| General information | |||
| TC009560 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2544508..2562187 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC009560 | |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_08638 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
maxilla bristle position ventralized - Penetrance (percentage of animals/eggs on slide): more than 80%
head cuticle lateral ventralconstricted - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen tracheal opening present - Penetrance (percentage of animals/eggs on slide): more than 80%
| Comment: Tracheal system disrupted, but tracheal openings not affected |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009560 RA
MQGVLFMPQ NPQQGSPPP NNALVTDIT MEVEPESTK QSPTNQSTQ SDSGIFSAT NTTATMDPC DTDNRSDCS SPERKYVCP ICDTMLTSQ HEFTLHIRS HNNETEAQD DKGFTCRIC FKVLSSSSS LDRHVLVHS GERPFHCRY CGDTFTTNG NMHRHMRTH SHKADNYES DASTDSGSS KSTEFNNNK VESKKRKLE ASDHANKRT ENASDCESQ FYKCPVCER TDFDSIVAL EAHLEDNHP DYPAKCGQC NQLFQNNKL LTIHRNTVH SSGNKRHTV AGFKDLTFV DFSSEKFPY IARYECEKN LHTASDGLT FQCKTCTRA FPCSSSLEI HQKDCQNQY NGLDLTKTQ VSDKEIRRN EFFARLNLQ DNSPEKFVP PPQISETST KLRQQLLAK VMTNDSKDL ADIQSIISM TTNGSLLQQ LQTKSDLAQ QETQRSEQE EESQDLFAV EFRKMKLRG EFPCRLCTA VFPNLRALK GHNRAHLNG NNNGMYRCN MCPHSSIDK AALIRHMRT HNGDRPYEC SLCNYAFTT KANCERHLR NRHAKTTRE EVKKAIIYH PSEDPTNED LNKLAEPRK SDLVPTPEK HKPEIAPLI PTLLKETLN VPLPKEPPL FSNIIRSEN LKSLKEHRT NGQFSPYLP PYNFTPKPT NVSTPHSKI QVKNLEELK EPSDLEEEQ EEEPMNLVL DLSKKKEVK PEVPQDLSK KTPPLQMRD LFAQQLLKT PPKIDPATL YASIYRNTF PGFPGWPLN PLLFSSLQT PVLPPESDM KERLQRFQF CGGSMISDN FRMQTPIEL KKTEPLSLN VEANFGDNY KLQSPVAKP DLTQSPNSV KMVIKNGVL MPKQKQRRY RTERPFTCE HCSARFTLR SNMERHIKQ QHPQFWSQR QRGVSNPGR KSQMKSYCD LSIPNYEVP KSQDYDDTK EHMEEKLKF AILAQHLRV GQEVKKDDD EDGALVIDE NAEKAEKTE DSRCYETGF ERSKILEEK LLELRAKQI KSEKVKNEE DLVPVSRLL DNASQQQFK EYFKREGEE PEVGGASEE DEEGLVASG STSEGNISG TDENKSESE TAAPAKKKS AYSMAPNRV SCPFCSRKF PWSSSLRRH ILTHTGQKP FKCSHCPLL FTTKSNCDR HLLRKHGNN ATTITNDAV NNNTNYLMR NVPERPFKC SNCPSSTFS TYSNLKKHI SCKHSGDDI KAQGYEAGS SEDEKLPTN ESKTDWDEG KKSEVQNNV VQNSDLPFK CHLCDGSFG ERQDALDHI KDKHYAEYD LLMSKNALD NNATTPEEG GHHEDEEND MRGKFPDYS NRKVICAFC MRRFWSAED LRRHMRTHT GERPFSCDI CRRRFTLKH SMLRHRKKH NVNFDSEIV GSDDDNNAP NFAKTENNN TQKEESDGN EGSDLISKL LGIRDRSII DKVLTASAD DAAKLLGVK NGEKE
- >TC009560 RA
atgcaaggg gtgctcttc atgccgcag aatccacag cagggctcg ccgccgcca aacaacgct ctagtaacc gacattacc atggaggta gaacccgaa tcaacgaaa caatcccca acgaaccaa agcacccaa agcgactct ggcatcttc tccgccacc aacaccacc gccaccatg gacccctgc gacaccgac aaccgcagc gactgcagc tcccccgag cgcaaatac gtctgcccg atctgcgac accatgctg acctcccag cacgagttc accctccat atccgatct cacaacaac gaaacggag gcccaggac gacaagggc ttcacctgt cgcatttgc ttcaaagtg ctaagttcc agctcgagc ctggaccgc cacgtgctg gtccacagc ggcgaacgc cccttccat tgccgctac tgcggcgac accttcacc accaacgga aacatgcac cgacacatg cggacacat tcccacaaa gcggacaac tacgaatcc gacgccagc accgacagc ggaagctcc aaaagcacc gaattcaac aacaacaaa gtcgaatcg aaaaaacgc aagctggag gcgagcgac cacgcgaat aagcgaacg gagaacgcg agcgattgc gaaagccag ttctacaag tgcccggtt tgcgaacgc accgacttt gactcgatt gtggcgctg gaagcgcac ctggaggac aatcacccg gattatccc gccaaatgt gggcaatgc aaccaactc ttccaaaac aacaaactc ctaactatc catcggaac actgtacat tccagcggg aataaaaga cacacagtt gccgggttc aaagacttg acttttgtc gacttttcc agcgagaag tttccatat atcgcgcgg tacgaatgc gagaaaaac ctacacacg gcttccgac ggactcact ttccagtgc aagacctgc acacgggcg tttccctgc tccagctcg ctggaaatc caccagaaa gactgccag aaccaatac aatgggctt gacttgacc aagacgcaa gtctccgac aaggaaatc cggcggaac gagtttttc gccagattg aaccttcag gacaattcg ccggaaaag ttcgtccct ccgccacag atcagcgaa acttcgacc aaactccga cagcaactt ttggcgaaa gttatgacc aatgactcg aaggatctg gcggacatc cagtccatt atttcgatg actacgaac ggtagtctc ttgcaacaa ctccagacc aagagcgat ttggcgcaa caagagact caaaggtcg gaacaagag gaggaatct caggatttg ttcgccgtg gagtttcgc aaaatgaag ctccggggc gagttcccg tgccgtctc tgcacggcg gttttcccg aatctgagg gccttaaaa gggcacaat agggcccat cttaatggt aataacaat gggatgtat cgctgcaac atgtgccca cactcatcc atcgataaa gcggcgctg atcagacac atgaggacc cataatggg gacagaccg tacgaatgc tctctctgt aactacgct ttcaccacc aaggccaat tgtgagcgg cacttgagg aacagacac gccaagaca acacgtgag gaggtcaag aaagctata atctatcac ccgtcggaa gaccccacc aatgaggat ttaaacaaa ctggcggaa ccacgcaaa agtgacttg gtaccaacc cctgagaaa cacaaacct gagatagcg cccctaatt cccacttta ctcaaggag acactcaat gtccccctc cctaaggaa cctcccctt ttctcaaac atcatccgg agcgagaat ttgaaaagt ttgaaggaa cacaggact aatgggcaa ttttcgccc tatttgccg ccttacaac ttcacacca aaacctaca aacgtctca accccccat tcaaaaatc caggtcaag aacctggag gagctgaag gagccctcc gacttggag gaggagcag gaggaggaa ccaatgaac ttggtcctt gatttgagc aagaagaaa gaggtcaag ccggaagta ccgcaagac ctgtcaaag aaaaccccc ccgttgcaa atgcgcgat ttattcgcc caacagtta ctcaaaact ccgccaaaa atcgaccct gcaactctc tacgcctcg atttaccgc aacaccttc ccgggattc cccggctgg cctttaaac ccgctcctg ttttcctcc ctccagacc ccggtactc cccccggag tcggacatg aaggagcgc ttgcagcgc ttccagttc tgcggcggc tccatgatc agtgacaac ttcaggatg caaaccccc atcgaactg aagaaaacc gagcctttg agtctcaac gtggaggcg aactttggc gacaactac aagttgcaa agtccggtc gcgaagccc gacctcacc cagtcgccc aattcggtc aagatggtg atcaagaac ggggttttg atgccgaag cagaaacag aggcggtat cggaccgaa cgtcccttc acctgcgag cattgctcg gctaggttt acgttaagg tcgaacatg gagagacac atcaagcag cagcatccc cagttttgg tcgcagcgc cagagaggc gtgagcaac ccggggcgc aaaagccag atgaagagt tactgtgat ctcagtata ccaaactac gaagtacca aagagtcag gattatgac gataccaaa gaacacatg gaggagaag ttgaagttt gcgattctg gcgcagcat ttgagggtc gggcaggag gtgaagaag gacgatgat gaggatggg gcgcttgtg atcgacgag aatgccgag aaggccgaa aagactgag gatagtagg tgttacgag acggggttt gaacggagt aagattctc gaggagaag ttgctagag ttgcgtgcg aagcagatc aagtcggag aaggtgaag aatgaggag gatttggtg ccggtgtcc aggcttctg gataatgcg tcacaacaa cagttcaag gagtatttc aagagagag ggcgaggag ccggaggtt ggaggagcg agtgaggag gatgaggaa gggctagtg gccagtggg agcacgagc gaagggaac atctcagga acggacgaa aataaatcc gagtcggag accgccgcc cccgccaag aagaagtcc gcctactcc atggccccc aaccgcgtg agctgcccc ttctgcagc cgcaaattc ccgtggagc tcttcgttg cgccgccac atcctcacc cacaccggc cagaagccg ttcaaatgc agccactgt cctctcctc ttcaccacc aagtccaac tgcgaccgc cacttgctg cgcaaacac ggcaacaac gccaccacc atcaccaac gacgccgtc aacaacaac accaactat ctgatgcgc aacgtgccc gaacgcccc ttcaagtgc tccaactgc cccagcagc accttctcc acctactcc aacctgaag aagcacatc agctgcaag cactccggc gacgacatc aaggcccaa ggctacgaa gccggcagt tccgaggac gagaaactg ccaacaaac gagtccaaa accgactgg gacgagggc aaaaaatcc gaagtccag aataacgtg gtgcagaat tccgatcta ccgttcaag tgccatttg tgcgatggc tcgtttggg gagcgtcaa gatgcattg gaccacatc aaggacaag cattacgca gagtacgac ctgctaatg tccaaaaat gcgctagat aacaacgcc acaaccccc gaagaaggg ggccaccat gaggacgag gagaacgac atgaggggg aaattcccg gattattcc aacaggaag gtgatttgc gccttctgc atgcgtcgc ttctggtcg gcggaggat ctcagaagg cacatgagg acccacacg ggggagcgc cccttcagc tgtgacata tgccggcgc agattcacc ctcaagcat agcatgttg cgccaccga aagaaacac aacgtgaac ttcgacagc gaaatcgtc ggaagcgac gacgataac aacgcccca aatttcgca aaaacggaa aataacaac acccagaaa gaggagtcg gacgggaac gagggtagc gacttgatt agtaagtta ttagggatc agggatagg tctataatt gataaggtt ctgacggcg tctgccgac gacgccgcc aagctccta ggagtcaaa aacggggag aaagaatag
- >TC009560 RA
ccgctcacc cacagccag gcgacttga tctttttaa gctcacttc gcgttccat gtcttgctc gcgagcatc acatcgaga gtgctggtt gatgagttc gaatttgat gacttgtga cgatcaatt gggacaaaa tcggtgcgg atcagtggc gtggtgcac ttgtgatgg attaccttt ccccaactc cgagaacgt aatttcgaa atgcaaggg gtgctcttc atgccgcag aatccacag cagggctcg ccgccgcca aacaacgct ctagtaacc gacattacc atggaggta gaacccgaa tcaacgaaa caatcccca acgaaccaa agcacccaa agcgactct ggcatcttc tccgccacc aacaccacc gccaccatg gacccctgc gacaccgac aaccgcagc gactgcagc tcccccgag cgcaaatac gtctgcccg atctgcgac accatgctg acctcccag cacgagttc accctccat atccgatct cacaacaac gaaacggag gcccaggac gacaagggc ttcacctgt cgcatttgc ttcaaagtg ctaagttcc agctcgagc ctggaccgc cacgtgctg gtccacagc ggcgaacgc cccttccat tgccgctac tgcggcgac accttcacc accaacgga aacatgcac cgacacatg cggacacat tcccacaaa gcggacaac tacgaatcc gacgccagc accgacagc ggaagctcc aaaagcacc gaattcaac aacaacaaa gtcgaatcg aaaaaacgc aagctggag gcgagcgac cacgcgaat aagcgaacg gagaacgcg agcgattgc gaaagccag ttctacaag tgcccggtt tgcgaacgc accgacttt gactcgatt gtggcgctg gaagcgcac ctggaggac aatcacccg gattatccc gccaaatgt gggcaatgc aaccaactc ttccaaaac aacaaactc ctaactatc catcggaac actgtacat tccagcggg aataaaaga cacacagtt gccgggttc aaagacttg acttttgtc gacttttcc agcgagaag tttccatat atcgcgcgg tacgaatgc gagaaaaac ctacacacg gcttccgac ggactcact ttccagtgc aagacctgc acacgggcg tttccctgc tccagctcg ctggaaatc caccagaaa gactgccag aaccaatac aatgggctt gacttgacc aagacgcaa gtctccgac aaggaaatc cggcggaac gagtttttc gccagattg aaccttcag gacaattcg ccggaaaag ttcgtccct ccgccacag atcagcgaa acttcgacc aaactccga cagcaactt ttggcgaaa gttatgacc aatgactcg aaggatctg gcggacatc cagtccatt atttcgatg actacgaac ggtagtctc ttgcaacaa ctccagacc aagagcgat ttggcgcaa caagagact caaaggtcg gaacaagag gaggaatct caggatttg ttcgccgtg gagtttcgc aaaatgaag ctccggggc gagttcccg tgccgtctc tgcacggcg gttttcccg aatctgagg gccttaaaa gggcacaat agggcccat cttaatggt aataacaat gggatgtat cgctgcaac atgtgccca cactcatcc atcgataaa gcggcgctg atcagacac atgaggacc cataatggg gacagaccg tacgaatgc tctctctgt aactacgct ttcaccacc aaggccaat tgtgagcgg cacttgagg aacagacac gccaagaca acacgtgag gaggtcaag aaagctata atctatcac ccgtcggaa gaccccacc aatgaggat ttaaacaaa ctggcggaa ccacgcaaa agtgacttg gtaccaacc cctgagaaa cacaaacct gagatagcg cccctaatt cccacttta ctcaaggag acactcaat gtccccctc cctaaggaa cctcccctt ttctcaaac atcatccgg agcgagaat ttgaaaagt ttgaaggaa cacaggact aatgggcaa ttttcgccc tatttgccg ccttacaac ttcacacca aaacctaca aacgtctca accccccat tcaaaaatc caggtcaag aacctggag gagctgaag gagccctcc gacttggag gaggagcag gaggaggaa ccaatgaac ttggtcctt gatttgagc aagaagaaa gaggtcaag ccggaagta ccgcaagac ctgtcaaag aaaaccccc ccgttgcaa atgcgcgat ttattcgcc caacagtta ctcaaaact ccgccaaaa atcgaccct gcaactctc tacgcctcg atttaccgc aacaccttc ccgggattc cccggctgg cctttaaac ccgctcctg ttttcctcc ctccagacc ccggtactc cccccggag tcggacatg aaggagcgc ttgcagcgc ttccagttc tgcggcggc tccatgatc agtgacaac ttcaggatg caaaccccc atcgaactg aagaaaacc gagcctttg agtctcaac gtggaggcg aactttggc gacaactac aagttgcaa agtccggtc gcgaagccc gacctcacc cagtcgccc aattcggtc aagatggtg atcaagaac ggggttttg atgccgaag cagaaacag aggcggtat cggaccgaa cgtcccttc acctgcgag cattgctcg gctaggttt acgttaagg tcgaacatg gagagacac atcaagcag cagcatccc cagttttgg tcgcagcgc cagagaggc gtgagcaac ccggggcgc aaaagccag atgaagagt tactgtgat ctcagtata ccaaactac gaagtacca aagagtcag gattatgac gataccaaa gaacacatg gaggagaag ttgaagttt gcgattctg gcgcagcat ttgagggtc gggcaggag gtgaagaag gacgatgat gaggatggg gcgcttgtg atcgacgag aatgccgag aaggccgaa aagactgag gatagtagg tgttacgag acggggttt gaacggagt aagattctc gaggagaag ttgctagag ttgcgtgcg aagcagatc aagtcggag aaggtgaag aatgaggag gatttggtg ccggtgtcc aggcttctg gataatgcg tcacaacaa cagttcaag gagtatttc aagagagag ggcgaggag ccggaggtt ggaggagcg agtgaggag gatgaggaa gggctagtg gccagtggg agcacgagc gaagggaac atctcagga acggacgaa aataaatcc gagtcggag accgccgcc cccgccaag aagaagtcc gcctactcc atggccccc aaccgcgtg agctgcccc ttctgcagc cgcaaattc ccgtggagc tcttcgttg cgccgccac atcctcacc cacaccggc cagaagccg ttcaaatgc agccactgt cctctcctc ttcaccacc aagtccaac tgcgaccgc cacttgctg cgcaaacac ggcaacaac gccaccacc atcaccaac gacgccgtc aacaacaac accaactat ctgatgcgc aacgtgccc gaacgcccc ttcaagtgc tccaactgc cccagcagc accttctcc acctactcc aacctgaag aagcacatc agctgcaag cactccggc gacgacatc aaggcccaa ggctacgaa gccggcagt tccgaggac gagaaactg ccaacaaac gagtccaaa accgactgg gacgagggc aaaaaatcc gaagtccag aataacgtg gtgcagaat tccgatcta ccgttcaag tgccatttg tgcgatggc tcgtttggg gagcgtcaa gatgcattg gaccacatc aaggacaag cattacgca gagtacgac ctgctaatg tccaaaaat gcgctagat aacaacgcc acaaccccc gaagaaggg ggccaccat gaggacgag gagaacgac atgaggggg aaattcccg gattattcc aacaggaag gtgatttgc gccttctgc atgcgtcgc ttctggtcg gcggaggat ctcagaagg cacatgagg acccacacg ggggagcgc cccttcagc tgtgacata tgccggcgc agattcacc ctcaagcat agcatgttg cgccaccga aagaaacac aacgtgaac ttcgacagc gaaatcgtc ggaagcgac gacgataac aacgcccca aatttcgca aaaacggaa aataacaac acccagaaa gaggagtcg gacgggaac gagggtagc gacttgatt agtaagtta ttagggatc agggatagg tctataatt gataaggtt ctgacggcg tctgccgac gacgccgcc aagctccta ggagtcaaa aacggggag aaagaatag tctagtgtg cttgtgtag attatttat ttattttgt aaattttgt accttttta ggcttgtaa acactgcgt ctagtcagg ttatgtaaa aacgaagca aatgagtat agactagtg atgatgtgt ataaaactt cagttgtaa gcgtaaatt aattaatat atttttgta cataacaaa tatgatacg gtctagaat accaaataa tcaggttat aaccggcgg ttttgcaaa aaccggcgt tgggttttg acttcgaat aatgtgtct taatattgc cttagtatt aagtcactg taaagtaag caatatgtt tgtatggag cgcacccgc caagctggg gcttgattt tgtacggat agaacgaca cgtttttat ttcttgttg cacttattg attagtatt gtattattc acattcacg ctgtgtctt aaaagtatt tacggcttg cggtgcgat gggacgtcg cactcacgg agcatttta gcaatttga aagcaatga gagtgaatt agtgttatt acagatttg atgctttaa aaatgtctt tattgtaat acgttctcg ttttccatc gattttagg tatgccata ttcctgtag agtctaatg tacttaagt tagtgtaaa atgacatat tatacctat tatatttgt ttgaataaa ccttcattt caata
