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Gene information
General information
TC010833 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6080960..6082094
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010833
    iBeetle screen iB_08696

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010833 RA
      MDNSNAKNK YQTRQEYVD KLKQWLDEA RLWHGFCAS 
      FPYYTNLQN QTENSTWNV AYLNRHSNR RNLNVPVFQ 
      PGIHEFVIP PLWKRIVAE FLDFLILLL IKMVVTFVI 
      IECFYVLSI ENYRFDSFK KNLQDPKIA MQMSVEILT 
      LELLHRFIV CCYEVYWLK GGSCATPGK KYMGLMVIQ 
      VENITPVPG QSDDVVRVS PCLPLGIQK AIIRAILKN 
      VFLGVMLPM CFTLYVFRF NRTGYDAMS NSLVVECNP 
      QIQFHVQ
    CDS sequence
    • >TC010833 RA
      atggacaat tccaatgca aaaaataag taccagacc 
      cgccaggag tatgtggac aagctgaaa cagtggctg 
      gatgaagct aggttatgg catggattc tgtgcgagc 
      ttcccttac tacacaaat ttgcaaaac cagacagaa 
      aacagtact tggaatgtg gcttattta aaccgacac 
      agtaatagg aggaatctg aacgttcca gttttccag 
      ccaggtatt cacgaattt gtgataccg cccctatgg 
      aaacgaatc gtggccgaa ttcctagat ttcctaatc 
      cttttgtta ataaaaatg gttgtaacg tttgtgata 
      attgaatgt ttttatgtc ctaagcata gaaaactac 
      cggtttgac tcgtttaag aagaaccta caggaccct 
      aaaatcgcc atgcagatg tccgtcgaa atattgact 
      ctggagctg ctccaccgc ttcatagtg tgttgctat 
      gaggtttat tggcttaaa gggggctcg tgtgccacc 
      ccggggaag aaatacatg ggtctcatg gtaatacaa 
      gttgagaat attacccca gtgccgggg cagtcggac 
      gacgttgtg agagtgagc ccgtgtttg ccgctcggc 
      atccaaaag gccatcatt cgggcgatt ttaaaaaac 
      gtgttcctg ggcgtcatg ttgcccatg tgctttact 
      ctgtatgtg tttcggttt aaccgcacc ggttacgac 
      gcaatgagc aacagcttg gttgtagaa tgcaatccg 
      caaattcag tttcacgtt cagtga
    mRNA sequence
    • >TC010833 RA
      gaagtggaa aatgtcacg tcattcgcg tccacctat 
      tatctctgc tccagtggt gggaatcgt ctgatccct 
      ataaattca aatgtgaaa ttttgagtt tttcatcat 
      gtttttcaa tatggacaa ttccaatgc aaaaaataa 
      gtaccagac ccgccagga gtatgtgga caagctgaa 
      acagtggct ggatgaagc taggttatg gcatggatt 
      ctgtgcgag cttccctta ctacacaaa tttgcaaaa 
      ccagacaga aaacagtac ttggaatgt ggcttattt 
      aaaccgaca cagtaatag gaggaatct gaacgttcc 
      agttttcca gccaggtat tcacgaatt tgtgatacc 
      gcccctatg gaaacgaat cgtggccga attcctaga 
      tttcctaat ccttttgtt aataaaaat ggttgtaac 
      gtttgtgat aattgaatg tttttatgt cctaagcat 
      agaaaacta ccggtttga ctcgtttaa gaagaacct 
      acaggaccc taaaatcgc catgcagat gtccgtcga 
      aatattgac tctggagct gctccaccg cttcatagt 
      gtgttgcta tgaggttta ttggcttaa agggggctc 
      gtgtgccac cccggggaa gaaatacat gggtctcat 
      ggtaataca agttgagaa tattacccc agtgccggg 
      gcagtcgga cgacgttgt gagagtgag cccgtgttt 
      gccgctcgg catccaaaa ggccatcat tcgggcgat 
      tttaaaaaa cgtgttcct gggcgtcat gttgcccat 
      gtgctttac tctgtatgt gtttcggtt taaccgcac 
      cggttacga cgcaatgag caacagctt ggttgtaga 
      atgcaatcc gcaaattca gtttcacgt tcagtgaag 
      aagcatttt tcataaaac acttttatt actgctaaa 
      aaattacat atctttctt tagaagtgc aagaatagg 
      taataaaac aaactttgt gga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY