General information | |||
TC009265 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 2196851..2202395 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC009265 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08632 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen dorsal partiallynot fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen posterior rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009265 RA
MDPSISWLQ PEQEGPAKH LWLRIWQTQ QELDSMNLK DVDSDPEFF PNGLSNHIM NGKTDKSGS TFVQRRRKG DNRASTKMM SRHPQKVVG EFGGCPWRK DPHVYARGI LGLHQEIDH FYQYMSPTR TEHLVRDDV VCRIKQIIL SKWPEAQVE VFGSYRTGL YLPTSDIDL VVIGKWSNL PLRTLEQEF LDNDVAQEN SIKVLDKAS VPIVKLTDK QTEIKVDIS FNMSNGVKS AELIKTYIH QFPVLPKLV YVLKQFLLE RDLNEVFTG GISSYSLIL MCISFLQLH PRPEMFVRG DANLGVFLI EFFELYGRK FNYMHTGIR IRDGGRYIS KEEMQKDMV DGHRPSLLC IEDPLLPSN DIGRSSYGV LQVKRAFEY AYTVLTNAV LPISSHNVE HSILGRIIR VTDKVIQYR RWIEKTFGH KYNSPTIPR PRPPRSLSS SGSTSSVEE SGGSSEGSD PPSRDISPN NMHVQRHIQ PKNHNQWRN NRKYKNSPH NNYNQSTTT KRKKQTKQY
- >TC009265 RA
atggatcct tcgatatct tggttgcaa ccggagcaa gagggtcct gccaaacat ttgtggctt cgaatttgg caaacacaa caggagctg gacagcatg aacttgaaa gacgtcgac agcgacccg gagttcttc cccaacggc ctctccaac cacatcatg aacggcaag acggacaag agcggctcc actttcgtt cagcggaga cgtaagggc gacaatcgc gccagcacc aagatgatg agccgccat ccgcagaag gtggtgggc gagttcggg ggctgtccg tggcgcaag gacccgcac gtctacgcc agaggcatc ctgggtctg catcaggaa atcgaccac ttttaccag tacatgagc cccacaagg accgaacac ctcgtcagg gatgacgtc gtctgtagg atcaaacaa atcattctc tccaaatgg ccagaagca caagttgaa gttttcgga tcttacagg accgggctc tatcttccg acaagcgac atagacttg gttgttata ggcaaatgg tcaaacctt ccccttcgg acgctggaa caggaattc ctagacaac gacgtggcg caagagaac agcataaaa gtcctcgac aaagcgtca gtgccgatc gtgaaatta accgacaaa cagaccgaa atcaaagtg gacatttcc ttcaatatg tcgaacggt gtaaaatcg gccgaactg ataaaaact tacatacat cagtttcca gtgttgccg aaattagtt tacgtttta aaacagttc cttttagaa agagactta aacgaagtg ttcaccggt ggtatatct agttacagt ttaatattg atgtgcata agtttcctt cagttgcat ccacgaccg gaaatgttt gtcagaggc gacgctaat ttaggcgtt ttccttatt gaatttttc gaattgtac ggtagaaaa tttaattat atgcatacc ggaattcgg atacgagac ggcggacgg tacattagc aaagaagaa atgcaaaag gacatggtg gacggacac aggcctagt cttttgtgc atcgaagat ccactcttg ccatccaac gacatcggc agaagcagc tacggcgtc ctgcaggtg aaacgggct ttcgagtac gcgtacact gttttaacg aatgccgtc cttccaatc tcgtcacac aacgtggag cactcgata ttaggtaga ataataaga gtaaccgac aaagtgatc caataccgg cgatggatc gaaaaaact tttggtcat aagtacaac tctccgacg atcccccga ccaaggcct cctcgttcc ctgtccagc agcggctcc acatcgagc gttgaggaa agtgggggc agttcagaa gggtccgat cctccttcg cgcgacata tcaccgaac aacatgcat gtccaacgc cacatccag ccaaagaat cacaatcaa tggagaaat aatcggaaa tataagaat agccctcac aataattat aaccagtcg accacaaca aagcgtaaa aaacaaacc aaacaatac tga
- >TC009265 RA
cgagttgaa atttgcgac agtggtcgc gaaactggt ttcgcttga atctggttt ggttcggtg ggtggttta aaaccgtgg gatggtttg gtggtcgct ggggcgctt agcaccgct cgtgttgga caagtgaca gcgcgagga gtgacgttt cggtggtgt ttgtgtgta tttttgtga gttttgggc gcttttttg aaattggcg ccacgtcag tcgggtgtc gggcgcgct atctaccga gcatgcgag acacctgtc agtttttgg tcgtgtgat atgcacaag gtgtgactt cagcgtgtg ttttaatta aaattgatg taataaata aaaatagcc gtatggtga agaaatcat aaactatct gtgattgtt tgaatggat ccttcgata tcttggttg caaccggag caagagggt cctgccaaa catttgtgg cttcgaatt tggcaaaca caacaggag ctggacagc atgaacttg aaagacgtc gacagcgac ccggagttc ttccccaac ggcctctcc aaccacatc atgaacggc aagacggac aagagcggc tccactttc gttcagcgg agacgtaag ggcgacaat cgcgccagc accaagatg atgagccgc catccgcag aaggtggtg ggcgagttc gggggctgt ccgtggcgc aaggacccg cacgtctac gccagaggc atcctgggt ctgcatcag gaaatcgac cacttttac cagtacatg agccccaca aggaccgaa cacctcgtc agggatgac gtcgtctgt aggatcaaa caaatcatt ctctccaaa tggccagaa gcacaagtt gaagttttc ggatcttac aggaccggg ctctatctt ccgacaagc gacatagac ttggttgtt ataggcaaa tggtcaaac cttcccctt cggacgctg gaacaggaa ttcctagac aacgacgtg gcgcaagag aacagcata aaagtcctc gacaaagcg tcagtgccg atcgtgaaa ttaaccgac aaacagacc gaaatcaaa gtggacatt tccttcaat atgtcgaac ggtgtaaaa tcggccgaa ctgataaaa acttacata catcagttt ccagtgttg ccgaaatta gtttacgtt ttaaaacag ttcctttta gaaagagac ttaaacgaa gtgttcacc ggtggtata tctagttac agtttaata ttgatgtgc ataagtttc cttcagttg catccacga ccggaaatg tttgtcaga ggcgacgct aatttaggc gttttcctt attgaattt ttcgaattg tacggtaga aaatttaat tatatgcat accggaatt cggatacga gacggcgga cggtacatt agcaaagaa gaaatgcaa aaggacatg gtggacgga cacaggcct agtcttttg tgcatcgaa gatccactc ttgccatcc aacgacatc ggcagaagc agctacggc gtcctgcag gtgaaacgg gctttcgag tacgcgtac actgtttta acgaatgcc gtccttcca atctcgtca cacaacgtg gagcactcg atattaggt agaataata agagtaacc gacaaagtg atccaatac cggcgatgg atcgaaaaa acttttggt cataagtac aactctccg acgatcccc cgaccaagg cctcctcgt tccctgtcc agcagcggc tccacatcg agcgttgag gaaagtggg ggcagttca gaagggtcc gatcctcct tcgcgcgac atatcaccg aacaacatg catgtccaa cgccacatc cagccaaag aatcacaat caatggaga aataatcgg aaatataag aatagccct cacaataat tataaccag tcgaccaca acaaagcgt aaaaaacaa accaaacaa tactgactt tttaatttt tatttatta ggttaaaac gcagcaggt acgcatgtt tatatgcct ggtattctg cagttgcaa ttgctgata atgacaaaa aaaatacat aatagccgc caaaagcca aaaaaagat aaaagtaat cgataagga aaatgtaac aaaaaaccg gttcttttt gttttattg tataggatt gtagtcttg aaatttctg tgttagtag gaagccatc tcatattgc tagcgttcc ataattgtt gcagtctgg agagattac attaaaatt ctacgtgtg ttcgccagt gttatgttt tatttgcat ttacagtta caactggga gtattgatg gcatatgat ggattatta tctatacgc aacaatttt atttccggg cttttattt ttaccgtct gttttttat gcaatacgt tgtggtgca atattttta tctgataat tttattaca tatcgagga tatatcaag tcaagattt tacttccat ggaaatttt tagaggaaa tagtcgcat tattaattt cattctcat ttcgagtgt gtgattgta attgtaaag gaaattgaa ttgtaaaat attgtgata taatagatt aagtgacgt ggacatgaa tcaggacag tgcaacctg tgactattt tattttatt tgttttatt gggggctga aactcgtca atttcatcg tttgcgttc aatctggtg atcatgtga atgaaaatt cttttttgt tccaattgt aaatatatt aattttgtg tttaatgca aaaacgcga tatctggcc gttttaggt ctagtgtat gtttttttt tcgaacgaa ttttgtttt tgttgggtt ctctttcaa aaattcact ttggagatt taaacgatt tgttaaatt ttgacattt ttatgtgaa gttgttact agttaggtt aagtattta tattagcgt atagtttta agagaaagt tatcgattt atgtaaaac tattaaaaa tgttttcga tttttaata ggcatagac ttaagtcag tttaagtac aaaaagaaa atgtaaaaa aaagatgtt cattacaaa aaccaaaaa aaaattttg ttacattgt acgcgccaa gatttttgt aaacatata tatttattg aaaataacg gttgaaata tattttata tcttgtg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
