Status
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Gene information
General information
TC033626
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009695,TC009696
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4627279..4631692
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033626
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033626 RA
      MKRVFFSYQ FLATVTSSL AVLSSGIHE AWTSVYIPV 
      LLNGTNTLK ITSVEGSWI TMSMSFGGL IGCVVSCLL 
      INKIGRKKT ILLTFCPNF LSSVVLAFA NSVPVFCTA 
      RVLSGVAFG IAIGVIPHY IGEIADPEI RGSLGTLVT 
      IFSLSGFLF INIVGSYVT IQTSSWISA TIPVLFLLT 
      FIWMPESPY YLVMIGECD QAEQTLAKL KGTRDVFDE 
      FQNLKDVMP VSVITIALT FGLNVWHSF IMSDPTVTI 
      QQGQLRGKI STNIDNKNF YSFQGIPYA KPPTGPLRF 
      KDPQAPEPW TGVKDATTE GSVCYAKHM LFNNIIGSE 
      DCLHLNVYT PELASKNLR PVMVWIHGG AFKSGSSNT 
      DVYGPEYLL TQDVVVVTF NYRLGIFGF LKLDDATLG 
      VPGNAGMKD MVMALKWVR KNISKFGGD PNNVTIFGE 
      SAGAASVHY LVLSPLAKG LFHRAIAQS GTALNSFAR 
      GRSDMVSHI SAELNTKNE REILEKLVQ MPAEQLFEL 
      YEKLVTVCE GLNNAGGKL PFAPVIEKA SQNAFIAEE 
      PLKIIKSGN YNQVPLIFG FTTREGMLI EQMIKPRKP 
      TMPRDFEAA IPYTLEVER GSENSKQIA QKIKDFYYG 
      EPGSEDKLE NFYIIHTDN YFLRDIIFA VKHHAATSK 
      FPVYLYQMS VDSSLNVMK RLSNITAPG VAHGDDIGY 
      LFKTKVSPA LNPGSLEEV SVRRFVRLW TNFARNGNP 
      DEPQWKPVK TNELNYIDI GEKLSAKVN PEPERMKFW 
      EELYKNKPL YSI
    CDS sequence
    • >TC033626 RA
      atgaagcgt gtgtttttt tcgtaccag tttttggcc 
      actgtgacc tcctcgttg gctgtttta tcgtcgggg 
      attcacgaa gcctggact tcggtctac atccccgtt 
      ttgctgaac ggcaccaac actttgaaa ataacaagt 
      gtggaaggg tcctggatt acgatgtcc atgagtttt 
      ggcggattg atcggctgt gttgtgtct tgccttctg 
      attaataaa atcggacgc aaaaaaacg atactcctg 
      acgttttgt cccaatttt ttgtcctcg gtggtcctg 
      gcttttgcc aattcggtg ccggttttt tgcacagct 
      cgggtttta tcgggtgtt gctttcggg attgcaatc 
      ggggttatt ccccattat atcggcgaa atcgcagat 
      ccggaaatt aggggttcg ttgggcaca cttgtgact 
      attttttcg ctgtctggt ttcctcttt ataaacatt 
      gtcggttcg tacgtaacg atacaaaca tcgtcttgg 
      atttcggca acaattcca gttctattc ctactgact 
      tttatttgg atgccggaa agtccgtac tatttggta 
      atgattgga gagtgcgat caggctgaa caaactttg 
      gcaaaactt aagggaaca cgcgatgtt tttgacgag 
      ttccaaaat ttgaaagat gtcatgcca gtttctgtt 
      atcactatt gccctaacc ttcggttta aacgtctgg 
      cacagtttc atcatgtct gatcccacc gtcaccata 
      cagcaggga caactccgg ggaaaaatc tcgaccaac 
      attgacaat aaaaacttc tacagtttc caaggcatt 
      ccatacgcc aaacccccc actggacca ctccggttc 
      aaggaccct caagccccg gaaccatgg accggagtc 
      aaggacgca acgacggaa ggaagcgtg tgttacgcc 
      aaacacatg cttttcaat aacattatc ggatcggag 
      gattgccta cacttgaat gtttacaca ccggaattg 
      gcatcaaaa aatttaaga ccagtcatg gtctggata 
      catggtggc gcctttaaa agcggctca agtaatacg 
      gatgtctac ggtcctgag tatttgcta actcaggat 
      gttgttgtc gttactttc aactacaga ttgggaatt 
      ttcgggttt cttaaactg gatgatgcc acgttaggt 
      gttcccggg aacgcggga atgaaagac atggtaatg 
      gccttgaaa tgggtccgg aaaaacatc agtaaattc 
      ggtggggat ccgaacaat gtgaccatt tttggtgaa 
      agtgctgga gcagcctcg gtgcattac ctagtcttg 
      tcaccacta gccaaaggg ctgtttcac agggccatt 
      gcacagagt gggactgcg ttaaattct tttgcaagg 
      gggcgcagt gacatggtt tcgcatatc tcggctgag 
      ttgaacacc aaaaatgag agggaaatt ttggaaaag 
      ttggtgcaa atgccagcc gaacaactg tttgaactg 
      tacgagaaa ttagtaact gtgtgtgag ggtttgaac 
      aatgccggt ggtaaacta cccttcgct ccggtgatt 
      gaaaaggcg tctcaaaat gcgttcatt gccgaagaa 
      ccgctcaaa atcataaaa tcggggaat tacaaccaa 
      gtgcctcta atattcggg tttacgaca agagaggga 
      atgttgatt gagcaaatg ataaagccg agaaagccc 
      accatgccg cgggatttt gaggcagcg attccttac 
      actctggag gttgaacgg ggttcggaa aattcgaaa 
      caaatcgct cagaagatc aaagatttt tattacgga 
      gagccaggc tccgaagac aagcttgaa aatttctac 
      attattcat accgacaac tatttcctg agagatatt 
      atttttgcg gtcaaacac catgccgct acgtccaaa 
      ttcccggtt tatttgtac caaatgtcg gtagattca 
      agtttgaat gtcatgaaa agactttca aacataact 
      gcgccaggt gtagctcac ggtgacgac atcggctac 
      ctcttcaaa accaaagtc tcgccagct ttaaacccc 
      ggaagtttg gaggaagta tcagtgaga cgtttcgtc 
      agactttgg acgaacttc gccagaaac ggcaatcca 
      gacgaaccg cagtggaaa ccggtgaaa acgaacgaa 
      ctcaattac atcgacatt ggggagaaa ttaagtgcg 
      aaagttaac cccgagccc gaaagaatg aaattctgg 
      gaagaactt tacaaaaat aaaccactg tattcaatt 
      taa
    mRNA sequence
    • >TC033626 RA
      agtgagcca cgatgaagc gtgtgtttt tttcgtacc 
      agtttttgg ccactgtga cctcctcgt tggctgttt 
      tatcgtcgg ggattcacg aagcctgga cttcggtct 
      acatccccg ttttgctga acggcacca acactttga 
      aaataacaa gtgtggaag ggtcctgga ttacgatgt 
      ccatgagtt ttggcggat tgatcggct gtgttgtgt 
      cttgccttc tgattaata aaatcggac gcaaaaaaa 
      cgatactcc tgacgtttt gtcccaatt ttttgtcct 
      cggtggtcc tggcttttg ccaattcgg tgccggttt 
      tttgcacag ctcgggttt tatcgggtg ttgctttcg 
      ggattgcaa tcggggtta ttccccatt atatcggcg 
      aaatcgcag atccggaaa ttaggggtt cgttgggca 
      cacttgtga ctatttttt cgctgtctg gtttcctct 
      ttataaaca ttgtcggtt cgtacgtaa cgatacaaa 
      catcgtctt ggatttcgg caacaattc cagttctat 
      tcctactga cttttattt ggatgccgg aaagtccgt 
      actatttgg taatgattg gagagtgcg atcaggctg 
      aacaaactt tggcaaaac ttaagggaa cacgcgatg 
      tttttgacg agttccaaa atttgaaag atgtcatgc 
      cagtttctg ttatcacta ttgccctaa ccttcggtt 
      taaacgtct ggcacagtt tcatcatgt ctgatccca 
      ccgtcacca tacagcagg gacaactcc ggggaaaaa 
      tctcgacca acattgaca ataaaaact tctacagtt 
      tccaaggca ttccatacg ccaaacccc ccactggac 
      cactccggt tcaaggacc ctcaagccc cggaaccat 
      ggaccggag tcaaggacg caacgacgg aaggaagcg 
      tgtgttacg ccaaacaca tgcttttca ataacatta 
      tcggatcgg aggattgcc tacacttga atgtttaca 
      caccggaat tggcatcaa aaaatttaa gaccagtca 
      tggtctgga tacatggtg gcgccttta aaagcggct 
      caagtaata cggatgtct acggtcctg agtatttgc 
      taactcagg atgttgttg tcgttactt tcaactaca 
      gattgggaa ttttcgggt ttcttaaac tggatgatg 
      ccacgttag gtgttcccg ggaacgcgg gaatgaaag 
      acatggtaa tggccttga aatgggtcc ggaaaaaca 
      tcagtaaat tcggtgggg atccgaaca atgtgacca 
      tttttggtg aaagtgctg gagcagcct cggtgcatt 
      acctagtct tgtcaccac tagccaaag ggctgtttc 
      acagggcca ttgcacaga gtgggactg cgttaaatt 
      cttttgcaa gggggcgca gtgacatgg tttcgcata 
      tctcggctg agttgaaca ccaaaaatg agagggaaa 
      ttttggaaa agttggtgc aaatgccag ccgaacaac 
      tgtttgaac tgtacgaga aattagtaa ctgtgtgtg 
      agggtttga acaatgccg gtggtaaac tacccttcg 
      ctccggtga ttgaaaagg cgtctcaaa atgcgttca 
      ttgccgaag aaccgctca aaatcataa aatcgggga 
      attacaacc aagtgcctc taatattcg ggtttacga 
      caagagagg gaatgttga ttgagcaaa tgataaagc 
      cgagaaagc ccaccatgc cgcgggatt ttgaggcag 
      cgattcctt acactctgg aggttgaac ggggttcgg 
      aaaattcga aacaaatcg ctcagaaga tcaaagatt 
      tttattacg gagagccag gctccgaag acaagcttg 
      aaaatttct acattattc ataccgaca actatttcc 
      tgagagata ttatttttg cggtcaaac accatgccg 
      ctacgtcca aattcccgg tttatttgt accaaatgt 
      cggtagatt caagtttga atgtcatga aaagacttt 
      caaacataa ctgcgccag gtgtagctc acggtgacg 
      acatcggct acctcttca aaaccaaag tctcgccag 
      ctttaaacc ccggaagtt tggaggaag tatcagtga 
      gacgtttcg tcagacttt ggacgaact tcgccagaa 
      acggcaatc cagacgaac cgcagtgga aaccggtga 
      aaacgaacg aactcaatt acatcgaca ttggggaga 
      aattaagtg cgaaagtta accccgagc ccgaaagaa 
      tgaaattct gggaagaac tttacaaaa ataaaccac 
      tgtattcaa tttaatgta atttcaaaa attctaata 
      aaaataaaa ttgttat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY