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Gene information
General information
TC009615 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3267976..3269558
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009615
    iBeetle screen iB_08663

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009615 RA
      MSCNHCNTK FNFFHKEMG CSNCGLSFC NKCLKQKCK 
IPNKGTGEF NVCKLCYSK LASGTSATL PQIAPPDAF 
IKRLENLEN PAAPPITMY KNDPKIQAL RSGLSPADQ 
KILDRLEKL KDEKGPPPS EGELRRRLA SLKGENDYV 
EGPSRLLFQ VDSRTDQQK ADSLLEQFV NERDIELAH 
NPQEEIEAR LATLREQGV RPNEGPYIS NLHDSSSSE 
EEVDKITKK IMDEVAIEE RCPKSKKLT PSSSNEDEN 
NVRSSPELP WCVLCNNDA KFRCFDCGG DLYCSECNV 
EVHKNWGDT DHKVVPYKS K
    CDS sequence
    • >TC009615 RA
      atgtcttgt aatcactgc aatacgaaa tttaatttt 
ttccacaag gaaatgggc tgttcaaat tgtggactg 
tccttttgc aataaatgt ctgaagcaa aaatgcaaa 
atccccaac aagggcaca ggcgaattc aatgtgtgc 
aaactgtgt tactccaaa ctggcatca gggacaagc 
gcgacactg ccccaaatc gcgcccccc gacgctttc 
ataaagcga ttggaaaat ctggaaaac ccggccgcg 
ccccctatt acaatgtac aaaaacgac ccgaaaatc 
caggcttta cgcagcggt ttgtcgccg gctgaccag 
aaaattctg gatcggttg gagaagctt aaagatgag 
aaggggccc ccgcccagt gagggggag ctaaggagg 
cggttggcc agtcttaag ggggagaac gactacgtg 
gaggggccc agcagactt ttgtttcaa gttgattca 
aggacggat caacagaaa gctgactcg ctccttgaa 
cagtttgtg aatgagagg gacatcgag ttggctcac 
aacccccag gaggagatt gaggcgcgt ttggcgact 
ttgagggag caaggagtg agaccaaac gagggacca 
tatattagt aatttgcac gactctagt agctctgag 
gaggaagtg gataaaatc acgaaaaag atcatggac 
gaggtggcg attgaggag cgttgcccc aaatcaaaa 
aaactcact ccaagttcg tcaaacgaa gatgaaaac 
aacgtacga tcgtctccg gagcttccg tggtgcgtt 
ttgtgcaac aacgatgcg aaattccgg tgttttgat 
tgtggaggt gatttatac tgtagcgag tgtaatgtt 
gaagtacac aaaaactgg ggagatacc gatcacaaa 
gttgttcca tacaagtcg aaatga
    mRNA sequence
    • >TC009615 RA
      aataagtcg ctttttgca gcaaatggg tgcaaatca 
aatttttcc atgacgtgc cttcagcca cttgcagcg 
aatcaattc agctgtcac aaaagtcta aatttgtaa 
aaaatctct ttaattcta ttatgtctt gtaatcact 
gcaatacga aatttaatt ttttccaca aggaaatgg 
gctgttcaa attgtggac tgtcctttt gcaataaat 
gtctgaagc aaaaatgca aaatcccca acaagggca 
caggcgaat tcaatgtgt gcaaactgt gttactcca 
aactggcat cagggacaa gcgcgacac tgccccaaa 
tcgcgcccc ccgacgctt tcataaagc gattggaaa 
atctggaaa acccggccg cgcccccta ttacaatgt 
acaaaaacg acccgaaaa tccaggctt tacgcagcg 
gtttgtcgc cggctgacc agaaaattc tggatcggt 
tggagaagc ttaaagatg agaaggggc ccccgccca 
gtgaggggg agctaagga ggcggttgg ccagtctta 
agggggaga acgactacg tggaggggc ccagcagac 
ttttgtttc aagttgatt caaggacgg atcaacaga 
aagctgact cgctccttg aacagtttg tgaatgaga 
gggacatcg agttggctc acaaccccc aggaggaga 
ttgaggcgc gtttggcga ctttgaggg agcaaggag 
tgagaccaa acgagggac catatatta gtaatttgc 
acgactcta gtagctctg aggaggaag tggataaaa 
tcacgaaaa agatcatgg acgaggtgg cgattgagg 
agcgttgcc ccaaatcaa aaaaactca ctccaagtt 
cgtcaaacg aagatgaaa acaacgtac gatcgtctc 
cggagcttc cgtggtgcg ttttgtgca acaacgatg 
cgaaattcc ggtgttttg attgtggag gtgatttat 
actgtagcg agtgtaatg ttgaagtac acaaaaact 
ggggagata ccgatcaca aagttgttc catacaagt 
cgaaatgaa atttcgtag atttagagc aagttacat 
atttttaca acggatttt caatatttt tgcttaaca 
ttcccggaa ttaaaaaat cgttgatgt gtcgagatt 
tagcggtga atgtggata tattgtacg aagaaattt 
aatcaatgt catgtaaat aaagatttt aagagatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY