Status
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Gene information
General information
TC009234 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2835421..2840440
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC009234
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08647

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009234 RA
      MASFAAQAA LRAKWSSLV EEHSIEVPP EVTSKVTSV 
VGWLKQASL EVRAAGRRA VNTLAGGMA HHLVILHFN 
DVYNVEPRQ GPEPVGGAA RFCTAIKSF QHLHPLVLF 
SGDAFSPSM LSTYTKGEQ MVPVLNDLG THCAVLGNH 
DFDHGLEVL SQWVAQTEF PWLMSNVLD NETGRPLGE 
GRITHVVHW AGHRIGLLG LVEKEWLDT LATINPEMV 
TFLDFVEAG QKLAAQLKQ EGCDYVIAL THMRTPNDI 
KLAENCDNI DLILGGHDH VYEIKEING KFVVKSGTD 
FRQFSKITV NFDKPNGTP EVTIEEINV TSAFPEDPK 
LKEKLDKYT SIVEGKMHE VLGCFSVPL DGRFTSIRT 
SESNLGNWV CDVVLAATG ADLVILNSG TFRSDQVHP 
AGDFTVRDL TNIVPMRDP LVILKLTGK QILEALENG 
VCMYPKLEG RFPQVAGVS FAFDPSKAP GQRVDPAFV 
RIGDEYLNL EQNYRLATK SYMHSGCDG YVMLKDAEV 
LVDEGECPE LGLAIQNHF QAINMRLGK TKKHSKHRQ 
SLVTLSRRH SLVKMLDGS ELDGPPPLR RASTVEVTT 
SPPSHHTAR LTRRASLDD LEQESCQLT PKIDHRIVV 
ITNEEKRQE LILQRQRIE QDSIIEEVD EYSPQN
    CDS sequence
    • >TC009234 RA
      atggccagt ttcgcggcg caggccgcc ctccgtgcg 
aaatggagc tcgctggtg gaggagcac agcatcgag 
gtgcccccc gaagtcacc agcaaagtg acaagtgtg 
gtgggctgg ctcaaacag gcttcgctg gaggttcga 
gcggccggt cgaagggct gtcaatacg ttggccggg 
ggcatggcg caccatttg gtcattctg cacttcaac 
gatgtgtac aatgttgag ccgagacag gggccggag 
cccgtgggg ggcgcagcc cggttttgc acggcgatt 
aagagtttc cagcacttg catcctctg gtgctgttc 
agcggggac gctttctcg cccagcatg ttgagcacc 
tacacgaaa ggtgagcaa atggtcccc gtcctcaac 
gacctcggc acccactgt gccgtcctc ggcaaccac 
gatttcgat cacggcctt gaagtgctc tcccaatgg 
gtggcccaa accgaattc ccttggctc atgtccaac 
gtactcgat aacgaaacc ggccgtcca ttgggcgaa 
gggcggatc acccatgtg gtacattgg gcaggccac 
agaatcggt cttttgggc cttgttgaa aaagaatgg 
ctggacaca cttgcaacc atcaaccca gaaatggtc 
acttttctc gatttcgtc gaagcggga caaaaactc 
gccgcgcaa ttgaaacaa gaggggtgc gattatgtg 
atcgctctg actcatatg cgaacgcct aacgatatc 
aagctggcg gaaaattgc gataacatc gatttgata 
cttgggggg catgaccac gtgtacgaa atcaaggaa 
atcaacggg aaatttgtg gtgaaaagt ggcaccgac 
tttcggcag tttagtaaa attacggtg aattttgat 
aaaccgaat ggtactcct gaagtaaca attgaggaa 
attaacgtt acttctgcc tttcctgag gatccaaaa 
ttgaaggaa aaactggat aaatacacg agtattgtg 
gaggggaaa atgcacgaa gttttgggt tgtttttcc 
gtgccactt gatggacgt ttcacctcg attcgcact 
tcggaatcg aacttagga aattgggtc tgcgatgtg 
gttttggca gcaacaggt gcggattta gtaatttta 
aattcgggc acttttcgc tcggatcag gtgcaccca 
gctggggac ttcacagtt cgcgatttg accaacatt 
gtgcccatg agagacccc ttggtcatc ctcaaatta 
acaggcaaa caaattttg gaagcgctg gaaaatggg 
gtgtgtatg tacccgaaa ttggagggg aggttcccg 
caagtggcg ggggtgagt tttgccttc gacccctcc 
aaggcacca ggacaacga gtcgaccct gcctttgtc 
cgaatcgga gacgagtat ttgaatttg gagcagaat 
tatcggctt gcgaccaag agttacatg cattcaggg 
tgtgatggt tacgttatg cttaaagat gccgaagtt 
ttggtggac gaaggcgag tgtcccgag ttgggtcta 
gcgatacaa aaccatttc caagcgatt aatatgcgc 
ttgggcaaa actaaaaaa cactccaag catcgacaa 
tcattagtc accctttca cgaaggcat agtttggtg 
aaaatgctt gatgggagt gaattagac ggccctcct 
cccctacgg cgggccagc actgtggag gtgaccacc 
agccccccg tcccaccac accgccaga ctgacccga 
agggcgtct ttggatgac cttgagcag gaatcgtgt 
caattaact ccgaaaata gaccatcga attgtcgtc 
ataacaaac gaggagaaa cgccaggaa ttgattctt 
cagagacag agaatcgag caggactcg atcattgaa 
gaagtcgac gaatattca ccgcagaat taa
    mRNA sequence
    • >TC009234 RA
      tggcacagg cgcactcct cttgacatt tccccaaat 
gtcaacaga tgtcacaca catcctgcg gacaaattt 
aaccgttcg tccttcact ccggccttt tgcaaggat 
gttaaatat ttaatcagt gccgagttt cctcactat 
tccatgact taaccacct ctaaactcg caaaatgga 
atcaacgac caaaaccct aaaccaatc gatgcctcg 
cttataata accctcgtg agtacctcc acctcggag 
cccccacta atccggtgt gtggtatgt tctaacaag 
ggggctatc atggccagt ttcgcggcg caggccgcc 
ctccgtgcg aaatggagc tcgctggtg gaggagcac 
agcatcgag gtgcccccc gaagtcacc agcaaagtg 
acaagtgtg gtgggctgg ctcaaacag gcttcgctg 
gaggttcga gcggccggt cgaagggct gtcaatacg 
ttggccggg ggcatggcg caccatttg gtcattctg 
cacttcaac gatgtgtac aatgttgag ccgagacag 
gggccggag cccgtgggg ggcgcagcc cggttttgc 
acggcgatt aagagtttc cagcacttg catcctctg 
gtgctgttc agcggggac gctttctcg cccagcatg 
ttgagcacc tacacgaaa ggtgagcaa atggtcccc 
gtcctcaac gacctcggc acccactgt gccgtcctc 
ggcaaccac gatttcgat cacggcctt gaagtgctc 
tcccaatgg gtggcccaa accgaattc ccttggctc 
atgtccaac gtactcgat aacgaaacc ggccgtcca 
ttgggcgaa gggcggatc acccatgtg gtacattgg 
gcaggccac agaatcggt cttttgggc cttgttgaa 
aaagaatgg ctggacaca cttgcaacc atcaaccca 
gaaatggtc acttttctc gatttcgtc gaagcggga 
caaaaactc gccgcgcaa ttgaaacaa gaggggtgc 
gattatgtg atcgctctg actcatatg cgaacgcct 
aacgatatc aagctggcg gaaaattgc gataacatc 
gatttgata cttgggggg catgaccac gtgtacgaa 
atcaaggaa atcaacggg aaatttgtg gtgaaaagt 
ggcaccgac tttcggcag tttagtaaa attacggtg 
aattttgat aaaccgaat ggtactcct gaagtaaca 
attgaggaa attaacgtt acttctgcc tttcctgag 
gatccaaaa ttgaaggaa aaactggat aaatacacg 
agtattgtg gaggggaaa atgcacgaa gttttgggt 
tgtttttcc gtgccactt gatggacgt ttcacctcg 
attcgcact tcggaatcg aacttagga aattgggtc 
tgcgatgtg gttttggca gcaacaggt gcggattta 
gtaatttta aattcgggc acttttcgc tcggatcag 
gtgcaccca gctggggac ttcacagtt cgcgatttg 
accaacatt gtgcccatg agagacccc ttggtcatc 
ctcaaatta acaggcaaa caaattttg gaagcgctg 
gaaaatggg gtgtgtatg tacccgaaa ttggagggg 
aggttcccg caagtggcg ggggtgagt tttgccttc 
gacccctcc aaggcacca ggacaacga gtcgaccct 
gcctttgtc cgaatcgga gacgagtat ttgaatttg 
gagcagaat tatcggctt gcgaccaag agttacatg 
cattcaggg tgtgatggt tacgttatg cttaaagat 
gccgaagtt ttggtggac gaaggcgag tgtcccgag 
ttgggtcta gcgatacaa aaccatttc caagcgatt 
aatatgcgc ttgggcaaa actaaaaaa cactccaag 
catcgacaa tcattagtc accctttca cgaaggcat 
agtttggtg aaaatgctt gatgggagt gaattagac 
ggccctcct cccctacgg cgggccagc actgtggag 
gtgaccacc agccccccg tcccaccac accgccaga 
ctgacccga agggcgtct ttggatgac cttgagcag 
gaatcgtgt caattaact ccgaaaata gaccatcga 
attgtcgtc ataacaaac gaggagaaa cgccaggaa 
ttgattctt cagagacag agaatcgag caggactcg 
atcattgaa gaagtcgac gaatattca ccgcagaat 
taattgttt tttttagta gaatttatt tagagtaga 
actcgggta atgccttca aagaacacc acttcaaag 
cattccaca acgagttgg ttgtgaata gaatttttt 
tgagttacc gtttaatca aaatttgta tcaaaagca 
gcaataact aaattctgt gattggcaa gcgtttcat 
ttcgtgttt acttatttg tgcctaaaa cggtcgcaa 
ctttccact aaaactagt acaaatttt attagatgt 
gctgttgtg gtgggcaac ttaatctcg atgcctttg 
cgcacggcc tccttgatg cacagcaaa ctgtcagtt 
tggcagttc aatttgtcg tcaaggggg ctgcgcctg 
tctgtatat ttcttccga tattaatgt gagcaccgt 
cagtgttaa gcttgtcac tgctagtgt atataatgc 
actttttcg aatatttag ccgtcattc aatccgtca 
cttggtatc agacagcgc catctagcg actcactta 
gaccaaacc ccaccttaa gcgtctgat accgattct 
cgtcaattg tgctaatta gtgtgaaag atttcactg 
tgcttcggt ggtaaatgt attgcgaaa cttcagcta 
gtcacaaaa acacctgtt ataaaacac ttagattgt 
tacgaaatt tatttgtgt tgattttta cagttatag 
gtggctgaa tgtagatct gaataaata attttttta 
aac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY