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Gene information
General information
TC009265 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2196851..2202395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009265
    iBeetle screen iB_08632

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen dorsal partiallynot fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen posterior rounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009265 RA
      MDPSISWLQ PEQEGPAKH LWLRIWQTQ QELDSMNLK 
      DVDSDPEFF PNGLSNHIM NGKTDKSGS TFVQRRRKG 
      DNRASTKMM SRHPQKVVG EFGGCPWRK DPHVYARGI 
      LGLHQEIDH FYQYMSPTR TEHLVRDDV VCRIKQIIL 
      SKWPEAQVE VFGSYRTGL YLPTSDIDL VVIGKWSNL 
      PLRTLEQEF LDNDVAQEN SIKVLDKAS VPIVKLTDK 
      QTEIKVDIS FNMSNGVKS AELIKTYIH QFPVLPKLV 
      YVLKQFLLE RDLNEVFTG GISSYSLIL MCISFLQLH 
      PRPEMFVRG DANLGVFLI EFFELYGRK FNYMHTGIR 
      IRDGGRYIS KEEMQKDMV DGHRPSLLC IEDPLLPSN 
      DIGRSSYGV LQVKRAFEY AYTVLTNAV LPISSHNVE 
      HSILGRIIR VTDKVIQYR RWIEKTFGH KYNSPTIPR 
      PRPPRSLSS SGSTSSVEE SGGSSEGSD PPSRDISPN 
      NMHVQRHIQ PKNHNQWRN NRKYKNSPH NNYNQSTTT 
      KRKKQTKQY
    CDS sequence
    • >TC009265 RA
      atggatcct tcgatatct tggttgcaa ccggagcaa 
      gagggtcct gccaaacat ttgtggctt cgaatttgg 
      caaacacaa caggagctg gacagcatg aacttgaaa 
      gacgtcgac agcgacccg gagttcttc cccaacggc 
      ctctccaac cacatcatg aacggcaag acggacaag 
      agcggctcc actttcgtt cagcggaga cgtaagggc 
      gacaatcgc gccagcacc aagatgatg agccgccat 
      ccgcagaag gtggtgggc gagttcggg ggctgtccg 
      tggcgcaag gacccgcac gtctacgcc agaggcatc 
      ctgggtctg catcaggaa atcgaccac ttttaccag 
      tacatgagc cccacaagg accgaacac ctcgtcagg 
      gatgacgtc gtctgtagg atcaaacaa atcattctc 
      tccaaatgg ccagaagca caagttgaa gttttcgga 
      tcttacagg accgggctc tatcttccg acaagcgac 
      atagacttg gttgttata ggcaaatgg tcaaacctt 
      ccccttcgg acgctggaa caggaattc ctagacaac 
      gacgtggcg caagagaac agcataaaa gtcctcgac 
      aaagcgtca gtgccgatc gtgaaatta accgacaaa 
      cagaccgaa atcaaagtg gacatttcc ttcaatatg 
      tcgaacggt gtaaaatcg gccgaactg ataaaaact 
      tacatacat cagtttcca gtgttgccg aaattagtt 
      tacgtttta aaacagttc cttttagaa agagactta 
      aacgaagtg ttcaccggt ggtatatct agttacagt 
      ttaatattg atgtgcata agtttcctt cagttgcat 
      ccacgaccg gaaatgttt gtcagaggc gacgctaat 
      ttaggcgtt ttccttatt gaatttttc gaattgtac 
      ggtagaaaa tttaattat atgcatacc ggaattcgg 
      atacgagac ggcggacgg tacattagc aaagaagaa 
      atgcaaaag gacatggtg gacggacac aggcctagt 
      cttttgtgc atcgaagat ccactcttg ccatccaac 
      gacatcggc agaagcagc tacggcgtc ctgcaggtg 
      aaacgggct ttcgagtac gcgtacact gttttaacg 
      aatgccgtc cttccaatc tcgtcacac aacgtggag 
      cactcgata ttaggtaga ataataaga gtaaccgac 
      aaagtgatc caataccgg cgatggatc gaaaaaact 
      tttggtcat aagtacaac tctccgacg atcccccga 
      ccaaggcct cctcgttcc ctgtccagc agcggctcc 
      acatcgagc gttgaggaa agtgggggc agttcagaa 
      gggtccgat cctccttcg cgcgacata tcaccgaac 
      aacatgcat gtccaacgc cacatccag ccaaagaat 
      cacaatcaa tggagaaat aatcggaaa tataagaat 
      agccctcac aataattat aaccagtcg accacaaca 
      aagcgtaaa aaacaaacc aaacaatac tga
    mRNA sequence
    • >TC009265 RA
      cgagttgaa atttgcgac agtggtcgc gaaactggt 
      ttcgcttga atctggttt ggttcggtg ggtggttta 
      aaaccgtgg gatggtttg gtggtcgct ggggcgctt 
      agcaccgct cgtgttgga caagtgaca gcgcgagga 
      gtgacgttt cggtggtgt ttgtgtgta tttttgtga 
      gttttgggc gcttttttg aaattggcg ccacgtcag 
      tcgggtgtc gggcgcgct atctaccga gcatgcgag 
      acacctgtc agtttttgg tcgtgtgat atgcacaag 
      gtgtgactt cagcgtgtg ttttaatta aaattgatg 
      taataaata aaaatagcc gtatggtga agaaatcat 
      aaactatct gtgattgtt tgaatggat ccttcgata 
      tcttggttg caaccggag caagagggt cctgccaaa 
      catttgtgg cttcgaatt tggcaaaca caacaggag 
      ctggacagc atgaacttg aaagacgtc gacagcgac 
      ccggagttc ttccccaac ggcctctcc aaccacatc 
      atgaacggc aagacggac aagagcggc tccactttc 
      gttcagcgg agacgtaag ggcgacaat cgcgccagc 
      accaagatg atgagccgc catccgcag aaggtggtg 
      ggcgagttc gggggctgt ccgtggcgc aaggacccg 
      cacgtctac gccagaggc atcctgggt ctgcatcag 
      gaaatcgac cacttttac cagtacatg agccccaca 
      aggaccgaa cacctcgtc agggatgac gtcgtctgt 
      aggatcaaa caaatcatt ctctccaaa tggccagaa 
      gcacaagtt gaagttttc ggatcttac aggaccggg 
      ctctatctt ccgacaagc gacatagac ttggttgtt 
      ataggcaaa tggtcaaac cttcccctt cggacgctg 
      gaacaggaa ttcctagac aacgacgtg gcgcaagag 
      aacagcata aaagtcctc gacaaagcg tcagtgccg 
      atcgtgaaa ttaaccgac aaacagacc gaaatcaaa 
      gtggacatt tccttcaat atgtcgaac ggtgtaaaa 
      tcggccgaa ctgataaaa acttacata catcagttt 
      ccagtgttg ccgaaatta gtttacgtt ttaaaacag 
      ttcctttta gaaagagac ttaaacgaa gtgttcacc 
      ggtggtata tctagttac agtttaata ttgatgtgc 
      ataagtttc cttcagttg catccacga ccggaaatg 
      tttgtcaga ggcgacgct aatttaggc gttttcctt 
      attgaattt ttcgaattg tacggtaga aaatttaat 
      tatatgcat accggaatt cggatacga gacggcgga 
      cggtacatt agcaaagaa gaaatgcaa aaggacatg 
      gtggacgga cacaggcct agtcttttg tgcatcgaa 
      gatccactc ttgccatcc aacgacatc ggcagaagc 
      agctacggc gtcctgcag gtgaaacgg gctttcgag 
      tacgcgtac actgtttta acgaatgcc gtccttcca 
      atctcgtca cacaacgtg gagcactcg atattaggt 
      agaataata agagtaacc gacaaagtg atccaatac 
      cggcgatgg atcgaaaaa acttttggt cataagtac 
      aactctccg acgatcccc cgaccaagg cctcctcgt 
      tccctgtcc agcagcggc tccacatcg agcgttgag 
      gaaagtggg ggcagttca gaagggtcc gatcctcct 
      tcgcgcgac atatcaccg aacaacatg catgtccaa 
      cgccacatc cagccaaag aatcacaat caatggaga 
      aataatcgg aaatataag aatagccct cacaataat 
      tataaccag tcgaccaca acaaagcgt aaaaaacaa 
      accaaacaa tactgactt tttaatttt tatttatta 
      ggttaaaac gcagcaggt acgcatgtt tatatgcct 
      ggtattctg cagttgcaa ttgctgata atgacaaaa 
      aaaatacat aatagccgc caaaagcca aaaaaagat 
      aaaagtaat cgataagga aaatgtaac aaaaaaccg 
      gttcttttt gttttattg tataggatt gtagtcttg 
      aaatttctg tgttagtag gaagccatc tcatattgc 
      tagcgttcc ataattgtt gcagtctgg agagattac 
      attaaaatt ctacgtgtg ttcgccagt gttatgttt 
      tatttgcat ttacagtta caactggga gtattgatg 
      gcatatgat ggattatta tctatacgc aacaatttt 
      atttccggg cttttattt ttaccgtct gttttttat 
      gcaatacgt tgtggtgca atattttta tctgataat 
      tttattaca tatcgagga tatatcaag tcaagattt 
      tacttccat ggaaatttt tagaggaaa tagtcgcat 
      tattaattt cattctcat ttcgagtgt gtgattgta 
      attgtaaag gaaattgaa ttgtaaaat attgtgata 
      taatagatt aagtgacgt ggacatgaa tcaggacag 
      tgcaacctg tgactattt tattttatt tgttttatt 
      gggggctga aactcgtca atttcatcg tttgcgttc 
      aatctggtg atcatgtga atgaaaatt cttttttgt 
      tccaattgt aaatatatt aattttgtg tttaatgca 
      aaaacgcga tatctggcc gttttaggt ctagtgtat 
      gtttttttt tcgaacgaa ttttgtttt tgttgggtt 
      ctctttcaa aaattcact ttggagatt taaacgatt 
      tgttaaatt ttgacattt ttatgtgaa gttgttact 
      agttaggtt aagtattta tattagcgt atagtttta 
      agagaaagt tatcgattt atgtaaaac tattaaaaa 
      tgttttcga tttttaata ggcatagac ttaagtcag 
      tttaagtac aaaaagaaa atgtaaaaa aaagatgtt 
      cattacaaa aaccaaaaa aaaattttg ttacattgt 
      acgcgccaa gatttttgt aaacatata tatttattg 
      aaaataacg gttgaaata tattttata tcttgtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY