General information | |||
TC009562 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2579414..2583167 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009562 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08640 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009562 RA
MDHRSPLES IFGIIGGLP QGHDKPVKH ALYNAVALL LLFLCCAAS WALFMILEP FVKPLIWAL LVGSVLHPL KRSLRDKFQ AWFKSLEVS HTPIVLGVM LIPINIVND LSDLIGDQI LKRIKFIIA VCVAVPLVL LVYNYTPKL VVCIFYKAY TYSYFVLGF CLDNASLPL FGLILIAYM SLTFLLWKP ENNTRFHYA SIGIWLLLS CCLAHLFGS FQMAAFIFL QVVFFGGFV SEVYDIHNE LNAEGHSIT FFESLSLAF HDKSLDIDE EHKEPANEA SEQKSEPLE DQDAKPKTS VKFNDGVGD GAPVKSPRR DPYSLDLFE ENKSNLLER TDSELSNAS VLITNQGIH LISKTPKKE LKRSLSQPQ FSLKNRSSF TSLNRRLSS VKSSTQSLI DTEKYESTF YLYSVLWAC IVMLFWKNI VLLPLLPLP ILLYCLKHA GKYFGVWNW LYDKLKIAI LAVSNWCCE RHDALVPVP IRGLYRVVH KINIFLKTS IRESIDTVA SCVVIFGLI IFVTCASVF IAIQIYAEA IMMVQMTGN VINQTVVHN PELRQLLPP AWDDTVDSI LDNAYQYGR EGISKAVKG MMTDVDSAK SEKLEKQVL ELWDRVYQS WMSTTDANG PKVTEDAVR VSWENFISD IQRSPEMFN LNGIVDFAK QNVGTLLSL LESVWSIVK GNIGLVLGS FSAFLSVIL GGGTAVLNF ILNGIVFLT TLFYLLSSS GDLYKPVEL MTKFSTSGR RFGHALEGA INGVFTASF KMAAFYGMW TWFIHNLFG VKIVYLPSA FATILGAVP FLGTYWACF PAVLDLWLA QDRGIEAIV FAIFQFLPT SIVDTTIYK EIKGGGHPY LTGLAIAGG IFCLGVEGA IVGPLLLCG LYVAIDLSS SLFKESPSE EPINLRLQL QDR
- >TC009562 RA
atggaccac cgttcgccc ttggagagc atcttcggc ataatcggg gggctgccg caggggcat gacaagccc gtcaagcat gccctttac aacgccgtg gcccttctg ctgctgttc ctgtgttgt gcggccagt tgggccctg ttcatgatc ctggagccg ttcgtgaag cccctgatc tgggccttg ctggtgggg tcggtgttg caccccctt aagcggtcg ctcagggac aagttccag gcctggttc aagagtctg gaggtgtct cacaccccc attgtgctg ggggtgatg ctcatccct atcaacata gtcaacgat ttgtcggat ttgatcggt gaccaaatt ttgaaacgg atcaagttc attatcgct gtttgtgtg gcggtgcct ttggtgctc ctggtctat aattacacg ccaaagttg gtggtttgc attttttat aaagcttac acttatagc tacttcgta ctgggtttt tgtctggat aatgcctct ctacctctg tttggccta attttaatc gcctacatg tctctgaca ttcctgctg tggaaaccc gaaaataat acgcgattt cattacgca tcaattgga atttggttg ttgttgtct tgttgtttg gctcattta ttcgggtcg ttccaaatg gctgcgttt attttttta caagttgtg tttttcggc ggatttgtg agcgaagtc tacgacatt cataacgaa ctaaacgct gaaggccac tcaattaca ttctttgag tcgctatct cttgcattc catgacaag tccttagat attgacgag gaacataaa gaaccagcg aacgaagcg agtgaacaa aagtcggaa cctctagag gatcaggat gccaagccg aagacttcg gtcaagttc aacgatggt gtgggcgac ggcgccccc gtaaaatcc ccccgaaga gacccctac tctctcgat ttattcgag gagaacaaa tcgaatctc ctcgagcga actgactcc gagttgagc aatgcgagt gtcctaata accaatcag gggattcac ctgatttca aaaaccccg aaaaaagaa ctgaaacgt tcgttgtcc caaccccag ttctcgctg aaaaatcgc tcgagtttc acgagtttg aatcggcga ttgagtagt gtgaaaagt tcaacgcaa agtctgata gataccgaa aagtacgag agtactttt tacctgtat tcggtactt tgggcttgc attgtgatg ttgttttgg aaaaatatc gtcttgttg ccgttgcta ccgcttccg attttgttg tactgtctc aagcatgcc gggaaatat ttcggtgtg tggaattgg ctttatgat aagttgaaa attgcgatc ttggctgtt agtaactgg tgttgtgag agacacgac gcccttgtt cctgtgcca attaggggc ctctatcga gtggtccac aaaataaat atttttctc aaaactagt attagagag agtattgat acggtggcg agttgtgtc gttattttt ggtcttatt attttcgtg acttgtgcg tcagttttt atagcaatt cagatctat gccgaggct attatgatg gttcaaatg accggaaat gtgatcaat cagactgtt gttcacaac ccagagttg agacagctt ttgcccccc gcttgggac gacactgtt gactccatt ttggacaat gcgtaccaa tacggccga gaaggaatc tccaaagcg gtcaaagga atgatgaca gacgtggac tcggcaaag tcggaaaaa ctggaaaag caagtgttg gaattatgg gatcgtgtc tatcagtcc tggatgtcc acaaccgac gcaaacggc cctaaagtg accgaagac gcggtccga gtgtcttgg gaaaatttc atcagtgac atacagaga tcaccagaa atgttcaac ttgaacgga atcgtggat tttgcgaaa caaaacgtg gggacactt ctgtccctt ctggaatca gtctggtcc atagttaag ggaaacatc gggctagtg cttggctca tttagcgcc tttttatcg gtgattttg ggcggcggc accgcggtg ctaaacttc atcttgaat gggattgtg ttcctcaca actttgttt tacctcctg agttcaagt ggcgacctt tacaaacca gtcgaactc atgacgaag ttttcgacc agtgggcgc cgctttggc cacgctttg gagggggct atcaacggg gtctttacc gcctcgttc aaaatggca gcgttttac ggcatgtgg acgtggttc atccacaat ttattcggc gtgaaaatc gtctatctt ccgtcagct tttgcgaca attctgggg gctgtccct tttttgggg acctactgg gcgtgtttt cccgccgtt ttggacttg tggttggcc caagaccgc gggattgag gcaattgtg tttgccatt ttccagttt ttgccaact tcgatcgtt gatactacc atttataag gaaatcaaa gggggcggt cacccgtat ttgaccggt ttggccatc gctgggggg attttctgt ctcggggtc gagggggct atcgtgggg ccccttcta ctgtgcggg ctgtacgtc gcgattgac ttgtcttct agtcttttt aaggagtct ccctcagag gagccgatc aatctcagg ttgcagctg caagaccga taa
- >TC009562 RA
gtgaaattt tcaaattta ttaattttt atgaataac aagatctta tgaaactat ttagacaag tgctctgga agcattaca tcgtcttac actaattgt ggttgactt tctttaaaa atcgggcat agtttgacc tggtttgcc actgacagt tgacaaata caaacagcg tgaccttag ccactcacg tgtcccttc tgtcagcga tgtcaacat tgctctaaa attaaaaat gattcgata aatttaatc agttgaatt aatatcgcg atggaccac cgttcgccc ttggagagc atcttcggc ataatcggg gggctgccg caggggcat gacaagccc gtcaagcat gccctttac aacgccgtg gcccttctg ctgctgttc ctgtgttgt gcggccagt tgggccctg ttcatgatc ctggagccg ttcgtgaag cccctgatc tgggccttg ctggtgggg tcggtgttg caccccctt aagcggtcg ctcagggac aagttccag gcctggttc aagagtctg gaggtgtct cacaccccc attgtgctg ggggtgatg ctcatccct atcaacata gtcaacgat ttgtcggat ttgatcggt gaccaaatt ttgaaacgg atcaagttc attatcgct gtttgtgtg gcggtgcct ttggtgctc ctggtctat aattacacg ccaaagttg gtggtttgc attttttat aaagcttac acttatagc tacttcgta ctgggtttt tgtctggat aatgcctct ctacctctg tttggccta attttaatc gcctacatg tctctgaca ttcctgctg tggaaaccc gaaaataat acgcgattt cattacgca tcaattgga atttggttg ttgttgtct tgttgtttg gctcattta ttcgggtcg ttccaaatg gctgcgttt attttttta caagttgtg tttttcggc ggatttgtg agcgaagtc tacgacatt cataacgaa ctaaacgct gaaggccac tcaattaca ttctttgag tcgctatct cttgcattc catgacaag tccttagat attgacgag gaacataaa gaaccagcg aacgaagcg agtgaacaa aagtcggaa cctctagag gatcaggat gccaagccg aagacttcg gtcaagttc aacgatggt gtgggcgac ggcgccccc gtaaaatcc ccccgaaga gacccctac tctctcgat ttattcgag gagaacaaa tcgaatctc ctcgagcga actgactcc gagttgagc aatgcgagt gtcctaata accaatcag gggattcac ctgatttca aaaaccccg aaaaaagaa ctgaaacgt tcgttgtcc caaccccag ttctcgctg aaaaatcgc tcgagtttc acgagtttg aatcggcga ttgagtagt gtgaaaagt tcaacgcaa agtctgata gataccgaa aagtacgag agtactttt tacctgtat tcggtactt tgggcttgc attgtgatg ttgttttgg aaaaatatc gtcttgttg ccgttgcta ccgcttccg attttgttg tactgtctc aagcatgcc gggaaatat ttcggtgtg tggaattgg ctttatgat aagttgaaa attgcgatc ttggctgtt agtaactgg tgttgtgag agacacgac gcccttgtt cctgtgcca attaggggc ctctatcga gtggtccac aaaataaat atttttctc aaaactagt attagagag agtattgat acggtggcg agttgtgtc gttattttt ggtcttatt attttcgtg acttgtgcg tcagttttt atagcaatt cagatctat gccgaggct attatgatg gttcaaatg accggaaat gtgatcaat cagactgtt gttcacaac ccagagttg agacagctt ttgcccccc gcttgggac gacactgtt gactccatt ttggacaat gcgtaccaa tacggccga gaaggaatc tccaaagcg gtcaaagga atgatgaca gacgtggac tcggcaaag tcggaaaaa ctggaaaag caagtgttg gaattatgg gatcgtgtc tatcagtcc tggatgtcc acaaccgac gcaaacggc cctaaagtg accgaagac gcggtccga gtgtcttgg gaaaatttc atcagtgac atacagaga tcaccagaa atgttcaac ttgaacgga atcgtggat tttgcgaaa caaaacgtg gggacactt ctgtccctt ctggaatca gtctggtcc atagttaag ggaaacatc gggctagtg cttggctca tttagcgcc tttttatcg gtgattttg ggcggcggc accgcggtg ctaaacttc atcttgaat gggattgtg ttcctcaca actttgttt tacctcctg agttcaagt ggcgacctt tacaaacca gtcgaactc atgacgaag ttttcgacc agtgggcgc cgctttggc cacgctttg gagggggct atcaacggg gtctttacc gcctcgttc aaaatggca gcgttttac ggcatgtgg acgtggttc atccacaat ttattcggc gtgaaaatc gtctatctt ccgtcagct tttgcgaca attctgggg gctgtccct tttttgggg acctactgg gcgtgtttt cccgccgtt ttggacttg tggttggcc caagaccgc gggattgag gcaattgtg tttgccatt ttccagttt ttgccaact tcgatcgtt gatactacc atttataag gaaatcaaa gggggcggt cacccgtat ttgaccggt ttggccatc gctgggggg attttctgt ctcggggtc gagggggct atcgtgggg ccccttcta ctgtgcggg ctgtacgtc gcgattgac ttgtcttct agtcttttt aaggagtct ccctcagag gagccgatc aatctcagg ttgcagctg caagaccga taattgttc cagaagttt ataatgctt aatgttatt gtgcaccga gggctttaa aacacgtta cctaaccaa gatttttac agatattta ttgatgttt aagattgtt agatgtttt taagaaata aacgaaaat caaaaga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
