Status
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Gene information
General information
TC033657
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013282,TC013283
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6056774..6129709
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033657
    iBeetle screen iB_08706

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033657 RA
      MEEASGPRP PGVGTPDDG SVGEKAAER LAVRRKSFV 
      PMSSMEEEN LRERLHQAQ LKQCSQIVC AEQKYTRDT 
      FRRLFQGKE LLEQLFKLF DQDRDQCLP QENWIEFLK 
      QRLTDEKQI DFAEQLESV AFCLCGDEP IHLEQFNQI 
      FNAKGIVDK LFRLIDRDN SGIITSESI MEFLATITN 
      TRPRTGFDK GSLDRLEQL FRQTVGNEK EIKREDFKK 
      IVISKNPFF TERVFQIFD SDNSGSISL QEFLDAMHQ 
      FAGKSPDDK IRFLFKVYD LDGDGLIQH KELQHVMRA 
      CMEENGMQF SEEQIDDLT VAMFEDADT NNRGAITYE 
      ALKNQLEKH GGLLENLSI SIDRWLVPP NTQNQPDSL 
      VRKLMAMKP YQLTKPYVK NNYVYLIFL AGYLIVNAA 
      LFISRAIEY RDKNWYTIF ARACGQCLN FNCMFVLVL 
      MLRQCITFL RTRGYSSML PLDQHIYFH KLTGMFIFG 
      YSVLHTIMH VCNFSLVVV DSPINVKNL TVNEWLFTN 
      KPQIFGLVP GLANPTGFA LLFILLIMF VCSQAFVRR 
      GGCFEVFYW THLLYVPFW ILVILHGPN FWKWFILPG 
      CVYAIERIM RLVSMKSER GKTYISSGL LLPSKVTHL 
      VIKRPLQFD FHPGDYVFV NIPAIAKYE WHPFTISSA 
      PEQEDYMWL HIRGVGQWT NRLYEYFEK EQEKLHNGD 
      IPPHNPPSA KKRLSNGSI DNNNQNPLK KIQATITRT 
      LSNRDSNRK PGVQLVGFS NETFQDDSN KKPAESDFN 
      RASCSNTDL TAYSPRPKK MTSDKKLHR LLFTEKAPL 
      EKSLSMPDI QTKAKKRER LLVLKEYMR SESEKSFDE 
      CQIRRARLQ SLGLAYLSP QNKSLAQSF RYMRNKPTI 
      IAFKTPSLE NCEQRDSSS SLGSVGRQV SALSFSSSP 
      EKQAEEGRA GFRTDFSPG TVPLKPMNY PVGKPLEPI 
      FEKATPHDS DEELRERIL RDLPVGAGT EIYLDGPYG 
      APSSHIFRA QHAVLIATG IGVTPFASI LQSIMHRYW 
      KARHSCPRC KFSWASEIP PTVMNLRKV DFFWINRNQ 
      RSFEWFVNL LSQLEIEQA ELGGAMERF LEMHMYITS 
      ALQKTDMKA VGLQLALDL LHEKEKRDL ITGLKTRTN 
      AGRPNWDKV FKQIQDQKK GKVTVFYCG PPQLARIIR 
      VKCDQYGFS FRKEVF
    CDS sequence
    • >TC033657 RA
      atggaagag gcgagtggg ccgcgtcca cctggcgtc 
      gggacgccc gatgacggc tcagttggt gaaaaggcg 
      gccgagcgt cttgccgtc cgcaggaag tcgttcgtg 
      cctatgtcc agcatggaa gaagagaac ctgagggag 
      agactgcat caggcgcag ctgaagcag tgctcgcag 
      atcgtctgc gccgagcag aagtacacg agggacacc 
      ttcaggagg ctgtttcag ggcaaggag ctcttggaa 
      caactcttc aaactcttc gaccaagac cgtgaccag 
      tgtttacct caagaaaac tggatcgaa ttcctcaaa 
      caaagactc acagatgaa aaacagata gatttcgcg 
      gaacagctc gagagtgtt gcattctgc ctctgtggc 
      gacgaaccc atccatctg gagcaattt aaccaaatt 
      ttcaatgct aagggcatc gtagacaag ctatttagg 
      ctaatagat cgagacaat tccgggata ataacttcc 
      gaaagtatt atggaattt ctggcgact attacaaac 
      acgaggcct cgaacgggg ttcgataaa ggcagccta 
      gaccgatta gagcagtta ttcaggcaa actgtaggc 
      aatgagaaa gaaatcaag agagaggac ttcaagaag 
      atcgtcatt tcgaaaaat ccttttttt accgagcga 
      gtgttccaa atcttcgac tcggacaat tccggttca 
      atatctctg caggagttt ttggatgcg atgcaccaa 
      ttcgccggc aaatctcct gatgataaa atacggttt 
      ctgttcaaa gtttacgat ctggacggc gacggttta 
      atacagcat aaagaactc caacatgtt atgagagcc 
      tgtatggag gaaaacgga atgcaattc agcgaggag 
      caaatcgac gacttgacg gtggcgatg ttcgaagat 
      gccgacact aacaacagg ggggccatc acttacgaa 
      gctctcaaa aaccagctg gaaaagcac ggggggctg 
      ttagagaat ctatccatc agtattgat cgatggctt 
      gtgcctccc aacacccag aaccagccg gattccctt 
      gtgaggaag ctcatggca atgaaacct taccagtta 
      accaagccc tacgtgaag aacaactac gtttatttg 
      atatttcta gcaggatat ttgatagtt aacgcggct 
      cttttcata tcacgtgcg atagaatac agagacaaa 
      aactggtac acgatattt gcaagagct tgtggtcag 
      tgcctcaac ttcaactgc atgtttgtc ttggtccta 
      atgttgcgg caatgtatc acattcttg cgaacaaga 
      ggatacagc tcgatgtta cccctcgac cagcacatc 
      tattttcat aaactgacc gggatgttc atctttggt 
      tacagtgtg ttgcacacg ataatgcac gtgtgtaat 
      tttagttta gtcgtggtt gatagcccc ataaacgtc 
      aaaaatttg accgtaaat gagtggctg ttcactaac 
      aagccgcaa atatttggt ttagtacca ggtttagca 
      aatccgact ggttttgcc ttgttattt attcttctg 
      attatgttt gtttgttcg caagctttc gtgcgtaga 
      ggaggctgt ttcgaggtg ttttactgg acacattta 
      ctctatgtc ccattttgg attttggtc atccttcat 
      gggcctaac ttctggaag tggttcatt cttccgggt 
      tgtgtatat gctattgaa cgtataatg aggctagtt 
      tcgatgaaa agtgagagg ggcaagact tacataagc 
      tcggggcta cttctaccg tccaaagta acccatttg 
      gtgattaaa agacccctc cagtttgat tttcatcca 
      ggagattat gtttttgtc aatatacca gccattgcc 
      aaatacgaa tggcatccc tttactatt agtagcgcc 
      ccagagcaa gaagattac atgtggctc catatccgt 
      ggtgtgggt caatggaca aatcgcctc tacgaatac 
      tttgaaaaa gaacaagaa aagctccac aacggtgat 
      attccaccg cacaatcct cccagtgca aaaaagaga 
      ttgtctaat ggaagtatc gataacaac aaccaaaat 
      ccgttgaag aagattcaa gcaactata actagaact 
      ctatcaaat cgcgattca aaccgaaaa cccggagtc 
      caacttgtg ggtttctcc aatgaaacg ttccaagac 
      gacagtaac aaaaagcca gccgagagc gattttaat 
      cgagcttct tgtagtaat actgatttg acggcttat 
      tcgccaagg cccaagaaa atgacatct gataaaaag 
      cttcatcgg ttgcttttc acagagaaa gcgccactg 
      gaaaagtcg ctgtcgatg ccagatatt cagacaaaa 
      gctaagaaa agggaacgg ctccttgtt ttgaaggaa 
      tacatgcgg tccgagtcc gaaaaaagc ttcgacgag 
      tgtcagata cgtcgtgct cgcctccag tctttaggc 
      ctcgcatac ttgagcccc cagaataag tctttagcg 
      cagagcttc cgttatatg cgtaacaaa ccaacaata 
      atagcgttc aaaacaccc agtttggaa aactgtgag 
      cagcgcgat tctagcagt tctttgggt tctgtcgga 
      cgtcaagtt agcgctctt agtttttcc tcttctcct 
      gaaaaacaa gccgaggaa ggccgagcc ggcttccgt 
      accgatttc tctcccgga actgttcct ttaaaacca 
      atgaattat cctgtagga aaaccttta gagcctatt 
      tttgagaaa gcgacgcct catgatagt gatgaggaa 
      cttagggaa cgcattttg cgcgacctg ccggtgggg 
      gctgggacc gagatctat ctggatggc ccctacgga 
      gccccttcg agccacata ttccgcgcc caacatgcc 
      gttttgata gcgacagga attggagtc acccctttt 
      gcttccatc ctccaatct atcatgcac agatattgg 
      aaagcaaga cattcttgc ccacggtgt aaattttcg 
      tgggccagc gagattcca cccacggtc atgaattta 
      agaaaagtt gactttttc tggattaac cgaaatcaa 
      cggtcgttc gaatggttt gttaattta ttgtcacag 
      ttggaaatt gaacaagct gaacttggc ggcgcaatg 
      gaacgtttc ctagaaatg cacatgtat atcaccagt 
      gccttgcaa aaaaccgat atgaaagca gtgggtttg 
      caattagct ttggatctg cttcatgaa aaagaaaaa 
      agagacttg attactggc ttgaagact agaactaat 
      gcgggacgc cctaactgg gacaaagtc ttcaagcag 
      attcaagac cagaagaaa ggtaaagtt actgtcttc 
      tactgtggt cctccacaa ctcgccagg attattaga 
      gtaaaatgt gatcagtat ggatttagc tttcgtaag 
      gaagtattt taa
    mRNA sequence
    • >TC033657 RA
      aatggcttc ataaatcat acttaacta gccaagaat 
      atctctccc gggattctt taatgagtg gaaaatatg 
      gtacatacc ttgaactat aatccaaaa tctgagccc 
      aaattattg gatgcacaa cactgacgt caacgaaac 
      gaggtgaag acctgatca catgccaca tgtcaactt 
      tcgttttga caagggcga agcaccgcc actccacaa 
      gaccttgag tatgttgag gaaaaaaac acttggaag 
      tggtgcaaa ttgctctcg cttcccacc ccattttgg 
      gctaagtga gtggcagcg gcctcctgc gatgtctac 
      ctcttgcca gttcagcgc tgattaaga gtagaagaa 
      ccatctcgt ggcatagac ccggttcta gcatcttgg 
      agtagtttt ttgtggccg tccgcacgc gtcttcttc 
      gtgtgaccg ttggtgtga ttgttgtgt tctttttaa 
      tgcgtgtga gtgaaaatg gaagaggcg agtgggccg 
      cgtccacct ggcgtcggg acgcccgat gacggctca 
      gttggtgaa aaggcggcc gagcgtctt gccgtccgc 
      aggaagtcg ttcgtgcct atgtccagc atggaagaa 
      gagaacctg agggagaga ctgcatcag gcgcagctg 
      aagcagtgc tcgcagatc gtctgcgcc gagcagaag 
      tacacgagg gacaccttc aggaggctg tttcagggc 
      aaggagctc ttggaacaa ctcttcaaa ctcttcgac 
      caagaccgt gaccagtgt ttacctcaa gaaaactgg 
      atcgaattc ctcaaacaa agactcaca gatgaaaaa 
      cagatagat ttcgcggaa cagctcgag agtgttgca 
      ttctgcctc tgtggcgac gaacccatc catctggag 
      caatttaac caaattttc aatgctaag ggcatcgta 
      gacaagcta tttaggcta atagatcga gacaattcc 
      gggataata acttccgaa agtattatg gaatttctg 
      gcgactatt acaaacacg aggcctcga acggggttc 
      gataaaggc agcctagac cgattagag cagttattc 
      aggcaaact gtaggcaat gagaaagaa atcaagaga 
      gaggacttc aagaagatc gtcatttcg aaaaatcct 
      ttttttacc gagcgagtg ttccaaatc ttcgactcg 
      gacaattcc ggttcaata tctctgcag gagtttttg 
      gatgcgatg caccaattc gccggcaaa tctcctgat 
      gataaaata cggtttctg ttcaaagtt tacgatctg 
      gacggcgac ggtttaata cagcataaa gaactccaa 
      catgttatg agagcctgt atggaggaa aacggaatg 
      caattcagc gaggagcaa atcgacgac ttgacggtg 
      gcgatgttc gaagatgcc gacactaac aacaggggg 
      gccatcact tacgaagct ctcaaaaac cagctggaa 
      aagcacggg gggctgtta gagaatcta tccatcagt 
      attgatcga tggcttgtg cctcccaac acccagaac 
      cagccggat tcccttgtg aggaagctc atggcaatg 
      aaaccttac cagttaacc aagccctac gtgaagaac 
      aactacgtt tatttgata tttctagca ggatatttg 
      atagttaac gcggctctt ttcatatca cgtgcgata 
      gaatacaga gacaaaaac tggtacacg atatttgca 
      agagcttgt ggtcagtgc ctcaacttc aactgcatg 
      tttgtcttg gtcctaatg ttgcggcaa tgtatcaca 
      ttcttgcga acaagagga tacagctcg atgttaccc 
      ctcgaccag cacatctat tttcataaa ctgaccggg 
      atgttcatc tttggttac agtgtgttg cacacgata 
      atgcacgtg tgtaatttt agtttagtc gtggttgat 
      agccccata aacgtcaaa aatttgacc gtaaatgag 
      tggctgttc actaacaag ccgcaaata tttggttta 
      gtaccaggt ttagcaaat ccgactggt tttgccttg 
      ttatttatt cttctgatt atgtttgtt tgttcgcaa 
      gctttcgtg cgtagagga ggctgtttc gaggtgttt 
      tactggaca catttactc tatgtccca ttttggatt 
      ttggtcatc cttcatggg cctaacttc tggaagtgg 
      ttcattctt ccgggttgt gtatatgct attgaacgt 
      ataatgagg ctagtttcg atgaaaagt gagaggggc 
      aagacttac ataagctcg gggctactt ctaccgtcc 
      aaagtaacc catttggtg attaaaaga cccctccag 
      tttgatttt catccagga gattatgtt tttgtcaat 
      ataccagcc attgccaaa tacgaatgg catcccttt 
      actattagt agcgcccca gagcaagaa gattacatg 
      tggctccat atccgtggt gtgggtcaa tggacaaat 
      cgcctctac gaatacttt gaaaaagaa caagaaaag 
      ctccacaac ggtgatatt ccaccgcac aatcctccc 
      agtgcaaaa aagagattg tctaatgga agtatcgat 
      aacaacaac caaaatccg ttgaagaag attcaagca 
      actataact agaactcta tcaaatcgc gattcaaac 
      cgaaaaccc ggagtccaa cttgtgggt ttctccaat 
      gaaacgttc caagacgac agtaacaaa aagccagcc 
      gagagcgat tttaatcga gcttcttgt agtaatact 
      gatttgacg gcttattcg ccaaggccc aagaaaatg 
      acatctgat aaaaagctt catcggttg cttttcaca 
      gagaaagcg ccactggaa aagtcgctg tcgatgcca 
      gatattcag acaaaagct aagaaaagg gaacggctc 
      cttgttttg aaggaatac atgcggtcc gagtccgaa 
      aaaagcttc gacgagtgt cagatacgt cgtgctcgc 
      ctccagtct ttaggcctc gcatacttg agcccccag 
      aataagtct ttagcgcag agcttccgt tatatgcgt 
      aacaaacca acaataata gcgttcaaa acacccagt 
      ttggaaaac tgtgagcag cgcgattct agcagttct 
      ttgggttct gtcggacgt caagttagc gctcttagt 
      ttttcctct tctcctgaa aaacaagcc gaggaaggc 
      cgagccggc ttccgtacc gatttctct cccggaact 
      gttccttta aaaccaatg aattatcct gtaggaaaa 
      cctttagag cctattttt gagaaagcg acgcctcat 
      gatagtgat gaggaactt agggaacgc attttgcgc 
      gacctgccg gtgggggct gggaccgag atctatctg 
      gatggcccc tacggagcc ccttcgagc cacatattc 
      cgcgcccaa catgccgtt ttgatagcg acaggaatt 
      ggagtcacc ccttttgct tccatcctc caatctatc 
      atgcacaga tattggaaa gcaagacat tcttgccca 
      cggtgtaaa ttttcgtgg gccagcgag attccaccc 
      acggtcatg aatttaaga aaagttgac tttttctgg 
      attaaccga aatcaacgg tcgttcgaa tggtttgtt 
      aatttattg tcacagttg gaaattgaa caagctgaa 
      cttggcggc gcaatggaa cgtttccta gaaatgcac 
      atgtatatc accagtgcc ttgcaaaaa accgatatg 
      aaagcagtg ggtttgcaa ttagctttg gatctgctt 
      catgaaaaa gaaaaaaga gacttgatt actggcttg 
      aagactaga actaatgcg ggacgccct aactgggac 
      aaagtcttc aagcagatt caagaccag aagaaaggt 
      aaagttact gtcttctac tgtggtcct ccacaactc 
      gccaggatt attagagta aaatgtgat cagtatgga 
      tttagcttt cgtaaggaa gtattttaa tgtcatttg 
      tatattgta atcttgaat tgtaccata gttatttat 
      attatttat ttctgttgt atattaagt ccttactta 
      ctcgtactt ttctattaa ttttaacac tcaagtctt 
      tatgcctac agtcagttt ttaaaggta atgggtctt 
      tttataggt atttatctg tttagtgtt agtgcactg 
      tttgtatag taaatgtga ggtgaaata tatttattt 
      tcatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY