| General information | |||
| TC009201 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3218000..3223664 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009201 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08666 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
abdominal odoriferous glands content split - Number of animals showing the phenotype: 3 (13 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009201 RA
MSSEESTES RQNNELEAL QAIYGDDLR DLRGKSAWN AWTPLNLSI SLNPQQGSS GLHEVYVKL DLHVTCPET YPNTVPVIK LENSKGLSD NSLIQLQKI LEEKADKLR GEEMIFQLA QHVQEFLHL HNKPASKSF YDEMLQRQK EKEEKDLQA KLMEEDRER QFVQQEVQK RQEILKLES RMRRYNSEN SDDSDQRRR SISYNSNEN SDESLCSHK KTVIVEFTN REIKRGQCL KHCSYNHVV FIGMDVKTG EIVLLSEWI ITNRKYLSS LEQEFNYLA KLKHQNLAH YLSFKYEGH ADGAVVHIL KEYVSGPNC SFFMNENLS VSIQFIKYV AKGVLTALD YLHRNNVVH KDIRESCVY ISEKGIVAL TNYSLHRRL TDISSQTNI TSNYSKKTD IYKFGVFLI SLLQGASLT EDDDVDIPP CVPSDLYDF LSRCLEKQE KERFTAVQL LNHSFLRQN SLLSSPGHK IDHNIPDGN ISPDLATDE LNPLQHQSG QSRIEKEFE IIDNLGKGA YGDVIKARN NLDGRYYAI KRIKLNPRN RQLNKKIVR EVKLLSRLN HENIVRYYN SWIERTVIK GDDSSDMES TTSGIDDKC PRIVRPDEF TLNDNIEEL APSIKNVEV SVTFDSISQ PAYDSSSDE SSCDDENSL DIYEESDSD GIEFEKDSH APSPSDPPS TKSPQLLET GRQIEFMYI QMEFCEKST LRNAIDNSV YLDEDRVWR LFREIVEGL AHIHQQGMI HRDLKPVNI FLDSNDHVK IGDFGLATN MTPKLNEHD LNRTFPERY DMDESKTGL VGTALYVAP ELNVASKAN YNQKVDIYS LGIIFFEMC YKPLLTNME RIKILTSLR KKEIVLPED FAPGNPKQK FLVTWLLNH DVAKRPTSV ELLQSEHIP PPVLEECEL RELVRHTLS NPQLKGYKY LIASCFDQT LTPAQNITY DKDSAVPTL NKPQIYDFV KETIVKIFK QHGAQNLTT PLLMPKSHY YEGVESCVK LMTHFGGIV SLPHDLRVP FARYVAWNR ITLLRRYSV ERVYREKRV FGFLPRELY ECAFDIVTP SPGNHTSDA EVLYVIYEI INELPGVKN KHVTIRLNH SLLMKAILL HCGVKNRHE EILQKFSDV KDGKVPKSD LQDYLIQFG LSDNAISTL TNIFSSEFE VSKAANHFQ SITRKKSSE ASHLAKQAL QELKSIAYN AEAFGVTFE MVVAPGAVY NIQQYSGMI CQFVCELKK KHKHGNKEV LAAGGRYDA MITSYRKIM ENANMLARD IKQSAVGVS LSLDKLAQA VMREQEDLP SLEVLDVAV CSVGTKPLL REKTKILRN LWSAGIRCA MIEATNIEE IQEQCGELK VPHVVMLKD SEQGTVRVR SWERDRFQE KTVKTPELV ETMQRIIKC WKEGAQEVL PITGTVFRS DSKSSYDKN EHEATVAVL FVTEDKLSA NARRRYDIQ IRSHLDVLV KKLSGFVTV LGLSVESNV IRTIASYLE WETDQQFHK SVQAIIEKH QRHKKYLTD VCDEIYEQK SKKTKPAVI LFSLLDSFY RLLI
- >TC009201 RA
atgtcctcg gaagagtca acagagtcc cggcaaaac aacgagttg gaagcgctc caggccatt tacggcgac gacttgagg gacctgcga ggaaaatcg gcctggaac gcctggacc cctttaaat ttatcaatc tctctgaac ccccaacag ggcagttcg ggcctccac gaagtctac gtcaagctt gatttgcac gtcacgtgc cccgaaacg taccccaac actgttcct gtgattaaa ttagagaac agtaaaggc ctgtccgac aattccctc atccagctc cagaaaatc ctagaggaa aaggcggac aagttaagg ggcgaggag atgattttt cagttggcg cagcacgtc caggagttc ctccacttg cacaacaaa ccggcgtcg aagagcttc tacgacgag atgctccag agacagaag gaaaaggag gagaaggat ttacaagcc aaactaatg gaggaagat agagaaagg caattcgta caacaagaa gtacaaaag cggcaagaa attttgaaa ttggagtcg cgcatgcgc cgttacaat tccgaaaac tccgacgac agtgatcaa aggcggcgc tcgatctcg tacaactcg aatgaaaac agcgatgag tctctctgt agccacaaa aagaccgta attgtggaa ttcaccaat cgtgagatc aagcgaggc cagtgctta aaacattgc agctacaat cacgtggtt tttatcgga atggacgtc aagaccggc gaaattgtc cttttgagc gagtggatc atcaccaat cgtaaatat ctgtcgagt cttgagcaa gaatttaat tatttggcc aaactgaag caccaaaac ctcgcccac tatttgagt tttaagtac gaagggcat gcagatggc gctgttgtt catattttg aaagagtat gtcagtggg cccaactgt tcgtttttc atgaatgag aatctcagt gtgagcatc cagtttatt aaatatgtg gcgaagggt gtgctcact gctctcgat tatttgcat aggaataac gtcgtgcat aaagacata agagagagt tgtgtgtac atcagtgag aaaggcatt gttgcgctg actaattat agtttgcat cgcagactg actgatatc tcctcacaa acgaacatt acctccaat tatagcaaa aagactgat atttataaa tttggcgtg ttcttaatt tcactattg caaggcgcg agcttaaca gaggacgat gatgtcgat attccgcct tgcgttcca tcagatctc tatgatttt ttatcaaga tgtttggaa aagcaggaa aaagagcgt tttaccgca gtccaatta ttaaatcat tcgtttctt cgccagaac agtttatta tcgtcacct ggtcacaaa atcgaccat aatattcca gatgggaac atttcgcct gatttggca accgatgaa cttaatcca ttgcagcat caaagtggc cagtctcgc attgagaaa gagtttgaa attatcgat aatttgggc aagggggcg tatggggac gttattaag gctaggaat aatttagat gggagatat tacgcaatc aaacgcatt aaattaaac cccaggaac cgacaatta aataaaaaa attgttcgt gaagttaag ctcttatcg aggctaaat catgaaaat atagtgcgt tattataat tcctggatt gaacggacg gtgataaag ggtgacgac agcagtgat atggaaagt acaacgtcg gggattgac gataagtgt ccaaggata gtccgtcca gatgagttc accctcaat gacaatatt gaagagctg gcgccctct ataaaaaac gtggaagtt tccgtaacg tttgacagc atatcacag cctgcttac gactcgtca tcggatgaa tcaagctgt gatgacgag aacagcctg gacatttac gaggaatca gattcggac ggaatcgag ttcgaaaaa gactcacat gcccccagc cccagcgac cccccgtcc accaaaagc ccccaactg ctcgaaacg gggcgccaa atcgaattc atgtacatc cagatggag ttttgcgaa aagagcacc ctacgtaac gccatagat aacagtgtg tacctagac gaggaccga gtgtggcgc ctcttccgc gaaatagtt gaaggctta gcccacatc caccagcaa ggcatgatc catcgtgac ctcaaaccc gtcaatatt ttcctcgat tcgaacgat cacgtgaaa atcggcgat ttcggattg gccacgaac atgacaccg aagctaaac gagcacgat ttgaaccga acctttccg gaacggtac gacatggac gagagcaag acgggattg gtgggcaca gcactctac gtggcgccg gaattaaac gtcgcctcc aaggcgaat tacaaccaa aaggttgat atttacagt ttgggcatt atttttttc gaaatgtgt tacaagccc ttgttgacc aacatggaa aggatcaag attttgacg agtttgcgg aagaaagag atcgtgtta cctgaggat ttcgcaccg gggaatccc aaacagaag tttctggtc acgtggttg ttgaatcat gacgttgcg aaacggcca acttcggtc gagttgcta caatcggaa catataccg ccccctgtt ttggaagaa tgtgaatta agagaactg gttaggcac actttaagt aacccacaa ttgaaaggt tacaagtac ttgatagca tcatgtttt gatcagacg ttaacaccg gcacaaaac ataacgtat gataaggat tcagcagtg ccaacttta aataaacca caaatttat gactttgtc aaagaaaca atagtcaag attttcaaa caacatggg gcccagaat ttgacaacg cctctttta atgcccaaa agtcactac tacgagggc gtggagtct tgtgtcaaa cttatgaca cactttgga ggtattgtt tcgctcccg catgacttg agggttccg tttgcccgt tatgtggcc tggaaccga atcaccctt ttacgtcgc tactcagtc gagcgtgtt tatcgcgaa aaacgcgtt ttcgggttt ttaccgcgt gaattgtac gagtgcgcc tttgatatc gtcaccccg agcccgggg aatcacaca tcagacgcc gaagttttg tacgtcatt tacgaaatc atcaacgaa cttccgggg gtgaagaac aaacacgtc acgatccga ctcaaccat tcgctgctg atgaaggcg attctgttg cattgtggc gtcaagaac cgacacgag gagatttta cagaaattc tccgatgtt aaagacggt aaagtaccg aaaagcgac ctgcaagac tacctgatc cagttcggc ctatccgac aatgcaatt tcgacccta actaacatt tttagttcg gagtttgag gtcagcaag gccgcaaac cacttccag tcaatcact cggaaaaaa tcgagcgag gcgagccat ttggccaag caggcgttg caagaattg aaaagtata gcatacaat gcggaggcg ttcggagtc acgtttgaa atggtggtg gcacccggt gccgtgtac aacatccaa caatattcc ggaatgatt tgtcagttt gtttgcgag ttaaaaaag aagcacaaa cacggtaac aaggaggtt ttggccgcg ggagggcgc tacgacgct atgataaca tcgtaccgt aaaataatg gaaaatgcg aacatgtta gccagggat attaaacaa tcagctgtt ggtgtttcc ctctctttg gacaagttg gcccaggca gtgatgagg gaacaggag gatttgccc tcacttgag gtgctggac gttgcggtt tgttcagtt ggcaccaaa ccgctcctc agggagaaa actaagatt ttgcgcaat ttgtggtcg gccgggatt cgctgtgcc atgattgag gcaacaaat attgaagag attcaggaa cagtgtggc gagttaaaa gtgcctcac gttgttatg ttgaaagac agcgagcaa gggactgtg cgtgtgcgc agttgggaa cgcgatcgc tttcaggag aaaacggtt aaaacgcca gaactggtc gaaaccatg caacgaatc atcaagtgc tggaaggag ggggcgcag gaggtgctc ccaatcact ggaactgtc ttcaggtca gatagtaaa agcagttat gacaaaaat gagcacgag gccaccgtt gccgtcttg tttgttacg gaggataag ctgtcagcc aatgcgaga cggcgctac gatattcaa atccgatct catttagac gttttggtg aagaaattg agcggtttt gtgactgtt ttggggctc agtgtcgaa tcaaatgtc attcggacc atagcttcg tacctggag tgggaaacc gaccaacag tttcacaaa tcggtccaa gccataatc gagaagcac cagcgccac aaaaaatac ctaactgac gtctgtgat gaaatatac gaacagaaa agcaaaaag acgaaacct gcggtgatc ctgttcagt ctgctcgac tcgttttat aggttgtta atttag
- >TC009201 RA
caactccag tcggaatca tgtcctcgg aagagtcaa cagagtccc ggcaaaaca acgagttgg aagcgctcc aggccattt acggcgacg acttgaggg acctgcgag gaaaatcgg cctggaacg cctggaccc ctttaaatt tatcaatct ctctgaacc cccaacagg gcagttcgg gcctccacg aagtctacg tcaagcttg atttgcacg tcacgtgcc ccgaaacgt accccaaca ctgttcctg tgattaaat tagagaaca gtaaaggcc tgtccgaca attccctca tccagctcc agaaaatcc tagaggaaa aggcggaca agttaaggg gcgaggaga tgatttttc agttggcgc agcacgtcc aggagttcc tccacttgc acaacaaac cggcgtcga agagcttct acgacgaga tgctccaga gacagaagg aaaaggagg agaaggatt tacaagcca aactaatgg aggaagata gagaaaggc aattcgtac aacaagaag tacaaaagc ggcaagaaa ttttgaaat tggagtcgc gcatgcgcc gttacaatt ccgaaaact ccgacgaca gtgatcaaa ggcggcgct cgatctcgt acaactcga atgaaaaca gcgatgagt ctctctgta gccacaaaa agaccgtaa ttgtggaat tcaccaatc gtgagatca agcgaggcc agtgcttaa aacattgca gctacaatc acgtggttt ttatcggaa tggacgtca agaccggcg aaattgtcc ttttgagcg agtggatca tcaccaatc gtaaatatc tgtcgagtc ttgagcaag aatttaatt atttggcca aactgaagc accaaaacc tcgcccact atttgagtt ttaagtacg aagggcatg cagatggcg ctgttgttc atattttga aagagtatg tcagtgggc ccaactgtt cgtttttca tgaatgaga atctcagtg tgagcatcc agtttatta aatatgtgg cgaagggtg tgctcactg ctctcgatt atttgcata ggaataacg tcgtgcata aagacataa gagagagtt gtgtgtaca tcagtgaga aaggcattg ttgcgctga ctaattata gtttgcatc gcagactga ctgatatct cctcacaaa cgaacatta cctccaatt atagcaaaa agactgata tttataaat ttggcgtgt tcttaattt cactattgc aaggcgcga gcttaacag aggacgatg atgtcgata ttccgcctt gcgttccat cagatctct atgattttt tatcaagat gtttggaaa agcaggaaa aagagcgtt ttaccgcag tccaattat taaatcatt cgtttcttc gccagaaca gtttattat cgtcacctg gtcacaaaa tcgaccata atattccag atgggaaca tttcgcctg atttggcaa ccgatgaac ttaatccat tgcagcatc aaagtggcc agtctcgca ttgagaaag agtttgaaa ttatcgata atttgggca agggggcgt atggggacg ttattaagg ctaggaata atttagatg ggagatatt acgcaatca aacgcatta aattaaacc ccaggaacc gacaattaa ataaaaaaa ttgttcgtg aagttaagc tcttatcga ggctaaatc atgaaaata tagtgcgtt attataatt cctggattg aacggacgg tgataaagg gtgacgaca gcagtgata tggaaagta caacgtcgg ggattgacg ataagtgtc caaggatag tccgtccag atgagttca ccctcaatg acaatattg aagagctgg cgccctcta taaaaaacg tggaagttt ccgtaacgt ttgacagca tatcacagc ctgcttacg actcgtcat cggatgaat caagctgtg atgacgaga acagcctgg acatttacg aggaatcag attcggacg gaatcgagt tcgaaaaag actcacatg cccccagcc ccagcgacc ccccgtcca ccaaaagcc cccaactgc tcgaaacgg ggcgccaaa tcgaattca tgtacatcc agatggagt tttgcgaaa agagcaccc tacgtaacg ccatagata acagtgtgt acctagacg aggaccgag tgtggcgcc tcttccgcg aaatagttg aaggcttag cccacatcc accagcaag gcatgatcc atcgtgacc tcaaacccg tcaatattt tcctcgatt cgaacgatc acgtgaaaa tcggcgatt tcggattgg ccacgaaca tgacaccga agctaaacg agcacgatt tgaaccgaa cctttccgg aacggtacg acatggacg agagcaaga cgggattgg tgggcacag cactctacg tggcgccgg aattaaacg tcgcctcca aggcgaatt acaaccaaa aggttgata tttacagtt tgggcatta tttttttcg aaatgtgtt acaagccct tgttgacca acatggaaa ggatcaaga ttttgacga gtttgcgga agaaagaga tcgtgttac ctgaggatt tcgcaccgg ggaatccca aacagaagt ttctggtca cgtggttgt tgaatcatg acgttgcga aacggccaa cttcggtcg agttgctac aatcggaac atataccgc cccctgttt tggaagaat gtgaattaa gagaactgg ttaggcaca ctttaagta acccacaat tgaaaggtt acaagtact tgatagcat catgttttg atcagacgt taacaccgg cacaaaaca taacgtatg ataaggatt cagcagtgc caactttaa ataaaccac aaatttatg actttgtca aagaaacaa tagtcaaga ttttcaaac aacatgggg cccagaatt tgacaacgc ctcttttaa tgcccaaaa gtcactact acgagggcg tggagtctt gtgtcaaac ttatgacac actttggag gtattgttt cgctcccgc atgacttga gggttccgt ttgcccgtt atgtggcct ggaaccgaa tcacccttt tacgtcgct actcagtcg agcgtgttt atcgcgaaa aacgcgttt tcgggtttt taccgcgtg aattgtacg agtgcgcct ttgatatcg tcaccccga gcccgggga atcacacat cagacgccg aagttttgt acgtcattt acgaaatca tcaacgaac ttccggggg tgaagaaca aacacgtca cgatccgac tcaaccatt cgctgctga tgaaggcga ttctgttgc attgtggcg tcaagaacc gacacgagg agattttac agaaattct ccgatgtta aagacggta aagtaccga aaagcgacc tgcaagact acctgatcc agttcggcc tatccgaca atgcaattt cgaccctaa ctaacattt ttagttcgg agtttgagg tcagcaagg ccgcaaacc acttccagt caatcactc ggaaaaaat cgagcgagg cgagccatt tggccaagc aggcgttgc aagaattga aaagtatag catacaatg cggaggcgt tcggagtca cgtttgaaa tggtggtgg cacccggtg ccgtgtaca acatccaac aatattccg gaatgattt gtcagtttg tttgcgagt taaaaaaga agcacaaac acggtaaca aggaggttt tggccgcgg gagggcgct acgacgcta tgataacat cgtaccgta aaataatgg aaaatgcga acatgttag ccagggata ttaaacaat cagctgttg gtgtttccc tctctttgg acaagttgg cccaggcag tgatgaggg aacaggagg atttgccct cacttgagg tgctggacg ttgcggttt gttcagttg gcaccaaac cgctcctca gggagaaaa ctaagattt tgcgcaatt tgtggtcgg ccgggattc gctgtgcca tgattgagg caacaaata ttgaagaga ttcaggaac agtgtggcg agttaaaag tgcctcacg ttgttatgt tgaaagaca gcgagcaag ggactgtgc gtgtgcgca gttgggaac gcgatcgct ttcaggaga aaacggtta aaacgccag aactggtcg aaaccatgc aacgaatca tcaagtgct ggaaggagg gggcgcagg aggtgctcc caatcactg gaactgtct tcaggtcag atagtaaaa gcagttatg acaaaaatg agcacgagg ccaccgttg ccgtcttgt ttgttacgg aggataagc tgtcagcca atgcgagac ggcgctacg atattcaaa tccgatctc atttagacg ttttggtga agaaattga gcggttttg tgactgttt tggggctca gtgtcgaat caaatgtca ttcggacca tagcttcgt acctggagt gggaaaccg accaacagt ttcacaaat cggtccaag ccataatcg agaagcacc agcgccaca aaaaatacc taactgacg tctgtgatg aaatatacg aacagaaaa gcaaaaaga cgaaacctg cggtgatcc tgttcagtc tgctcgact cgttttata ggttgttaa tttaggtaa attattttt ttacatgta ttgtttgat aagattaca atcttagt
