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Gene information
General information
TC033624
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009138,TC009137
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4398590..4429931
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033624
    iBeetle screen iB_08694

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033624 RA
      MERPGEYGD DPPPGLEAM WGMEAAECD CPGPPPEFL 
      LPPPPRPPI LQPSDPKYC TEDPLPIET CDALPLIDA 
      SYRSSPSLQ TSAVIVLCS ALGLFVIIA IILVWKHKR 
      KVQNFLPCK TSPQNNCDG PAAPSSLYE DLHDVIPRP 
      TPTHHHHSA GGHIVAPSI EMIDVKNNI HYPSGYPIS 
      QHPPVFLCS SPGPDPYCS QDLYNPVYE ELSDADSEL 
      APAPHSEDE FAEDELSLG GDMDRRRLD IPPPPYRPE 
      PPPPRRENA RFRRPHEPL PPIPTNKRH RSLDRRRQE 
      FHEGMLLDA LLQLYPRVG SVEPPHRRQ IPSITQRIQ 
      CLSPTPGPY ETVPVLGHL ATFRPVPKP YSQDSAFGS 
      DSGYSNHTS GTRGSSRGR RDPRRLSQL SADLALT
    CDS sequence
    • >TC033624 RA
      atggaaagg cctggagag tacggagac gatccaccg 
      ccaggtctg gaggccatg tggggaatg gaagcggct 
      gaatgcgac tgtcccggg ccgcccccg gaattcctc 
      cttccgccg cccccacgg ccgcccatc ctccagccc 
      tccgacccc aagtactgc acggaagac cccctgcct 
      atcgagacg tgtgatgcg ctaccgctg atcgacgca 
      agctaccgc agtagtcca tctctccag acatcggct 
      gtgatagtg ctgtgttcc gctcttggt ctgttcgtt 
      atcatcgcc attatcctc gtgtggaaa cataaaaga 
      aaagtacaa aatttccta ccttgtaaa acttcgccc 
      caaaacaac tgtgatggt cccgctgct ccctccagc 
      ctttacgag gacctccac gacgtcatt ccacgacca 
      accccaacg catcaccac cactcagcc ggtggacat 
      atcgttgcc ccttccata gagatgatc gacgtaaaa 
      aacaacatc cactacccc agcggctac cccatctcg 
      caacacccc cccgtattc ctgtgttcg tccccaggc 
      ccggacccc tactgctcc caggacctc tacaacccc 
      gtgtacgaa gagctcagc gacgccgac agcgagctg 
      gccccagcc ccccacagc gaggacgag ttcgccgaa 
      gacgagctg agcctcggg ggcgacatg gaccgccgc 
      cgcctcgac atcccgccg ccgccgtac cggcccgag 
      ccgccgccg ccgcgccgc gagaacgcc cgcttccgc 
      cgcccccac gagccgctg ccgcccatt cccaccaac 
      aagcgccac cgaagcctc gaccggcgg cggcaggag 
      ttccacgag ggcatgctg ctcgacgcc ctgctgcag 
      ctgtacccg cgcgtgggc agcgtggag ccgccccac 
      cgccgccag atccccagc atcacgcag cggatccag 
      tgcttgtcc cccacgccg gggccctac gagacagtg 
      cctgtgttg ggacactta gcgactttt cggcccgtg 
      cctaaaccc tactcgcag gactcggcc ttcggctcc 
      gattcgggg tacagtaac cacacgagt ggcacgagg 
      ggctccagc aggggccgc agggacccc aggaggctc 
      tcgcagttg agtgcggac ctggcgctt acgtaa
    mRNA sequence
    • >TC033624 RA
      aaagcatgc agctgtttg aaataagct cagcatgta 
      gtttacggc agtttattt tcggccttc gatggttgg 
      aggtgtgtg tcttggtcg gtctttgtg cgcgacaac 
      ctggattac agtggcctt tgaggaatt tttctcgag 
      ctggatttt ctctctttg tgggattta tgcgtgctt 
      atttagtga tggtgatgt gggcgataa caagtgata 
      cgagggcat gttgaagta atcgggtga ggtctatgg 
      aaaggcctg gagagtacg gagacgatc caccgccag 
      gtctggagg ccatgtggg gaatggaag cggctgaat 
      gcgactgtc ccgggccgc ccccggaat tcctccttc 
      cgccgcccc cacggccgc ccatcctcc agccctccg 
      accccaagt actgcacgg aagaccccc tgcctatcg 
      agacgtgtg atgcgctac cgctgatcg acgcaagct 
      accgcagta gtccatctc tccagacat cggctgtga 
      tagtgctgt gttccgctc ttggtctgt tcgttatca 
      tcgccatta tcctcgtgt ggaaacata aaagaaaag 
      tacaaaatt tcctacctt gtaaaactt cgccccaaa 
      acaactgtg atggtcccg ctgctccct ccagccttt 
      acgaggacc tccacgacg tcattccac gaccaaccc 
      caacgcatc accaccact cagccggtg gacatatcg 
      ttgcccctt ccatagaga tgatcgacg taaaaaaca 
      acatccact accccagcg gctacccca tctcgcaac 
      acccccccg tattcctgt gttcgtccc caggcccgg 
      acccctact gctcccagg acctctaca accccgtgt 
      acgaagagc tcagcgacg ccgacagcg agctggccc 
      cagcccccc acagcgagg acgagttcg ccgaagacg 
      agctgagcc tcgggggcg acatggacc gccgccgcc 
      tcgacatcc cgccgccgc cgtaccggc ccgagccgc 
      cgccgccgc gccgcgaga acgcccgct tccgccgcc 
      cccacgagc cgctgccgc ccattccca ccaacaagc 
      gccaccgaa gcctcgacc ggcggcggc aggagttcc 
      acgagggca tgctgctcg acgccctgc tgcagctgt 
      acccgcgcg tgggcagcg tggagccgc cccaccgcc 
      gccagatcc ccagcatca cgcagcgga tccagtgct 
      tgtccccca cgccggggc cctacgaga cagtgcctg 
      tgttgggac acttagcga cttttcggc ccgtgccta 
      aaccctact cgcaggact cggccttcg gctccgatt 
      cggggtaca gtaaccaca cgagtggca cgaggggct 
      ccagcaggg gccgcaggg accccagga ggctctcgc 
      agttgagtg cggacctgg cgcttacgt aaagtgatg 
      tgcaatgta cttaggact ttgttttta ctgccaaag 
      gaagggtgg tgagcgagg gacaagtga atttaacgg 
      ttttgcgga tgtacaaat ataattaaa aaaaaatat 
      tgcaaaagt tcagttttt gaatatttt ggaaaaata 
      ttagataat ttcattcat tgacacagg gtgtttcag 
      agtgttttt aagaaacat tatttttgt taggttcta 
      ctttttaca tcactcagt gaataatac atttttggt 
      aagagttaa acatacttt ttcaaataa gagcgattg 
      gtattgaaa aacaatctc agtcaaaca aaataagct 
      gaaaaatta aacttgaat aattttttg ttattttct 
      acattgatt ataatataa tgcatgggc tttttgtca 
      tttttaata ttaaaaaaa atcactttt taaaaccac 
      cgaaatttc tacactaaa aatttatta caaaagaac 
      atttttgcc cacgtttgt aaaaatgaa ggttttttt 
      ttaataaat gcaaatctt gttggaaaa tttttcacg 
      tctgtgatg gttagtcga gtgaactgt gtcacaaaa 
      tttaacaaa gttatcaca ctttttttg taaaagtta 
      aacattttt ttcaaggag caattgtat tgaaaaaca 
      agccgaatc gaacaacac tctttttta ataaaccaa 
      taacaaaag ttttttcgg tttttatta aaatatttt 
      acaaaatca tttatttac gtaataaaa gacagaaaa 
      gaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY