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Gene information
General information
TC009617 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3279166..3280230
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009617
    iBeetle screen iB_08661

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009617 RA
      MAQKLSPDM FRSAFKAID KDVVRWFPG HMGRGLKQM 
      QHKLRSIDC IIEVHDARI PISGRNTDF KYTISGIKP 
      HILVLNKVD LIDKKLIPS IKNRLKNEF ENVVFTNCK 
      DQQCKGVKT LFPLAQNLI KNSDRFNRS QAEDQCVMI 
      IGVPNVGKS SLINALRVR NLHKGNATH VGATPGITR 
      SVLTKIKMS EDPLCYMLD TPGILTPKI ESAEVGLKL 
      ALCATIQDH LVGEGVIAD YLLFWLNKN GHYNYVKFF 
      GLEKATDDI LEVLAQISV KKKKMLKLR DNTNNYVYK 
      PDLDYGAKV MLQAFRKGD LGKIVLDED LL
    CDS sequence
    • >TC009617 RA
      atggcccaa aaactgtcg cctgatatg ttcaggagc 
      gccttcaag gcgatcgac aaggacgtg gtacgttgg 
      ttcccgggg cacatgggg cgcggcctt aaacaaatg 
      cagcataaa cttcgctca atcgactgc attattgaa 
      gtccacgac gcaagaatc ccgatttcg ggccgcaac 
      accgacttc aaatacaca ataagtggc ataaaaccc 
      cacatccta gtcttaaac aaagtcgac ctaatcgac 
      aaaaaatta atcccttcg ataaaaaat cgtctaaaa 
      aatgagttc gaaaacgta gttttcacg aactgtaag 
      gaccagcaa tgtaaaggc gtaaaaaca ctcttccct 
      ttggctcaa aatttgatc aaaaactct gataggttc 
      aatcgtagt caggctgag gatcagtgc gttatgata 
      ataggggtt ccaaacgtg ggcaaatca tcgctaatc 
      aatgcccta agggtgcgg aatttgcac aaggggaat 
      gccacccac gtgggtgcc acaccgggg attacccga 
      agtgtttta accaaaatc aaaatgagt gaagacccg 
      ctgtgttac atgcttgac acaccaggg attttaaca 
      ccgaaaatt gagagtgct gaagtgggg ctgaagctg 
      gctttgtgt gctacaatt caggaccat ctagttggg 
      gagggtgtg attgctgat tacttgctt ttttggtta 
      aataaaaac ggacactat aattatgtg aagttcttc 
      gggcttgag aaagccacc gatgatatt ttggaagta 
      ttagctcag attagtgtt aagaagaaa aaaatgtta 
      aaattgagg gacaatacc aataattat gtctataaa 
      ccagatttg gattatggg gccaaagtg atgttgcag 
      gcgttcagg aagggggat ctgggcaag attgtgcta 
      gacgaagac ttgctataa
    mRNA sequence
    • >TC009617 RA
      cttattaaa aaaaaaatt acataaccc caaaattga 
      ggttaaata cgtcttgtg aagtgggct aaagttgtg 
      aaaatggcc caaaaactg tcgcctgat atgttcagg 
      agcgccttc aaggcgatc gacaaggac gtggtacgt 
      tggttcccg gggcacatg gggcgcggc cttaaacaa 
      atgcagcat aaacttcgc tcaatcgac tgcattatt 
      gaagtccac gacgcaaga atcccgatt tcgggccgc 
      aacaccgac ttcaaatac acaataagt ggcataaaa 
      ccccacatc ctagtctta aacaaagtc gacctaatc 
      gacaaaaaa ttaatccct tcgataaaa aatcgtcta 
      aaaaatgag ttcgaaaac gtagttttc acgaactgt 
      aaggaccag caatgtaaa ggcgtaaaa acactcttc 
      cctttggct caaaatttg atcaaaaac tctgatagg 
      ttcaatcgt agtcaggct gaggatcag tgcgttatg 
      ataataggg gttccaaac gtgggcaaa tcatcgcta 
      atcaatgcc ctaagggtg cggaatttg cacaagggg 
      aatgccacc cacgtgggt gccacaccg gggattacc 
      cgaagtgtt ttaaccaaa atcaaaatg agtgaagac 
      ccgctgtgt tacatgctt gacacacca gggatttta 
      acaccgaaa attgagagt gctgaagtg gggctgaag 
      ctggctttg tgtgctaca attcaggac catctagtt 
      ggggagggt gtgattgct gattacttg cttttttgg 
      ttaaataaa aacggacac tataattat gtgaagttc 
      ttcgggctt gagaaagcc accgatgat attttggaa 
      gtattagct cagattagt gttaagaag aaaaaaatg 
      ttaaaattg agggacaat accaataat tatgtctat 
      aaaccagat ttggattat ggggccaaa gtgatgttg 
      caggcgttc aggaagggg gatctgggc aagattgtg 
      ctagacgaa gacttgcta taacttgta gattattat 
      aaataaatt tttatagaa gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY