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Gene information
General information
TC009560 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2544508..2562187
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009560
    iBeetle screen iB_08638

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    maxilla bristle position ventralized - Penetrance (percentage of animals/eggs on slide): more than 80%
    head cuticle lateral ventralconstricted - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen tracheal opening present - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Tracheal system disrupted, but tracheal openings not affected
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009560 RA
      MQGVLFMPQ NPQQGSPPP NNALVTDIT MEVEPESTK 
      QSPTNQSTQ SDSGIFSAT NTTATMDPC DTDNRSDCS 
      SPERKYVCP ICDTMLTSQ HEFTLHIRS HNNETEAQD 
      DKGFTCRIC FKVLSSSSS LDRHVLVHS GERPFHCRY 
      CGDTFTTNG NMHRHMRTH SHKADNYES DASTDSGSS 
      KSTEFNNNK VESKKRKLE ASDHANKRT ENASDCESQ 
      FYKCPVCER TDFDSIVAL EAHLEDNHP DYPAKCGQC 
      NQLFQNNKL LTIHRNTVH SSGNKRHTV AGFKDLTFV 
      DFSSEKFPY IARYECEKN LHTASDGLT FQCKTCTRA 
      FPCSSSLEI HQKDCQNQY NGLDLTKTQ VSDKEIRRN 
      EFFARLNLQ DNSPEKFVP PPQISETST KLRQQLLAK 
      VMTNDSKDL ADIQSIISM TTNGSLLQQ LQTKSDLAQ 
      QETQRSEQE EESQDLFAV EFRKMKLRG EFPCRLCTA 
      VFPNLRALK GHNRAHLNG NNNGMYRCN MCPHSSIDK 
      AALIRHMRT HNGDRPYEC SLCNYAFTT KANCERHLR 
      NRHAKTTRE EVKKAIIYH PSEDPTNED LNKLAEPRK 
      SDLVPTPEK HKPEIAPLI PTLLKETLN VPLPKEPPL 
      FSNIIRSEN LKSLKEHRT NGQFSPYLP PYNFTPKPT 
      NVSTPHSKI QVKNLEELK EPSDLEEEQ EEEPMNLVL 
      DLSKKKEVK PEVPQDLSK KTPPLQMRD LFAQQLLKT 
      PPKIDPATL YASIYRNTF PGFPGWPLN PLLFSSLQT 
      PVLPPESDM KERLQRFQF CGGSMISDN FRMQTPIEL 
      KKTEPLSLN VEANFGDNY KLQSPVAKP DLTQSPNSV 
      KMVIKNGVL MPKQKQRRY RTERPFTCE HCSARFTLR 
      SNMERHIKQ QHPQFWSQR QRGVSNPGR KSQMKSYCD 
      LSIPNYEVP KSQDYDDTK EHMEEKLKF AILAQHLRV 
      GQEVKKDDD EDGALVIDE NAEKAEKTE DSRCYETGF 
      ERSKILEEK LLELRAKQI KSEKVKNEE DLVPVSRLL 
      DNASQQQFK EYFKREGEE PEVGGASEE DEEGLVASG 
      STSEGNISG TDENKSESE TAAPAKKKS AYSMAPNRV 
      SCPFCSRKF PWSSSLRRH ILTHTGQKP FKCSHCPLL 
      FTTKSNCDR HLLRKHGNN ATTITNDAV NNNTNYLMR 
      NVPERPFKC SNCPSSTFS TYSNLKKHI SCKHSGDDI 
      KAQGYEAGS SEDEKLPTN ESKTDWDEG KKSEVQNNV 
      VQNSDLPFK CHLCDGSFG ERQDALDHI KDKHYAEYD 
      LLMSKNALD NNATTPEEG GHHEDEEND MRGKFPDYS 
      NRKVICAFC MRRFWSAED LRRHMRTHT GERPFSCDI 
      CRRRFTLKH SMLRHRKKH NVNFDSEIV GSDDDNNAP 
      NFAKTENNN TQKEESDGN EGSDLISKL LGIRDRSII 
      DKVLTASAD DAAKLLGVK NGEKE
    CDS sequence
    • >TC009560 RA
      atgcaaggg gtgctcttc atgccgcag aatccacag 
      cagggctcg ccgccgcca aacaacgct ctagtaacc 
      gacattacc atggaggta gaacccgaa tcaacgaaa 
      caatcccca acgaaccaa agcacccaa agcgactct 
      ggcatcttc tccgccacc aacaccacc gccaccatg 
      gacccctgc gacaccgac aaccgcagc gactgcagc 
      tcccccgag cgcaaatac gtctgcccg atctgcgac 
      accatgctg acctcccag cacgagttc accctccat 
      atccgatct cacaacaac gaaacggag gcccaggac 
      gacaagggc ttcacctgt cgcatttgc ttcaaagtg 
      ctaagttcc agctcgagc ctggaccgc cacgtgctg 
      gtccacagc ggcgaacgc cccttccat tgccgctac 
      tgcggcgac accttcacc accaacgga aacatgcac 
      cgacacatg cggacacat tcccacaaa gcggacaac 
      tacgaatcc gacgccagc accgacagc ggaagctcc 
      aaaagcacc gaattcaac aacaacaaa gtcgaatcg 
      aaaaaacgc aagctggag gcgagcgac cacgcgaat 
      aagcgaacg gagaacgcg agcgattgc gaaagccag 
      ttctacaag tgcccggtt tgcgaacgc accgacttt 
      gactcgatt gtggcgctg gaagcgcac ctggaggac 
      aatcacccg gattatccc gccaaatgt gggcaatgc 
      aaccaactc ttccaaaac aacaaactc ctaactatc 
      catcggaac actgtacat tccagcggg aataaaaga 
      cacacagtt gccgggttc aaagacttg acttttgtc 
      gacttttcc agcgagaag tttccatat atcgcgcgg 
      tacgaatgc gagaaaaac ctacacacg gcttccgac 
      ggactcact ttccagtgc aagacctgc acacgggcg 
      tttccctgc tccagctcg ctggaaatc caccagaaa 
      gactgccag aaccaatac aatgggctt gacttgacc 
      aagacgcaa gtctccgac aaggaaatc cggcggaac 
      gagtttttc gccagattg aaccttcag gacaattcg 
      ccggaaaag ttcgtccct ccgccacag atcagcgaa 
      acttcgacc aaactccga cagcaactt ttggcgaaa 
      gttatgacc aatgactcg aaggatctg gcggacatc 
      cagtccatt atttcgatg actacgaac ggtagtctc 
      ttgcaacaa ctccagacc aagagcgat ttggcgcaa 
      caagagact caaaggtcg gaacaagag gaggaatct 
      caggatttg ttcgccgtg gagtttcgc aaaatgaag 
      ctccggggc gagttcccg tgccgtctc tgcacggcg 
      gttttcccg aatctgagg gccttaaaa gggcacaat 
      agggcccat cttaatggt aataacaat gggatgtat 
      cgctgcaac atgtgccca cactcatcc atcgataaa 
      gcggcgctg atcagacac atgaggacc cataatggg 
      gacagaccg tacgaatgc tctctctgt aactacgct 
      ttcaccacc aaggccaat tgtgagcgg cacttgagg 
      aacagacac gccaagaca acacgtgag gaggtcaag 
      aaagctata atctatcac ccgtcggaa gaccccacc 
      aatgaggat ttaaacaaa ctggcggaa ccacgcaaa 
      agtgacttg gtaccaacc cctgagaaa cacaaacct 
      gagatagcg cccctaatt cccacttta ctcaaggag 
      acactcaat gtccccctc cctaaggaa cctcccctt 
      ttctcaaac atcatccgg agcgagaat ttgaaaagt 
      ttgaaggaa cacaggact aatgggcaa ttttcgccc 
      tatttgccg ccttacaac ttcacacca aaacctaca 
      aacgtctca accccccat tcaaaaatc caggtcaag 
      aacctggag gagctgaag gagccctcc gacttggag 
      gaggagcag gaggaggaa ccaatgaac ttggtcctt 
      gatttgagc aagaagaaa gaggtcaag ccggaagta 
      ccgcaagac ctgtcaaag aaaaccccc ccgttgcaa 
      atgcgcgat ttattcgcc caacagtta ctcaaaact 
      ccgccaaaa atcgaccct gcaactctc tacgcctcg 
      atttaccgc aacaccttc ccgggattc cccggctgg 
      cctttaaac ccgctcctg ttttcctcc ctccagacc 
      ccggtactc cccccggag tcggacatg aaggagcgc 
      ttgcagcgc ttccagttc tgcggcggc tccatgatc 
      agtgacaac ttcaggatg caaaccccc atcgaactg 
      aagaaaacc gagcctttg agtctcaac gtggaggcg 
      aactttggc gacaactac aagttgcaa agtccggtc 
      gcgaagccc gacctcacc cagtcgccc aattcggtc 
      aagatggtg atcaagaac ggggttttg atgccgaag 
      cagaaacag aggcggtat cggaccgaa cgtcccttc 
      acctgcgag cattgctcg gctaggttt acgttaagg 
      tcgaacatg gagagacac atcaagcag cagcatccc 
      cagttttgg tcgcagcgc cagagaggc gtgagcaac 
      ccggggcgc aaaagccag atgaagagt tactgtgat 
      ctcagtata ccaaactac gaagtacca aagagtcag 
      gattatgac gataccaaa gaacacatg gaggagaag 
      ttgaagttt gcgattctg gcgcagcat ttgagggtc 
      gggcaggag gtgaagaag gacgatgat gaggatggg 
      gcgcttgtg atcgacgag aatgccgag aaggccgaa 
      aagactgag gatagtagg tgttacgag acggggttt 
      gaacggagt aagattctc gaggagaag ttgctagag 
      ttgcgtgcg aagcagatc aagtcggag aaggtgaag 
      aatgaggag gatttggtg ccggtgtcc aggcttctg 
      gataatgcg tcacaacaa cagttcaag gagtatttc 
      aagagagag ggcgaggag ccggaggtt ggaggagcg 
      agtgaggag gatgaggaa gggctagtg gccagtggg 
      agcacgagc gaagggaac atctcagga acggacgaa 
      aataaatcc gagtcggag accgccgcc cccgccaag 
      aagaagtcc gcctactcc atggccccc aaccgcgtg 
      agctgcccc ttctgcagc cgcaaattc ccgtggagc 
      tcttcgttg cgccgccac atcctcacc cacaccggc 
      cagaagccg ttcaaatgc agccactgt cctctcctc 
      ttcaccacc aagtccaac tgcgaccgc cacttgctg 
      cgcaaacac ggcaacaac gccaccacc atcaccaac 
      gacgccgtc aacaacaac accaactat ctgatgcgc 
      aacgtgccc gaacgcccc ttcaagtgc tccaactgc 
      cccagcagc accttctcc acctactcc aacctgaag 
      aagcacatc agctgcaag cactccggc gacgacatc 
      aaggcccaa ggctacgaa gccggcagt tccgaggac 
      gagaaactg ccaacaaac gagtccaaa accgactgg 
      gacgagggc aaaaaatcc gaagtccag aataacgtg 
      gtgcagaat tccgatcta ccgttcaag tgccatttg 
      tgcgatggc tcgtttggg gagcgtcaa gatgcattg 
      gaccacatc aaggacaag cattacgca gagtacgac 
      ctgctaatg tccaaaaat gcgctagat aacaacgcc 
      acaaccccc gaagaaggg ggccaccat gaggacgag 
      gagaacgac atgaggggg aaattcccg gattattcc 
      aacaggaag gtgatttgc gccttctgc atgcgtcgc 
      ttctggtcg gcggaggat ctcagaagg cacatgagg 
      acccacacg ggggagcgc cccttcagc tgtgacata 
      tgccggcgc agattcacc ctcaagcat agcatgttg 
      cgccaccga aagaaacac aacgtgaac ttcgacagc 
      gaaatcgtc ggaagcgac gacgataac aacgcccca 
      aatttcgca aaaacggaa aataacaac acccagaaa 
      gaggagtcg gacgggaac gagggtagc gacttgatt 
      agtaagtta ttagggatc agggatagg tctataatt 
      gataaggtt ctgacggcg tctgccgac gacgccgcc 
      aagctccta ggagtcaaa aacggggag aaagaatag
    mRNA sequence
    • >TC009560 RA
      ccgctcacc cacagccag gcgacttga tctttttaa 
      gctcacttc gcgttccat gtcttgctc gcgagcatc 
      acatcgaga gtgctggtt gatgagttc gaatttgat 
      gacttgtga cgatcaatt gggacaaaa tcggtgcgg 
      atcagtggc gtggtgcac ttgtgatgg attaccttt 
      ccccaactc cgagaacgt aatttcgaa atgcaaggg 
      gtgctcttc atgccgcag aatccacag cagggctcg 
      ccgccgcca aacaacgct ctagtaacc gacattacc 
      atggaggta gaacccgaa tcaacgaaa caatcccca 
      acgaaccaa agcacccaa agcgactct ggcatcttc 
      tccgccacc aacaccacc gccaccatg gacccctgc 
      gacaccgac aaccgcagc gactgcagc tcccccgag 
      cgcaaatac gtctgcccg atctgcgac accatgctg 
      acctcccag cacgagttc accctccat atccgatct 
      cacaacaac gaaacggag gcccaggac gacaagggc 
      ttcacctgt cgcatttgc ttcaaagtg ctaagttcc 
      agctcgagc ctggaccgc cacgtgctg gtccacagc 
      ggcgaacgc cccttccat tgccgctac tgcggcgac 
      accttcacc accaacgga aacatgcac cgacacatg 
      cggacacat tcccacaaa gcggacaac tacgaatcc 
      gacgccagc accgacagc ggaagctcc aaaagcacc 
      gaattcaac aacaacaaa gtcgaatcg aaaaaacgc 
      aagctggag gcgagcgac cacgcgaat aagcgaacg 
      gagaacgcg agcgattgc gaaagccag ttctacaag 
      tgcccggtt tgcgaacgc accgacttt gactcgatt 
      gtggcgctg gaagcgcac ctggaggac aatcacccg 
      gattatccc gccaaatgt gggcaatgc aaccaactc 
      ttccaaaac aacaaactc ctaactatc catcggaac 
      actgtacat tccagcggg aataaaaga cacacagtt 
      gccgggttc aaagacttg acttttgtc gacttttcc 
      agcgagaag tttccatat atcgcgcgg tacgaatgc 
      gagaaaaac ctacacacg gcttccgac ggactcact 
      ttccagtgc aagacctgc acacgggcg tttccctgc 
      tccagctcg ctggaaatc caccagaaa gactgccag 
      aaccaatac aatgggctt gacttgacc aagacgcaa 
      gtctccgac aaggaaatc cggcggaac gagtttttc 
      gccagattg aaccttcag gacaattcg ccggaaaag 
      ttcgtccct ccgccacag atcagcgaa acttcgacc 
      aaactccga cagcaactt ttggcgaaa gttatgacc 
      aatgactcg aaggatctg gcggacatc cagtccatt 
      atttcgatg actacgaac ggtagtctc ttgcaacaa 
      ctccagacc aagagcgat ttggcgcaa caagagact 
      caaaggtcg gaacaagag gaggaatct caggatttg 
      ttcgccgtg gagtttcgc aaaatgaag ctccggggc 
      gagttcccg tgccgtctc tgcacggcg gttttcccg 
      aatctgagg gccttaaaa gggcacaat agggcccat 
      cttaatggt aataacaat gggatgtat cgctgcaac 
      atgtgccca cactcatcc atcgataaa gcggcgctg 
      atcagacac atgaggacc cataatggg gacagaccg 
      tacgaatgc tctctctgt aactacgct ttcaccacc 
      aaggccaat tgtgagcgg cacttgagg aacagacac 
      gccaagaca acacgtgag gaggtcaag aaagctata 
      atctatcac ccgtcggaa gaccccacc aatgaggat 
      ttaaacaaa ctggcggaa ccacgcaaa agtgacttg 
      gtaccaacc cctgagaaa cacaaacct gagatagcg 
      cccctaatt cccacttta ctcaaggag acactcaat 
      gtccccctc cctaaggaa cctcccctt ttctcaaac 
      atcatccgg agcgagaat ttgaaaagt ttgaaggaa 
      cacaggact aatgggcaa ttttcgccc tatttgccg 
      ccttacaac ttcacacca aaacctaca aacgtctca 
      accccccat tcaaaaatc caggtcaag aacctggag 
      gagctgaag gagccctcc gacttggag gaggagcag 
      gaggaggaa ccaatgaac ttggtcctt gatttgagc 
      aagaagaaa gaggtcaag ccggaagta ccgcaagac 
      ctgtcaaag aaaaccccc ccgttgcaa atgcgcgat 
      ttattcgcc caacagtta ctcaaaact ccgccaaaa 
      atcgaccct gcaactctc tacgcctcg atttaccgc 
      aacaccttc ccgggattc cccggctgg cctttaaac 
      ccgctcctg ttttcctcc ctccagacc ccggtactc 
      cccccggag tcggacatg aaggagcgc ttgcagcgc 
      ttccagttc tgcggcggc tccatgatc agtgacaac 
      ttcaggatg caaaccccc atcgaactg aagaaaacc 
      gagcctttg agtctcaac gtggaggcg aactttggc 
      gacaactac aagttgcaa agtccggtc gcgaagccc 
      gacctcacc cagtcgccc aattcggtc aagatggtg 
      atcaagaac ggggttttg atgccgaag cagaaacag 
      aggcggtat cggaccgaa cgtcccttc acctgcgag 
      cattgctcg gctaggttt acgttaagg tcgaacatg 
      gagagacac atcaagcag cagcatccc cagttttgg 
      tcgcagcgc cagagaggc gtgagcaac ccggggcgc 
      aaaagccag atgaagagt tactgtgat ctcagtata 
      ccaaactac gaagtacca aagagtcag gattatgac 
      gataccaaa gaacacatg gaggagaag ttgaagttt 
      gcgattctg gcgcagcat ttgagggtc gggcaggag 
      gtgaagaag gacgatgat gaggatggg gcgcttgtg 
      atcgacgag aatgccgag aaggccgaa aagactgag 
      gatagtagg tgttacgag acggggttt gaacggagt 
      aagattctc gaggagaag ttgctagag ttgcgtgcg 
      aagcagatc aagtcggag aaggtgaag aatgaggag 
      gatttggtg ccggtgtcc aggcttctg gataatgcg 
      tcacaacaa cagttcaag gagtatttc aagagagag 
      ggcgaggag ccggaggtt ggaggagcg agtgaggag 
      gatgaggaa gggctagtg gccagtggg agcacgagc 
      gaagggaac atctcagga acggacgaa aataaatcc 
      gagtcggag accgccgcc cccgccaag aagaagtcc 
      gcctactcc atggccccc aaccgcgtg agctgcccc 
      ttctgcagc cgcaaattc ccgtggagc tcttcgttg 
      cgccgccac atcctcacc cacaccggc cagaagccg 
      ttcaaatgc agccactgt cctctcctc ttcaccacc 
      aagtccaac tgcgaccgc cacttgctg cgcaaacac 
      ggcaacaac gccaccacc atcaccaac gacgccgtc 
      aacaacaac accaactat ctgatgcgc aacgtgccc 
      gaacgcccc ttcaagtgc tccaactgc cccagcagc 
      accttctcc acctactcc aacctgaag aagcacatc 
      agctgcaag cactccggc gacgacatc aaggcccaa 
      ggctacgaa gccggcagt tccgaggac gagaaactg 
      ccaacaaac gagtccaaa accgactgg gacgagggc 
      aaaaaatcc gaagtccag aataacgtg gtgcagaat 
      tccgatcta ccgttcaag tgccatttg tgcgatggc 
      tcgtttggg gagcgtcaa gatgcattg gaccacatc 
      aaggacaag cattacgca gagtacgac ctgctaatg 
      tccaaaaat gcgctagat aacaacgcc acaaccccc 
      gaagaaggg ggccaccat gaggacgag gagaacgac 
      atgaggggg aaattcccg gattattcc aacaggaag 
      gtgatttgc gccttctgc atgcgtcgc ttctggtcg 
      gcggaggat ctcagaagg cacatgagg acccacacg 
      ggggagcgc cccttcagc tgtgacata tgccggcgc 
      agattcacc ctcaagcat agcatgttg cgccaccga 
      aagaaacac aacgtgaac ttcgacagc gaaatcgtc 
      ggaagcgac gacgataac aacgcccca aatttcgca 
      aaaacggaa aataacaac acccagaaa gaggagtcg 
      gacgggaac gagggtagc gacttgatt agtaagtta 
      ttagggatc agggatagg tctataatt gataaggtt 
      ctgacggcg tctgccgac gacgccgcc aagctccta 
      ggagtcaaa aacggggag aaagaatag tctagtgtg 
      cttgtgtag attatttat ttattttgt aaattttgt 
      accttttta ggcttgtaa acactgcgt ctagtcagg 
      ttatgtaaa aacgaagca aatgagtat agactagtg 
      atgatgtgt ataaaactt cagttgtaa gcgtaaatt 
      aattaatat atttttgta cataacaaa tatgatacg 
      gtctagaat accaaataa tcaggttat aaccggcgg 
      ttttgcaaa aaccggcgt tgggttttg acttcgaat 
      aatgtgtct taatattgc cttagtatt aagtcactg 
      taaagtaag caatatgtt tgtatggag cgcacccgc 
      caagctggg gcttgattt tgtacggat agaacgaca 
      cgtttttat ttcttgttg cacttattg attagtatt 
      gtattattc acattcacg ctgtgtctt aaaagtatt 
      tacggcttg cggtgcgat gggacgtcg cactcacgg 
      agcatttta gcaatttga aagcaatga gagtgaatt 
      agtgttatt acagatttg atgctttaa aaatgtctt 
      tattgtaat acgttctcg ttttccatc gattttagg 
      tatgccata ttcctgtag agtctaatg tacttaagt 
      tagtgtaaa atgacatat tatacctat tatatttgt 
      ttgaataaa ccttcattt caata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY