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Gene information
General information
TC009210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3087481..3089175
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009210
    iBeetle screen iB_08654

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 3
    yolk late structure irregular - Number of animals showing the phenotype: 1

    mature egg not present - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009210 RA
      MEEPKREFP KPKRSKTDH VKNIVHSYL QKRNYAPIG 
      IENCRPDQQ LTYSLIENE ISRPNSILY SCYNSDPVV 
      IDHNFTKFI TWLKDCSKT KNCPDLEQL TAPFFCHLY 
      LDILKGGHS DRAATFLKA HLPAIDKTK CDDFVKDLV 
      NLFANDCDA TCMIEQFRN NKYYVELLP QSLALLKKF 
      VAENCHVVF LHVLQTWFD IRQSEDRDN ESEGKMDDK 
      GAHNGVCLD DKLRKILDT INDLDKEPK QIFLANISN 
      TNEPISSAL IKRTCGLIA YNQHNIVHL RPVHSYDPI 
      LGVRDCKEI KLNDHTKRI YSINISPDC CKLVTGSED 
      SNLCIYDLN EIKLIKNCT GHLGPIYCT TISSNSVYA 
      ASGSLDTTA RLWDLEKGH TLRVFVGHT EAITCIDFH 
      PNCLYVATG SADRNIRLW SVDNGNPLR LFHGCKGVV 
      YGVLFSPLG DVLASVSDD KKVRLWDIL NSKVNSEIK 
      QKGLPATKL VWSSDGKQL CFRQLE
    CDS sequence
    • >TC009210 RA
      atggaagag ccaaaacgc gagttcccc aagcccaaa 
      cgttcaaaa accgaccac gtgaagaac atcgtgcac 
      tcctatctc cagaaacgc aactacgcc ccgattggc 
      attgaaaac tgccgtccc gaccagcaa ctcacttac 
      tccctcatc gaaaacgaa atcagtcgc cccaactca 
      atcctctac agttgttac aacagcgac ccggtcgta 
      atcgaccac aacttcacc aaattcatc acctggctc 
      aaagactgc tcaaaaacg aaaaactgt cccgatttg 
      gaacaactc acagccccc tttttctgt catttgtac 
      cttgacatt ctcaaaggg gggcactcg gaccgcgct 
      gccacgttc ctcaaagcg caccttccg gccatcgat 
      aagacgaaa tgtgacgat tttgttaaa gacttggtg 
      aatttattc gcaaatgat tgtgacgcc acttgcatg 
      attgaacag ttccgtaac aacaaatat tacgtcgag 
      ttattgcca caatcgctc gccttattg aagaaattc 
      gtggcggaa aactgccac gttgttttt ttacacgtg 
      ctacaaacg tggttcgat atcagacaa agtgaggat 
      agagataat gaaagtgag gggaaaatg gacgacaag 
      ggggcgcac aatggcgtg tgtctcgac gacaagtta 
      cgcaaaatt ttggacacg atcaacgac ctggacaag 
      gaaccgaaa caaattttc ttggccaac atttcaaac 
      accaatgag ccgatttca agcgctttg attaaacgc 
      acttgtggt ttaatcgcc tacaatcaa cacaacatc 
      gttcatctg cgccccgtt cattcgtac gaccccatt 
      ctgggggta cgtgactgc aaggaaatc aaattaaac 
      gatcacacg aaacgcatt tattcaata aatatttcg 
      cccgattgt tgcaaactt gtgacaggt tccgaagac 
      tcaaacttg tgcatttac gacttgaac gaaatcaaa 
      ttaattaaa aattgtacg ggtcatttg ggccccatt 
      tactgcacc actattagt agtaatagt gtttacgca 
      gcgtcgggt tcactggac acaacagcc cggctttgg 
      gaccttgaa aaggggcac acgttacgt gtctttgtg 
      gggcacact gaagccatc acgtgtatt gacttccat 
      ccgaattgt ttgtacgtg gcgaccggt tcagctgat 
      cgtaatatc cgactttgg agtgttgat aatgggaat 
      ccgttaaga ctttttcac ggttgtaaa ggtgtcgtt 
      tatggggtt ttatttagc ccacttggt gatgttttg 
      gctagtgtt agtgacgat aaaaaagtg agactttgg 
      gatatttta aattcgaag gttaatagt gaaattaag 
      caaaagggg ctgccggcc actaaacta gtgtggagt 
      agtgatggg aaacagttg tgttttaga cagctcgag 
      tga
    mRNA sequence
    • >TC009210 RA
      atgacccaa aaaaccatc attgttgtg tttttataa 
      ttgtttgtg gccgtttat tacggaaaa atctcgctt 
      ttttgctga tttcgcata aaaacgtcg cgtgctgtc 
      aagcgcgcc ctaacctca ctaccccag ttcgtgatg 
      gaagagcca aaacgcgag ttccccaag cccaaacgt 
      tcaaaaacc gaccacgtg aagaacatc gtgcactcc 
      tatctccag aaacgcaac tacgccccg attggcatt 
      gaaaactgc cgtcccgac cagcaactc acttactcc 
      ctcatcgaa aacgaaatc agtcgcccc aactcaatc 
      ctctacagt tgttacaac agcgacccg gtcgtaatc 
      gaccacaac ttcaccaaa ttcatcacc tggctcaaa 
      gactgctca aaaacgaaa aactgtccc gatttggaa 
      caactcaca gcccccttt ttctgtcat ttgtacctt 
      gacattctc aaagggggg cactcggac cgcgctgcc 
      acgttcctc aaagcgcac cttccggcc atcgataag 
      acgaaatgt gacgatttt gttaaagac ttggtgaat 
      ttattcgca aatgattgt gacgccact tgcatgatt 
      gaacagttc cgtaacaac aaatattac gtcgagtta 
      ttgccacaa tcgctcgcc ttattgaag aaattcgtg 
      gcggaaaac tgccacgtt gttttttta cacgtgcta 
      caaacgtgg ttcgatatc agacaaagt gaggataga 
      gataatgaa agtgagggg aaaatggac gacaagggg 
      gcgcacaat ggcgtgtgt ctcgacgac aagttacgc 
      aaaattttg gacacgatc aacgacctg gacaaggaa 
      ccgaaacaa attttcttg gccaacatt tcaaacacc 
      aatgagccg atttcaagc gctttgatt aaacgcact 
      tgtggttta atcgcctac aatcaacac aacatcgtt 
      catctgcgc cccgttcat tcgtacgac cccattctg 
      ggggtacgt gactgcaag gaaatcaaa ttaaacgat 
      cacacgaaa cgcatttat tcaataaat atttcgccc 
      gattgttgc aaacttgtg acaggttcc gaagactca 
      aacttgtgc atttacgac ttgaacgaa atcaaatta 
      attaaaaat tgtacgggt catttgggc cccatttac 
      tgcaccact attagtagt aatagtgtt tacgcagcg 
      tcgggttca ctggacaca acagcccgg ctttgggac 
      cttgaaaag gggcacacg ttacgtgtc tttgtgggg 
      cacactgaa gccatcacg tgtattgac ttccatccg 
      aattgtttg tacgtggcg accggttca gctgatcgt 
      aatatccga ctttggagt gttgataat gggaatccg 
      ttaagactt tttcacggt tgtaaaggt gtcgtttat 
      ggggtttta tttagccca cttggtgat gttttggct 
      agtgttagt gacgataaa aaagtgaga ctttgggat 
      attttaaat tcgaaggtt aatagtgaa attaagcaa 
      aaggggctg ccggccact aaactagtg tggagtagt 
      gatgggaaa cagttgtgt tttagacag ctcgagtga 
      acctgtgat tagtttaaa cgtgaatgg a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY