| General information | |||
| TC005184 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 4619143..4626964 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC005184 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08660 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Days post injection
Adult morphology
abdominal odoriferous glands colour lighter -
Number of animals showing the phenotype: 5
(13 dpi)
Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature pattern irregular -
Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature randomnot present - Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature randomrounded - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature randomnot present - Penetrance (percentage of animals/eggs on slide): more than 80%
larval musculature randomrounded - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
antenna bent - Penetrance (percentage of animals/eggs on slide): more than 80%
legs bent - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen posterior circumference decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
antenna bent - Penetrance (percentage of animals/eggs on slide): more than 80%
legs bent - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen posterior circumference decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005184 RA
MFRPNSAIW TLLAFILFQ VVQSNSQVF GQSYSRIRG NPSGGYVPV KLHPCEQSS CYPATGNLL IGREHQLYA SSTCGLHGP QRYCIVSHL EERKKCFRC DSRPTNKPN PLFNHNISN IVYRMYPGT RQKSWWQSE NGKEGVYIQ LDLEAEFHF THLIITFKT FRPAAMLIE RSYDFKQTW HVYRYFAYN CAESFPHIR EGYPVNLTD VVCESRYSS IDPSTDGEV IYRVLPPTL HIDNPYSEQ VQNLLKMTN LRINFTKLH TLGDDLLDK REDIQEKYY YAITDMVVR GSCSCYGHA NRCLPLPGI EPKPDMVHG RCECTHNTK GRNCEKCED FFNDLPWRP AIGKQTNAC KKCNCNNHA TSCHFDAAL YEATGRISG GVCDGCQHN TMGPNCEQC KPFYYRDPQ RDIQDPEVC RPCDCDPRG SLDNGICDS ITDAENNLI AGSCHCKTN VEGRRCDIC KNGFWNFTE SNPDGCQSC TCNTFGTID NQGCNVYNG ECTCKRYVT GRDCNQCLP EYWGLSEKR DGCQPCDCD PGGSFDNNC DVITGQCLC REHMTGRTC SIPKQQHYT ASLDFLLYE AESARASPN CQVVIREPP RDGSDVTWT GIGFMKAFE PSYIEFTVD NIKTGMDYE IVIRYEPTM PTNWEEVEV TLERPGPVD PNGPCNGAR DDPRRVALP SNSRSVTVY PPVCLEPGR TYKIRLTFR RSNFERETP SASVLIDSV VLIPRIEKI PWFYGSVPA EARRQEFER NRCSSYSSY LIHTGQIPE VCKKYYSSI GAYIFNGAF SCQCDPTGS LSKLCDEYG GHCTCKLNV VGRRCDQCA PGTYGFGPE GCKSCDCNS IGALDNFCN ATTGQCKCR ANTYGRECD QCRTGFWNF PNCQRCDCN GHADICDSK TGACINCKD NTEGHNCEQ CVDGFYGDP RLNVDIPCR PCPCPGSPW SNHSYADRC SLEASTKDV ICECHVGYS GSRCDVCSD NYFGNPEEP GGLCQPCDC NDKIDILKP GNCDPHTGK CKQCLYDTT GDHCEVCRP GFFRYSDDM MCEECVCHP LGTNSSAGP CDPSSGQCV CLPNVTGLN CDQCIANHW KIASGEGCE PCNCDPQGS LRPQCNLFD GQCECRPGF GGRQCNECV ARFWGDPKV ECRECKCDA RGSRSLQCD MRTGACKCL PGIGGYNCD RCDRGYLGQ APECIPCGE CFDNWDRIL QETRNQTMD LINRAGDIK KVGATGAYT KEFDDMQFQ LDEIKQLLS NTHEVDIDA IDKELQDLR TKINETELG SVKELDDSI ATTKENMLL TDLKLKSLK QRIDDFKNK TKELEMNGT QLQEANVQG ALTLIQNAK VKADLAAHK AERTEETIN YAERQIKAT ESTINETAH NFNQQLKNN EKKLIDLNE KLDSLKQTL PDLNEQICD GRGDPCDSL CGGAGCATC GVSISCENG AKQQAETAL AIANRTEMA LREKEAMAN DFIRNVSQI NTNETRNIA QETYDKAKG GLMKANDSL NNVLNLVKE VNEFMDRND TKEDIKNFT QQILAMKIE LEPDEITNM ANKIKEAVQ HLTDIDPII EATKDDLRR VNRLRENAE YAKTNASKI LTDAADVTS TLENTTIAQ DNAQSAIDT AWNDINIVN DRLKQIGSK TQSAQKKTN NTADDLKKL EEQLNQLQR NITDNGIYA NRVVNESSN ILEKARTTY DDFNELQAQ YSKVQRNLT TSLDKVQSS KDRANDLFK RAWELVAKV TSTEEEISK LENSSQQAT LTNLENILQ DLIRRMNGY NQKIQERVH YYKQCN
- >TC005184 RB
MFRPNSAIW TLLAFILFQ VVQSNSQVF GQSYSRIRG NPSGASVKR GDQPPDIDS EYPDGDVLH PLTDVFNRR PYTHNHGYV PVKLHPCEQ SSCYPATGN LLIGREHQL YASSTCGLH GPQRYCIVS HLEERKKCF RCDSRPTNK PNPLFNHNI SNIVYRMYP GTRQKSWWQ SENGKEGVY IQLDLEAEF HFTHLIITF KTFRPAAML IERSYDFKQ TWHVYRYFA YNCAESFPH IREGYPVNL TDVVCESRY SSIDPSTDG EVIYRVLPP TLHIDNPYS EQVQNLLKM TNLRINFTK LHTLGDDLL DKREDIQEK YYYAITDMV VRGSCSCYG HANRCLPLP GIEPKPDMV HGRCECTHN TKGRNCEKC EDFFNDLPW RPAIGKQTN ACKKCNCNN HATSCHFDA ALYEATGRI SGGVCDGCQ HNTMGPNCE QCKPFYYRD PQRDIQDPE VCRPCDCDP RGSLDNGIC DSITDAENN LIAGSCHCK TNVEGRRCD ICKNGFWNF TESNPDGCQ SCTCNTFGT IDNQGCNVY NGECTCKRY VTGRDCNQC LPEYWGLSE KRDGCQPCD CDPGGSFDN NCDVITGQC LCREHMTGR TCSIPKQQH YTASLDFLL YEAESARAS PNCQVVIRE PPRDGSDVT WTGIGFMKA FEPSYIEFT VDNIKTGMD YEIVIRYEP TMPTNWEEV EVTLERPGP VDPNGPCNG ARDDPRRVA LPSNSRSVT VYPPVCLEP GRTYKIRLT FRRSNFERE TPSASVLID SVVLIPRIE KIPWFYGSV PAEARRQEF ERNRCSSYS SYLIHTGQI PEVCKKYYS SIGAYIFNG AFSCQCDPT GSLSKLCDE YGGHCTCKL NVVGRRCDQ CAPGTYGFG PEGCKSCDC NSIGALDNF CNATTGQCK CRANTYGRE CDQCRTGFW NFPNCQRCD CNGHADICD SKTGACINC KDNTEGHNC EQCVDGFYG DPRLNVDIP CRPCPCPGS PWSNHSYAD RCSLEASTK DVICECHVG YSGSRCDVC SDNYFGNPE EPGGLCQPC DCNDKIDIL KPGNCDPHT GKCKQCLYD TTGDHCEVC RPGFFRYSD DMMCEECVC HPLGTNSSA GPCDPSSGQ CVCLPNVTG LNCDQCIAN HWKIASGEG CEPCNCDPQ GSLRPQCNL FDGQCECRP GFGGRQCNE CVARFWGDP KVECRECKC DARGSRSLQ CDMRTGACK CLPGIGGYN CDRCDRGYL GQAPECIPC GECFDNWDR ILQETRNQT MDLINRAGD IKKVGATGA YTKEFDDMQ FQLDEIKQL LSNTHEVDI DAIDKELQD LRTKINETE LGSVKELDD SIATTKENM LLTDLKLKS LKQRIDDFK NKTKELEMN GTQLQEANV QGALTLIQN AKVKADLAA HKAERTEET INYAERQIK ATESTINET AHNFNQQLK NNEKKLIDL NEKLDSLKQ TLPDLNEQI CDGRGDPCD SLCGGAGCA TCGVSISCE NGAKQQAET ALAIANRTE MALREKEAM ANDFIRNVS QINTNETRN IAQETYDKA KGGLMKAND SLNNVLNLV KEVNEFMDR NDTKEDIKN FTQQILAMK IELEPDEIT NMANKIKEA VQHLTDIDP IIEATKDDL RRVNRLREN AEYAKTNAS KILTDAADV TSTLENTTI AQDNAQSAI DTAWNDINI VNDRLKQIG SKTQSAQKK TNNTADDLK KLEEQLNQL QRNITDNGI YANRVVNES SNILEKART TYDDFNELQ AQYSKVQRN LTTSLDKVQ SSKDRANDL FKRAWELVA KVTSTEEEI SKLENSSQQ ATLTNLENI LQDLIRRMN GYNQKIQER VHYYKQCN
- >TC005184 RA
atgttccgc ccaaattcg gcaatatgg acactttta gcctttatt ttattccaa gtagtacaa agtaattca caagtattc ggtcaatcg tactcgcgg ataagagga aacccctca ggagggtac gtgccggtc aagctacat ccttgtgag caaagctcg tgctatcca gccactgga aacctacta ataggtcgg gaacatcag ttgtacgcg tcttccact tgcggcctc cacggccca cagagatac tgtattgtg tcgcatttg gaagagcgg aagaagtgt tttagatgc gattccagg ccgaccaac aaaccaaat cctttgttt aatcacaac attagtaat attgtctat aggatgtat cctgggacg cggcagaag tcgtggtgg cagtccgaa aacggcaaa gagggtgtc tacatccag ttggatcta gaagccgag tttcacttt acgcatctt attatcacg tttaagact tttaggcct gcggctatg ttaatcgag cgttcgtac gatttcaaa cagacgtgg catgtctat cgctacttt gcctacaac tgcgctgaa agttttccg catattagg gaaggatat cctgtgaat ctcactgat gttgtttgc gaatcgcgc tattcgtcg atagacccg tcaacggac ggtgaagtg atttaccgc gtgttgccc ccaactctc cacattgac aacccgtac tcggaacaa gtccagaac ttgctcaaa atgaccaat ttgcggatc aatttcacc aaattacac actctgggt gacgacctc ctcgacaaa cgcgaggac atccaagag aagtactac tacgccatc accgacatg gtggtgcgc ggctcctgt tcgtgctac ggacacgct aataggtgt ctaccactg ccggggatt gagcccaag ccggacatg gtgcacggc cgctgcgag tgcacgcac aacaccaaa ggccgcaat tgcgaaaag tgtgaggac tttttcaac gacttgccg tggcggcct gccatcggc aaacaaacc aacgcgtgt aaaaaatgc aactgtaac aaccatgca actagctgc catttcgac gccgctctc tacgaagcc accggtcgc atcagtggt ggcgtttgc gacgggtgc cagcacaac accatgggc cccaactgc gagcagtgc aaacctttc tattacagg gacccgcag cgggacatc caggaccct gaagtgtgt cggccctgt gattgcgac cctcgcgga tcgcttgat aacggcatt tgcgactct atcaccgat gctgagaat aatctaatt gctggaagt tgccactgt aagactaat gtggaaggc cgccggtgc gatatttgc aagaacggc ttttggaac tttacggaa agtaatcca gatgggtgt cagagttgc acttgcaac acttttgga acaattgac aatcagggt tgtaatgtg tacaatggg gagtgcact tgtaagagg tatgtgact gggcgcgat tgcaaccaa tgtctcccc gagtattgg gggctttcg gaaaaacgc gacggttgc cagccgtgc gattgtgat cctggaggt tcgttcgat aacaattgc gacgttatc actggacag tgtctctgc cgagaacat atgacgggg cggacgtgt agcatcccc aaacagcaa cactacacc gcaagtttg gacttccta ttgtacgaa gctgagtcg gcgagggcg tcacctaac tgtcaagtc gtaataagg gagcccccg cgggacggg tccgatgtt acatggacc gggatcggg tttatgaag gcttttgag ccgtcgtac attgaattc accgtggat aacatcaag acgggcatg gactacgaa atcgtgatc cgctacgaa cccacaatg cccacaaac tgggaggag gtcgaagtg accctcgag cggccaggc cccgttgat ccaaacggc ccttgcaac ggagcccgc gatgatccc agaagagtc gcactaccg tcgaacagc agaagcgtc actgtgtac ccccctgtg tgtctggag cccggccga acctacaaa atccggctg actttccgc cggtctaac ttcgaacgc gagacaccc tcagcttcg gtcttaata gactcggtc gtgttaatc ccccgcatt gagaaaatc ccctggttc tacggctcg gtgcccgcc gaggcccgg cgccaagaa ttcgaacgc aaccggtgc agctcctac tcctcctac ctaatccac accggacaa atccccgaa gtttgcaaa aagtactac agcagcatt ggcgcttac atcttcaac ggcgccttc tcctgtcaa tgcgacccg accggctct ctcagcaag ctttgcgac gagtatggg ggccactgc acctgcaaa ctgaacgtg gtcggccgc cggtgcgac cagtgcgcc cccgggact tacggcttc gggccggag ggttgcaag tcttgcgat tgcaacagt atcggggcc cttgacaat ttctgcaat gccaccact ggacagtgc aaatgccgg gccaacacg tacgggcgg gagtgcgac cagtgcagg accggcttt tggaatttc cccaactgt cagaggtgc gactgtaac gggcatgcg gatatttgt gattccaaa accggggct tgtatcaat tgtaaggac aacacggag gggcacaat tgcgagcaa tgcgtcgac gggttttac ggggatccc agacttaac gtggatatt ccgtgccgg ccgtgtccc tgcccgggg agcccctgg tcgaatcac tcctacgct gataggtgt agcctggaa gcgagcacc aaggatgtg atttgtgag tgtcatgtg gggtattca gggtcacgt tgtgacgtc tgttctgat aactatttc ggaaatccg gaagaaccg ggcggtttg tgccaaccg tgcgattgc aacgataaa attgatatc cttaaaccg ggaaattgt gacccacac accggtaaa tgcaagcaa tgtttgtat gatacgacg ggagaccac tgcgaggtg tgtagaccc ggctttttc cgatattcc gatgacatg atgtgtgaa gagtgtgtc tgtcaccca ttgggcacg aacagctca gccgggcct tgtgaccca tcttccggg caatgcgtc tgtcttcca aacgtgacc ggattaaat tgtgaccaa tgtatcgcc aaccattgg aaaattgca agtggggag ggttgtgaa ccttgcaat tgtgacccg caaggctca ctacgacca cagtgcaac ttgttcgat gggcaatgc gaatgtcgg ccaggtttt ggtggcaga cagtgtaac gaatgtgtg gccagattc tggggagac cccaaagtt gaatgtcgc gaatgtaaa tgtgacgca agagggtct aggtctttg cagtgtgac atgagaact ggtgcttgt aagtgtttg ccagggata ggtgggtat aactgcgac aggtgtgat cgaggctac ttgggacaa gcacctgag tgtatccct tgtggggaa tgtttcgat aattgggac cgcattttg caagaaacg cgaaaccaa acaatggat ttaattaac cgtgccggt gatatcaag aaagttggc gcaactggc gcttacacc aaagaattc gacgatatg caattccaa cttgacgaa ataaaacaa ctgttgagt aacacccac gaagtcgat attgacgcc attgataag gaattgcaa gacttacgt acaaaaatc aacgagact gagcttgga agcgtcaaa gaacttgat gattcaatc gccacaacc aaagaaaat atgctacta accgactta aaactgaaa agtttgaaa caacgaatc gacgacttt aaaaacaaa acaaaagaa ctggaaatg aacggtacg caactacaa gaagccaac gtccaaggg gcccttacc ctaatccaa aatgccaaa gtcaaagct gatctcgca gcgcataaa gcggaaaga accgaagaa actataaat tatgccgaa cgtcagatt aaagccact gaaagcacg attaacgaa actgcacat aacttcaac caacagctg aagaacaac gaaaagaag ctcatcgac ttgaacgaa aaacttgac agtcttaaa caaaccctt cccgatttg aatgagcaa atttgtgac ggcagaggc gacccttgt gactcgttg tgtggcggc gcaggttgt gccacttgt ggtgtgtca atttcgtgt gaaaatggg gccaaacaa caggctgaa accgcgcta gctatagcg aatcggacg gaaatggcc ctccgtgag aaggaagca atggctaat gatttcatc cgaaatgtc tctcaaata aacacaaat gagacgagg aatatcgca caagagacg tacgataaa gccaaggga gggttaatg aaagctaat gactcgttg aataatgtt ttgaactta gtgaaggaa gttaacgag tttatggac cgcaatgac accaaggaa gatataaaa aatttcacg cagcagatt ttggccatg aaaattgaa ctcgaaccg gacgaaatc acaaacatg gctaataaa atcaaagaa gcggtccaa cacttgact gatattgac cccattatt gaagctacc aaagatgat ttacggaga gttaacaga cttagggaa aacgctgaa tatgccaaa acgaatgcg tcaaaaata ttgaccgat gccgccgac gtgactagt actttagaa aacacaaca atcgcgcaa gataatgcc caaagcgca attgacaca gcctggaat gacatcaac attgtgaac gatcggtta aaacagatt ggctcgaaa acacagtcg gcccaaaaa aagacaaac aatacagcc gacgacctc aaaaaactc gaggaacaa ctcaatcag cttcaacga aacataacc gataacgga atctacgcc aatcgtgtc gtaaacgaa tcgtcaaac attttggaa aaagcccgg acaacttac gacgacttc aacgaactc caggcacag tattccaaa gttcaacgc aatttaaca acgagttta gacaaagtt cagtcgtcg aaagaccgc gctaatgat cttttcaag cgtgcgtgg gaacttgtt gccaaagta acgagtact gaagaggaa attagcaaa ctggagaac agttcccaa caagcgact ttaactaat ttggaaaat atactacaa gatttgata aggcggatg aatggctac aatcaaaaa atacaggag agagtgcat tactacaaa cagtgtaat taa
- >TC005184 RB
atgttccgc ccaaattcg gcaatatgg acactttta gcctttatt ttattccaa gtagtacaa agtaattca caagtattc ggtcaatcg tactcgcgg ataagagga aacccctca ggagcttca gtaaaaagg ggtgaccaa ccgcctgat atagatagc gagtacccc gacggcgac gttcttcat cccttgacg gatgtcttc aatcgccga ccttacact cataatcat gggtacgtg ccggtcaag ctacatcct tgtgagcaa agctcgtgc tatccagcc actggaaac ctactaata ggtcgggaa catcagttg tacgcgtct tccacttgc ggcctccac ggcccacag agatactgt attgtgtcg catttggaa gagcggaag aagtgtttt agatgcgat tccaggccg accaacaaa ccaaatcct ttgtttaat cacaacatt agtaatatt gtctatagg atgtatcct gggacgcgg cagaagtcg tggtggcag tccgaaaac ggcaaagag ggtgtctac atccagttg gatctagaa gccgagttt cactttacg catcttatt atcacgttt aagactttt aggcctgcg gctatgtta atcgagcgt tcgtacgat ttcaaacag acgtggcat gtctatcgc tactttgcc tacaactgc gctgaaagt tttccgcat attagggaa ggatatcct gtgaatctc actgatgtt gtttgcgaa tcgcgctat tcgtcgata gacccgtca acggacggt gaagtgatt taccgcgtg ttgccccca actctccac attgacaac ccgtactcg gaacaagtc cagaacttg ctcaaaatg accaatttg cggatcaat ttcaccaaa ttacacact ctgggtgac gacctcctc gacaaacgc gaggacatc caagagaag tactactac gccatcacc gacatggtg gtgcgcggc tcctgttcg tgctacgga cacgctaat aggtgtcta ccactgccg gggattgag cccaagccg gacatggtg cacggccgc tgcgagtgc acgcacaac accaaaggc cgcaattgc gaaaagtgt gaggacttt ttcaacgac ttgccgtgg cggcctgcc atcggcaaa caaaccaac gcgtgtaaa aaatgcaac tgtaacaac catgcaact agctgccat ttcgacgcc gctctctac gaagccacc ggtcgcatc agtggtggc gtttgcgac gggtgccag cacaacacc atgggcccc aactgcgag cagtgcaaa cctttctat tacagggac ccgcagcgg gacatccag gaccctgaa gtgtgtcgg ccctgtgat tgcgaccct cgcggatcg cttgataac ggcatttgc gactctatc accgatgct gagaataat ctaattgct ggaagttgc cactgtaag actaatgtg gaaggccgc cggtgcgat atttgcaag aacggcttt tggaacttt acggaaagt aatccagat gggtgtcag agttgcact tgcaacact tttggaaca attgacaat cagggttgt aatgtgtac aatggggag tgcacttgt aagaggtat gtgactggg cgcgattgc aaccaatgt ctccccgag tattggggg ctttcggaa aaacgcgac ggttgccag ccgtgcgat tgtgatcct ggaggttcg ttcgataac aattgcgac gttatcact ggacagtgt ctctgccga gaacatatg acggggcgg acgtgtagc atccccaaa cagcaacac tacaccgca agtttggac ttcctattg tacgaagct gagtcggcg agggcgtca cctaactgt caagtcgta ataagggag cccccgcgg gacgggtcc gatgttaca tggaccggg atcgggttt atgaaggct tttgagccg tcgtacatt gaattcacc gtggataac atcaagacg ggcatggac tacgaaatc gtgatccgc tacgaaccc acaatgccc acaaactgg gaggaggtc gaagtgacc ctcgagcgg ccaggcccc gttgatcca aacggccct tgcaacgga gcccgcgat gatcccaga agagtcgca ctaccgtcg aacagcaga agcgtcact gtgtacccc cctgtgtgt ctggagccc ggccgaacc tacaaaatc cggctgact ttccgccgg tctaacttc gaacgcgag acaccctca gcttcggtc ttaatagac tcggtcgtg ttaatcccc cgcattgag aaaatcccc tggttctac ggctcggtg cccgccgag gcccggcgc caagaattc gaacgcaac cggtgcagc tcctactcc tcctaccta atccacacc ggacaaatc cccgaagtt tgcaaaaag tactacagc agcattggc gcttacatc ttcaacggc gccttctcc tgtcaatgc gacccgacc ggctctctc agcaagctt tgcgacgag tatgggggc cactgcacc tgcaaactg aacgtggtc ggccgccgg tgcgaccag tgcgccccc gggacttac ggcttcggg ccggagggt tgcaagtct tgcgattgc aacagtatc ggggccctt gacaatttc tgcaatgcc accactgga cagtgcaaa tgccgggcc aacacgtac gggcgggag tgcgaccag tgcaggacc ggcttttgg aatttcccc aactgtcag aggtgcgac tgtaacggg catgcggat atttgtgat tccaaaacc ggggcttgt atcaattgt aaggacaac acggagggg cacaattgc gagcaatgc gtcgacggg ttttacggg gatcccaga cttaacgtg gatattccg tgccggccg tgtccctgc ccggggagc ccctggtcg aatcactcc tacgctgat aggtgtagc ctggaagcg agcaccaag gatgtgatt tgtgagtgt catgtgggg tattcaggg tcacgttgt gacgtctgt tctgataac tatttcgga aatccggaa gaaccgggc ggtttgtgc caaccgtgc gattgcaac gataaaatt gatatcctt aaaccggga aattgtgac ccacacacc ggtaaatgc aagcaatgt ttgtatgat acgacggga gaccactgc gaggtgtgt agacccggc tttttccga tattccgat gacatgatg tgtgaagag tgtgtctgt cacccattg ggcacgaac agctcagcc gggccttgt gacccatct tccgggcaa tgcgtctgt cttccaaac gtgaccgga ttaaattgt gaccaatgt atcgccaac cattggaaa attgcaagt ggggagggt tgtgaacct tgcaattgt gacccgcaa ggctcacta cgaccacag tgcaacttg ttcgatggg caatgcgaa tgtcggcca ggttttggt ggcagacag tgtaacgaa tgtgtggcc agattctgg ggagacccc aaagttgaa tgtcgcgaa tgtaaatgt gacgcaaga gggtctagg tctttgcag tgtgacatg agaactggt gcttgtaag tgtttgcca gggataggt gggtataac tgcgacagg tgtgatcga ggctacttg ggacaagca cctgagtgt atcccttgt ggggaatgt ttcgataat tgggaccgc attttgcaa gaaacgcga aaccaaaca atggattta attaaccgt gccggtgat atcaagaaa gttggcgca actggcgct tacaccaaa gaattcgac gatatgcaa ttccaactt gacgaaata aaacaactg ttgagtaac acccacgaa gtcgatatt gacgccatt gataaggaa ttgcaagac ttacgtaca aaaatcaac gagactgag cttggaagc gtcaaagaa cttgatgat tcaatcgcc acaaccaaa gaaaatatg ctactaacc gacttaaaa ctgaaaagt ttgaaacaa cgaatcgac gactttaaa aacaaaaca aaagaactg gaaatgaac ggtacgcaa ctacaagaa gccaacgtc caaggggcc cttacccta atccaaaat gccaaagtc aaagctgat ctcgcagcg cataaagcg gaaagaacc gaagaaact ataaattat gccgaacgt cagattaaa gccactgaa agcacgatt aacgaaact gcacataac ttcaaccaa cagctgaag aacaacgaa aagaagctc atcgacttg aacgaaaaa cttgacagt cttaaacaa acccttccc gatttgaat gagcaaatt tgtgacggc agaggcgac ccttgtgac tcgttgtgt ggcggcgca ggttgtgcc acttgtggt gtgtcaatt tcgtgtgaa aatggggcc aaacaacag gctgaaacc gcgctagct atagcgaat cggacggaa atggccctc cgtgagaag gaagcaatg gctaatgat ttcatccga aatgtctct caaataaac acaaatgag acgaggaat atcgcacaa gagacgtac gataaagcc aagggaggg ttaatgaaa gctaatgac tcgttgaat aatgttttg aacttagtg aaggaagtt aacgagttt atggaccgc aatgacacc aaggaagat ataaaaaat ttcacgcag cagattttg gccatgaaa attgaactc gaaccggac gaaatcaca aacatggct aataaaatc aaagaagcg gtccaacac ttgactgat attgacccc attattgaa gctaccaaa gatgattta cggagagtt aacagactt agggaaaac gctgaatat gccaaaacg aatgcgtca aaaatattg accgatgcc gccgacgtg actagtact ttagaaaac acaacaatc gcgcaagat aatgcccaa agcgcaatt gacacagcc tggaatgac atcaacatt gtgaacgat cggttaaaa cagattggc tcgaaaaca cagtcggcc caaaaaaag acaaacaat acagccgac gacctcaaa aaactcgag gaacaactc aatcagctt caacgaaac ataaccgat aacggaatc tacgccaat cgtgtcgta aacgaatcg tcaaacatt ttggaaaaa gcccggaca acttacgac gacttcaac gaactccag gcacagtat tccaaagtt caacgcaat ttaacaacg agtttagac aaagttcag tcgtcgaaa gaccgcgct aatgatctt ttcaagcgt gcgtgggaa cttgttgcc aaagtaacg agtactgaa gaggaaatt agcaaactg gagaacagt tcccaacaa gcgacttta actaatttg gaaaatata ctacaagat ttgataagg cggatgaat ggctacaat caaaaaata caggagaga gtgcattac tacaaacag tgtaattaa
- >TC005184 RA
tcaagagag gacagacgg ccgacttcc cgactgggc ttcttgtct gtctccaac catgttccg cccaaattc ggcaatatg gacactttt agcctttat tttattcca agtagtaca aagtaattc acaagtatt cggtcaatc gtactcgcg gataagagg aaacccctc aggagggta cgtgccggt caagctaca tccttgtga gcaaagctc gtgctatcc agccactgg aaacctact aataggtcg ggaacatca gttgtacgc gtcttccac ttgcggcct ccacggccc acagagata ctgtattgt gtcgcattt ggaagagcg gaagaagtg ttttagatg cgattccag gccgaccaa caaaccaaa tcctttgtt taatcacaa cattagtaa tattgtcta taggatgta tcctgggac gcggcagaa gtcgtggtg gcagtccga aaacggcaa agagggtgt ctacatcca gttggatct agaagccga gtttcactt tacgcatct tattatcac gtttaagac ttttaggcc tgcggctat gttaatcga gcgttcgta cgatttcaa acagacgtg gcatgtcta tcgctactt tgcctacaa ctgcgctga aagttttcc gcatattag ggaaggata tcctgtgaa tctcactga tgttgtttg cgaatcgcg ctattcgtc gatagaccc gtcaacgga cggtgaagt gatttaccg cgtgttgcc cccaactct ccacattga caacccgta ctcggaaca agtccagaa cttgctcaa aatgaccaa tttgcggat caatttcac caaattaca cactctggg tgacgacct cctcgacaa acgcgagga catccaaga gaagtacta ctacgccat caccgacat ggtggtgcg cggctcctg ttcgtgcta cggacacgc taataggtg tctaccact gccggggat tgagcccaa gccggacat ggtgcacgg ccgctgcga gtgcacgca caacaccaa aggccgcaa ttgcgaaaa gtgtgagga ctttttcaa cgacttgcc gtggcggcc tgccatcgg caaacaaac caacgcgtg taaaaaatg caactgtaa caaccatgc aactagctg ccatttcga cgccgctct ctacgaagc caccggtcg catcagtgg tggcgtttg cgacgggtg ccagcacaa caccatggg ccccaactg cgagcagtg caaaccttt ctattacag ggacccgca gcgggacat ccaggaccc tgaagtgtg tcggccctg tgattgcga ccctcgcgg atcgcttga taacggcat ttgcgactc tatcaccga tgctgagaa taatctaat tgctggaag ttgccactg taagactaa tgtggaagg ccgccggtg cgatatttg caagaacgg cttttggaa ctttacgga aagtaatcc agatgggtg tcagagttg cacttgcaa cacttttgg aacaattga caatcaggg ttgtaatgt gtacaatgg ggagtgcac ttgtaagag gtatgtgac tgggcgcga ttgcaacca atgtctccc cgagtattg ggggctttc ggaaaaacg cgacggttg ccagccgtg cgattgtga tcctggagg ttcgttcga taacaattg cgacgttat cactggaca gtgtctctg ccgagaaca tatgacggg gcggacgtg tagcatccc caaacagca acactacac cgcaagttt ggacttcct attgtacga agctgagtc ggcgagggc gtcacctaa ctgtcaagt cgtaataag ggagccccc gcgggacgg gtccgatgt tacatggac cgggatcgg gtttatgaa ggcttttga gccgtcgta cattgaatt caccgtgga taacatcaa gacgggcat ggactacga aatcgtgat ccgctacga acccacaat gcccacaaa ctgggagga ggtcgaagt gaccctcga gcggccagg ccccgttga tccaaacgg cccttgcaa cggagcccg cgatgatcc cagaagagt cgcactacc gtcgaacag cagaagcgt cactgtgta cccccctgt gtgtctgga gcccggccg aacctacaa aatccggct gactttccg ccggtctaa cttcgaacg cgagacacc ctcagcttc ggtcttaat agactcggt cgtgttaat cccccgcat tgagaaaat cccctggtt ctacggctc ggtgcccgc cgaggcccg gcgccaaga attcgaacg caaccggtg cagctccta ctcctccta cctaatcca caccggaca aatccccga agtttgcaa aaagtacta cagcagcat tggcgctta catcttcaa cggcgcctt ctcctgtca atgcgaccc gaccggctc tctcagcaa gctttgcga cgagtatgg gggccactg cacctgcaa actgaacgt ggtcggccg ccggtgcga ccagtgcgc ccccgggac ttacggctt cgggccgga gggttgcaa gtcttgcga ttgcaacag tatcggggc ccttgacaa tttctgcaa tgccaccac tggacagtg caaatgccg ggccaacac gtacgggcg ggagtgcga ccagtgcag gaccggctt ttggaattt ccccaactg tcagaggtg cgactgtaa cgggcatgc ggatatttg tgattccaa aaccggggc ttgtatcaa ttgtaagga caacacgga ggggcacaa ttgcgagca atgcgtcga cgggtttta cggggatcc cagacttaa cgtggatat tccgtgccg gccgtgtcc ctgcccggg gagcccctg gtcgaatca ctcctacgc tgataggtg tagcctgga agcgagcac caaggatgt gatttgtga gtgtcatgt ggggtattc agggtcacg ttgtgacgt ctgttctga taactattt cggaaatcc ggaagaacc gggcggttt gtgccaacc gtgcgattg caacgataa aattgatat ccttaaacc gggaaattg tgacccaca caccggtaa atgcaagca atgtttgta tgatacgac gggagacca ctgcgaggt gtgtagacc cggcttttt ccgatattc cgatgacat gatgtgtga agagtgtgt ctgtcaccc attgggcac gaacagctc agccgggcc ttgtgaccc atcttccgg gcaatgcgt ctgtcttcc aaacgtgac cggattaaa ttgtgacca atgtatcgc caaccattg gaaaattgc aagtgggga gggttgtga accttgcaa ttgtgaccc gcaaggctc actacgacc acagtgcaa cttgttcga tgggcaatg cgaatgtcg gccaggttt tggtggcag acagtgtaa cgaatgtgt ggccagatt ctggggaga ccccaaagt tgaatgtcg cgaatgtaa atgtgacgc aagagggtc taggtcttt gcagtgtga catgagaac tggtgcttg taagtgttt gccagggat aggtgggta taactgcga caggtgtga tcgaggcta cttgggaca agcacctga gtgtatccc ttgtgggga atgtttcga taattggga ccgcatttt gcaagaaac gcgaaacca aacaatgga tttaattaa ccgtgccgg tgatatcaa gaaagttgg cgcaactgg cgcttacac caaagaatt cgacgatat gcaattcca acttgacga aataaaaca actgttgag taacaccca cgaagtcga tattgacgc cattgataa ggaattgca agacttacg tacaaaaat caacgagac tgagcttgg aagcgtcaa agaacttga tgattcaat cgccacaac caaagaaaa tatgctact aaccgactt aaaactgaa aagtttgaa acaacgaat cgacgactt taaaaacaa aacaaaaga actggaaat gaacggtac gcaactaca agaagccaa cgtccaagg ggcccttac cctaatcca aaatgccaa agtcaaagc tgatctcgc agcgcataa agcggaaag aaccgaaga aactataaa ttatgccga acgtcagat taaagccac tgaaagcac gattaacga aactgcaca taacttcaa ccaacagct gaagaacaa cgaaaagaa gctcatcga cttgaacga aaaacttga cagtcttaa acaaaccct tcccgattt gaatgagca aatttgtga cggcagagg cgacccttg tgactcgtt gtgtggcgg cgcaggttg tgccacttg tggtgtgtc aatttcgtg tgaaaatgg ggccaaaca acaggctga aaccgcgct agctatagc gaatcggac ggaaatggc cctccgtga gaaggaagc aatggctaa tgatttcat ccgaaatgt ctctcaaat aaacacaaa tgagacgag gaatatcgc acaagagac gtacgataa agccaaggg agggttaat gaaagctaa tgactcgtt gaataatgt tttgaactt agtgaagga agttaacga gtttatgga ccgcaatga caccaagga agatataaa aaatttcac gcagcagat tttggccat gaaaattga actcgaacc ggacgaaat cacaaacat ggctaataa aatcaaaga agcggtcca acacttgac tgatattga ccccattat tgaagctac caaagatga tttacggag agttaacag acttaggga aaacgctga atatgccaa aacgaatgc gtcaaaaat attgaccga tgccgccga cgtgactag tactttaga aaacacaac aatcgcgca agataatgc ccaaagcgc aattgacac agcctggaa tgacatcaa cattgtgaa cgatcggtt aaaacagat tggctcgaa aacacagtc ggcccaaaa aaagacaaa caatacagc cgacgacct caaaaaact cgaggaaca actcaatca gcttcaacg aaacataac cgataacgg aatctacgc caatcgtgt cgtaaacga atcgtcaaa cattttgga aaaagcccg gacaactta cgacgactt caacgaact ccaggcaca gtattccaa agttcaacg caatttaac aacgagttt agacaaagt tcagtcgtc gaaagaccg cgctaatga tcttttcaa gcgtgcgtg ggaacttgt tgccaaagt aacgagtac tgaagagga aattagcaa actggagaa cagttccca acaagcgac tttaactaa tttggaaaa tatactaca agatttgat aaggcggat gaatggcta caatcaaaa aatacagga gagagtgca ttactacaa acagtgtaa ttaacagca tgtaaatat gtagctttc actgccttt ttatcgtag cttaggaat aagagttta ttttttact tgttataac caatagtgc caacgagct tgtacatat acttttagc gttttatat tcgaacttt tatctgtaa tcaactact aattttaat aaaacttta taatattg
- >TC005184 RB
tcaagagag gacagacgg ccgacttcc cgactgggc ttcttgtct gtctccaac catgttccg cccaaattc ggcaatatg gacactttt agcctttat tttattcca agtagtaca aagtaattc acaagtatt cggtcaatc gtactcgcg gataagagg aaacccctc aggagcttc agtaaaaag gggtgacca accgcctga tatagatag cgagtaccc cgacggcga cgttcttca tcccttgac ggatgtctt caatcgccg accttacac tcataatca tgggtacgt gccggtcaa gctacatcc ttgtgagca aagctcgtg ctatccagc cactggaaa cctactaat aggtcggga acatcagtt gtacgcgtc ttccacttg cggcctcca cggcccaca gagatactg tattgtgtc gcatttgga agagcggaa gaagtgttt tagatgcga ttccaggcc gaccaacaa accaaatcc tttgtttaa tcacaacat tagtaatat tgtctatag gatgtatcc tgggacgcg gcagaagtc gtggtggca gtccgaaaa cggcaaaga gggtgtcta catccagtt ggatctaga agccgagtt tcactttac gcatcttat tatcacgtt taagacttt taggcctgc ggctatgtt aatcgagcg ttcgtacga tttcaaaca gacgtggca tgtctatcg ctactttgc ctacaactg cgctgaaag ttttccgca tattaggga aggatatcc tgtgaatct cactgatgt tgtttgcga atcgcgcta ttcgtcgat agacccgtc aacggacgg tgaagtgat ttaccgcgt gttgccccc aactctcca cattgacaa cccgtactc ggaacaagt ccagaactt gctcaaaat gaccaattt gcggatcaa tttcaccaa attacacac tctgggtga cgacctcct cgacaaacg cgaggacat ccaagagaa gtactacta cgccatcac cgacatggt ggtgcgcgg ctcctgttc gtgctacgg acacgctaa taggtgtct accactgcc ggggattga gcccaagcc ggacatggt gcacggccg ctgcgagtg cacgcacaa caccaaagg ccgcaattg cgaaaagtg tgaggactt tttcaacga cttgccgtg gcggcctgc catcggcaa acaaaccaa cgcgtgtaa aaaatgcaa ctgtaacaa ccatgcaac tagctgcca tttcgacgc cgctctcta cgaagccac cggtcgcat cagtggtgg cgtttgcga cgggtgcca gcacaacac catgggccc caactgcga gcagtgcaa acctttcta ttacaggga cccgcagcg ggacatcca ggaccctga agtgtgtcg gccctgtga ttgcgaccc tcgcggatc gcttgataa cggcatttg cgactctat caccgatgc tgagaataa tctaattgc tggaagttg ccactgtaa gactaatgt ggaaggccg ccggtgcga tatttgcaa gaacggctt ttggaactt tacggaaag taatccaga tgggtgtca gagttgcac ttgcaacac ttttggaac aattgacaa tcagggttg taatgtgta caatgggga gtgcacttg taagaggta tgtgactgg gcgcgattg caaccaatg tctccccga gtattgggg gctttcgga aaaacgcga cggttgcca gccgtgcga ttgtgatcc tggaggttc gttcgataa caattgcga cgttatcac tggacagtg tctctgccg agaacatat gacggggcg gacgtgtag catccccaa acagcaaca ctacaccgc aagtttgga cttcctatt gtacgaagc tgagtcggc gagggcgtc acctaactg tcaagtcgt aataaggga gcccccgcg ggacgggtc cgatgttac atggaccgg gatcgggtt tatgaaggc ttttgagcc gtcgtacat tgaattcac cgtggataa catcaagac gggcatgga ctacgaaat cgtgatccg ctacgaacc cacaatgcc cacaaactg ggaggaggt cgaagtgac cctcgagcg gccaggccc cgttgatcc aaacggccc ttgcaacgg agcccgcga tgatcccag aagagtcgc actaccgtc gaacagcag aagcgtcac tgtgtaccc ccctgtgtg tctggagcc cggccgaac ctacaaaat ccggctgac tttccgccg gtctaactt cgaacgcga gacaccctc agcttcggt cttaataga ctcggtcgt gttaatccc ccgcattga gaaaatccc ctggttcta cggctcggt gcccgccga ggcccggcg ccaagaatt cgaacgcaa ccggtgcag ctcctactc ctcctacct aatccacac cggacaaat ccccgaagt ttgcaaaaa gtactacag cagcattgg cgcttacat cttcaacgg cgccttctc ctgtcaatg cgacccgac cggctctct cagcaagct ttgcgacga gtatggggg ccactgcac ctgcaaact gaacgtggt cggccgccg gtgcgacca gtgcgcccc cgggactta cggcttcgg gccggaggg ttgcaagtc ttgcgattg caacagtat cggggccct tgacaattt ctgcaatgc caccactgg acagtgcaa atgccgggc caacacgta cgggcggga gtgcgacca gtgcaggac cggcttttg gaatttccc caactgtca gaggtgcga ctgtaacgg gcatgcgga tatttgtga ttccaaaac cggggcttg tatcaattg taaggacaa cacggaggg gcacaattg cgagcaatg cgtcgacgg gttttacgg ggatcccag acttaacgt ggatattcc gtgccggcc gtgtccctg cccggggag cccctggtc gaatcactc ctacgctga taggtgtag cctggaagc gagcaccaa ggatgtgat ttgtgagtg tcatgtggg gtattcagg gtcacgttg tgacgtctg ttctgataa ctatttcgg aaatccgga agaaccggg cggtttgtg ccaaccgtg cgattgcaa cgataaaat tgatatcct taaaccggg aaattgtga cccacacac cggtaaatg caagcaatg tttgtatga tacgacggg agaccactg cgaggtgtg tagacccgg ctttttccg atattccga tgacatgat gtgtgaaga gtgtgtctg tcacccatt gggcacgaa cagctcagc cgggccttg tgacccatc ttccgggca atgcgtctg tcttccaaa cgtgaccgg attaaattg tgaccaatg tatcgccaa ccattggaa aattgcaag tggggaggg ttgtgaacc ttgcaattg tgacccgca aggctcact acgaccaca gtgcaactt gttcgatgg gcaatgcga atgtcggcc aggttttgg tggcagaca gtgtaacga atgtgtggc cagattctg gggagaccc caaagttga atgtcgcga atgtaaatg tgacgcaag agggtctag gtctttgca gtgtgacat gagaactgg tgcttgtaa gtgtttgcc agggatagg tgggtataa ctgcgacag gtgtgatcg aggctactt gggacaagc acctgagtg tatcccttg tggggaatg tttcgataa ttgggaccg cattttgca agaaacgcg aaaccaaac aatggattt aattaaccg tgccggtga tatcaagaa agttggcgc aactggcgc ttacaccaa agaattcga cgatatgca attccaact tgacgaaat aaaacaact gttgagtaa cacccacga agtcgatat tgacgccat tgataagga attgcaaga cttacgtac aaaaatcaa cgagactga gcttggaag cgtcaaaga acttgatga ttcaatcgc cacaaccaa agaaaatat gctactaac cgacttaaa actgaaaag tttgaaaca acgaatcga cgactttaa aaacaaaac aaaagaact ggaaatgaa cggtacgca actacaaga agccaacgt ccaaggggc ccttaccct aatccaaaa tgccaaagt caaagctga tctcgcagc gcataaagc ggaaagaac cgaagaaac tataaatta tgccgaacg tcagattaa agccactga aagcacgat taacgaaac tgcacataa cttcaacca acagctgaa gaacaacga aaagaagct catcgactt gaacgaaaa acttgacag tcttaaaca aacccttcc cgatttgaa tgagcaaat ttgtgacgg cagaggcga cccttgtga ctcgttgtg tggcggcgc aggttgtgc cacttgtgg tgtgtcaat ttcgtgtga aaatggggc caaacaaca ggctgaaac cgcgctagc tatagcgaa tcggacgga aatggccct ccgtgagaa ggaagcaat ggctaatga tttcatccg aaatgtctc tcaaataaa cacaaatga gacgaggaa tatcgcaca agagacgta cgataaagc caagggagg gttaatgaa agctaatga ctcgttgaa taatgtttt gaacttagt gaaggaagt taacgagtt tatggaccg caatgacac caaggaaga tataaaaaa tttcacgca gcagatttt ggccatgaa aattgaact cgaaccgga cgaaatcac aaacatggc taataaaat caaagaagc ggtccaaca cttgactga tattgaccc cattattga agctaccaa agatgattt acggagagt taacagact tagggaaaa cgctgaata tgccaaaac gaatgcgtc aaaaatatt gaccgatgc cgccgacgt gactagtac tttagaaaa cacaacaat cgcgcaaga taatgccca aagcgcaat tgacacagc ctggaatga catcaacat tgtgaacga tcggttaaa acagattgg ctcgaaaac acagtcggc ccaaaaaaa gacaaacaa tacagccga cgacctcaa aaaactcga ggaacaact caatcagct tcaacgaaa cataaccga taacggaat ctacgccaa tcgtgtcgt aaacgaatc gtcaaacat tttggaaaa agcccggac aacttacga cgacttcaa cgaactcca ggcacagta ttccaaagt tcaacgcaa tttaacaac gagtttaga caaagttca gtcgtcgaa agaccgcgc taatgatct tttcaagcg tgcgtggga acttgttgc caaagtaac gagtactga agaggaaat tagcaaact ggagaacag ttcccaaca agcgacttt aactaattt ggaaaatat actacaaga tttgataag gcggatgaa tggctacaa tcaaaaaat acaggagag agtgcatta ctacaaaca gtgtaatta acagcatgt aaatatgta gctttcact gccttttta tcgtagctt aggaataag agtttattt tttacttgt tataaccaa tagtgccaa cgagcttgt acatatact tttagcgtt ttatattcg aacttttat ctgtaatca actactaat tttaataaa actttataa tattg
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