Status
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Gene information
General information
TC015915 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4151930..4156467
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015915
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08683

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015915 RA
      MSDKSVQVA LRIRPLVPS EISKGCKNV FEVYPSLNQ 
      VKIKNTDKA FTYNYVFEP STTQDYVYE TCVKGMIEN 
      LFNGYNVTV LAYGQTGSG KTHTMGTTY NGEGEMGVI 
      PRAITEIFN TVRENFVYD VSVTVSFVE LYQETLYDL 
      LAEKSRDQC ILDIREDPS KGVVIPGLT QVQVDSTQS 
      VFQALIRGS SSRATGATN MNAQSSRSH AIFSVNMTM 
      INKKDGNEK TAKFHLVDL AGSERPKKT GAVGTTFKE 
      GVNINKGLL VLGNVISAL ADEKQQHGY ISYRDSNLT 
      RLLKDSLGG NSITLMIAC VSPADYNID ESISTLRYA 
      DRARRIQNK PIVNQDPKA AEINLLKKT IQDLKLQIV 
      GQGGPAVSS NELTSLREE NVQLHTRIR DLTVQLSST 
      LHDNTGLLE KLMILQNAN ENLHKKIQE LKQEYDLTL 
      DNINLTFTQ NDSDGFKKN LAKLQQIQE QFTVLDYEQ 
      KKTEDEIFH HETNIDNIR RSQEGKYQN VDEMSKDQE 
      NYTKQQMAL NTELQELTK QLAMKEHLA RQITTNTQY 
      LVDYEGLKE NENKILQLQ KEKDELLQQ LKNAHSNGP 
      SSKIAEQRR KRVQELENQ LQELNRKVQ EQARLIKLK 
      EKDVTKINQ LNNEIQQMK QTRVKLIRK MREESERFR 
      TWKIKRERD IAKLKQEDR KKQTKIVRM EAMHNKQQN 
      VLRRKVEEA VALNKRLKD ALALRKATQ DAKNSGKNE 
      KIGCWMRQE FDVHMHLIE AEATLKGLL DDRATLQQQ 
      LDKLRDDPE NLDESQVQE IESDIEMRS VQIQDLQQK 
      LLDSEEENK KSKFDNIQT MGEAKFAIK ILLEQAADI 
      KASEVSAKN KMIETQAAL GDFKHKCEN LEKVVKIFE 
      LKNNQDLAQ LQKDYEEKI AVLLRQLRG VEVQDGSEE 
      LKQRCAIQS EQIENLERK NQELMEKLH ETQQQAEEL 
      KEKIDTISQ LAKESQIRE AVAELNTPV ENMRRKKIK 
      IKREFDSTF TMENQENFD VSMEELDDI ANDPDWKRT 
      PIGKWVLEN KRRTNAGVE NMALKRTSD GGCMCVKSN 
      CSKRCGCKK LNRSCTVGC RCSSTNCTN RDKIAETTG 
      SLDEKKRRM VNQNLFANN
    CDS sequence
    • >TC015915 RA
      atgtccgac aaatctgta caagtagcc ctcagaata 
      aggcccctt gtcccatcc gagatctcc aaaggttgc 
      aaaaacgtg tttgaggtg taccccagc ctcaatcaa 
      gtcaaaatt aaaaacacc gacaaagct ttcacatac 
      aattatgtt tttgaacca agcacaaca caagactac 
      gtttacgag acctgtgtt aaaggcatg atcgaaaat 
      cttttcaat ggctataac gtcacggtc ctggcatac 
      ggacaaacc ggctctggc aaaacacac accatgggg 
      accacgtac aacggggag ggcgaaatg ggggttatc 
      cccagggca attacggaa atttttaat acagtgagg 
      gagaatttc gtgtatgat gtaagtgtg accgtgtcg 
      ttcgtcgaa ctctatcag gagacgctt tacgactta 
      ttggccgag aaaagtcga gatcagtgc atactggac 
      attagagag gacccaagt aagggtgtg gtaatacct 
      ggcttgacg caagtgcaa gtggactca actcaaagc 
      gtttttcaa gctttgatc agggggtcg agcagtcgc 
      gccacgggg gccaccaat atgaatgcg cagagttcg 
      aggagtcat gcgattttt tcagtgaat atgaccatg 
      atcaacaaa aaggacgga aatgaaaag actgcaaag 
      ttccatttg gtcgatttg gccggttca gaacgaccc 
      aaaaagacg ggggctgtt ggcacaaca tttaaagag 
      ggcgttaac ataaacaaa ggacttcta gtactaggg 
      aacgtgatt tcggcctta gctgatgaa aaacaacaa 
      cacggttat atctcttat cgagacagc aacttaact 
      cgcctcctg aaagactct ttaggcgga aacagcata 
      actttgatg attgcctgt gttagcccc gctgattac 
      aacatagac gaatcaatt tcgacgtta cgttacgcc 
      gatcgggct cgaagaatc caaaataaa ccaatcgta 
      aatcaggac ccgaaagca gccgaaatc aatttgtta 
      aagaaaaca attcaggac ttgaaactg caaattgtg 
      ggtcagggt ggacctgcc gtttcgtcc aatgaactt 
      acaagtttg agggaggaa aacgtccag ttgcacacg 
      agaattcgt gaccttacc gtacagtta tcgtccact 
      ttgcacgac aataccggc cttttggaa aaattaatg 
      attttgcaa aatgccaac gaaaatctc cataaaaaa 
      attcaagaa ctgaaacag gagtacgat ctcacgctc 
      gacaacatc aacttgact ttcacgcaa aatgattcg 
      gacgggttt aagaagaat ttggcaaag ttgcaacaa 
      atccaagaa cagttcact gtgcttgac tacgaacag 
      aagaagact gaagacgag atattccac catgagaca 
      aacattgat aatattcgg cgaagtcag gaaggtaaa 
      taccaaaat gtggacgaa atgagtaaa gaccaggaa 
      aattatacc aagcaacaa atggcactt aacaccgaa 
      ctgcaagag ttgacgaaa cagttagcc atgaaggaa 
      catttggcg cgacaaata accacaaac acgcagtat 
      ctggtcgat tacgagggc ttgaaagaa aacgaaaat 
      aaaatcctc caactgcag aaggaaaag gacgaattg 
      ttgcaacaa ttaaagaac gcacactcg aacgggccg 
      tcgtcgaaa atcgccgaa cagcggcgg aaacgcgtc 
      caagaactg gagaatcag ttgcaagaa ctaaaccgg 
      aaagtccaa gaacaggcg cgtttgatc aaactgaag 
      gagaaggac gtgacaaaa atcaaccaa ctgaacaac 
      gaaatccag caaatgaaa caaactcgc gtcaagttg 
      attcgcaaa atgcgcgaa gaatcggag cgtttccgc 
      acgtggaaa atcaagcga gagcgcgac atcgccaaa 
      cttaagcag gaagaccgc aaaaaacag accaaaatc 
      gtgcgaatg gaagcaatg cacaacaag caacagaac 
      gtcctcaga cggaaagtc gaggaggct gtggcgttg 
      aacaagcgc cttaaggac gccctggcc ttgcgcaaa 
      gccacccaa gacgcgaaa aattcagga aagaacgaa 
      aaaatcggc tgttggatg aggcaagag ttcgacgtc 
      cacatgcat ctaattgag gccgaagcc actttgaag 
      ggacttttg gacgatcgg gcaacgttg cagcaacag 
      ttggacaaa ctgagggac gatcccgaa aatctggac 
      gagtcacaa gtgcaagaa atcgagagt gacatcgaa 
      atgcgttcg gtgcaaatc caggacttg caacagaag 
      ttgctagac tcggaagaa gagaataaa aaatcaaag 
      ttcgataat atccaaacg atgggcgag gcgaaattt 
      gccattaaa atcctcctg gagcaagcg gccgacatc 
      aaggcgagc gaagtgagt gccaagaac aaaatgatc 
      gagacgcaa gcggctctg ggcgatttt aagcacaag 
      tgtgagaat ttggagaag gttgtgaag attttcgag 
      ttgaagaac aaccaagat ttggcacag ttgcaaaaa 
      gactacgag gagaaaatt gctgtgttg ttgcggcaa 
      ctgcgcggt gttgaggtg caggacggg agcgaggag 
      ttgaaacag aggtgtgcg attcaaagc gaacagatt 
      gagaatttg gagcggaaa aaccaagag ttgatggaa 
      aagttgcac gagacgcag caacaagcc gaagaattg 
      aaggaaaaa atcgacact atttcgcag ttggctaaa 
      gaatcgcaa attagggaa gcagttgcc gaacttaac 
      acgccagtc gagaacatg aggcggaaa aaaattaag 
      attaagcga gagtttgat tcgacgttc actatggaa 
      aatcaggag aatttcgat gttagtatg gaagaactg 
      gacgatatt gcaaatgat ccagattgg aagaggacg 
      ccgattggc aagtgggtg cttgaaaat aaaaggagg 
      acgaatgcg ggtgtggaa aatatggca ttgaaaagg 
      acatcagat ggtggttgt atgtgtgtt aaaagtaac 
      tgttcgaaa agatgtggg tgcaagaaa ctgaatcga 
      agttgtaca gtggggtgc aggtgctcc agcacgaat 
      tgtacaaat agagataag attgctgaa actacggga 
      tcattagac gaaaagaaa agaagaatg gtgaaccag 
      aatttattt gcaaataac taa
    mRNA sequence
    • >TC015915 RA
      aataaatag accctgatt atttcattt caaatactc 
      agattttgc cgcgtcacc tcccctcac ccgattggt 
      caattcaat tttcacctt cccaacggt tttcaggtg 
      tcttaatag ttttaaaag ttgactgac aatcgtggc 
      gttttttgg ttttaactt tgtgatagt agtatttat 
      aagtgtata tatttgttt cttatatta agacaagat 
      gtccgacaa atctgtaca agtagccct cagaataag 
      gccccttgt cccatccga gatctccaa aggttgcaa 
      aaacgtgtt tgaggtgta ccccagcct caatcaagt 
      caaaattaa aaacaccga caaagcttt cacatacaa 
      ttatgtttt tgaaccaag cacaacaca agactacgt 
      ttacgagac ctgtgttaa aggcatgat cgaaaatct 
      tttcaatgg ctataacgt cacggtcct ggcatacgg 
      acaaaccgg ctctggcaa aacacacac catggggac 
      cacgtacaa cggggaggg cgaaatggg ggttatccc 
      cagggcaat tacggaaat ttttaatac agtgaggga 
      gaatttcgt gtatgatgt aagtgtgac cgtgtcgtt 
      cgtcgaact ctatcagga gacgcttta cgacttatt 
      ggccgagaa aagtcgaga tcagtgcat actggacat 
      tagagagga cccaagtaa gggtgtggt aatacctgg 
      cttgacgca agtgcaagt ggactcaac tcaaagcgt 
      ttttcaagc tttgatcag ggggtcgag cagtcgcgc 
      cacgggggc caccaatat gaatgcgca gagttcgag 
      gagtcatgc gattttttc agtgaatat gaccatgat 
      caacaaaaa ggacggaaa tgaaaagac tgcaaagtt 
      ccatttggt cgatttggc cggttcaga acgacccaa 
      aaagacggg ggctgttgg cacaacatt taaagaggg 
      cgttaacat aaacaaagg acttctagt actagggaa 
      cgtgatttc ggccttagc tgatgaaaa acaacaaca 
      cggttatat ctcttatcg agacagcaa cttaactcg 
      cctcctgaa agactcttt aggcggaaa cagcataac 
      tttgatgat tgcctgtgt tagccccgc tgattacaa 
      catagacga atcaatttc gacgttacg ttacgccga 
      tcgggctcg aagaatcca aaataaacc aatcgtaaa 
      tcaggaccc gaaagcagc cgaaatcaa tttgttaaa 
      gaaaacaat tcaggactt gaaactgca aattgtggg 
      tcagggtgg acctgccgt ttcgtccaa tgaacttac 
      aagtttgag ggaggaaaa cgtccagtt gcacacgag 
      aattcgtga ccttaccgt acagttatc gtccacttt 
      gcacgacaa taccggcct tttggaaaa attaatgat 
      tttgcaaaa tgccaacga aaatctcca taaaaaaat 
      tcaagaact gaaacagga gtacgatct cacgctcga 
      caacatcaa cttgacttt cacgcaaaa tgattcgga 
      cgggtttaa gaagaattt ggcaaagtt gcaacaaat 
      ccaagaaca gttcactgt gcttgacta cgaacagaa 
      gaagactga agacgagat attccacca tgagacaaa 
      cattgataa tattcggcg aagtcagga aggtaaata 
      ccaaaatgt ggacgaaat gagtaaaga ccaggaaaa 
      ttataccaa gcaacaaat ggcacttaa caccgaact 
      gcaagagtt gacgaaaca gttagccat gaaggaaca 
      tttggcgcg acaaataac cacaaacac gcagtatct 
      ggtcgatta cgagggctt gaaagaaaa cgaaaataa 
      aatcctcca actgcagaa ggaaaagga cgaattgtt 
      gcaacaatt aaagaacgc acactcgaa cgggccgtc 
      gtcgaaaat cgccgaaca gcggcggaa acgcgtcca 
      agaactgga gaatcagtt gcaagaact aaaccggaa 
      agtccaaga acaggcgcg tttgatcaa actgaagga 
      gaaggacgt gacaaaaat caaccaact gaacaacga 
      aatccagca aatgaaaca aactcgcgt caagttgat 
      tcgcaaaat gcgcgaaga atcggagcg tttccgcac 
      gtggaaaat caagcgaga gcgcgacat cgccaaact 
      taagcagga agaccgcaa aaaacagac caaaatcgt 
      gcgaatgga agcaatgca caacaagca acagaacgt 
      cctcagacg gaaagtcga ggaggctgt ggcgttgaa 
      caagcgcct taaggacgc cctggcctt gcgcaaagc 
      cacccaaga cgcgaaaaa ttcaggaaa gaacgaaaa 
      aatcggctg ttggatgag gcaagagtt cgacgtcca 
      catgcatct aattgaggc cgaagccac tttgaaggg 
      acttttgga cgatcgggc aacgttgca gcaacagtt 
      ggacaaact gagggacga tcccgaaaa tctggacga 
      gtcacaagt gcaagaaat cgagagtga catcgaaat 
      gcgttcggt gcaaatcca ggacttgca acagaagtt 
      gctagactc ggaagaaga gaataaaaa atcaaagtt 
      cgataatat ccaaacgat gggcgaggc gaaatttgc 
      cattaaaat cctcctgga gcaagcggc cgacatcaa 
      ggcgagcga agtgagtgc caagaacaa aatgatcga 
      gacgcaagc ggctctggg cgattttaa gcacaagtg 
      tgagaattt ggagaaggt tgtgaagat tttcgagtt 
      gaagaacaa ccaagattt ggcacagtt gcaaaaaga 
      ctacgagga gaaaattgc tgtgttgtt gcggcaact 
      gcgcggtgt tgaggtgca ggacgggag cgaggagtt 
      gaaacagag gtgtgcgat tcaaagcga acagattga 
      gaatttgga gcggaaaaa ccaagagtt gatggaaaa 
      gttgcacga gacgcagca acaagccga agaattgaa 
      ggaaaaaat cgacactat ttcgcagtt ggctaaaga 
      atcgcaaat tagggaagc agttgccga acttaacac 
      gccagtcga gaacatgag gcggaaaaa aattaagat 
      taagcgaga gtttgattc gacgttcac tatggaaaa 
      tcaggagaa tttcgatgt tagtatgga agaactgga 
      cgatattgc aaatgatcc agattggaa gaggacgcc 
      gattggcaa gtgggtgct tgaaaataa aaggaggac 
      gaatgcggg tgtggaaaa tatggcatt gaaaaggac 
      atcagatgg tggttgtat gtgtgttaa aagtaactg 
      ttcgaaaag atgtgggtg caagaaact gaatcgaag 
      ttgtacagt ggggtgcag gtgctccag cacgaattg 
      tacaaatag agataagat tgctgaaac tacgggatc 
      attagacga aaagaaaag aagaatggt gaaccagaa 
      tttatttgc aaataacta agagtctga ggcctaatt 
      ttatgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY