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Gene information
General information
TC009202 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3210572..3213714
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009202
    iBeetle screen iB_08667

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009202 RA
      MFSLQNQNQ AIFTLSQIS AIITPRQNA FLTKKAHKD 
      KSQIMCKES FLEALKNCD FSFTKHLKD TSFLNNFKV 
      SYVSHDTFF ENKHGFSEA DLKSAVPTL IVKNRKHFY 
      VPQNFYQKL IHGLRHENA FTFDIQVRG FKTDRSVKA 
      EMQRNPGLG ARIKNALGL TPSENLDSK LAKDLSLGN 
      EKMKQIFAA EGNSMTEAE KQRIKVAFA EGYLLANSP 
      GARTGKAAR YFKVFQQVL TIVIFLAIV ISLMVSTTG 
      SIFRIQLGN QVEVDPEEI HVTFDDVKG ADEAKQELK 
      DVVEFLKNP DKFSQLGGK LPKGVLLVG PPGTGKTLL 
      ARAVAGEAG VPFFHAAGP EFDEILVGQ GARRVRDLF 
      KSAKEKAPC VIFIDEIDS VGAKRTNSV LHPYANQTI 
      NQLLSEMDG FHQNEGVIV LGATNRRDD LDQALLRPG 
      RFDVEVTVP TPDFTGRKE ILGLYLGKV LAKDVDLEL 
      LARGTTGFT GADLENMVN QAALKAAID GADCVSMKY 
      LESARDKVL MGPERKSRI PDEEANLIT AYHEGGHAI 
      VAFYTRDSH PLHKVTIIP RGPSLGHTA YIPEKERYH 
      VTKSQLLAT MDVMMGGRA AEELIFGTE KITSGASSD 
      LKQATSIAT HMVKDWGMS EKIGLRTMT ESSKPFQGD 
      SLGPSTNEL VDSEIRRIL SESYDRAKH ILKAHAKEH 
      KALAEALMK YETLDAEDI KAIMTEKGP HVEKS
    CDS sequence
    • >TC009202 RA
      atgttttca ctccagaac caaaaccag gcaatcttc 
      actctctcc caaatctcc gcgataata accccccgt 
      cagaatgcc ttcctgacc aaaaaagcc cacaaagac 
      aaatcgcaa atcatgtgc aaggaaagc ttcctggag 
      gcgctcaaa aactgcgac ttctccttc accaagcac 
      ctcaaagac acctcattc ctcaacaac ttcaaagtc 
      tcgtacgtc tcgcacgac actttcttc gaaaacaaa 
      cacggtttc tccgaagcc gacttaaag agcgcagtt 
      ccaaccctc atagtcaaa aatcgcaaa cacttctac 
      gtcccccaa aacttctac cagaagcta atccacggt 
      ttgcggcac gaaaacgcc ttcacgttc gacatccaa 
      gtgcggggc ttcaagaca gaccgcagt gtcaaggcg 
      gaaatgcag cgaaatccg ggcctgggc gcccgcatc 
      aaaaacgcc ctagggctg accccaagc gagaatttg 
      gactcgaaa ttggccaaa gacttgtcg ttgggcaac 
      gaaaagatg aaacagatt ttcgcagcc gaggggaat 
      tcaatgaca gaggcggaa aaacaaagg attaaggtg 
      gcttttgcc gagggctat ctcttggcc aatagcccg 
      ggggcgcgg acgggcaaa gccgcgcgg tattttaag 
      gtgtttcag caggtgttg accattgtg atcttccta 
      gccattgtt attagtttg atggtcagt acaacgggc 
      tcgattttt agaattcaa ttagggaat caagtggag 
      gtggaccct gaggagata catgtgact tttgatgac 
      gtcaagggg gctgatgag gccaaacaa gagcttaaa 
      gacgtagtt gagtttttg aaaaatccc gataaattt 
      tcacaatta gggggcaaa ctaccaaag ggggtgttg 
      ttagtaggg cctccaggt actggtaaa acacttcta 
      gctagggct gtggctggt gaggcgggt gtgcccttt 
      tttcacgct gcagggccg gaatttgat gaaattttg 
      gtggggcaa ggggctagg cgtgtccgg gatttattt 
      aagtctgcc aaagaaaag gccccctgt gtgattttc 
      atcgatgaa atcgattca gttggtgcg aaacggacg 
      aatagtgtt ttacatccg tatgcaaat caaacgatt 
      aatcaactt ttgagcgaa atggacggt tttcatcaa 
      aacgagggg gttattgtt ttgggggca actaatcga 
      agggatgat ttagatcaa gccttgctg agaccggga 
      cggtttgat gttgaggtc acggtccct acgcccgat 
      ttcaccggc cggaaagaa atcctgggc ctctatttg 
      ggcaaagtc cttgccaag gacgtggat ttggaactc 
      ttggcccgg ggaaccacc ggttttacg ggcgcagac 
      ctcgaaaac atggtcaat caagctgct ctcaaggct 
      gccatcgat ggggccgat tgcgttagc atgaaatac 
      ctcgaatcg gcgcgcgat aaagtctta atgggcccc 
      gaacgcaaa tcacgaatt cccgacgaa gaagccaac 
      cttataaca gcgtaccat gaagggggc cacgcgatt 
      gtggcgttc tacacgcgt gacagccat ccgttgcat 
      aaagttacg ataataccg agggggccc agtttgggg 
      cacacggcc tacattccg gagaaggag cgctatcat 
      gtgaccaaa tcgcaactt ttggcaacg atggatgtc 
      atgatgggg ggcagagcc gccgaagag ctgattttc 
      gggacggaa aaaatcacc tcaggggcg agctcggat 
      ttgaagcaa gcgacgtcc atagccacc catatggtg 
      aaggactgg ggcatgtcg gagaaaatc ggcctgagg 
      acgatgact gaaagctcg aagccgttc caaggcgac 
      agtttgggg ccgtcgact aacgaactc gttgatagc 
      gaaattagg agaattctg tcagaaagt tacgacaga 
      gccaaacat attctcaaa gcgcatgcc aaggaacat 
      aaggcctta gccgaggct ttgatgaag tacgagacg 
      ttagatgcg gaagatatt aaagccata atgacagaa 
      aaagggcca catgttgaa aaatcttaa
    mRNA sequence
    • >TC009202 RA
      tgagtttgg agggcttga gggaggtat aattgactg 
      tggttgatt tttggttgg gtaattatg attgatttg 
      tgttggggg agcgggcca gaagtgtgt tggcgggcc 
      tggctggtg cctagtgga atcatctgt taagtgtca 
      gaaaggtta tatgtggta tttttataa aaattcgat 
      caaattcgt cgttgtttt ttatgtaac gtaactaat 
      cacttcatg taagtcaca atgttttca ctccagaac 
      caaaaccag gcaatcttc actctctcc caaatctcc 
      gcgataata accccccgt cagaatgcc ttcctgacc 
      aaaaaagcc cacaaagac aaatcgcaa atcatgtgc 
      aaggaaagc ttcctggag gcgctcaaa aactgcgac 
      ttctccttc accaagcac ctcaaagac acctcattc 
      ctcaacaac ttcaaagtc tcgtacgtc tcgcacgac 
      actttcttc gaaaacaaa cacggtttc tccgaagcc 
      gacttaaag agcgcagtt ccaaccctc atagtcaaa 
      aatcgcaaa cacttctac gtcccccaa aacttctac 
      cagaagcta atccacggt ttgcggcac gaaaacgcc 
      ttcacgttc gacatccaa gtgcggggc ttcaagaca 
      gaccgcagt gtcaaggcg gaaatgcag cgaaatccg 
      ggcctgggc gcccgcatc aaaaacgcc ctagggctg 
      accccaagc gagaatttg gactcgaaa ttggccaaa 
      gacttgtcg ttgggcaac gaaaagatg aaacagatt 
      ttcgcagcc gaggggaat tcaatgaca gaggcggaa 
      aaacaaagg attaaggtg gcttttgcc gagggctat 
      ctcttggcc aatagcccg ggggcgcgg acgggcaaa 
      gccgcgcgg tattttaag gtgtttcag caggtgttg 
      accattgtg atcttccta gccattgtt attagtttg 
      atggtcagt acaacgggc tcgattttt agaattcaa 
      ttagggaat caagtggag gtggaccct gaggagata 
      catgtgact tttgatgac gtcaagggg gctgatgag 
      gccaaacaa gagcttaaa gacgtagtt gagtttttg 
      aaaaatccc gataaattt tcacaatta gggggcaaa 
      ctaccaaag ggggtgttg ttagtaggg cctccaggt 
      actggtaaa acacttcta gctagggct gtggctggt 
      gaggcgggt gtgcccttt tttcacgct gcagggccg 
      gaatttgat gaaattttg gtggggcaa ggggctagg 
      cgtgtccgg gatttattt aagtctgcc aaagaaaag 
      gccccctgt gtgattttc atcgatgaa atcgattca 
      gttggtgcg aaacggacg aatagtgtt ttacatccg 
      tatgcaaat caaacgatt aatcaactt ttgagcgaa 
      atggacggt tttcatcaa aacgagggg gttattgtt 
      ttgggggca actaatcga agggatgat ttagatcaa 
      gccttgctg agaccggga cggtttgat gttgaggtc 
      acggtccct acgcccgat ttcaccggc cggaaagaa 
      atcctgggc ctctatttg ggcaaagtc cttgccaag 
      gacgtggat ttggaactc ttggcccgg ggaaccacc 
      ggttttacg ggcgcagac ctcgaaaac atggtcaat 
      caagctgct ctcaaggct gccatcgat ggggccgat 
      tgcgttagc atgaaatac ctcgaatcg gcgcgcgat 
      aaagtctta atgggcccc gaacgcaaa tcacgaatt 
      cccgacgaa gaagccaac cttataaca gcgtaccat 
      gaagggggc cacgcgatt gtggcgttc tacacgcgt 
      gacagccat ccgttgcat aaagttacg ataataccg 
      agggggccc agtttgggg cacacggcc tacattccg 
      gagaaggag cgctatcat gtgaccaaa tcgcaactt 
      ttggcaacg atggatgtc atgatgggg ggcagagcc 
      gccgaagag ctgattttc gggacggaa aaaatcacc 
      tcaggggcg agctcggat ttgaagcaa gcgacgtcc 
      atagccacc catatggtg aaggactgg ggcatgtcg 
      gagaaaatc ggcctgagg acgatgact gaaagctcg 
      aagccgttc caaggcgac agtttgggg ccgtcgact 
      aacgaactc gttgatagc gaaattagg agaattctg 
      tcagaaagt tacgacaga gccaaacat attctcaaa 
      gcgcatgcc aaggaacat aaggcctta gccgaggct 
      ttgatgaag tacgagacg ttagatgcg gaagatatt 
      aaagccata atgacagaa aaagggcca catgttgaa 
      aaatcttaa gtggatttg ctacagact tttttatga 
      cagtacgct gtgttaaaa tcgggtttc ttcgcttta 
      gttagccac tgttttgta catatttac ataataaag 
      cgtaagcag gccttcggt tacttaatt tcatgagta 
      agtatgtga agttgctgt aatataaac aataaaatt 
      ttattatcg aa
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