| General information | |||
| TC033657
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 6056774..6129709 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033657 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08706 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC033657 RA
MEEASGPRP PGVGTPDDG SVGEKAAER LAVRRKSFV PMSSMEEEN LRERLHQAQ LKQCSQIVC AEQKYTRDT FRRLFQGKE LLEQLFKLF DQDRDQCLP QENWIEFLK QRLTDEKQI DFAEQLESV AFCLCGDEP IHLEQFNQI FNAKGIVDK LFRLIDRDN SGIITSESI MEFLATITN TRPRTGFDK GSLDRLEQL FRQTVGNEK EIKREDFKK IVISKNPFF TERVFQIFD SDNSGSISL QEFLDAMHQ FAGKSPDDK IRFLFKVYD LDGDGLIQH KELQHVMRA CMEENGMQF SEEQIDDLT VAMFEDADT NNRGAITYE ALKNQLEKH GGLLENLSI SIDRWLVPP NTQNQPDSL VRKLMAMKP YQLTKPYVK NNYVYLIFL AGYLIVNAA LFISRAIEY RDKNWYTIF ARACGQCLN FNCMFVLVL MLRQCITFL RTRGYSSML PLDQHIYFH KLTGMFIFG YSVLHTIMH VCNFSLVVV DSPINVKNL TVNEWLFTN KPQIFGLVP GLANPTGFA LLFILLIMF VCSQAFVRR GGCFEVFYW THLLYVPFW ILVILHGPN FWKWFILPG CVYAIERIM RLVSMKSER GKTYISSGL LLPSKVTHL VIKRPLQFD FHPGDYVFV NIPAIAKYE WHPFTISSA PEQEDYMWL HIRGVGQWT NRLYEYFEK EQEKLHNGD IPPHNPPSA KKRLSNGSI DNNNQNPLK KIQATITRT LSNRDSNRK PGVQLVGFS NETFQDDSN KKPAESDFN RASCSNTDL TAYSPRPKK MTSDKKLHR LLFTEKAPL EKSLSMPDI QTKAKKRER LLVLKEYMR SESEKSFDE CQIRRARLQ SLGLAYLSP QNKSLAQSF RYMRNKPTI IAFKTPSLE NCEQRDSSS SLGSVGRQV SALSFSSSP EKQAEEGRA GFRTDFSPG TVPLKPMNY PVGKPLEPI FEKATPHDS DEELRERIL RDLPVGAGT EIYLDGPYG APSSHIFRA QHAVLIATG IGVTPFASI LQSIMHRYW KARHSCPRC KFSWASEIP PTVMNLRKV DFFWINRNQ RSFEWFVNL LSQLEIEQA ELGGAMERF LEMHMYITS ALQKTDMKA VGLQLALDL LHEKEKRDL ITGLKTRTN AGRPNWDKV FKQIQDQKK GKVTVFYCG PPQLARIIR VKCDQYGFS FRKEVF
- >TC033657 RA
atggaagag gcgagtggg ccgcgtcca cctggcgtc gggacgccc gatgacggc tcagttggt gaaaaggcg gccgagcgt cttgccgtc cgcaggaag tcgttcgtg cctatgtcc agcatggaa gaagagaac ctgagggag agactgcat caggcgcag ctgaagcag tgctcgcag atcgtctgc gccgagcag aagtacacg agggacacc ttcaggagg ctgtttcag ggcaaggag ctcttggaa caactcttc aaactcttc gaccaagac cgtgaccag tgtttacct caagaaaac tggatcgaa ttcctcaaa caaagactc acagatgaa aaacagata gatttcgcg gaacagctc gagagtgtt gcattctgc ctctgtggc gacgaaccc atccatctg gagcaattt aaccaaatt ttcaatgct aagggcatc gtagacaag ctatttagg ctaatagat cgagacaat tccgggata ataacttcc gaaagtatt atggaattt ctggcgact attacaaac acgaggcct cgaacgggg ttcgataaa ggcagccta gaccgatta gagcagtta ttcaggcaa actgtaggc aatgagaaa gaaatcaag agagaggac ttcaagaag atcgtcatt tcgaaaaat ccttttttt accgagcga gtgttccaa atcttcgac tcggacaat tccggttca atatctctg caggagttt ttggatgcg atgcaccaa ttcgccggc aaatctcct gatgataaa atacggttt ctgttcaaa gtttacgat ctggacggc gacggttta atacagcat aaagaactc caacatgtt atgagagcc tgtatggag gaaaacgga atgcaattc agcgaggag caaatcgac gacttgacg gtggcgatg ttcgaagat gccgacact aacaacagg ggggccatc acttacgaa gctctcaaa aaccagctg gaaaagcac ggggggctg ttagagaat ctatccatc agtattgat cgatggctt gtgcctccc aacacccag aaccagccg gattccctt gtgaggaag ctcatggca atgaaacct taccagtta accaagccc tacgtgaag aacaactac gtttatttg atatttcta gcaggatat ttgatagtt aacgcggct cttttcata tcacgtgcg atagaatac agagacaaa aactggtac acgatattt gcaagagct tgtggtcag tgcctcaac ttcaactgc atgtttgtc ttggtccta atgttgcgg caatgtatc acattcttg cgaacaaga ggatacagc tcgatgtta cccctcgac cagcacatc tattttcat aaactgacc gggatgttc atctttggt tacagtgtg ttgcacacg ataatgcac gtgtgtaat tttagttta gtcgtggtt gatagcccc ataaacgtc aaaaatttg accgtaaat gagtggctg ttcactaac aagccgcaa atatttggt ttagtacca ggtttagca aatccgact ggttttgcc ttgttattt attcttctg attatgttt gtttgttcg caagctttc gtgcgtaga ggaggctgt ttcgaggtg ttttactgg acacattta ctctatgtc ccattttgg attttggtc atccttcat gggcctaac ttctggaag tggttcatt cttccgggt tgtgtatat gctattgaa cgtataatg aggctagtt tcgatgaaa agtgagagg ggcaagact tacataagc tcggggcta cttctaccg tccaaagta acccatttg gtgattaaa agacccctc cagtttgat tttcatcca ggagattat gtttttgtc aatatacca gccattgcc aaatacgaa tggcatccc tttactatt agtagcgcc ccagagcaa gaagattac atgtggctc catatccgt ggtgtgggt caatggaca aatcgcctc tacgaatac tttgaaaaa gaacaagaa aagctccac aacggtgat attccaccg cacaatcct cccagtgca aaaaagaga ttgtctaat ggaagtatc gataacaac aaccaaaat ccgttgaag aagattcaa gcaactata actagaact ctatcaaat cgcgattca aaccgaaaa cccggagtc caacttgtg ggtttctcc aatgaaacg ttccaagac gacagtaac aaaaagcca gccgagagc gattttaat cgagcttct tgtagtaat actgatttg acggcttat tcgccaagg cccaagaaa atgacatct gataaaaag cttcatcgg ttgcttttc acagagaaa gcgccactg gaaaagtcg ctgtcgatg ccagatatt cagacaaaa gctaagaaa agggaacgg ctccttgtt ttgaaggaa tacatgcgg tccgagtcc gaaaaaagc ttcgacgag tgtcagata cgtcgtgct cgcctccag tctttaggc ctcgcatac ttgagcccc cagaataag tctttagcg cagagcttc cgttatatg cgtaacaaa ccaacaata atagcgttc aaaacaccc agtttggaa aactgtgag cagcgcgat tctagcagt tctttgggt tctgtcgga cgtcaagtt agcgctctt agtttttcc tcttctcct gaaaaacaa gccgaggaa ggccgagcc ggcttccgt accgatttc tctcccgga actgttcct ttaaaacca atgaattat cctgtagga aaaccttta gagcctatt tttgagaaa gcgacgcct catgatagt gatgaggaa cttagggaa cgcattttg cgcgacctg ccggtgggg gctgggacc gagatctat ctggatggc ccctacgga gccccttcg agccacata ttccgcgcc caacatgcc gttttgata gcgacagga attggagtc acccctttt gcttccatc ctccaatct atcatgcac agatattgg aaagcaaga cattcttgc ccacggtgt aaattttcg tgggccagc gagattcca cccacggtc atgaattta agaaaagtt gactttttc tggattaac cgaaatcaa cggtcgttc gaatggttt gttaattta ttgtcacag ttggaaatt gaacaagct gaacttggc ggcgcaatg gaacgtttc ctagaaatg cacatgtat atcaccagt gccttgcaa aaaaccgat atgaaagca gtgggtttg caattagct ttggatctg cttcatgaa aaagaaaaa agagacttg attactggc ttgaagact agaactaat gcgggacgc cctaactgg gacaaagtc ttcaagcag attcaagac cagaagaaa ggtaaagtt actgtcttc tactgtggt cctccacaa ctcgccagg attattaga gtaaaatgt gatcagtat ggatttagc tttcgtaag gaagtattt taa
- >TC033657 RA
aatggcttc ataaatcat acttaacta gccaagaat atctctccc gggattctt taatgagtg gaaaatatg gtacatacc ttgaactat aatccaaaa tctgagccc aaattattg gatgcacaa cactgacgt caacgaaac gaggtgaag acctgatca catgccaca tgtcaactt tcgttttga caagggcga agcaccgcc actccacaa gaccttgag tatgttgag gaaaaaaac acttggaag tggtgcaaa ttgctctcg cttcccacc ccattttgg gctaagtga gtggcagcg gcctcctgc gatgtctac ctcttgcca gttcagcgc tgattaaga gtagaagaa ccatctcgt ggcatagac ccggttcta gcatcttgg agtagtttt ttgtggccg tccgcacgc gtcttcttc gtgtgaccg ttggtgtga ttgttgtgt tctttttaa tgcgtgtga gtgaaaatg gaagaggcg agtgggccg cgtccacct ggcgtcggg acgcccgat gacggctca gttggtgaa aaggcggcc gagcgtctt gccgtccgc aggaagtcg ttcgtgcct atgtccagc atggaagaa gagaacctg agggagaga ctgcatcag gcgcagctg aagcagtgc tcgcagatc gtctgcgcc gagcagaag tacacgagg gacaccttc aggaggctg tttcagggc aaggagctc ttggaacaa ctcttcaaa ctcttcgac caagaccgt gaccagtgt ttacctcaa gaaaactgg atcgaattc ctcaaacaa agactcaca gatgaaaaa cagatagat ttcgcggaa cagctcgag agtgttgca ttctgcctc tgtggcgac gaacccatc catctggag caatttaac caaattttc aatgctaag ggcatcgta gacaagcta tttaggcta atagatcga gacaattcc gggataata acttccgaa agtattatg gaatttctg gcgactatt acaaacacg aggcctcga acggggttc gataaaggc agcctagac cgattagag cagttattc aggcaaact gtaggcaat gagaaagaa atcaagaga gaggacttc aagaagatc gtcatttcg aaaaatcct ttttttacc gagcgagtg ttccaaatc ttcgactcg gacaattcc ggttcaata tctctgcag gagtttttg gatgcgatg caccaattc gccggcaaa tctcctgat gataaaata cggtttctg ttcaaagtt tacgatctg gacggcgac ggtttaata cagcataaa gaactccaa catgttatg agagcctgt atggaggaa aacggaatg caattcagc gaggagcaa atcgacgac ttgacggtg gcgatgttc gaagatgcc gacactaac aacaggggg gccatcact tacgaagct ctcaaaaac cagctggaa aagcacggg gggctgtta gagaatcta tccatcagt attgatcga tggcttgtg cctcccaac acccagaac cagccggat tcccttgtg aggaagctc atggcaatg aaaccttac cagttaacc aagccctac gtgaagaac aactacgtt tatttgata tttctagca ggatatttg atagttaac gcggctctt ttcatatca cgtgcgata gaatacaga gacaaaaac tggtacacg atatttgca agagcttgt ggtcagtgc ctcaacttc aactgcatg tttgtcttg gtcctaatg ttgcggcaa tgtatcaca ttcttgcga acaagagga tacagctcg atgttaccc ctcgaccag cacatctat tttcataaa ctgaccggg atgttcatc tttggttac agtgtgttg cacacgata atgcacgtg tgtaatttt agtttagtc gtggttgat agccccata aacgtcaaa aatttgacc gtaaatgag tggctgttc actaacaag ccgcaaata tttggttta gtaccaggt ttagcaaat ccgactggt tttgccttg ttatttatt cttctgatt atgtttgtt tgttcgcaa gctttcgtg cgtagagga ggctgtttc gaggtgttt tactggaca catttactc tatgtccca ttttggatt ttggtcatc cttcatggg cctaacttc tggaagtgg ttcattctt ccgggttgt gtatatgct attgaacgt ataatgagg ctagtttcg atgaaaagt gagaggggc aagacttac ataagctcg gggctactt ctaccgtcc aaagtaacc catttggtg attaaaaga cccctccag tttgatttt catccagga gattatgtt tttgtcaat ataccagcc attgccaaa tacgaatgg catcccttt actattagt agcgcccca gagcaagaa gattacatg tggctccat atccgtggt gtgggtcaa tggacaaat cgcctctac gaatacttt gaaaaagaa caagaaaag ctccacaac ggtgatatt ccaccgcac aatcctccc agtgcaaaa aagagattg tctaatgga agtatcgat aacaacaac caaaatccg ttgaagaag attcaagca actataact agaactcta tcaaatcgc gattcaaac cgaaaaccc ggagtccaa cttgtgggt ttctccaat gaaacgttc caagacgac agtaacaaa aagccagcc gagagcgat tttaatcga gcttcttgt agtaatact gatttgacg gcttattcg ccaaggccc aagaaaatg acatctgat aaaaagctt catcggttg cttttcaca gagaaagcg ccactggaa aagtcgctg tcgatgcca gatattcag acaaaagct aagaaaagg gaacggctc cttgttttg aaggaatac atgcggtcc gagtccgaa aaaagcttc gacgagtgt cagatacgt cgtgctcgc ctccagtct ttaggcctc gcatacttg agcccccag aataagtct ttagcgcag agcttccgt tatatgcgt aacaaacca acaataata gcgttcaaa acacccagt ttggaaaac tgtgagcag cgcgattct agcagttct ttgggttct gtcggacgt caagttagc gctcttagt ttttcctct tctcctgaa aaacaagcc gaggaaggc cgagccggc ttccgtacc gatttctct cccggaact gttccttta aaaccaatg aattatcct gtaggaaaa cctttagag cctattttt gagaaagcg acgcctcat gatagtgat gaggaactt agggaacgc attttgcgc gacctgccg gtgggggct gggaccgag atctatctg gatggcccc tacggagcc ccttcgagc cacatattc cgcgcccaa catgccgtt ttgatagcg acaggaatt ggagtcacc ccttttgct tccatcctc caatctatc atgcacaga tattggaaa gcaagacat tcttgccca cggtgtaaa ttttcgtgg gccagcgag attccaccc acggtcatg aatttaaga aaagttgac tttttctgg attaaccga aatcaacgg tcgttcgaa tggtttgtt aatttattg tcacagttg gaaattgaa caagctgaa cttggcggc gcaatggaa cgtttccta gaaatgcac atgtatatc accagtgcc ttgcaaaaa accgatatg aaagcagtg ggtttgcaa ttagctttg gatctgctt catgaaaaa gaaaaaaga gacttgatt actggcttg aagactaga actaatgcg ggacgccct aactgggac aaagtcttc aagcagatt caagaccag aagaaaggt aaagttact gtcttctac tgtggtcct ccacaactc gccaggatt attagagta aaatgtgat cagtatgga tttagcttt cgtaaggaa gtattttaa tgtcatttg tatattgta atcttgaat tgtaccata gttatttat attatttat ttctgttgt atattaagt ccttactta ctcgtactt ttctattaa ttttaacac tcaagtctt tatgcctac agtcagttt ttaaaggta atgggtctt tttataggt atttatctg tttagtgtt agtgcactg tttgtatag taaatgtga ggtgaaata tatttattt tcatttt
