Status
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Gene information
General information
TC009595 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3064047..3065662
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009595
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08656

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellarium size decreased - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    6

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009595 RA
      MTSPLKPWE SNSLQNASQ IRSSHNLRD VPMAGRSAP 
      VLPPLPRTS PMVASSAYS SYMPYSSGY SSLGYGMPY 
      RSSLYNSYG SYGGYNSYN MYGMGGYPS FGVNDDAER 
      RFIQYAEES SRNTFASVE SIVRAVNSI SMMLDNTFF 
      AMTSSFRAV LSVADNFGR LRSMFGHIW YSINIFRLF 
      NWLYRKFMW LIGRKVPNS ATSLAWRQA SGAPPTGPA 
      PGSSWPTLA FLGVLVSAP YIISKFLPK YEDKCDPAN 
      WKSPGVRAK AAFDFVARS QNEMSVQTN DELTLAPTY 
      VQEEMNLRN TGWAFAVAN GRSGVVPLN YLVISKAGP 
      REGTEEVPV PRVFNKTHT KRVSFGENQ VFENVDLDD 
      YVTGKKEIK GVDDIKNSV KEGVDDNKY SLKEVSDSK 
      KDEQQNKGE NEITNQVET KKVESN
    CDS sequence
    • >TC009595 RA
      atgacatcg ccgctgaaa ccttgggag tccaactct 
      ttgcaaaac gcgtctcaa attcgctcc tcgcacaat 
      ttgcgcgat gttccaatg gctgggcgc agcgccccc 
      gtgttgccc cctttgcct cggacgtcg cccatggtg 
      gcctcgtcc gcctacagc tcttacatg ccttattct 
      agtggttat agcagtctg gggtacggc atgccgtac 
      cgcagctcc ctgtacaat tcctacggc tcgtatggg 
      ggctacaac agctacaac atgtacggc atggggggt 
      taccccagt tttggggta aacgacgac gccgagcgt 
      cgctttatc caatacgcg gaggaaagt tcacgcaac 
      actttcgca agtgtggaa agtatagtc cgtgccgtt 
      aacagcatt tctatgatg cttgacaac acatttttc 
      gctatgacg agttcgttc agggccgta ctcagcgtg 
      gccgacaac ttcgggcgc ctccgctcc atgttcggc 
      cacatctgg tactccata aacattttc cgcttgttt 
      aattggctg taccgcaaa ttcatgtgg ctgattggc 
      cgcaaggtg cccaatagc gccacttct ctggcgtgg 
      aggcaggcc agtggggcg ccgcccact gggcccgcc 
      cccggctcc agttggcca actcttgcg tttctgggc 
      gttttggtc tcggcgccg tacattatc agcaaattc 
      ctccccaaa tatgaagat aagtgtgat ccagccaat 
      tggaaaagt ccaggcgtt agggcgaag gcggcgttt 
      gatttcgtg gcccggagt cagaatgag atgagcgtt 
      cagacgaat gatgaactg actttggcg cccacttat 
      gtgcaggag gaaatgaat ttgaggaat acggggtgg 
      gcgtttgct gtggccaat ggccggtcg ggggtggtg 
      ccgttgaat tacctcgtg attagcaag gcggggccg 
      agggagggg acagaggag gtgccagta cctagggtt 
      tttaataaa acgcacacg aaacgtgtc agttttggg 
      gagaatcaa gtttttgaa aacgttgat ttggacgat 
      tatgttacg gggaaaaag gaaataaag ggggtagat 
      gatattaaa aactctgtt aaagaaggg gtagatgat 
      aataaatat tctctcaaa gaggtaagt gatagtaaa 
      aaagacgaa cagcaaaat aaaggcgaa aatgaaata 
      accaatcaa gttgaaacc aaaaaagtc gagtcaaat 
      taa
    mRNA sequence
    • >TC009595 RA
      taagataag aaaatcgca gattgtttt gttaaatcc 
      ttacaatta cttgcaatt gtgcgaaaa atgtgattg 
      ggggtttgg gacaatgac atcgccgct gaaaccttg 
      ggagtccaa ctctttgca aaacgcgtc tcaaattcg 
      ctcctcgca caatttgcg cgatgttcc aatggctgg 
      gcgcagcgc ccccgtgtt gcccccttt gcctcggac 
      gtcgcccat ggtggcctc gtccgccta cagctctta 
      catgcctta ttctagtgg ttatagcag tctggggta 
      cggcatgcc gtaccgcag ctccctgta caattccta 
      cggctcgta tgggggcta caacagcta caacatgta 
      cggcatggg gggttaccc cagttttgg ggtaaacga 
      cgacgccga gcgtcgctt tatccaata cgcggagga 
      aagttcacg caacacttt cgcaagtgt ggaaagtat 
      agtccgtgc cgttaacag catttctat gatgcttga 
      caacacatt tttcgctat gacgagttc gttcagggc 
      cgtactcag cgtggccga caacttcgg gcgcctccg 
      ctccatgtt cggccacat ctggtactc cataaacat 
      tttccgctt gtttaattg gctgtaccg caaattcat 
      gtggctgat tggccgcaa ggtgcccaa tagcgccac 
      ttctctggc gtggaggca ggccagtgg ggcgccgcc 
      cactgggcc cgcccccgg ctccagttg gccaactct 
      tgcgtttct gggcgtttt ggtctcggc gccgtacat 
      tatcagcaa attcctccc caaatatga agataagtg 
      tgatccagc caattggaa aagtccagg cgttagggc 
      gaaggcggc gtttgattt cgtggcccg gagtcagaa 
      tgagatgag cgttcagac gaatgatga actgacttt 
      ggcgcccac ttatgtgca ggaggaaat gaatttgag 
      gaatacggg gtgggcgtt tgctgtggc caatggccg 
      gtcgggggt ggtgccgtt gaattacct cgtgattag 
      caaggcggg gccgaggga ggggacaga ggaggtgcc 
      agtacctag ggtttttaa taaaacgca cacgaaacg 
      tgtcagttt tggggagaa tcaagtttt tgaaaacgt 
      tgatttgga cgattatgt tacggggaa aaaggaaat 
      aaagggggt agatgatat taaaaactc tgttaaaga 
      aggggtaga tgataataa atattctct caaagaggt 
      aagtgatag taaaaaaga cgaacagca aaataaagg 
      cgaaaatga aataaccaa tcaagttga aaccaaaaa 
      agtcgagtc aaattaagt tttattgca tttgttgtg 
      tagttgttt tgtcactca attagctaa ggtgagaga 
      actgatttt atatgtact atttcaatt ttttacgat 
      ctagctagg tgatatgtg aactactta ggttatgtg 
      ttatttttt attggagtt tgcttcaaa taaagtttt 
      attgctcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY