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Gene information
General information
TC013296 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6420124..6421860
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013296
    iBeetle screen iB_08709

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013296 RA
      MQRFQSFLL FFITWEFNS AVQINYIKV PSAVKNDSG 
      SPVILDCNY SVRPDDSEL VVKWLLNDE VVYQWIPPQ 
      KPQSLGRLK NRVDLNYKA TDDPKSVYR AMKIWNPTT 
      DIAGEYRCF VSTFADEDF SVKNMIVFE PERSLVILK 
      QEFNYQSVN FTCSANEVY PAPKLILYK DRTDDFYNK 
      NPLQTLEWN TSRHPNGRF SVFVVAHAM VDSLVPGSL 
      IHCELRIPG TGYVKKRSL LYYPTAQTG CGRALDSCS 
      AQLVLILSL LISAFSI
    CDS sequence
    • >TC013296 RA
      atgcagcgg ttccaaagt ttcctactt ttctttatc 
      acttgggag ttcaattca gccgttcaa ataaattac 
      attaaagtt ccaagtgcg gtcaagaat gattcgggg 
      tcgccggta attcttgac tgtaattac tcggtcaga 
      cctgacgac agtgaatta gtagtcaaa tggttgttg 
      aacgacgaa gtggtttac cagtggata ccaccacaa 
      aaaccacag agtttagga cgtctgaaa aatcgggtt 
      gatttgaat tacaaagcg accgacgat ccgaaaagt 
      gtttacaga gccatgaaa atctggaat cctacgacg 
      gatattgcg ggggaatac cgatgtttc gtgtcgacg 
      ttcgctgat gaagatttt agtgtaaaa aacatgata 
      gtttttgaa cccgaacgc tctcttgtt atcttgaaa 
      caagaattt aactaccaa agtgttaat ttcacttgt 
      tccgctaat gaagtctat ccagcgccg aaactaatt 
      ctttacaag gaccggact gacgatttt tacaataaa 
      aatccgctc caaactctt gaatggaac actagtcgg 
      catccgaac ggccgattt tctgtgttt gttgtggct 
      cacgcaatg gtggattct ttagttcct ggatcttta 
      attcactgc gagttgcgc attcctggc actggatat 
      gttaagaag cgaagtttg ctttattat cccactgct 
      caaacagga tgtgggaga gctttagat agttgtagc 
      gctcaactc gtcttgatt ctgtcttta ctgatatct 
      gctttcagc atctaa
    mRNA sequence
    • >TC013296 RA
      ttcaaattt aaaaatgca gcggttcca aagtttcct 
      acttttctt tatcacttg ggagttcaa ttcagccgt 
      tcaaataaa ttacattaa agttccaag tgcggtcaa 
      gaatgattc ggggtcgcc ggtaattct tgactgtaa 
      ttactcggt cagacctga cgacagtga attagtagt 
      caaatggtt gttgaacga cgaagtggt ttaccagtg 
      gataccacc acaaaaacc acagagttt aggacgtct 
      gaaaaatcg ggttgattt gaattacaa agcgaccga 
      cgatccgaa aagtgttta cagagccat gaaaatctg 
      gaatcctac gacggatat tgcggggga ataccgatg 
      tttcgtgtc gacgttcgc tgatgaaga ttttagtgt 
      aaaaaacat gatagtttt tgaacccga acgctctct 
      tgttatctt gaaacaaga atttaacta ccaaagtgt 
      taatttcac ttgttccgc taatgaagt ctatccagc 
      gccgaaact aattcttta caaggaccg gactgacga 
      tttttacaa taaaaatcc gctccaaac tcttgaatg 
      gaacactag tcggcatcc gaacggccg attttctgt 
      gtttgttgt ggctcacgc aatggtgga ttctttagt 
      tcctggatc tttaattca ctgcgagtt gcgcattcc 
      tggcactgg atatgttaa gaagcgaag tttgcttta 
      ttatcccac tgctcaaac aggatgtgg gagagcttt 
      agatagttg tagcgctca actcgtctt gattctgtc 
      tttactgat atctgcttt cagcatcta attgtgaat 
      attctacta tcaaagtga tttacattt acaggactt 
      gcaaaaaaa atatgcaaa tttaataat aaatcatta 
      ttttgttgt cgtacaggg tgtaattat tgttcttgt 
      tataatgag aggatgtaa tcatatttg ttaatgtgt 
      tgatgtttg ttgtcgcca aatcgatcc tctcctcgc 
      tttcctccc ttaaattaa tattgcggt tcctgtaat 
      ttattgtcg cccaattag tgacaaaca tcgcgccct 
      tgtgacgtc gtattttac gagtcaccg tattatgtt 
      taaaaccca taaaacttt aagagattc aaaacttca 
      ggtgttcga cggtgcctt gcaccgtcc aatttgttt 
      cattgttat ttcaggtgt gaattatga aacttgtat 
      ttttgtaaa atagaaacc tcatgatta tttattaat 
      aaataaata aaaaaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY