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Gene information
General information
TC030549 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4859599..4861406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030549
    iBeetle screen iB_08704

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva segment size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    antenna pedicellus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs pretarsus not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax not fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax not fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen not fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen not fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030549 RA
      MLPKIKLSR DISRQELLI YVLVWLCAV LYAIACFTN 
ESNKHFQYY DDAYSDFTQ GWSFLHRKK DVADFEWQS 
MKFLITNLY SWVLLLTVV SEVLRKCSS VRFVQLWQI 
LVTVLYIIS NLGPFALLV ILLQPIIFS FVLTKHRKL 
LIWLCNGLV LVLISTYKN FLAREEFLI WLKLNEQTA 
YITMITIFW MNLRCVSYG LDLIDSKGR QDLIAFLSY 
CLYLPNLQT GPFIPYKDF INSHEHNNN LFSRLSQLF 
RDVVRFLFW LFFAELCLH FIYVNAAGF HPEWVESLD 
SWSLYGYGY TMGQFFHIK YLVTYGLAT SFAKFDNIK 
VPSLPVCIG RIHLYSDMW KYFDVGLYN FLLRYIYIP 
SMNNGFVRS RSLSSFLCF LFVYVWHGT DNYILIWTL 
LNYIGVIIE NCSHWFSAI YLQNSKLCK GLGPKWGRR 
INCMAASPL LAVSAISNF YFFARMEIG NIFLNRFLN 
DNYWSNFVL LAILYNCCQ VSTEVRRKQ SNKNSRLKD 
L
    CDS sequence
    • >TC030549 RA
      atgctccca aaaataaaa ttgtctcga gacatttca 
cgccaagaa ttgttaatt tatgtcctg gtatggtta 
tgtgctgtc ctttacgca attgcgtgt tttacgaat 
gagagcaac aaacatttt cagtactac gatgacgca 
tattcagat tttacacaa ggctggtct ttcttacac 
agaaaaaaa gatgtggct gattttgaa tggcagtct 
atgaagttt ttgataacg aatttatat tcatgggtc 
cttttatta acagttgta tcagaagtt ttacgaaaa 
tgtagttca gtcaggttt gtgcaattg tggcaaatt 
ctggtcaca gttttgtac ataatcagt aatttgggc 
ccgtttgca ctgttagtc atactttta cagccaata 
atattttca tttgtgtta accaagcac aggaaattg 
ttgatttgg ttgtgcaac ggccttgtc ctagtgcta 
atttccacg tataagaat tttcttgca agagaggaa 
tttttgatt tggttgaaa ctaaacgaa caaactgct 
tacataact atgataacg atattttgg atgaacttg 
cgttgtgtt agctacggt ttagacctg atagattcc 
aaagggagg caagatttg atcgcattt ttgtcatac 
tgtctgtac cttccaaat ttacaaact ggaccattt 
attccctac aaagatttt atcaattct catgaacac 
aacaataat ttatttagc cgcctttca caactattc 
cgggacgtg gttcggttt ttattttgg ttgtttttt 
gccgaattg tgtttacat tttatttat gtgaacgct 
gcgggattt caccccgag tgggtcgaa tcactggat 
tcgtggtcg ttgtatggc tatggatac accatgggc 
cagtttttc cacattaaa tatttggtc acgtatgga 
ttagcaaca agttttgca aaattcgat aacattaaa 
gtgccttct ttaccggta tgtatcgga agaatacac 
ttgtactcc gacatgtgg aagtatttc gatgtgggc 
ctttacaat tttttatta cgatatatt tacatcccc 
tcgatgaac aacggattt gttcgtagc cggtccttg 
tctagtttc ctttgcttt ctttttgtc tacgtctgg 
cacggaacc gataattac atattaatt tggacgctt 
ttgaattac attggtgtg ataattgag aattgtagt 
cactggttt agtgccatt tatttgcaa aactcgaag 
ttgtgtaaa ggtttgggg cccaaatgg ggccggcga 
atcaactgc atggctgca agccccctt ttggccgtg 
tcggcaatt tcaaatttt tattttttc gcccggatg 
gaaatcgga aatattttt cttaacaga tttttgaat 
gataattat tggtccaac ttcgtacta ttggccatt 
ttatacaac tgttgtcag gtttctacc gaagtaagg 
aggaaacaa tctaataaa aactcaaga ctgaaagac 
ttgtag
    mRNA sequence
    • >TC030549 RA
      acctccaaa catggcaag tccacaatg ctcccaaaa 
ataaaattg tctcgagac atttcacgc caagaattg 
ttaatttat gtcctggta tggttatgt gctgtcctt 
tacgcaatt gcgtgtttt acgaatgag agcaacaaa 
cattttcag tactacgat gacgcatat tcagatttt 
acacaaggc tggtctttc ttacacaga aaaaaagat 
gtggctgat tttgaatgg cagtctatg aagtttttg 
ataacgaat ttatattca tgggtcctt ttattaaca 
gttgtatca gaagtttta cgaaaatgt agttcagtc 
aggtttgtg caattgtgg caaattctg gtcacagtt 
ttgtacata atcagtaat ttgggcccg tttgcactg 
ttagtcata cttttacag ccaataata ttttcattt 
gtgttaacc aagcacagg aaattgttg atttggttg 
tgcaacggc cttgtccta gtgctaatt tccacgtat 
aagaatttt cttgcaaga gaggaattt ttgatttgg 
ttgaaacta aacgaacaa actgcttac ataactatg 
ataacgata ttttggatg aacttgcgt tgtgttagc 
tacggttta gacctgata gattccaaa gggaggcaa 
gatttgatc gcatttttg tcatactgt ctgtacctt 
ccaaattta caaactgga ccatttatt ccctacaaa 
gattttatc aattctcat gaacacaac aataattta 
tttagccgc ctttcacaa ctattccgg gacgtggtt 
cggttttta ttttggttg ttttttgcc gaattgtgt 
ttacatttt atttatgtg aacgctgcg ggatttcac 
cccgagtgg gtcgaatca ctggattcg tggtcgttg 
tatggctat ggatacacc atgggccag tttttccac 
attaaatat ttggtcacg tatggatta gcaacaagt 
tttgcaaaa ttcgataac attaaagtg ccttcttta 
ccggtatgt atcggaaga atacacttg tactccgac 
atgtggaag tatttcgat gtgggcctt tacaatttt 
ttattacga tatatttac atcccctcg atgaacaac 
ggatttgtt cgtagccgg tccttgtct agtttcctt 
tgctttctt tttgtctac gtctggcac ggaaccgat 
aattacata ttaatttgg acgcttttg aattacatt 
ggtgtgata attgagaat tgtagtcac tggtttagt 
gccatttat ttgcaaaac tcgaagttg tgtaaaggt 
ttggggccc aaatggggc cggcgaatc aactgcatg 
gctgcaagc ccccttttg gccgtgtcg gcaatttca 
aatttttat tttttcgcc cggatggaa atcggaaat 
atttttctt aacagattt ttgaatgat aattattgg 
tccaacttc gtactattg gccatttta tacaactgt 
tgtcaggtt tctaccgaa gtaaggagg aaacaatct 
aataaaaac tcaagactg aaagacttg tagcaaaat 
atttatttt atacaattc ttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY