Status
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Gene information
General information
TC033673
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016384,TC016386 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6750458..6783030
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033673
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08719

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033673 RA
      MEQNARRRG CFGDVVVCL RRAALILEP SLSAAWRRS 
AAASRADAA GPAGLPPPP DPSVSHAHG ARTLIRCKG 
FYDTLRGPE YVSPSLCGS GSYRRDRKY RRETLLRVS 
TPPVSQITI FSYAPWALF RSGVVSGQS EIRVAVASK 
TRRPVPSHP DSPSSQLIL LKSVPVRTA TAAGRSHGR 
DRTRSFADP CTNYTPIAA SVGGHCALP CLPANRPLA 
LDTSGVYPS STQTKFTNF NRQKIQTKP KTRVIMSGF 
KESQETSLT PLCCFLPNR FYSPHTTFV RKSGQPHEL 
ACVLCSLLI GLGYDAYVV FGYAVRDIT LRIMSRVDS 
PYPPDEKQE DVRDEGEDN KKYTVKPPR DLRSKFLLM 
MEQKKIDQI KAEQEREAE LERQREEEE ERPPPDDLE 
GMRFHFWVL IRTEGRAID ENTFVEPST GYAHPVNSP 
LYSGIESVW NHLNYWVNM QDCKEGLGT IDYNLHDVE 
KWEHLLIGE PTQWRVTVE NLNMDDEEA ALAKIMEEK 
HLDMPISWS MKISIPHKA LKQKYPEGM ISTWYKKTL 
VEEYAPYIQ YDGLLTKIM RYKDFDCTE CITVEQIYE 
NRQDKLKKI VYDLKSDLV TEYFGPGRE DAVIEHTYS 
NKDTDSNKR IIKFNHKAR YDGLEKIEF EREHMVEYY 
IDRDDLFYF REVFYARKG QPPPGFTES HRKLILRII 
EKYERNHKV PASHDIATR EFAIVDRQI LLKYHFGEG 
RVTCSTRNF NKPSIAESG EGMKFTPEL TWGYVAEIG 
AKPPTQLEL FLLFQKMLK EEEKSLSRI REIEDQVAE 
FLTLRDHEM AFPKLDVSL FNIEQNEEY RQGMLEKEK 
QEQEYKERE IEDAGVEYL TPYLTLLGN PESVTPVEA 
FKIKEKCLS DFKQLLLNR AICIQKQFE HWTQYLKDR 
QQWYNINQD TVTEEDEIK YFQEVNDLT FLLQCYEIR 
LARHRDLSF FRYEAVVRY LNAQLKPLI ETFG
    CDS sequence
    • >TC033673 RA
      atggagcaa aacgcacga cggagaggg tgcttcggc 
gacgtcgtg gtgtgtttg aggagggca gcgctaatc 
ctggagccg tccctgagc gcggcgtgg cgacggtcg 
gccgcggct tcgagagca gatgccgcg ggccccgcg 
ggcctcccg ccgcccccc gatccctcc gtcagtcac 
gcacacggg gcgcgaaca ctcatccgt tgcaagggt 
ttttacgac acgttgcgt ggcccggaa tatgttagt 
ccgagcctg tgcggatca gggtcgtat cgaagggac 
cggaaatac cgccgcgag accctcctc cgcgtctcc 
acgccgccg gtttcgcaa ataacgatc ttctcctac 
gcgccgtgg gctttgttc cggagcggt gttgtttct 
ggtcaaagc gaaattcgg gtcgcagta gcaagcaaa 
acccgtcgt cccgtcccg tcccatccc gactctccc 
tcatcccaa ctcatcctc cttaaatcc gtccctgtc 
cgaaccgcg acagctgcg ggtagatca cacggcagg 
gacaggacg cggagcttc gcggacccg tgcactaac 
tacacaccg atcgccgcg agcgtcggc ggccattgt 
gctttgccc tgcttgccc gccaaccga cccttggcc 
cttgacacc agtggcgta tacccaagc tcaacacaa 
accaaattt accaatttc aaccgccaa aagatccaa 
accaagccg aaaacaaga gttataatg tccggattt 
aaagaatct caggaaact tctttgacc ccgttatgt 
tgttttttg ccaaaccgt ttctactct cctcacaca 
accttcgtc cgcaagtcc ggacagccc cacgagctt 
gcttgtgtc ctttgttcc ctcctcata ggcctcggt 
tacgatgcc tacgtggtc ttcggctac gcagtgcga 
gatataacg ttacgaata atgagtcgt gtcgattct 
ccatacccc cccgatgag aaacaagaa gatgtacgt 
gacgaaggt gaagacaat aagaaatac accgttaag 
ccccccaga gatttgcga agtaaattt ttattgatg 
atggaacag aaaaaaatc gatcagatc aaagccgaa 
caagaacgc gaagccgaa ctggaacgg cagagagaa 
gaggaggag gaacggccg ccgccggac gatctggaa 
gggatgagg ttccatttt tgggtcctg ataaggaca 
gaaggtcgc gccatcgac gaaaatact ttcgttgag 
ccgagtact ggatatgcg catccggtt aatagtccg 
ttgtacagt ggcatcgaa agtgtttgg aatcatttg 
aattattgg gtcaatatg caagactgc aaggaaggg 
ttgggtaca atcgactac aatttgcac gatgttgaa 
aaatgggaa catttgttg atcggagag ccgactcag 
tggagagtc accgtcgaa aatttaaat atggatgac 
gaagaagcc gctttagcg aaaataatg gaagaaaaa 
catttagac atgccgata tcttggagc atgaaaata 
tctataccg cacaaagct ctaaaacaa aaatacccc 
gaaggcatg atcagtact tggtataaa aaaacacta 
gtcgaagaa tacgcccct tatattcaa tatgacgga 
cttttaacc aaaattatg agatataaa gactttgat 
tgtacggaa tgtatcaca gttgagcag atttacgaa 
aacagacaa gacaaattg aagaaaatt gtttatgat 
ttaaaaagc gatctagtc acggagtat tttggccct 
gggcgagaa gatgccgtg attgagcac acttactcg 
aataaagac accgattca aataagcgg ataattaag 
ttcaaccac aaggcacgc tacgacggc ttggaaaaa 
attgaattc gagcgggaa cacatggtt gaatattac 
atagaccgt gacgatttg ttttatttt cgcgaagtg 
ttctacgcc cgcaaaggc caacccccg cctgggttc 
accgaaagt caccgaaaa ttgattcta agaataatc 
gagaaatac gaaaggaac cataaagtc cccgcgagt 
cacgatatc gcgacccga gaattcgca atcgtcgat 
agacagatt cttctaaag tatcatttc ggggaagga 
agagtcact tgctcgacc aggaatttc aataagcct 
tcgattgcg gaatcgggg gagggaatg aagttcaca 
cctgaactc acctggggt tatgtcgcc gaaatcggg 
gcgaagccc cccacacag ctggagttg ttcctttta 
ttccagaaa atgttgaaa gaagaggag aaatccctg 
tcgcggatc cgcgaaatc gaggaccag gttgcagag 
tttttgacg ctgagagac cacgaaatg gcattcccg 
aagctggac gtctcgcta tttaatatc gagcagaac 
gaggagtac cgtcaggga atgttggag aaagaaaaa 
caagaacag gaatataaa gaaagggaa attgaggat 
gcaggtgtt gagtatttg acgccgtat ttgactctg 
ttgggaaat ccggagagc gtaaccccg gttgaggcg 
ttcaagatt aaggagaag tgtctcagt gattttaaa 
cagttgttg cttaatcgg gccatttgt attcaaaaa 
cagttcgaa cactggacg cagtatttg aaagacagg 
caacagtgg tacaacatt aatcaagac acggtcaca 
gaagaggac gaaataaaa tattttcag gaagttaat 
gacttgact tttctttta cagtgctac gaaattcgc 
ttggctcgt catcgtgac ttatcattt ttcagatat 
gaagccgtg gtcaggtat ttgaatgca caattaaaa 
ccactgatc gaaacattt ggataa
    mRNA sequence
    • >TC033673 RA
      tggctccgg catggagca aaacgcacg acggagagg 
gtgcttcgg cgacgtcgt ggtgtgttt gaggagggc 
agcgctaat cctggagcc gtccctgag cgcggcgtg 
gcgacggtc ggccgcggc ttcgagagc agatgccgc 
gggccccgc gggcctccc gccgccccc cgatccctc 
cgtcagtca cgcacacgg ggcgcgaac actcatccg 
ttgcaaggg tttttacga cacgttgcg tggcccgga 
atatgttag tccgagcct gtgcggatc agggtcgta 
tcgaaggga ccggaaata ccgccgcga gaccctcct 
ccgcgtctc cacgccgcc ggtttcgca aataacgat 
cttctccta cgcgccgtg ggctttgtt ccggagcgg 
tgttgtttc tggtcaaag cgaaattcg ggtcgcagt 
agcaagcaa aacccgtcg tcccgtccc gtcccatcc 
cgactctcc ctcatccca actcatcct ccttaaatc 
cgtccctgt ccgaaccgc gacagctgc gggtagatc 
acacggcag ggacaggac gcggagctt cgcggaccc 
gtgcactaa ctacacacc gatcgccgc gagcgtcgg 
cggccattg tgctttgcc ctgcttgcc cgccaaccg 
acccttggc ccttgacac cagtggcgt atacccaag 
ctcaacaca aaccaaatt taccaattt caaccgcca 
aaagatcca aaccaagcc gaaaacaag agttataat 
gtccggatt taaagaatc tcaggaaac ttctttgac 
cccgttatg ttgtttttt gccaaaccg tttctactc 
tcctcacac aaccttcgt ccgcaagtc cggacagcc 
ccacgagct tgcttgtgt cctttgttc cctcctcat 
aggcctcgg ttacgatgc ctacgtggt cttcggcta 
cgcagtgcg agatataac gttacgaat aatgagtcg 
tgtcgattc tccataccc ccccgatga gaaacaaga 
agatgtacg tgacgaagg tgaagacaa taagaaata 
caccgttaa gccccccag agatttgcg aagtaaatt 
tttattgat gatggaaca gaaaaaaat cgatcagat 
caaagccga acaagaacg cgaagccga actggaacg 
gcagagaga agaggagga ggaacggcc gccgccgga 
cgatctgga agggatgag gttccattt ttgggtcct 
gataaggac agaaggtcg cgccatcga cgaaaatac 
tttcgttga gccgagtac tggatatgc gcatccggt 
taatagtcc gttgtacag tggcatcga aagtgtttg 
gaatcattt gaattattg ggtcaatat gcaagactg 
caaggaagg gttgggtac aatcgacta caatttgca 
cgatgttga aaaatggga acatttgtt gatcggaga 
gccgactca gtggagagt caccgtcga aaatttaaa 
tatggatga cgaagaagc cgctttagc gaaaataat 
ggaagaaaa acatttaga catgccgat atcttggag 
catgaaaat atctatacc gcacaaagc tctaaaaca 
aaaataccc cgaaggcat gatcagtac ttggtataa 
aaaaacact agtcgaaga atacgcccc ttatattca 
atatgacgg acttttaac caaaattat gagatataa 
agactttga ttgtacgga atgtatcac agttgagca 
gatttacga aaacagaca agacaaatt gaagaaaat 
tgtttatga tttaaaaag cgatctagt cacggagta 
ttttggccc tgggcgaga agatgccgt gattgagca 
cacttactc gaataaaga caccgattc aaataagcg 
gataattaa gttcaacca caaggcacg ctacgacgg 
cttggaaaa aattgaatt cgagcggga acacatggt 
tgaatatta catagaccg tgacgattt gttttattt 
tcgcgaagt gttctacgc ccgcaaagg ccaaccccc 
gcctgggtt caccgaaag tcaccgaaa attgattct 
aagaataat cgagaaata cgaaaggaa ccataaagt 
ccccgcgag tcacgatat cgcgacccg agaattcgc 
aatcgtcga tagacagat tcttctaaa gtatcattt 
cggggaagg aagagtcac ttgctcgac caggaattt 
caataagcc ttcgattgc ggaatcggg ggagggaat 
gaagttcac acctgaact cacctgggg ttatgtcgc 
cgaaatcgg ggcgaagcc ccccacaca gctggagtt 
gttcctttt attccagaa aatgttgaa agaagagga 
gaaatccct gtcgcggat ccgcgaaat cgaggacca 
ggttgcaga gtttttgac gctgagaga ccacgaaat 
ggcattccc gaagctgga cgtctcgct atttaatat 
cgagcagaa cgaggagta ccgtcaggg aatgttgga 
gaaagaaaa acaagaaca ggaatataa agaaaggga 
aattgagga tgcaggtgt tgagtattt gacgccgta 
tttgactct gttgggaaa tccggagag cgtaacccc 
ggttgaggc gttcaagat taaggagaa gtgtctcag 
tgattttaa acagttgtt gcttaatcg ggccatttg 
tattcaaaa acagttcga acactggac gcagtattt 
gaaagacag gcaacagtg gtacaacat taatcaaga 
cacggtcac agaagagga cgaaataaa atattttca 
ggaagttaa tgacttgac ttttctttt acagtgcta 
cgaaattcg cttggctcg tcatcgtga cttatcatt 
tttcagata tgaagccgt ggtcaggta tttgaatgc 
acaattaaa accactgat cgaaacatt tggataacg 
tatattaaa aaataaaaa attatgaga tcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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