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Gene information
General information
TC015939 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4116331..4120198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015939
    iBeetle screen iB_08685

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015939 RA
      MTTPEWISN NLFQKALNN YFNEDLQLV TFTSTNAVP 
      EGDNYTSDL FRSTLIYIN PKKKTKHSL SVIVKCGND 
      DGGVKTDLA KKLHLFDKE IEMFTRTFP AIYEILGEH 
      YTLSSKCLY ACNDPHSVI VLEDLTTLN FEMLPRHIG 
      FDLEHCCKV VEKMAQMHA ASVIMYEND PSLVKCYAE 
      GLYADNEMI REWVSAGYG ALSDACSRW PGFEKYGYK 
      LSALGDEAL ERGFKATKR RLGGFNVLN HGDLWVNNM 
      MFSYHQDGK LKDMRFVDF QMNIFTSPA IDLHYFIAT 
      STKVEVKLE CIEIILDHY YAQLLANLA RLQYSLERV 
      PTREQFKKD YNARAFYGL MGAATVLAF VKASSRKDA 
      SFESVMRDG SVDGFRYHA YNNERYRKH MEHLLPYYD 
      SFGILD
    CDS sequence
    • >TC015939 RA
      atgacaacg ccagaatgg atctccaac aaccttttt 
      caaaaagct ctcaacaac tatttcaac gaagacctc 
      caacttgtc acgttcact agcaccaac gccgtacca 
      gagggcgac aactacacc agtgacctg ttccggtcc 
      acccttatc tacatcaac cccaaaaaa aaaaccaaa 
      cactccctg tctgttatt gtcaagtgt gggaacgac 
      gacggcggt gttaagaca gacctggcc aaaaaactg 
      cacctcttt gacaaggaa atcgaaatg tttacgcgc 
      acttttccg gcaatttac gaaattttg ggtgagcat 
      tacacgctt tcgtccaag tgtctctat gcctgtaat 
      gacccccat tcggtgatc gtgcttgag gatttaact 
      actctaaat tttgaaatg ttgccgaga catattggc 
      tttgacctt gaacattgc tgcaaagtt gtggaaaaa 
      atggcacaa atgcatgca gcttcggtt attatgtat 
      gaaaatgac ccttctttg gttaaatgt tacgcggag 
      gggttgtat gccgataac gaaatgata agggagtgg 
      gtaagcgcc ggctacggc gccttaagc gacgcttgt 
      agccgctgg ccaggtttc gaaaagtac ggctacaag 
      ctgtcagcc cttggcgac gaggccctc gagcgcggc 
      ttcaaagcc accaagaga cgcctaggc ggcttcaat 
      gtcctcaac catggcgac ttgtgggtg aataacatg 
      atgttttcc taccaccaa gacggcaaa ctgaaggac 
      atgcgattt gtcgacttc caaatgaac attttcacg 
      agccctgcg atcgatctg cactatttc atcgcaacg 
      agcaccaaa gtcgaggtc aagcttgag tgcattgag 
      atcattttg gaccactat tacgcccag ttgctggca 
      aatctggcc agactccag tactcgctg gagagggtc 
      cccacgcgg gagcagttc aagaaggac tacaacgca 
      cgggcgttc tacggcctc atgggggct gccacggtc 
      ctcgctttc gtcaaagct tcgagccgg aaggacgcc 
      tcgttcgaa agcgtgatg cgggatggg agcgtggac 
      ggctttcgc taccatgct tacaataac gaacggtac 
      aggaagcac atggagcac ctgttgccc tattatgac 
      agtttcggg atcctagac taa
    mRNA sequence
    • >TC015939 RA
      gtctaattg aaaaaagcc gacgttcga aattacgga 
      ttcgctaca ttgttatta cttttgcat gctttattt 
      gcctgatgt ataaaagag gaagtatcg gaactggtc 
      cataagtga cgtacagtc gttgaaaaa ggtaatttc 
      tagacgggt tttggtgtt ggaacctaa ctgtagtca 
      gatggggtt ggataaact aatcctgaa ggtgctcct 
      catgtgtgc cctgttcct aactgtcta tacccgtag 
      atgtagaac tcgtaacag aactagaat ttgtgttaa 
      ggatgagcc agcgtcgat ttttcttga caaattatt 
      ctcctcata aaaatgaca acgccagaa tggatctcc 
      aacaacctt tttcaaaaa gctctcaac aactatttc 
      aacgaagac ctccaactt gtcacgttc actagcacc 
      aacgccgta ccagagggc gacaactac accagtgac 
      ctgttccgg tccaccctt atctacatc aaccccaaa 
      aaaaaaacc aaacactcc ctgtctgtt attgtcaag 
      tgtgggaac gacgacggc ggtgttaag acagacctg 
      gccaaaaaa ctgcacctc tttgacaag gaaatcgaa 
      atgtttacg cgcactttt ccggcaatt tacgaaatt 
      ttgggtgag cattacacg ctttcgtcc aagtgtctc 
      tatgcctgt aatgacccc cattcggtg atcgtgctt 
      gaggattta actactcta aattttgaa atgttgccg 
      agacatatt ggctttgac cttgaacat tgctgcaaa 
      gttgtggaa aaaatggca caaatgcat gcagcttcg 
      gttattatg tatgaaaat gacccttct ttggttaaa 
      tgttacgcg gaggggttg tatgccgat aacgaaatg 
      ataagggag tgggtaagc gccggctac ggcgcctta 
      agcgacgct tgtagccgc tggccaggt ttcgaaaag 
      tacggctac aagctgtca gcccttggc gacgaggcc 
      ctcgagcgc ggcttcaaa gccaccaag agacgccta 
      ggcggcttc aatgtcctc aaccatggc gacttgtgg 
      gtgaataac atgatgttt tcctaccac caagacggc 
      aaactgaag gacatgcga tttgtcgac ttccaaatg 
      aacattttc acgagccct gcgatcgat ctgcactat 
      ttcatcgca acgagcacc aaagtcgag gtcaagctt 
      gagtgcatt gagatcatt ttggaccac tattacgcc 
      cagttgctg gcaaatctg gccagactc cagtactcg 
      ctggagagg gtccccacg cgggagcag ttcaagaag 
      gactacaac gcacgggcg ttctacggc ctcatgggg 
      gctgccacg gtcctcgct ttcgtcaaa gcttcgagc 
      cggaaggac gcctcgttc gaaagcgtg atgcgggat 
      gggagcgtg gacggcttt cgctaccat gcttacaat 
      aacgaacgg tacaggaag cacatggag cacctgttg 
      ccctattat gacagtttc gggatccta gactaattc 
      aataaataa tgcaaattg tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY