Status
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Gene information
General information
TC009201 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3218000..3223664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009201
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08666

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    abdominal odoriferous glands content split - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009201 RA
      MSSEESTES RQNNELEAL QAIYGDDLR DLRGKSAWN 
      AWTPLNLSI SLNPQQGSS GLHEVYVKL DLHVTCPET 
      YPNTVPVIK LENSKGLSD NSLIQLQKI LEEKADKLR 
      GEEMIFQLA QHVQEFLHL HNKPASKSF YDEMLQRQK 
      EKEEKDLQA KLMEEDRER QFVQQEVQK RQEILKLES 
      RMRRYNSEN SDDSDQRRR SISYNSNEN SDESLCSHK 
      KTVIVEFTN REIKRGQCL KHCSYNHVV FIGMDVKTG 
      EIVLLSEWI ITNRKYLSS LEQEFNYLA KLKHQNLAH 
      YLSFKYEGH ADGAVVHIL KEYVSGPNC SFFMNENLS 
      VSIQFIKYV AKGVLTALD YLHRNNVVH KDIRESCVY 
      ISEKGIVAL TNYSLHRRL TDISSQTNI TSNYSKKTD 
      IYKFGVFLI SLLQGASLT EDDDVDIPP CVPSDLYDF 
      LSRCLEKQE KERFTAVQL LNHSFLRQN SLLSSPGHK 
      IDHNIPDGN ISPDLATDE LNPLQHQSG QSRIEKEFE 
      IIDNLGKGA YGDVIKARN NLDGRYYAI KRIKLNPRN 
      RQLNKKIVR EVKLLSRLN HENIVRYYN SWIERTVIK 
      GDDSSDMES TTSGIDDKC PRIVRPDEF TLNDNIEEL 
      APSIKNVEV SVTFDSISQ PAYDSSSDE SSCDDENSL 
      DIYEESDSD GIEFEKDSH APSPSDPPS TKSPQLLET 
      GRQIEFMYI QMEFCEKST LRNAIDNSV YLDEDRVWR 
      LFREIVEGL AHIHQQGMI HRDLKPVNI FLDSNDHVK 
      IGDFGLATN MTPKLNEHD LNRTFPERY DMDESKTGL 
      VGTALYVAP ELNVASKAN YNQKVDIYS LGIIFFEMC 
      YKPLLTNME RIKILTSLR KKEIVLPED FAPGNPKQK 
      FLVTWLLNH DVAKRPTSV ELLQSEHIP PPVLEECEL 
      RELVRHTLS NPQLKGYKY LIASCFDQT LTPAQNITY 
      DKDSAVPTL NKPQIYDFV KETIVKIFK QHGAQNLTT 
      PLLMPKSHY YEGVESCVK LMTHFGGIV SLPHDLRVP 
      FARYVAWNR ITLLRRYSV ERVYREKRV FGFLPRELY 
      ECAFDIVTP SPGNHTSDA EVLYVIYEI INELPGVKN 
      KHVTIRLNH SLLMKAILL HCGVKNRHE EILQKFSDV 
      KDGKVPKSD LQDYLIQFG LSDNAISTL TNIFSSEFE 
      VSKAANHFQ SITRKKSSE ASHLAKQAL QELKSIAYN 
      AEAFGVTFE MVVAPGAVY NIQQYSGMI CQFVCELKK 
      KHKHGNKEV LAAGGRYDA MITSYRKIM ENANMLARD 
      IKQSAVGVS LSLDKLAQA VMREQEDLP SLEVLDVAV 
      CSVGTKPLL REKTKILRN LWSAGIRCA MIEATNIEE 
      IQEQCGELK VPHVVMLKD SEQGTVRVR SWERDRFQE 
      KTVKTPELV ETMQRIIKC WKEGAQEVL PITGTVFRS 
      DSKSSYDKN EHEATVAVL FVTEDKLSA NARRRYDIQ 
      IRSHLDVLV KKLSGFVTV LGLSVESNV IRTIASYLE 
      WETDQQFHK SVQAIIEKH QRHKKYLTD VCDEIYEQK 
      SKKTKPAVI LFSLLDSFY RLLI
    CDS sequence
    • >TC009201 RA
      atgtcctcg gaagagtca acagagtcc cggcaaaac 
      aacgagttg gaagcgctc caggccatt tacggcgac 
      gacttgagg gacctgcga ggaaaatcg gcctggaac 
      gcctggacc cctttaaat ttatcaatc tctctgaac 
      ccccaacag ggcagttcg ggcctccac gaagtctac 
      gtcaagctt gatttgcac gtcacgtgc cccgaaacg 
      taccccaac actgttcct gtgattaaa ttagagaac 
      agtaaaggc ctgtccgac aattccctc atccagctc 
      cagaaaatc ctagaggaa aaggcggac aagttaagg 
      ggcgaggag atgattttt cagttggcg cagcacgtc 
      caggagttc ctccacttg cacaacaaa ccggcgtcg 
      aagagcttc tacgacgag atgctccag agacagaag 
      gaaaaggag gagaaggat ttacaagcc aaactaatg 
      gaggaagat agagaaagg caattcgta caacaagaa 
      gtacaaaag cggcaagaa attttgaaa ttggagtcg 
      cgcatgcgc cgttacaat tccgaaaac tccgacgac 
      agtgatcaa aggcggcgc tcgatctcg tacaactcg 
      aatgaaaac agcgatgag tctctctgt agccacaaa 
      aagaccgta attgtggaa ttcaccaat cgtgagatc 
      aagcgaggc cagtgctta aaacattgc agctacaat 
      cacgtggtt tttatcgga atggacgtc aagaccggc 
      gaaattgtc cttttgagc gagtggatc atcaccaat 
      cgtaaatat ctgtcgagt cttgagcaa gaatttaat 
      tatttggcc aaactgaag caccaaaac ctcgcccac 
      tatttgagt tttaagtac gaagggcat gcagatggc 
      gctgttgtt catattttg aaagagtat gtcagtggg 
      cccaactgt tcgtttttc atgaatgag aatctcagt 
      gtgagcatc cagtttatt aaatatgtg gcgaagggt 
      gtgctcact gctctcgat tatttgcat aggaataac 
      gtcgtgcat aaagacata agagagagt tgtgtgtac 
      atcagtgag aaaggcatt gttgcgctg actaattat 
      agtttgcat cgcagactg actgatatc tcctcacaa 
      acgaacatt acctccaat tatagcaaa aagactgat 
      atttataaa tttggcgtg ttcttaatt tcactattg 
      caaggcgcg agcttaaca gaggacgat gatgtcgat 
      attccgcct tgcgttcca tcagatctc tatgatttt 
      ttatcaaga tgtttggaa aagcaggaa aaagagcgt 
      tttaccgca gtccaatta ttaaatcat tcgtttctt 
      cgccagaac agtttatta tcgtcacct ggtcacaaa 
      atcgaccat aatattcca gatgggaac atttcgcct 
      gatttggca accgatgaa cttaatcca ttgcagcat 
      caaagtggc cagtctcgc attgagaaa gagtttgaa 
      attatcgat aatttgggc aagggggcg tatggggac 
      gttattaag gctaggaat aatttagat gggagatat 
      tacgcaatc aaacgcatt aaattaaac cccaggaac 
      cgacaatta aataaaaaa attgttcgt gaagttaag 
      ctcttatcg aggctaaat catgaaaat atagtgcgt 
      tattataat tcctggatt gaacggacg gtgataaag 
      ggtgacgac agcagtgat atggaaagt acaacgtcg 
      gggattgac gataagtgt ccaaggata gtccgtcca 
      gatgagttc accctcaat gacaatatt gaagagctg 
      gcgccctct ataaaaaac gtggaagtt tccgtaacg 
      tttgacagc atatcacag cctgcttac gactcgtca 
      tcggatgaa tcaagctgt gatgacgag aacagcctg 
      gacatttac gaggaatca gattcggac ggaatcgag 
      ttcgaaaaa gactcacat gcccccagc cccagcgac 
      cccccgtcc accaaaagc ccccaactg ctcgaaacg 
      gggcgccaa atcgaattc atgtacatc cagatggag 
      ttttgcgaa aagagcacc ctacgtaac gccatagat 
      aacagtgtg tacctagac gaggaccga gtgtggcgc 
      ctcttccgc gaaatagtt gaaggctta gcccacatc 
      caccagcaa ggcatgatc catcgtgac ctcaaaccc 
      gtcaatatt ttcctcgat tcgaacgat cacgtgaaa 
      atcggcgat ttcggattg gccacgaac atgacaccg 
      aagctaaac gagcacgat ttgaaccga acctttccg 
      gaacggtac gacatggac gagagcaag acgggattg 
      gtgggcaca gcactctac gtggcgccg gaattaaac 
      gtcgcctcc aaggcgaat tacaaccaa aaggttgat 
      atttacagt ttgggcatt atttttttc gaaatgtgt 
      tacaagccc ttgttgacc aacatggaa aggatcaag 
      attttgacg agtttgcgg aagaaagag atcgtgtta 
      cctgaggat ttcgcaccg gggaatccc aaacagaag 
      tttctggtc acgtggttg ttgaatcat gacgttgcg 
      aaacggcca acttcggtc gagttgcta caatcggaa 
      catataccg ccccctgtt ttggaagaa tgtgaatta 
      agagaactg gttaggcac actttaagt aacccacaa 
      ttgaaaggt tacaagtac ttgatagca tcatgtttt 
      gatcagacg ttaacaccg gcacaaaac ataacgtat 
      gataaggat tcagcagtg ccaacttta aataaacca 
      caaatttat gactttgtc aaagaaaca atagtcaag 
      attttcaaa caacatggg gcccagaat ttgacaacg 
      cctctttta atgcccaaa agtcactac tacgagggc 
      gtggagtct tgtgtcaaa cttatgaca cactttgga 
      ggtattgtt tcgctcccg catgacttg agggttccg 
      tttgcccgt tatgtggcc tggaaccga atcaccctt 
      ttacgtcgc tactcagtc gagcgtgtt tatcgcgaa 
      aaacgcgtt ttcgggttt ttaccgcgt gaattgtac 
      gagtgcgcc tttgatatc gtcaccccg agcccgggg 
      aatcacaca tcagacgcc gaagttttg tacgtcatt 
      tacgaaatc atcaacgaa cttccgggg gtgaagaac 
      aaacacgtc acgatccga ctcaaccat tcgctgctg 
      atgaaggcg attctgttg cattgtggc gtcaagaac 
      cgacacgag gagatttta cagaaattc tccgatgtt 
      aaagacggt aaagtaccg aaaagcgac ctgcaagac 
      tacctgatc cagttcggc ctatccgac aatgcaatt 
      tcgacccta actaacatt tttagttcg gagtttgag 
      gtcagcaag gccgcaaac cacttccag tcaatcact 
      cggaaaaaa tcgagcgag gcgagccat ttggccaag 
      caggcgttg caagaattg aaaagtata gcatacaat 
      gcggaggcg ttcggagtc acgtttgaa atggtggtg 
      gcacccggt gccgtgtac aacatccaa caatattcc 
      ggaatgatt tgtcagttt gtttgcgag ttaaaaaag 
      aagcacaaa cacggtaac aaggaggtt ttggccgcg 
      ggagggcgc tacgacgct atgataaca tcgtaccgt 
      aaaataatg gaaaatgcg aacatgtta gccagggat 
      attaaacaa tcagctgtt ggtgtttcc ctctctttg 
      gacaagttg gcccaggca gtgatgagg gaacaggag 
      gatttgccc tcacttgag gtgctggac gttgcggtt 
      tgttcagtt ggcaccaaa ccgctcctc agggagaaa 
      actaagatt ttgcgcaat ttgtggtcg gccgggatt 
      cgctgtgcc atgattgag gcaacaaat attgaagag 
      attcaggaa cagtgtggc gagttaaaa gtgcctcac 
      gttgttatg ttgaaagac agcgagcaa gggactgtg 
      cgtgtgcgc agttgggaa cgcgatcgc tttcaggag 
      aaaacggtt aaaacgcca gaactggtc gaaaccatg 
      caacgaatc atcaagtgc tggaaggag ggggcgcag 
      gaggtgctc ccaatcact ggaactgtc ttcaggtca 
      gatagtaaa agcagttat gacaaaaat gagcacgag 
      gccaccgtt gccgtcttg tttgttacg gaggataag 
      ctgtcagcc aatgcgaga cggcgctac gatattcaa 
      atccgatct catttagac gttttggtg aagaaattg 
      agcggtttt gtgactgtt ttggggctc agtgtcgaa 
      tcaaatgtc attcggacc atagcttcg tacctggag 
      tgggaaacc gaccaacag tttcacaaa tcggtccaa 
      gccataatc gagaagcac cagcgccac aaaaaatac 
      ctaactgac gtctgtgat gaaatatac gaacagaaa 
      agcaaaaag acgaaacct gcggtgatc ctgttcagt 
      ctgctcgac tcgttttat aggttgtta atttag
    mRNA sequence
    • >TC009201 RA
      caactccag tcggaatca tgtcctcgg aagagtcaa 
      cagagtccc ggcaaaaca acgagttgg aagcgctcc 
      aggccattt acggcgacg acttgaggg acctgcgag 
      gaaaatcgg cctggaacg cctggaccc ctttaaatt 
      tatcaatct ctctgaacc cccaacagg gcagttcgg 
      gcctccacg aagtctacg tcaagcttg atttgcacg 
      tcacgtgcc ccgaaacgt accccaaca ctgttcctg 
      tgattaaat tagagaaca gtaaaggcc tgtccgaca 
      attccctca tccagctcc agaaaatcc tagaggaaa 
      aggcggaca agttaaggg gcgaggaga tgatttttc 
      agttggcgc agcacgtcc aggagttcc tccacttgc 
      acaacaaac cggcgtcga agagcttct acgacgaga 
      tgctccaga gacagaagg aaaaggagg agaaggatt 
      tacaagcca aactaatgg aggaagata gagaaaggc 
      aattcgtac aacaagaag tacaaaagc ggcaagaaa 
      ttttgaaat tggagtcgc gcatgcgcc gttacaatt 
      ccgaaaact ccgacgaca gtgatcaaa ggcggcgct 
      cgatctcgt acaactcga atgaaaaca gcgatgagt 
      ctctctgta gccacaaaa agaccgtaa ttgtggaat 
      tcaccaatc gtgagatca agcgaggcc agtgcttaa 
      aacattgca gctacaatc acgtggttt ttatcggaa 
      tggacgtca agaccggcg aaattgtcc ttttgagcg 
      agtggatca tcaccaatc gtaaatatc tgtcgagtc 
      ttgagcaag aatttaatt atttggcca aactgaagc 
      accaaaacc tcgcccact atttgagtt ttaagtacg 
      aagggcatg cagatggcg ctgttgttc atattttga 
      aagagtatg tcagtgggc ccaactgtt cgtttttca 
      tgaatgaga atctcagtg tgagcatcc agtttatta 
      aatatgtgg cgaagggtg tgctcactg ctctcgatt 
      atttgcata ggaataacg tcgtgcata aagacataa 
      gagagagtt gtgtgtaca tcagtgaga aaggcattg 
      ttgcgctga ctaattata gtttgcatc gcagactga 
      ctgatatct cctcacaaa cgaacatta cctccaatt 
      atagcaaaa agactgata tttataaat ttggcgtgt 
      tcttaattt cactattgc aaggcgcga gcttaacag 
      aggacgatg atgtcgata ttccgcctt gcgttccat 
      cagatctct atgattttt tatcaagat gtttggaaa 
      agcaggaaa aagagcgtt ttaccgcag tccaattat 
      taaatcatt cgtttcttc gccagaaca gtttattat 
      cgtcacctg gtcacaaaa tcgaccata atattccag 
      atgggaaca tttcgcctg atttggcaa ccgatgaac 
      ttaatccat tgcagcatc aaagtggcc agtctcgca 
      ttgagaaag agtttgaaa ttatcgata atttgggca 
      agggggcgt atggggacg ttattaagg ctaggaata 
      atttagatg ggagatatt acgcaatca aacgcatta 
      aattaaacc ccaggaacc gacaattaa ataaaaaaa 
      ttgttcgtg aagttaagc tcttatcga ggctaaatc 
      atgaaaata tagtgcgtt attataatt cctggattg 
      aacggacgg tgataaagg gtgacgaca gcagtgata 
      tggaaagta caacgtcgg ggattgacg ataagtgtc 
      caaggatag tccgtccag atgagttca ccctcaatg 
      acaatattg aagagctgg cgccctcta taaaaaacg 
      tggaagttt ccgtaacgt ttgacagca tatcacagc 
      ctgcttacg actcgtcat cggatgaat caagctgtg 
      atgacgaga acagcctgg acatttacg aggaatcag 
      attcggacg gaatcgagt tcgaaaaag actcacatg 
      cccccagcc ccagcgacc ccccgtcca ccaaaagcc 
      cccaactgc tcgaaacgg ggcgccaaa tcgaattca 
      tgtacatcc agatggagt tttgcgaaa agagcaccc 
      tacgtaacg ccatagata acagtgtgt acctagacg 
      aggaccgag tgtggcgcc tcttccgcg aaatagttg 
      aaggcttag cccacatcc accagcaag gcatgatcc 
      atcgtgacc tcaaacccg tcaatattt tcctcgatt 
      cgaacgatc acgtgaaaa tcggcgatt tcggattgg 
      ccacgaaca tgacaccga agctaaacg agcacgatt 
      tgaaccgaa cctttccgg aacggtacg acatggacg 
      agagcaaga cgggattgg tgggcacag cactctacg 
      tggcgccgg aattaaacg tcgcctcca aggcgaatt 
      acaaccaaa aggttgata tttacagtt tgggcatta 
      tttttttcg aaatgtgtt acaagccct tgttgacca 
      acatggaaa ggatcaaga ttttgacga gtttgcgga 
      agaaagaga tcgtgttac ctgaggatt tcgcaccgg 
      ggaatccca aacagaagt ttctggtca cgtggttgt 
      tgaatcatg acgttgcga aacggccaa cttcggtcg 
      agttgctac aatcggaac atataccgc cccctgttt 
      tggaagaat gtgaattaa gagaactgg ttaggcaca 
      ctttaagta acccacaat tgaaaggtt acaagtact 
      tgatagcat catgttttg atcagacgt taacaccgg 
      cacaaaaca taacgtatg ataaggatt cagcagtgc 
      caactttaa ataaaccac aaatttatg actttgtca 
      aagaaacaa tagtcaaga ttttcaaac aacatgggg 
      cccagaatt tgacaacgc ctcttttaa tgcccaaaa 
      gtcactact acgagggcg tggagtctt gtgtcaaac 
      ttatgacac actttggag gtattgttt cgctcccgc 
      atgacttga gggttccgt ttgcccgtt atgtggcct 
      ggaaccgaa tcacccttt tacgtcgct actcagtcg 
      agcgtgttt atcgcgaaa aacgcgttt tcgggtttt 
      taccgcgtg aattgtacg agtgcgcct ttgatatcg 
      tcaccccga gcccgggga atcacacat cagacgccg 
      aagttttgt acgtcattt acgaaatca tcaacgaac 
      ttccggggg tgaagaaca aacacgtca cgatccgac 
      tcaaccatt cgctgctga tgaaggcga ttctgttgc 
      attgtggcg tcaagaacc gacacgagg agattttac 
      agaaattct ccgatgtta aagacggta aagtaccga 
      aaagcgacc tgcaagact acctgatcc agttcggcc 
      tatccgaca atgcaattt cgaccctaa ctaacattt 
      ttagttcgg agtttgagg tcagcaagg ccgcaaacc 
      acttccagt caatcactc ggaaaaaat cgagcgagg 
      cgagccatt tggccaagc aggcgttgc aagaattga 
      aaagtatag catacaatg cggaggcgt tcggagtca 
      cgtttgaaa tggtggtgg cacccggtg ccgtgtaca 
      acatccaac aatattccg gaatgattt gtcagtttg 
      tttgcgagt taaaaaaga agcacaaac acggtaaca 
      aggaggttt tggccgcgg gagggcgct acgacgcta 
      tgataacat cgtaccgta aaataatgg aaaatgcga 
      acatgttag ccagggata ttaaacaat cagctgttg 
      gtgtttccc tctctttgg acaagttgg cccaggcag 
      tgatgaggg aacaggagg atttgccct cacttgagg 
      tgctggacg ttgcggttt gttcagttg gcaccaaac 
      cgctcctca gggagaaaa ctaagattt tgcgcaatt 
      tgtggtcgg ccgggattc gctgtgcca tgattgagg 
      caacaaata ttgaagaga ttcaggaac agtgtggcg 
      agttaaaag tgcctcacg ttgttatgt tgaaagaca 
      gcgagcaag ggactgtgc gtgtgcgca gttgggaac 
      gcgatcgct ttcaggaga aaacggtta aaacgccag 
      aactggtcg aaaccatgc aacgaatca tcaagtgct 
      ggaaggagg gggcgcagg aggtgctcc caatcactg 
      gaactgtct tcaggtcag atagtaaaa gcagttatg 
      acaaaaatg agcacgagg ccaccgttg ccgtcttgt 
      ttgttacgg aggataagc tgtcagcca atgcgagac 
      ggcgctacg atattcaaa tccgatctc atttagacg 
      ttttggtga agaaattga gcggttttg tgactgttt 
      tggggctca gtgtcgaat caaatgtca ttcggacca 
      tagcttcgt acctggagt gggaaaccg accaacagt 
      ttcacaaat cggtccaag ccataatcg agaagcacc 
      agcgccaca aaaaatacc taactgacg tctgtgatg 
      aaatatacg aacagaaaa gcaaaaaga cgaaacctg 
      cggtgatcc tgttcagtc tgctcgact cgttttata 
      ggttgttaa tttaggtaa attattttt ttacatgta 
      ttgtttgat aagattaca atcttagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY