgagagac cacgaaatg gcattcccg 
      aagctggac gtctcgcta tttaatatc gagcagaac 
      gaggagtac cgtcaggga atgttggag aaagaaaaa 
      caagaacag gaatataaa gaaagggaa attgaggat 
      gcaggtgtt gagtatttg acgccgtat ttgactctg 
      ttgggaaat ccggagagc gtaaccccg gttgaggcg 
      ttcaagatt aaggagaag tgtctcagt gattttaaa 
      cagttgttg cttaatcgg gccatttgt attcaaaaa 
      cagttcgaa cactggacg cagtatttg aaagacagg 
      caacagtgg tacaacatt aatcaagac acggtcaca 
      gaagaggac gaaataaaa tattttcag gaagttaat 
      gacttgact tttctttta cagtgctac gaaattcgc 
      ttggctcgt catcgtgac ttatcattt ttcagatat 
      gaagccgtg gtcaggtat ttgaatgca caattaaaa 
      ccactgatc gaaacattt ggataa
    mRNA sequence
    • >TC033673 RA
      tggctccgg catggagca aaacgcacg acggagagg 
      gtgcttcgg cgacgtcgt ggtgtgttt gaggagggc 
      agcgctaat cctggagcc gtccctgag cgcggcgtg 
      gcgacggtc ggccgcggc ttcgagagc agatgccgc 
      gggccccgc gggcctccc gccgccccc cgatccctc 
      cgtcagtca cgcacacgg ggcgcgaac actcatccg 
      ttgcaaggg tttttacga cacgttgcg tggcccgga 
      atatgttag tccgagcct gtgcggatc agggtcgta 
      tcgaaggga ccggaaata ccgccgcga gaccctcct 
      ccgcgtctc cacgccgcc ggtttcgca aataacgat 
      cttctccta cgcgccgtg ggctttgtt ccggagcgg 
      tgttgtttc tggtcaaag cgaaattcg ggtcgcagt 
      agcaagcaa aacccgtcg tcccgtccc gtcccatcc 
      cgactctcc ctcatccca actcatcct ccttaaatc 
      cgtccctgt ccgaaccgc gacagctgc gggtagatc 
      acacggcag ggacaggac gcggagctt cgcggaccc 
      gtgcactaa ctacacacc gatcgccgc gagcgtcgg 
      cggccattg tgctttgcc ctgcttgcc cgccaaccg 
      acccttggc ccttgacac cagtggcgt atacccaag 
      ctcaacaca aaccaaatt taccaattt caaccgcca 
      aaagatcca aaccaagcc gaaaacaag agttataat 
      gtccggatt taaagaatc tcaggaaac ttctttgac 
      cccgttatg ttgtttttt gccaaaccg tttctactc 
      tcctcacac aaccttcgt ccgcaagtc cggacagcc 
      ccacgagct tgcttgtgt cctttgttc cctcctcat 
      aggcctcgg ttacgatgc ctacgtggt cttcggcta 
      cgcagtgcg agatataac gttacgaat aatgagtcg 
      tgtcgattc tccataccc ccccgatga gaaacaaga 
      agatgtacg tgacgaagg tgaagacaa taagaaata 
      caccgttaa gccccccag agatttgcg aagtaaatt 
      tttattgat gatggaaca gaaaaaaat cgatcagat 
      caaagccga acaagaacg cgaagccga actggaacg 
      gcagagaga agaggagga ggaacggcc gccgccgga 
      cgatctgga agggatgag gttccattt ttgggtcct 
      gataaggac agaaggtcg cgccatcga cgaaaatac 
      tttcgttga gccgagtac tggatatgc gcatccggt 
      taatagtcc gttgtacag tggcatcga aagtgtttg 
      gaatcattt gaattattg ggtcaatat gcaagactg 
      caaggaagg gttgggtac aatcgacta caatttgca 
      cgatgttga aaaatggga acatttgtt gatcggaga 
      gccgactca gtggagagt caccgtcga aaatttaaa 
      tatggatga cgaagaagc cgctttagc gaaaataat 
      ggaagaaaa acatttaga catgccgat atcttggag 
      catgaaaat atctatacc gcacaaagc tctaaaaca 
      aaaataccc cgaaggcat gatcagtac ttggtataa 
      aaaaacact agtcgaaga atacgcccc ttatattca 
      atatgacgg acttttaac caaaattat gagatataa 
      agactttga ttgtacgga atgtatcac agttgagca 
      gatttacga aaacagaca agacaaatt gaagaaaat 
      tgtttatga tttaaaaag cgatctagt cacggagta 
      ttttggccc tgggcgaga agatgccgt gattgagca 
      cacttactc gaataaaga caccgattc aaataagcg 
      gataattaa gttcaacca caaggcacg ctacgacgg 
      cttggaaaa aattgaatt cgagcggga acacatggt 
      tgaatatta catagaccg tgacgattt gttttattt 
      tcgcgaagt gttctacgc ccgcaaagg ccaaccccc 
      gcctgggtt caccgaaag tcaccgaaa attgattct 
      aagaataat cgagaaata cgaaaggaa ccataaagt 
      ccccgcgag tcacgatat cgcgacccg agaattcgc 
      aatcgtcga tagacagat tcttctaaa gtatcattt 
      cggggaagg aagagtcac ttgctcgac caggaattt 
      caataagcc ttcgattgc ggaatcggg ggagggaat 
      gaagttcac acctgaact cacctgggg ttatgtcgc 
      cgaaatcgg ggcgaagcc ccccacaca gctggagtt 
      gttcctttt attccagaa aatgttgaa agaagagga 
      gaaatccct gtcgcggat ccgcgaaat cgaggacca 
      ggttgcaga gtttttgac gctgagaga ccacgaaat 
      ggcattccc gaagctgga cgtctcgct atttaatat 
      cgagcagaa cgaggagta ccgtcaggg aatgttgga 
      gaaagaaaa acaagaaca ggaatataa agaaaggga 
      aattgagga tgcaggtgt tgagtattt gacgccgta 
      tttgactct gttgggaaa tccggagag cgtaacccc 
      ggttgaggc gttcaagat taaggagaa gtgtctcag 
      tgattttaa acagttgtt gcttaatcg ggccatttg 
      tattcaaaa acagttcga acactggac gcagtattt 
      gaaagacag gcaacagtg gtacaacat taatcaaga 
      cacggtcac agaagagga cgaaataaa atattttca 
      ggaagttaa tgacttgac ttttctttt acagtgcta 
      cgaaattcg cttggctcg tcatcgtga cttatcatt 
      tttcagata tgaagccgt ggtcaggta tttgaatgc 
      acaattaaa accactgat cgaaacatt tggataacg 
      tatattaaa aaataaaaa attatgaga tcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY