Status
Please wait ...
Gene information
General information
TC009562 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2579414..2583167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009562
    iBeetle screen iB_08640

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009562 RA
      MDHRSPLES IFGIIGGLP QGHDKPVKH ALYNAVALL 
      LLFLCCAAS WALFMILEP FVKPLIWAL LVGSVLHPL 
      KRSLRDKFQ AWFKSLEVS HTPIVLGVM LIPINIVND 
      LSDLIGDQI LKRIKFIIA VCVAVPLVL LVYNYTPKL 
      VVCIFYKAY TYSYFVLGF CLDNASLPL FGLILIAYM 
      SLTFLLWKP ENNTRFHYA SIGIWLLLS CCLAHLFGS 
      FQMAAFIFL QVVFFGGFV SEVYDIHNE LNAEGHSIT 
      FFESLSLAF HDKSLDIDE EHKEPANEA SEQKSEPLE 
      DQDAKPKTS VKFNDGVGD GAPVKSPRR DPYSLDLFE 
      ENKSNLLER TDSELSNAS VLITNQGIH LISKTPKKE 
      LKRSLSQPQ FSLKNRSSF TSLNRRLSS VKSSTQSLI 
      DTEKYESTF YLYSVLWAC IVMLFWKNI VLLPLLPLP 
      ILLYCLKHA GKYFGVWNW LYDKLKIAI LAVSNWCCE 
      RHDALVPVP IRGLYRVVH KINIFLKTS IRESIDTVA 
      SCVVIFGLI IFVTCASVF IAIQIYAEA IMMVQMTGN 
      VINQTVVHN PELRQLLPP AWDDTVDSI LDNAYQYGR 
      EGISKAVKG MMTDVDSAK SEKLEKQVL ELWDRVYQS 
      WMSTTDANG PKVTEDAVR VSWENFISD IQRSPEMFN 
      LNGIVDFAK QNVGTLLSL LESVWSIVK GNIGLVLGS 
      FSAFLSVIL GGGTAVLNF ILNGIVFLT TLFYLLSSS 
      GDLYKPVEL MTKFSTSGR RFGHALEGA INGVFTASF 
      KMAAFYGMW TWFIHNLFG VKIVYLPSA FATILGAVP 
      FLGTYWACF PAVLDLWLA QDRGIEAIV FAIFQFLPT 
      SIVDTTIYK EIKGGGHPY LTGLAIAGG IFCLGVEGA 
      IVGPLLLCG LYVAIDLSS SLFKESPSE EPINLRLQL 
      QDR
    CDS sequence
    • >TC009562 RA
      atggaccac cgttcgccc ttggagagc atcttcggc 
      ataatcggg gggctgccg caggggcat gacaagccc 
      gtcaagcat gccctttac aacgccgtg gcccttctg 
      ctgctgttc ctgtgttgt gcggccagt tgggccctg 
      ttcatgatc ctggagccg ttcgtgaag cccctgatc 
      tgggccttg ctggtgggg tcggtgttg caccccctt 
      aagcggtcg ctcagggac aagttccag gcctggttc 
      aagagtctg gaggtgtct cacaccccc attgtgctg 
      ggggtgatg ctcatccct atcaacata gtcaacgat 
      ttgtcggat ttgatcggt gaccaaatt ttgaaacgg 
      atcaagttc attatcgct gtttgtgtg gcggtgcct 
      ttggtgctc ctggtctat aattacacg ccaaagttg 
      gtggtttgc attttttat aaagcttac acttatagc 
      tacttcgta ctgggtttt tgtctggat aatgcctct 
      ctacctctg tttggccta attttaatc gcctacatg 
      tctctgaca ttcctgctg tggaaaccc gaaaataat 
      acgcgattt cattacgca tcaattgga atttggttg 
      ttgttgtct tgttgtttg gctcattta ttcgggtcg 
      ttccaaatg gctgcgttt attttttta caagttgtg 
      tttttcggc ggatttgtg agcgaagtc tacgacatt 
      cataacgaa ctaaacgct gaaggccac tcaattaca 
      ttctttgag tcgctatct cttgcattc catgacaag 
      tccttagat attgacgag gaacataaa gaaccagcg 
      aacgaagcg agtgaacaa aagtcggaa cctctagag 
      gatcaggat gccaagccg aagacttcg gtcaagttc 
      aacgatggt gtgggcgac ggcgccccc gtaaaatcc 
      ccccgaaga gacccctac tctctcgat ttattcgag 
      gagaacaaa tcgaatctc ctcgagcga actgactcc 
      gagttgagc aatgcgagt gtcctaata accaatcag 
      gggattcac ctgatttca aaaaccccg aaaaaagaa 
      ctgaaacgt tcgttgtcc caaccccag ttctcgctg 
      aaaaatcgc tcgagtttc acgagtttg aatcggcga 
      ttgagtagt gtgaaaagt tcaacgcaa agtctgata 
      gataccgaa aagtacgag agtactttt tacctgtat 
      tcggtactt tgggcttgc attgtgatg ttgttttgg 
      aaaaatatc gtcttgttg ccgttgcta ccgcttccg 
      attttgttg tactgtctc aagcatgcc gggaaatat 
      ttcggtgtg tggaattgg ctttatgat aagttgaaa 
      attgcgatc ttggctgtt agtaactgg tgttgtgag 
      agacacgac gcccttgtt cctgtgcca attaggggc 
      ctctatcga gtggtccac aaaataaat atttttctc 
      aaaactagt attagagag agtattgat acggtggcg 
      agttgtgtc gttattttt ggtcttatt attttcgtg 
      acttgtgcg tcagttttt atagcaatt cagatctat 
      gccgaggct attatgatg gttcaaatg accggaaat 
      gtgatcaat cagactgtt gttcacaac ccagagttg 
      agacagctt ttgcccccc gcttgggac gacactgtt 
      gactccatt ttggacaat gcgtaccaa tacggccga 
      gaaggaatc tccaaagcg gtcaaagga atgatgaca 
      gacgtggac tcggcaaag tcggaaaaa ctggaaaag 
      caagtgttg gaattatgg gatcgtgtc tatcagtcc 
      tggatgtcc acaaccgac gcaaacggc cctaaagtg 
      accgaagac gcggtccga gtgtcttgg gaaaatttc 
      atcagtgac atacagaga tcaccagaa atgttcaac 
      ttgaacgga atcgtggat tttgcgaaa caaaacgtg 
      gggacactt ctgtccctt ctggaatca gtctggtcc 
      atagttaag ggaaacatc gggctagtg cttggctca 
      tttagcgcc tttttatcg gtgattttg ggcggcggc 
      accgcggtg ctaaacttc atcttgaat gggattgtg 
      ttcctcaca actttgttt tacctcctg agttcaagt 
      ggcgacctt tacaaacca gtcgaactc atgacgaag 
      ttttcgacc agtgggcgc cgctttggc cacgctttg 
      gagggggct atcaacggg gtctttacc gcctcgttc 
      aaaatggca gcgttttac ggcatgtgg acgtggttc 
      atccacaat ttattcggc gtgaaaatc gtctatctt 
      ccgtcagct tttgcgaca attctgggg gctgtccct 
      tttttgggg acctactgg gcgtgtttt cccgccgtt 
      ttggacttg tggttggcc caagaccgc gggattgag 
      gcaattgtg tttgccatt ttccagttt ttgccaact 
      tcgatcgtt gatactacc atttataag gaaatcaaa 
      gggggcggt cacccgtat ttgaccggt ttggccatc 
      gctgggggg attttctgt ctcggggtc gagggggct 
      atcgtgggg ccccttcta ctgtgcggg ctgtacgtc 
      gcgattgac ttgtcttct agtcttttt aaggagtct 
      ccctcagag gagccgatc aatctcagg ttgcagctg 
      caagaccga taa
    mRNA sequence
    • >TC009562 RA
      gtgaaattt tcaaattta ttaattttt atgaataac 
      aagatctta tgaaactat ttagacaag tgctctgga 
      agcattaca tcgtcttac actaattgt ggttgactt 
      tctttaaaa atcgggcat agtttgacc tggtttgcc 
      actgacagt tgacaaata caaacagcg tgaccttag 
      ccactcacg tgtcccttc tgtcagcga tgtcaacat 
      tgctctaaa attaaaaat gattcgata aatttaatc 
      agttgaatt aatatcgcg atggaccac cgttcgccc 
      ttggagagc atcttcggc ataatcggg gggctgccg 
      caggggcat gacaagccc gtcaagcat gccctttac 
      aacgccgtg gcccttctg ctgctgttc ctgtgttgt 
      gcggccagt tgggccctg ttcatgatc ctggagccg 
      ttcgtgaag cccctgatc tgggccttg ctggtgggg 
      tcggtgttg caccccctt aagcggtcg ctcagggac 
      aagttccag gcctggttc aagagtctg gaggtgtct 
      cacaccccc attgtgctg ggggtgatg ctcatccct 
      atcaacata gtcaacgat ttgtcggat ttgatcggt 
      gaccaaatt ttgaaacgg atcaagttc attatcgct 
      gtttgtgtg gcggtgcct ttggtgctc ctggtctat 
      aattacacg ccaaagttg gtggtttgc attttttat 
      aaagcttac acttatagc tacttcgta ctgggtttt 
      tgtctggat aatgcctct ctacctctg tttggccta 
      attttaatc gcctacatg tctctgaca ttcctgctg 
      tggaaaccc gaaaataat acgcgattt cattacgca 
      tcaattgga atttggttg ttgttgtct tgttgtttg 
      gctcattta ttcgggtcg ttccaaatg gctgcgttt 
      attttttta caagttgtg tttttcggc ggatttgtg 
      agcgaagtc tacgacatt cataacgaa ctaaacgct 
      gaaggccac tcaattaca ttctttgag tcgctatct 
      cttgcattc catgacaag tccttagat attgacgag 
      gaacataaa gaaccagcg aacgaagcg agtgaacaa 
      aagtcggaa cctctagag gatcaggat gccaagccg 
      aagacttcg gtcaagttc aacgatggt gtgggcgac 
      ggcgccccc gtaaaatcc ccccgaaga gacccctac 
      tctctcgat ttattcgag gagaacaaa tcgaatctc 
      ctcgagcga actgactcc gagttgagc aatgcgagt 
      gtcctaata accaatcag gggattcac ctgatttca 
      aaaaccccg aaaaaagaa ctgaaacgt tcgttgtcc 
      caaccccag ttctcgctg aaaaatcgc tcgagtttc 
      acgagtttg aatcggcga ttgagtagt gtgaaaagt 
      tcaacgcaa agtctgata gataccgaa aagtacgag 
      agtactttt tacctgtat tcggtactt tgggcttgc 
      attgtgatg ttgttttgg aaaaatatc gtcttgttg 
      ccgttgcta ccgcttccg attttgttg tactgtctc 
      aagcatgcc gggaaatat ttcggtgtg tggaattgg 
      ctttatgat aagttgaaa attgcgatc ttggctgtt 
      agtaactgg tgttgtgag agacacgac gcccttgtt 
      cctgtgcca attaggggc ctctatcga gtggtccac 
      aaaataaat atttttctc aaaactagt attagagag 
      agtattgat acggtggcg agttgtgtc gttattttt 
      ggtcttatt attttcgtg acttgtgcg tcagttttt 
      atagcaatt cagatctat gccgaggct attatgatg 
      gttcaaatg accggaaat gtgatcaat cagactgtt 
      gttcacaac ccagagttg agacagctt ttgcccccc 
      gcttgggac gacactgtt gactccatt ttggacaat 
      gcgtaccaa tacggccga gaaggaatc tccaaagcg 
      gtcaaagga atgatgaca gacgtggac tcggcaaag 
      tcggaaaaa ctggaaaag caagtgttg gaattatgg 
      gatcgtgtc tatcagtcc tggatgtcc acaaccgac 
      gcaaacggc cctaaagtg accgaagac gcggtccga 
      gtgtcttgg gaaaatttc atcagtgac atacagaga 
      tcaccagaa atgttcaac ttgaacgga atcgtggat 
      tttgcgaaa caaaacgtg gggacactt ctgtccctt 
      ctggaatca gtctggtcc atagttaag ggaaacatc 
      gggctagtg cttggctca tttagcgcc tttttatcg 
      gtgattttg ggcggcggc accgcggtg ctaaacttc 
      atcttgaat gggattgtg ttcctcaca actttgttt 
      tacctcctg agttcaagt ggcgacctt tacaaacca 
      gtcgaactc atgacgaag ttttcgacc agtgggcgc 
      cgctttggc cacgctttg gagggggct atcaacggg 
      gtctttacc gcctcgttc aaaatggca gcgttttac 
      ggcatgtgg acgtggttc atccacaat ttattcggc 
      gtgaaaatc gtctatctt ccgtcagct tttgcgaca 
      attctgggg gctgtccct tttttgggg acctactgg 
      gcgtgtttt cccgccgtt ttggacttg tggttggcc 
      caagaccgc gggattgag gcaattgtg tttgccatt 
      ttccagttt ttgccaact tcgatcgtt gatactacc 
      atttataag gaaatcaaa gggggcggt cacccgtat 
      ttgaccggt ttggccatc gctgggggg attttctgt 
      ctcggggtc gagggggct atcgtgggg ccccttcta 
      ctgtgcggg ctgtacgtc gcgattgac ttgtcttct 
      agtcttttt aaggagtct ccctcagag gagccgatc 
      aatctcagg ttgcagctg caagaccga taattgttc 
      cagaagttt ataatgctt aatgttatt gtgcaccga 
      gggctttaa aacacgtta cctaaccaa gatttttac 
      agatattta ttgatgttt aagattgtt agatgtttt 
      taagaaata aacgaaaat caaaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY