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Gene information
General information
TC010818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6015014..6016716
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010818
    iBeetle screen iB_08703

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010818 RA
      MDYLITIYG CMFLVGVLG NGTLCLTLC CGPGAKTRS 
      PLLFGLIFA DLLVCCLSG PVTAALYVL SNWTQTWKY 
      IALFIQAWP VSASTLLMM AISVDRYLT IKSYRPAGQ 
      VARRRHLLN TAVIITWIS AAVLSGTQF ISKHPWRKS 
      LVITHVLLV YVLPACAVL VSHLGVHAK LTAISLTAR 
      AKHGELPLP MPLLRRPTH VIIVAGISK HGRDRLQAT 
      ETSVEDISQ PPTSTLRSR RRLANSLLW VAIVFAVCW 
      FPYIVCQFC GELGTSPPA TVQRYSLLL GHTHSAFSP 
      ILYWTLNHQ WPQRPCRFR LPVLYRSAS STNEAALGP 
      FHPRLVRPP PIRRRSSHY LY
    CDS sequence
    • >TC010818 RA
      atggattat ttaataacg atatatggg tgcatgttt 
      ttggtgggt gttctcggc aatgggaca ctttgtctg 
      acactgtgt tgcggtccc ggggccaaa actcgcagt 
      cctttgctc tttggtctc attttcgcg gaccttcta 
      gtatgctgc ctgagtggc cctgtcact gcggcactc 
      tacgtcctt tcaaactgg acccaaact tggaaatat 
      atcgctctg tttatccag gcctggcct gtttctgct 
      agcacttta ttgatgatg gctatatct gtcgaccgt 
      tacctcacg attaaaagt tacaggcca gctgggcaa 
      gttgcacgg agaagacac ttgcttaac actgcggtc 
      attatcacc tggatctcc gctgcagta ttatctggt 
      acacaattt atttctaaa catccatgg cggaaaagc 
      ttagttatc acccatgtg ctgttggtg tatgttttg 
      ccagcttgt gcggtgctc gtaagtcac ttaggagtg 
      cacgctaag ctcactgca atttccctg actgcccgc 
      gccaagcac ggcgaacta cccttacca atgcccctc 
      ttgcggaga ccgacccac gttatcatt gttgccggg 
      atttccaag cacggtaga gacaggctc caggcgacc 
      gaaacgagc gtcgaagac atttcccag ccgcccacc 
      agcacgctc cgcagtcgt cgccgtctg gcgaattct 
      ttgctatgg gtggcgatt gttttcgcg gtatgttgg 
      tttccttat atagtgtgc caattttgc ggcgaattg 
      ggaacgtct cctccggca acggtccaa cggtacagc 
      ttacttctt ggccacact cattcggcg ttcagtcca 
      attctttac tggacgctg aaccaccag tggccccag 
      cgaccatgt cgctttcgg ctccctgtc ctttacaga 
      agcgcttcg tcaacgaac gaagccgct cttggtcca 
      tttcatcct agactggta cgtccgcca ccgatccgt 
      cgaagatcc tcacattat ctctactaa
    mRNA sequence
    • >TC010818 RA
      tggggttgc agcttcagg tgggtgttg ccctgtcgt 
      tactgccta gcacatgta atactgcta gctccaata 
      actgagact ttggtattt tccaaaatg aaacagatc 
      cagtgacca acgaatgga ttatttaat aacgatata 
      tgggtgcat gtttttggt gggtgttct cggcaatgg 
      gacactttg tctgacact gtgttgcgg tcccggggc 
      caaaactcg cagtccttt gctctttgg tctcatttt 
      cgcggacct tctagtatg ctgcctgag tggccctgt 
      cactgcggc actctacgt cctttcaaa ctggaccca 
      aacttggaa atatatcgc tctgtttat ccaggcctg 
      gcctgtttc tgctagcac tttattgat gatggctat 
      atctgtcga ccgttacct cacgattaa aagttacag 
      gccagctgg gcaagttgc acggagaag acacttgct 
      taacactgc ggtcattat cacctggat ctccgctgc 
      agtattatc tggtacaca atttatttc taaacatcc 
      atggcggaa aagcttagt tatcaccca tgtgctgtt 
      ggtgtatgt tttgccagc ttgtgcggt gctcgtaag 
      tcacttagg agtgcacgc taagctcac tgcaatttc 
      cctgactgc ccgcgccaa gcacggcga actaccctt 
      accaatgcc cctcttgcg gagaccgac ccacgttat 
      cattgttgc cgggatttc caagcacgg tagagacag 
      gctccaggc gaccgaaac gagcgtcga agacatttc 
      ccagccgcc caccagcac gctccgcag tcgtcgccg 
      tctggcgaa ttctttgct atgggtggc gattgtttt 
      cgcggtatg ttggtttcc ttatatagt gtgccaatt 
      ttgcggcga attgggaac gtctcctcc ggcaacggt 
      ccaacggta cagcttact tcttggcca cactcattc 
      ggcgttcag tccaattct ttactggac gctgaacca 
      ccagtggcc ccagcgacc atgtcgctt tcggctccc 
      tgtccttta cagaagcgc ttcgtcaac gaacgaagc 
      cgctcttgg tccatttca tcctagact ggtacgtcc 
      gccaccgat ccgtcgaag atcctcaca ttatctcta 
      ctaatgact gtctgtgat tactgggcc tcgctgata 
      actacctca cgattcacg atatctact atttctcat 
      ttttggcat aacttcatt atcggacaa ttaatgtta 
      ttgctaata tgcctaagg agacggtat atatgtaaa 
      gaaaagacg atttcataa atatatttt tttatttat 
      agtgaacgt tcaggctct atacatata tactgtaat 
      aaataattg ttgttcatt gttttattg ctgttgtgt 
      tgatcattg ttcaagtgg aacgtgaag aagaatgaa 
      tgtagccga cctgacaac gttattcag catcctcga 
      ctggtactt tacatattt aattatctc taggtaaca 
      aataaaaaa atcaaaaaa at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY