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Gene information
General information
TC030592 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3062015..3063955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030592
    iBeetle screen iB_08657

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030592 RA
      MDENKTRDL DDVNEMLKL AKLGENDVL STSQTVYFT 
      SDNLNCDNF KFLELDQHL LEEIKQGQT LYIKGDDDE 
      NVVLCTKDR TYDVTGAET SNSLLLVKN MAFFDDLKD 
      GKERALQTV TVSGVFYDY LSVTPGKPH LKKLRDLLD 
      KSVYKGPEH EYEIKNEDL YGYDDLIKI IQASDQELE 
      SVLKQMNVV TINGKIRLL DVEYHFRAL SYMLKLIEE 
      NSWNLDEID FDETLNSLR DIIPETILK NLFDKYTTE 
      SKIVDGLQL YSYKEREIC RFFAQILLY GAGKFNLEE 
      FLQAWRESV PEGMTCDEE LLHGIAIID RKSDPPVIS 
      AFPEEELPE EINARFNKL FKAKEKWTV PEITPYIQR 
      VATDKMDVN ALLAKFARA SNSQGVKFY SAKHAK
    CDS sequence
    • >TC030592 RA
      atggacgaa aataaaacg cgggatttg gacgatgtg 
      aatgaaatg cttaaattg gcgaaactg ggcgagaac 
      gacgtgtta tcaacctca caaacggtt tatttcact 
      agtgataat ctcaactgt gacaatttt aaatttctc 
      gagcttgac cagcaccta cttgaggaa ataaaacag 
      ggacagaca ctctatatt aaaggagac gacgacgaa 
      aacgtcgta ctttgtaca aaagaccgc acgtatgat 
      gttacaggg gccgaaacg tccaatagt ttattgctg 
      gtgaaaaat atggccttt ttcgatgat ttgaaagat 
      gggaaagaa agggcgctc caaacggtc accgtttcg 
      ggggttttt tacgattat ttgtccgta actcccggc 
      aagcctcat ttaaaaaaa ctgcgagat ttgttggac 
      aaatccgtg tataaaggg cccgaacac gagtatgaa 
      ataaaaaat gaggatctg tacggatac gacgacttg 
      attaaaatt attcaagcg tcagatcaa gagctcgaa 
      agcgtcctc aaacaaatg aacgtcgtg acaattaac 
      gggaaaatc aggctactt gatgtggaa tatcatttt 
      cgcgccttg tcttatatg ttaaagcta attgaggaa 
      aactcgtgg aatttagac gagatcgat tttgacgaa 
      acgctaaac tcgctcagg gatataatc ccagaaaca 
      atccttaaa aatttattc gacaagtat acaacagag 
      tctaaaatt gtcgacggt ttgcaattg tattcctac 
      aaagaacgt gaaatttgc cggtttttc gcccaaatc 
      ttgctctac ggggcggga aaatttaat ttggaggag 
      tttttgcaa gcttggagg gagtcagtt cccgaggga 
      atgacctgt gatgaggaa ctcctacac ggaattgcg 
      ataattgac cggaagagt gaccctccg gttatttcc 
      gccttccca gaagaggaa ttacccgaa gaaatcaac 
      gccaggttc aataaatta tttaaagct aaggaaaaa 
      tggacagtg cctgaaata actccttac atccagcgc 
      gtcgcgacg gacaaaatg gacgtcaat gccttgctt 
      gcgaaattc gcacgggcg tccaattcg caaggagtc 
      aagttttac tcggccaag catgccaag taa
    mRNA sequence
    • >TC030592 RA
      ttcacgcaa ccatggacg aaaataaaa cgcgggatt 
      tggacgatg tgaatgaaa tgcttaaat tggcgaaac 
      tgggcgaga acgacgtgt tatcaacct cacaaacgg 
      tttatttca ctagtgata atctcaact gtgacaatt 
      ttaaatttc tcgagcttg accagcacc tacttgagg 
      aaataaaac agggacaga cactctata ttaaaggag 
      acgacgacg aaaacgtcg tactttgta caaaagacc 
      gcacgtatg atgttacag gggccgaaa cgtccaata 
      gtttattgc tggtgaaaa atatggcct ttttcgatg 
      atttgaaag atgggaaag aaagggcgc tccaaacgg 
      tcaccgttt cgggggttt tttacgatt atttgtccg 
      taactcccg gcaagcctc atttaaaaa aactgcgag 
      atttgttgg acaaatccg tgtataaag ggcccgaac 
      acgagtatg aaataaaaa atgaggatc tgtacggat 
      acgacgact tgattaaaa ttattcaag cgtcagatc 
      aagagctcg aaagcgtcc tcaaacaaa tgaacgtcg 
      tgacaatta acgggaaaa tcaggctac ttgatgtgg 
      aatatcatt ttcgcgcct tgtcttata tgttaaagc 
      taattgagg aaaactcgt ggaatttag acgagatcg 
      attttgacg aaacgctaa actcgctca gggatataa 
      tcccagaaa caatcctta aaaatttat tcgacaagt 
      atacaacag agtctaaaa ttgtcgacg gtttgcaat 
      tgtattcct acaaagaac gtgaaattt gccggtttt 
      tcgcccaaa tcttgctct acggggcgg gaaaattta 
      atttggagg agtttttgc aagcttgga gggagtcag 
      ttcccgagg gaatgacct gtgatgagg aactcctac 
      acggaattg cgataattg accggaaga gtgaccctc 
      cggttattt ccgccttcc cagaagagg aattacccg 
      aagaaatca acgccaggt tcaataaat tatttaaag 
      ctaaggaaa aatggacag tgcctgaaa taactcctt 
      acatccagc gcgtcgcga cggacaaaa tggacgtca 
      atgccttgc ttgcgaaat tcgcacggg cgtccaatt 
      cgcaaggag tcaagtttt actcggcca agcatgcca 
      agtaaactc accattaat tgtgtgaat gccgcttta 
      gctacatgt tgcgcgcgc ttcttgtaa ataataata 
      ataaatttg gccaaaaat gcccgtctg tagcgaaaa 
      ctacgagag cgagattgc agaagggcg taattgtcg 
      aagtgtgaa atgcaagtt ttgcggttc ggtgatttt 
      gagtccttc gactgcgac atttgccag ttttgaggt 
      taggtgtga tgattggtg gttccgtgc tgattttcc 
      cccagtttc agttgcccc tcatccgcc agaataaat 
      cgaatgaga ctgagcccg aaactgaga ccgtttcgt 
      ttttctgga gcgttagcg acatgtaca ctttccaaa 
      atgtggggt tttcgcaca ctgttggca acaataagt 
      atctggcgt gtgccgact gcgaggctg ggcctattg 
      gctatcacg atctgacga ataagacca gcttcgtgg 
      cgctgagtc gcgtccaac attcttaaa ctttatatt 
      ttgtagttt tttaatttc gtgaaataa ataatttaa 
      attctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY