Status
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Gene information
General information
TC009544 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2246447..2250221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009544
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08634

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 abdomen musculature random rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009544 RA
      MSSNYNIEW KTCTDEHFS AKTKGLSGV KTTDHPLKT 
      AIVTERVPR RILGSQPST GRTTPVSTP SVILEPLSM 
      ALEGVDPLT AFAIEEMDP LTKLAAQES RDKVDSSQD 
      KKLSDYKCE VDAWAMKKA SILSKYTTS EKISIVTSF 
      LSDGDKVVV KAQSTAVDK MQHRLEQLD YIEEGSQTR 
      LDLSQTEYV NRIEQLNRE LVSAWNSEQ RVKALKIAI 
      QCAKLLSDT SVLTFYPSK FVLITDILD VFGQLVYNR 
      LSTKTDSHK LGSKTKLPE DFTPEMVSD SAKETCLNW 
      FYKIASIRE LVPRLYVEM ALMKSYYFL SLSECQEAL 
      RRLTHMING IGNPLVAVY ARCYLCRVG CSLSNRIKN 
      KEYLLQNFH RFLQNYQHL FSRSVKGEL AQQKVSQSV 
      YMSLFTPAL DYMLQTVAC DNSDALLNE LLERCKVHG 
      NSSLILNTV MSAFKPHLI SARTLQFLE MIEHCLDQG 
      IPLHSLLRT LGLCVSVAP PPLEHRRQI LSTAWKHIT 
      GLKQVEEYI ACAEPWVLY VVKYFSHRE LNTILGDII 
      DHIAPDRSY EQYYNQLKN VVENIVLNI QDFEALLVM 
      DNFLPLIDL FQQESIRVE VCKKVLSGS NSNLIFVND 
      AVVVNALMF LCTVLHDSV NALTPEDEY RQIGDILCH 
      VIKTIDYGR DFEQQLTFY VEARASFSN IDIVFVQLV 
      QCVNALSVK TRQIVKGLH TRKTGDFVR ACAAYCFIT 
      VPSIKSAKH RLRLYLLSG QVALFNHCL GQADACFKA 
      ALTTISEIQ NDKTQFPES ELIPYVKQF LSILLVVPD 
      NPDCSVLNL TRALLNVLQ GYSWESNIS LISIYLSVI 
      DMLSVMAQE WYPYHIDKV ESNDSLYGS DKKFIAEIN 
      KICSVVTNE LMSKLQSLG PCTKQSTFA IELFVKLAV 
      RNDLTNQML VLALNLWNL AKRDNTLDT KYLVCETLD 
      ENNVSILCI LPDQNKKLR KKTNVRMQ
    CDS sequence
    • >TC009544 RA
      atgagtagc aattacaac atagaatgg aaaacatgc 
      acagacgag cacttttct gcaaaaaca aaaggactc 
      agtggggtc aagaccacc gatcacccc cttaaaacg 
      gcaattgtg actgagcgc gttccacgt cggattttg 
      gggtcgcag cccagcacc ggtcgcacg acccccgtg 
      tccaccccc tctgtgatt ttggagcct ttgagtatg 
      gcccttgaa ggggttgac cccttgacg gccttcgca 
      atcgaggaa atggaccct ttgacaaaa cttgcagca 
      caagagagc agggacaag gttgattca agtcaagat 
      aagaaatta tcggattat aagtgcgaa gtcgacgcg 
      tgggctatg aaaaaagcg tcgattttg tccaaatat 
      acaacgtcc gagaagatt tcaattgtt acaagtttt 
      ctgtcagat ggggataaa gttgtcgtc aaggcgcaa 
      agtactgct gttgataaa atgcaacac aggcttgaa 
      cagctggat tacatagaa gaggggtca caaaccagg 
      cttgatctg tcacaaact gagtatgtg aacaggatt 
      gaacaactc aaccgggaa cttgtctca gcttggaat 
      tcggaacaa agggtcaaa gcattaaaa attgcaata 
      cagtgtgct aaactatta tcagatact agcgttttg 
      acgttttat cctagcaaa tttgtactc attacggat 
      attttggac gtttttgga caattggtt tacaataga 
      ttgagtacg aaaacagat tcacacaag ttgggttca 
      aaaacgaaa ttaccggag gatttcacc cctgaaatg 
      gtctcagat tcggcaaaa gaaacatgt ttgaactgg 
      ttttacaaa atcgcatcg attcgcgaa ctagtcccg 
      aggctatac gtcgagatg gcgctcatg aagtcgtac 
      tatttcctc tccctgagt gagtgccaa gaggcttta 
      aggcgcctc acacatatg attaacggc attggcaac 
      cctcttgtg gcggtttac gcccgttgt tacctttgt 
      agagtcggt tgcagtttg tccaaccgt ataaaaaac 
      aaggaatat ctcttacaa aatttccac cggtttttg 
      caaaactac caacacttg tttagtcgc agcgttaag 
      ggcgaatta gcccaacag aaagtctca caatcggtt 
      tacatgtct ttgttcacc ccggccctt gattatatg 
      ctgcaaaca gtggcgtgc gacaattcc gatgcactc 
      ttgaacgag ctcctcgag aggtgcaaa gtgcacggg 
      aatagttcg ttgattctt aacacggtc atgtcggcc 
      ttcaagccg catttaata tcagcccga actttgcaa 
      tttttggaa atgatcgag cattgctta gatcaggga 
      attccccta cattccctt ttaagaacg ttgggattg 
      tgtgtgagt gtggcgccc cctccgttg gaacatcga 
      agacagatt ttaagcacg gcttggaaa cacataacg 
      ggcttaaaa caagtcgag gagtatata gcatgcgct 
      gagccttgg gtactttac gttgtcaaa tacttctcg 
      catcgcgaa ttaaacact atactgggc gacataatc 
      gaccacata gcgccggat cgtagctac gaacaatat 
      tacaatcaa ctaaaaaac gttgttgag aacatagtt 
      ttaaatatc caagatttt gaggccctc cttgtcatg 
      gacaatttc ctgccgctg atcgacttg ttccagcaa 
      gaatcaata agggtggaa gtgtgcaaa aaagtgcta 
      agtggaagc aacagcaac cttattttt gtcaacgac 
      gcggtcgtt gtcaacgcc ctcatgttc ctctgtact 
      gttctacac gactctgtt aacgcgcta acccccgaa 
      gatgagtac cgacaaatt ggcgacatt ttatgccat 
      gtgatcaaa acaattgac tacggacgt gactttgaa 
      caacaattg acattctac gttgaggct cgtgccagt 
      ttttccaat attgacata gttttcgtg caattggta 
      caatgtgtt aacgcccta tctgtcaaa acacgccaa 
      atcgtcaaa gggctacac acgagaaaa acaggcgat 
      ttcgtgcgc gcatgcgcc gcttattgc ttcattact 
      gtaccttcc atcaagtcg gccaagcac cgcttgcgg 
      ttgtacctg ctttcggga caggtggcg ctgtttaac 
      cattgtttg ggccaagcg gatgcgtgt ttcaaggcc 
      gccttgacc actatttcg gagatacaa aatgacaaa 
      acgcaattt cccgaaagc gaactcatt ccgtacgtt 
      aaacagttt ttgtcgatt ttgttggta gtgcccgat 
      aatcccgac tgtagtgtt cttaacttg acgagggcg 
      cttttgaat gtgctacaa gggtacagt tgggagtcg 
      aatatttcg ctcatttcg atttatttg agtgtcatt 
      gacatgttg agtgtgatg gcgcaagag tggtacccc 
      taccacatc gataaagtt gaatcaaac gattctttg 
      tacggttct gataagaaa tttatcgcc gaaattaat 
      aaaatctgt tcagttgtg accaacgaa ttgatgagt 
      aaattacag tcgttggga ccgtgcacc aaacagtcg 
      acttttgcc atcgaattg ttcgtaaag ttggccgtg 
      aggaacgac ctgactaat caaatgcta gttctggcg 
      ctaaattta tggaactta gccaaacgt gacaacact 
      ttggacacg aaatatctg gtatgtgaa acacttgat 
      gaaaataat gtaagtata ttgtgtatt cttccagat 
      cagaacaaa aaattacgt aaaaaaacg aacgttcgc 
      atgcaataa
    mRNA sequence
    • >TC009544 RA
      acccactct tgtttcaat ttttttagt caactttta 
      tttacttga gttaatttg tgcgataat ctttagtgg 
      tctctccaa acgagccgc cactggcca attgctgtt 
      ttggtaaac tgacacttg gcaacaatt gttgtttct 
      ctatccttg tcagtggca cacgcacgg aaaatagtc 
      atctctttc taatcctta acttcaagt tttaggtta 
      ggataactg tctcttggc aataaagtt gtaaaatta 
      agaaaaaca tgagtagca attacaaca tagaatgga 
      aaacatgca cagacgagc acttttctg caaaaacaa 
      aaggactca gtggggtca agaccaccg atcaccccc 
      ttaaaacgg caattgtga ctgagcgcg ttccacgtc 
      ggattttgg ggtcgcagc ccagcaccg gtcgcacga 
      cccccgtgt ccaccccct ctgtgattt tggagcctt 
      tgagtatgg cccttgaag gggttgacc ccttgacgg 
      ccttcgcaa tcgaggaaa tggaccctt tgacaaaac 
      ttgcagcac aagagagca gggacaagg ttgattcaa 
      gtcaagata agaaattat cggattata agtgcgaag 
      tcgacgcgt gggctatga aaaaagcgt cgattttgt 
      ccaaatata caacgtccg agaagattt caattgtta 
      caagttttc tgtcagatg gggataaag ttgtcgtca 
      aggcgcaaa gtactgctg ttgataaaa tgcaacaca 
      ggcttgaac agctggatt acatagaag aggggtcac 
      aaaccaggc ttgatctgt cacaaactg agtatgtga 
      acaggattg aacaactca accgggaac ttgtctcag 
      cttggaatt cggaacaaa gggtcaaag cattaaaaa 
      ttgcaatac agtgtgcta aactattat cagatacta 
      gcgttttga cgttttatc ctagcaaat ttgtactca 
      ttacggata ttttggacg tttttggac aattggttt 
      acaatagat tgagtacga aaacagatt cacacaagt 
      tgggttcaa aaacgaaat taccggagg atttcaccc 
      ctgaaatgg tctcagatt cggcaaaag aaacatgtt 
      tgaactggt tttacaaaa tcgcatcga ttcgcgaac 
      tagtcccga ggctatacg tcgagatgg cgctcatga 
      agtcgtact atttcctct ccctgagtg agtgccaag 
      aggctttaa ggcgcctca cacatatga ttaacggca 
      ttggcaacc ctcttgtgg cggtttacg cccgttgtt 
      acctttgta gagtcggtt gcagtttgt ccaaccgta 
      taaaaaaca aggaatatc tcttacaaa atttccacc 
      ggtttttgc aaaactacc aacacttgt ttagtcgca 
      gcgttaagg gcgaattag cccaacaga aagtctcac 
      aatcggttt acatgtctt tgttcaccc cggcccttg 
      attatatgc tgcaaacag tggcgtgcg acaattccg 
      atgcactct tgaacgagc tcctcgaga ggtgcaaag 
      tgcacggga atagttcgt tgattctta acacggtca 
      tgtcggcct tcaagccgc atttaatat cagcccgaa 
      ctttgcaat ttttggaaa tgatcgagc attgcttag 
      atcagggaa ttcccctac attcccttt taagaacgt 
      tgggattgt gtgtgagtg tggcgcccc ctccgttgg 
      aacatcgaa gacagattt taagcacgg cttggaaac 
      acataacgg gcttaaaac aagtcgagg agtatatag 
      catgcgctg agccttggg tactttacg ttgtcaaat 
      acttctcgc atcgcgaat taaacacta tactgggcg 
      acataatcg accacatag cgccggatc gtagctacg 
      aacaatatt acaatcaac taaaaaacg ttgttgaga 
      acatagttt taaatatcc aagattttg aggccctcc 
      ttgtcatgg acaatttcc tgccgctga tcgacttgt 
      tccagcaag aatcaataa gggtggaag tgtgcaaaa 
      aagtgctaa gtggaagca acagcaacc ttatttttg 
      tcaacgacg cggtcgttg tcaacgccc tcatgttcc 
      tctgtactg ttctacacg actctgtta acgcgctaa 
      cccccgaag atgagtacc gacaaattg gcgacattt 
      tatgccatg tgatcaaaa caattgact acggacgtg 
      actttgaac aacaattga cattctacg ttgaggctc 
      gtgccagtt tttccaata ttgacatag ttttcgtgc 
      aattggtac aatgtgtta acgccctat ctgtcaaaa 
      cacgccaaa tcgtcaaag ggctacaca cgagaaaaa 
      caggcgatt tcgtgcgcg catgcgccg cttattgct 
      tcattactg taccttcca tcaagtcgg ccaagcacc 
      gcttgcggt tgtacctgc tttcgggac aggtggcgc 
      tgtttaacc attgtttgg gccaagcgg atgcgtgtt 
      tcaaggccg ccttgacca ctatttcgg agatacaaa 
      atgacaaaa cgcaatttc ccgaaagcg aactcattc 
      cgtacgtta aacagtttt tgtcgattt tgttggtag 
      tgcccgata atcccgact gtagtgttc ttaacttga 
      cgagggcgc ttttgaatg tgctacaag ggtacagtt 
      gggagtcga atatttcgc tcatttcga tttatttga 
      gtgtcattg acatgttga gtgtgatgg cgcaagagt 
      ggtacccct accacatcg ataaagttg aatcaaacg 
      attctttgt acggttctg ataagaaat ttatcgccg 
      aaattaata aaatctgtt cagttgtga ccaacgaat 
      tgatgagta aattacagt cgttgggac cgtgcacca 
      aacagtcga cttttgcca tcgaattgt tcgtaaagt 
      tggccgtga ggaacgacc tgactaatc aaatgctag 
      ttctggcgc taaatttat ggaacttag ccaaacgtg 
      acaacactt tggacacga aatatctgg tatgtgaaa 
      cacttgatg aaaataatg taagtatat tgtgtattc 
      ttccagatc agaacaaaa aattacgta aaaaaacga 
      acgttcgca tgcaataac acactgtta caagaacta 
      gtaaataaa atggaatga ggcag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY