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Gene information
General information
TC005187 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4568119..4569831
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005187
    iBeetle screen iB_08674

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005187 RA
      MAKRPLSDY TQFQRVVSQ ARSIVALTG AGVSAESGI 
PVFRGAGGL WRTHRATDL ATPTAFRAN PALVWEFYH 
YRRDVAFNS QPNNAHKAL AKYEKICKE QGRQFHVIT 
QNVDGLHKR AGSENVLEL HGSLDKVIC TKCKQIEVN 
TENPICEAL RGRGDPSKR DQDLPIIPL EELPKCSEC 
QALVRPYIV WFGENLDPD VLDRSRQLI ESCDLCLVI 
GTSSVVYPA AMFAPTVVE RGKPVAEFN LNEDAFDDA 
FSFYFSGPC GVTLPKALG IDV
    CDS sequence
    • >TC005187 RA
      atggcaaaa cggccgctc tccgactac acccagttc 
cagagggtt gtgagtcaa gcgcgaagc atcgtcgcc 
ctcacgggg gccggagtg tcggcagag tcggggatt 
cccgtgttc aggggcgcc ggcgggctg tggcgcaca 
caccgcgcc accgacctg gccaccccg accgccttc 
agggccaac ccggcccta gtgtgggaa ttttaccat 
tacagaagg gacgtcgct tttaacagc caaccaaac 
aatgcgcac aaagccttg gccaagtac gaaaagatt 
tgcaaagaa cagggacgc cagttccac gtaatcacg 
caaaacgtt gatggtttg cataagaga gccgggtcg 
gaaaacgtc ctagaactg cacgggagt ctcgacaaa 
gttatctgt actaaatgt aaacaaatt gaagttaac 
accgaaaac ccgatttgt gaagccttg cgtggccgg 
ggggacccg agcaaaagg gaccaagac ctgcctata 
atcccactg gaagaactt ccgaagtgc tccgaatgc 
caggcattg gttcggcct tacattgtt tggttcggc 
gagaattta gatcctgac gttcttgat agatcacga 
caattgatc gaatcgtgt gatttgtgt ctcgttatc 
ggcacttcg tcagttgtg taccctgcg gcgatgttt 
gcacctact gtggtcgag cgggggaaa cccgtagcc 
gagtttaac ttaaacgaa gatgcgttt gatgacgca 
tttagtttt tatttttcc gggccgtgt ggcgttacg 
ttacccaaa gcgcttggg attgatgtg taa
    mRNA sequence
    • >TC005187 RA
      tgctgttaa cgagataaa cctgaagaa atttcatac 
cattccagg taaaatctc caacaaatc ctttttggt 
agcactttc acacactaa ttttgacta tttatttgc 
taacaagcc aaacacctc cacgcgata acaaatcac 
ctttcaacg ataatacgg ctcaaacaa ataaataca 
ctcgaattc caaacacga gttgaagta agtgtccaa 
catatcaac ccgccactc aacaactcc aatctcaac 
cagctcgag atcgccgaa gaactcgac acatgcgca 
cggcgcccc cgtcaaacg tcacacgtc cctaacctc 
gtaaatttg aattaatga tttcacaaa aattccgtc 
atgtcattt cccggcgac tcaacagtg ggctaaaga 
tggcaaaac ggccgctct ccgactaca cccagttcc 
agagggttg tgagtcaag cgcgaagca tcgtcgccc 
tcacggggg ccggagtgt cggcagagt cggggattc 
ccgtgttca ggggcgccg gcgggctgt ggcgcacac 
accgcgcca ccgacctgg ccaccccga ccgccttca 
gggccaacc cggccctag tgtgggaat tttaccatt 
acagaaggg acgtcgctt ttaacagcc aaccaaaca 
atgcgcaca aagccttgg ccaagtacg aaaagattt 
gcaaagaac agggacgcc agttccacg taatcacgc 
aaaacgttg atggtttgc ataagagag ccgggtcgg 
aaaacgtcc tagaactgc acgggagtc tcgacaaag 
ttatctgta ctaaatgta aacaaattg aagttaaca 
ccgaaaacc cgatttgtg aagccttgc gtggccggg 
gggacccga gcaaaaggg accaagacc tgcctataa 
tcccactgg aagaacttc cgaagtgct ccgaatgcc 
aggcattgg ttcggcctt acattgttt ggttcggcg 
agaatttag atcctgacg ttcttgata gatcacgac 
aattgatcg aatcgtgtg atttgtgtc tcgttatcg 
gcacttcgt cagttgtgt accctgcgg cgatgtttg 
cacctactg tggtcgagc gggggaaac ccgtagccg 
agtttaact taaacgaag atgcgtttg atgacgcat 
ttagttttt atttttccg ggccgtgtg gcgttacgt 
tacccaaag cgcttggga ttgatgtgt aaaaaggta 
aaaaaaaaa caggagttt ttattacat atgtaatta 
atttaagaa caataactt agtaataaa tacactgga 
caaatgaga ttatgaaat gtaattttt tgggtattt 
gcgttttta gttcaatgt ttggtcgtt aaatttgta 
caaaggaat aaataaagt gtttaatta ttaagtcgt 
agctattgc actagtcgg tacttagaa aaataatct 
gtatgaaag tctggttgg gcgttattg tcattcgct 
tgctaccct cgtgacagc ccaacgcaa ttaaagcta 
tgacaagaa acataattg aaggca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY