Status
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Gene information
General information
TC011198 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19588201..19630128
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011198
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08707

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011198 RA
      MDNSSEYDY TTNISYNDT EEGGNQFIL PVWRQVLWS 
      ILYAGMVIV ATGGNLIVI WIVFSHKRM RTVTNYFLL 
      NLSVADTMV STLNVTFNF VYMLNSHWP FGELYCKIS 
      QFIAVLSVC ASVFSLMSI SIDRYMAIM TPLRPRMGR 
      TVTVLLAVT TWLLGVIIG SPSLMFFRT YTMPYKDGE 
      ERVICYPEW PDGTTNESM MEYAYNVGF LFVTYVVPI 
      GSMTYTYAR IGIELWGSQ SIGECTQRQ MENIRSKRR 
      VVKMMMVVV IIFAVCWLP YHLYFIVIS YFPEITNST 
      YIQETYLAI YWLAMSNSM YNPIIYCWM NARFRRGFK 
      QFFSCLPFI HVSPGALTR REVLTSRRR SYSGSPDHN 
      RIIRNGTIR MNYMTRPSP TSSTNTCYS NLPDELGYH 
      RNLEARRWK GEELRSVS
    CDS sequence
    • >TC011198 RA
      atggataac tcatcggaa tacgattac accaccaat 
      atcagctat aatgatacg gaggaaggt ggcaaccag 
      tttatactg ccggtgtgg agacaagtc ctgtggagc 
      atcctgtac gcaggcatg gtgattgtt gcgacaggt 
      ggtaattta atcgttatt tggatcgtt ttctcgcat 
      aaaaggatg cgaaccgtt acaaattat tttttgttg 
      aatctatcg gtggctgat accatggtc tcgactctc 
      aatgtcacg ttcaacttt gtctacatg ttaaactcg 
      cattggccc tttggtgaa ctgtactgc aaaatctcg 
      caatttata gctgttttg tccgtctgt gcatcggtg 
      ttttccctc atgtccatc tcaattgac agatacatg 
      gccataatg actccacta aggccgcga atgggccgc 
      actgtgacc gtgttatta gcagtgact acgtggctg 
      ctgggggtg attataggt agtccgtcc ctgatgttc 
      ttcagaaca tacacgatg ccgtataaa gatggggag 
      gaacgagtg atttgttat cctgagtgg ccggatggc 
      accaccaac gaatcgatg atggaatac gcctacaac 
      gtgggattc ctctttgtg acttacgtc gtgccgatc 
      ggatcgatg acgtacact tacgcccga atcgggatc 
      gaactctgg ggatcccag agcatcggc gaatgcacg 
      caacgccaa atggagaac attcggagc aaaaggcgg 
      gttgtgaaa atgatgatg gtcgtcgtg attattttc 
      gcagtgtgc tggcttcca tatcacctt tacttcatc 
      gtgatatcg tattttccg gaaataaca aactcgaca 
      tacatccag gaaacgtat ttggccatt tattggtta 
      gccatgagc aattccatg tacaatccg attatttat 
      tgctggatg aacgccaga ttccggcgg ggcttcaag 
      cagtttttc tcctgtctg ccgttcatt cacgtaagt 
      ccgggagct ttgacccgg cgcgaggtc ctcaccagc 
      cggagacgc tcctactcc ggctccccg gaccataac 
      cggatcatc cggaacgga accattcgc atgaactac 
      atgactcgc cccagcccc acgtcgtcg accaacacg 
      tgctactcg aacctcccc gacgagttg ggctaccac 
      cgtaacctg gaggcccgc cgatggaaa ggcgaagag 
      ctgcgttcc gtctcctga
    mRNA sequence
    • >TC011198 RA
      ataaattaa aaaattatt gcttccaat gtacttatt 
      tcaatttta tttcaggcg tttgcgtca tggaacttg 
      ataccgtct ccggttaaa gatgccaac ccctctgcc 
      caattctct cgagaaatg catctcgag gtccgtgac 
      aagatgaaa attctctcg tggaattcg cctttcgcg 
      taatactcc atccgccca cctgcacga tgaatttca 
      cacaagaat tcttgtaca cgaccggtt attccatca 
      tggataact catcggaat acgattaca ccaccaata 
      tcagctata atgatacgg aggaaggtg gcaaccagt 
      ttatactgc cggtgtgga gacaagtcc tgtggagca 
      tcctgtacg caggcatgg tgattgttg cgacaggtg 
      gtaatttaa tcgttattt ggatcgttt tctcgcata 
      aaaggatgc gaaccgtta caaattatt ttttgttga 
      atctatcgg tggctgata ccatggtct cgactctca 
      atgtcacgt tcaactttg tctacatgt taaactcgc 
      attggccct ttggtgaac tgtactgca aaatctcgc 
      aatttatag ctgttttgt ccgtctgtg catcggtgt 
      tttccctca tgtccatct caattgaca gatacatgg 
      ccataatga ctccactaa ggccgcgaa tgggccgca 
      ctgtgaccg tgttattag cagtgacta cgtggctgc 
      tgggggtga ttataggta gtccgtccc tgatgttct 
      tcagaacat acacgatgc cgtataaag atggggagg 
      aacgagtga tttgttatc ctgagtggc cggatggca 
      ccaccaacg aatcgatga tggaatacg cctacaacg 
      tgggattcc tctttgtga cttacgtcg tgccgatcg 
      gatcgatga cgtacactt acgcccgaa tcgggatcg 
      aactctggg gatcccaga gcatcggcg aatgcacgc 
      aacgccaaa tggagaaca ttcggagca aaaggcggg 
      ttgtgaaaa tgatgatgg tcgtcgtga ttattttcg 
      cagtgtgct ggcttccat atcaccttt acttcatcg 
      tgatatcgt attttccgg aaataacaa actcgacat 
      acatccagg aaacgtatt tggccattt attggttag 
      ccatgagca attccatgt acaatccga ttatttatt 
      gctggatga acgccagat tccggcggg gcttcaagc 
      agtttttct cctgtctgc cgttcattc acgtaagtc 
      cgggagctt tgacccggc gcgaggtcc tcaccagcc 
      ggagacgct cctactccg gctccccgg accataacc 
      ggatcatcc ggaacggaa ccattcgca tgaactaca 
      tgactcgcc ccagcccca cgtcgtcga ccaacacgt 
      gctactcga acctccccg acgagttgg gctaccacc 
      gtaacctgg aggcccgcc gatggaaag gcgaagagc 
      tgcgttccg tctcctgac aaaagcagt tggcccaaa 
      gccagaccg acgacaaga actccatca ggcaaagac 
      gcacctgga tctagtgcc aaacctcgc ggtccagcc 
      gacacttgc cctggactc gcgaggaaa ccaccgaag 
      aggtcgaca aaggcgtca ccgagacgg agatgtagg 
      aagtggagc ggctgtgac acatcttgg cgctaattt 
      agatagaac acagggtac taagagtta attcgtgac 
      ctcgactat gacatatcc catttatcc gaagtgtcg 
      tttctgtat tcagcacgg aggactttt tgccacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY