Status
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Gene information
General information
TC009282 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1938016..1942373
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009282
    iBeetle screen iB_08630

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009282 RA
      MFHALKLIP LFCTFLISP TLQTKCVSV RNNATGIEW 
      PSTRNRALS RPICLNQNQ EFVYRTCVN GNWDSEPFC 
      YYTQPEKLP ECPDGFNET ETTCYKLSE KSEYPPKCD 
      YPQTLPFSF YKEIVDLRI RVPVWVPAS RDLHNGLGL 
      VQWTEPSNY YKTEVSYKI AKSAKHHCV TYYNKTFTL 
      TSCQEEHFG VCAYLKLNQ LVDNVCESD FYCLQGDFN 
      DTSKCICKR STMVWVSYP VNYPQAEFP KPYQYSVYG 
      RLTNETCAM GLVKSPNGR YIWRNSGQE LEYTFWSKN 
      VDFTKSHQG YAAATPEGW ILTRGPTRC ALFELEVKD 
      LEPSLSLSF NPSTNKFLL EVANSKRVK FLYFSALIF 
      CFTDADLTL VYRYPALNA LSTGERLVL QFDPFPLPG 
      NYWCEAFKF VDLGVIQTI YFHRPVVYP EFVTIISVR 
      YDKYSDPLS SDFVTKLNQ SELFHSLRN NLYIQTFYQ 
      LRIMKIIDL DEDTSRVTV NVHLSSNFG NSSFNETFQ 
      YETIKHVVS QAISGLKTE QIDLVDFLS SDFCPNESN 
      RLTWPQTPV NSTASPDEF CLTSNGSFV TRWCLGDFV 
      DGAKWLHYE NCVIYPKSN ITRQLLMIM NYSKSSLLD 
      TLFNLSANY REFEPVDVY LFGEMFNGL DNITSGDLV 
      LFAKTVNNL LKIDRKIAR QAQLELGAS DKFLELIEN 
      FTRQCKDSS ETWLNNFVL VSVESGYLS GLVVEKQDE 
      QIVVRKLTD DHSVPELTK SQNLEYAIW LSPKTRQKI 
      PHDEKVTFW LFLDDSFFQ QTVPGFRTG PVFGILHPE 
      GVTFTADAV RVLRRVTPN QKVDFCGKW SYSSHNRGF 
      WERSGQIKD YEPFVLCEF GEAANFGLV SFNDDNVTD 
      DLIDLLDSN DTVTETLDK LSHISERYE QFKPIDVAI 
      VGQILGKVN YDEEIDLEI LAVTVSNLH EIDRGILSD 
      SQRQDRSTD SILANVDII LQNQKIADK VQITAQNFI 
      VFIAYFTKT FNGLVLLKH EGVLEPIIL EGNLTIDDV 
      AKYNCFDSA VVLSPELKK QISEKTKII ITVFFNDAL 
      FNEKTNNNK VSNKIFGVI IVPPLGKYH GPVSIYHKL 
      SETVLRDCV FWNYSHNIP GSWQNDTKA QNDQNLTRC 
      DFWHTTHFG QLLLEGDKF RSSIEDSDI LSIITNINC 
      MVSLIGLAF IILTAVIFK RWRENTGNQ ILLNFTFVI 
      IVQIAIFYI SNSVNAFHK DYVFCTIIG VSLHYSIIS 
      QFCWMLVIA VLQFRRFVT VLEGPPRYI LLKASISGW 
      VLPLFPVLA VFISDESNY VNSSAGLCY PSGLGLYLG 
      VWLPVALIV LINSVIFTV TLYNVVHKK TEVVFNGHN 
      EALYQWRLA VMLFFMLGL TWCFGFLAK LDVGLVFEY 
      LFCITATLQ GFTIFLFFI VFNVNTRYL YIGVINKYC 
      NKINN
    CDS sequence
    • >TC009282 RA
      atgttccac gccctgaaa ctaatcccc cttttctgc 
      acgttccta atctcccca actctccaa acgaaatgc 
      gtaagcgtc cgaaacaac gcaacagga atcgaatgg 
      cccagcacc cgaaaccgt gctttatcg cgccccatt 
      tgcctaaac caaaaccag gagtttgtc tacagaacc 
      tgcgtgaac ggtaattgg gacagcgag cctttttgc 
      tactacact cagcctgag aaactgcca gaatgtcca 
      gacgggttc aacgaaact gaaacgact tgctacaag 
      ttgagcgaa aaatccgag taccccccc aagtgcgac 
      taccctcaa actttaccg ttttctttc tacaaggaa 
      atcgtagat ttgaggatc cgtgtcccg gtttgggtc 
      cccgcaagt cgggatttg cacaacggg ctgggttta 
      gtccagtgg actgaaccc agcaactac tacaagact 
      gaagtctcg tacaaaatc gcaaaatcg gccaaacac 
      cactgtgtc acttactac aacaaaacc ttcacgctg 
      acttcctgc caggaggaa cactttggt gtttgtgcg 
      tatttgaag ctcaaccaa cttgtggac aacgtgtgc 
      gagtcggat ttctactgc cttcaagga gatttcaac 
      gacacgtcc aagtgcatt tgcaaaaga tcgaccatg 
      gtgtgggtc agctaccca gtgaactac ccccaagcc 
      gagttcccc aagccctac cagtacagc gtttatggg 
      cgtttgacc aacgagact tgtgccatg ggtttggtc 
      aaaagccca aatgggcgc tacatttgg cgcaattct 
      ggccaagaa ctcgagtac actttttgg tcaaaaaac 
      gttgatttc accaagagc catcaaggg tacgcagct 
      gcgaccccc gaaggctgg attttgacc agaggaccc 
      actcgttgt gctttattt gagctggaa gtcaaagac 
      ttggaaccg agtctatcg ctttctttt aacccaagt 
      accaacaaa tttttacta gaggtggcc aactcaaaa 
      agagtcaag tttttgtat ttcagtgcc cttatattt 
      tgcttcact gatgccgat ttgaccttg gtttatcgt 
      taccctgct ctaaatgct ctttcaaca ggggaaaga 
      ctagttctc cagtttgat ccgttcccc cttccgggg 
      aactactgg tgcgaagcc ttcaaattt gtggacttg 
      ggcgtaatt caaacgatt tattttcac agaccggtg 
      gtttacccc gaatttgtg acaatcata tcagtgcgt 
      tacgacaag tattctgac cccttgagc agtgatttt 
      gtcacaaag ctaaatcag agtgagttg ttccactcc 
      ctgaggaac aatttgtac atccagacg ttctaccag 
      ttaagaatc atgaaaatt attgatctg gatgaagac 
      acaagtcga gtgacagtc aacgtacat ttgagttct 
      aattttggg aacagctca ttcaatgaa acgtttcaa 
      tacgaaact attaaacac gtcgtgagc caagctatc 
      agtggtctt aagacagaa caaatcgat ttggtggat 
      ttcctaagt tccgacttt tgccccaac gaatcaaac 
      cgcctgaca tggccccaa acacccgtc aactccact 
      gcaagtccg gacgaattt tgcctcact tctaatgga 
      agttttgtg acacgctgg tgtcttggt gatttcgta 
      gacggtgcc aagtggcta cactacgaa aattgtgtc 
      atttatcca aaatcaaac atcacaaga cagttatta 
      atgatcatg aactactcc aagagttcg cttctagac 
      acacttttc aatctttcc gcaaactat cgagagttt 
      gaacccgtg gatgtgtac ctctttggc gagatgttc 
      aacggtttg gacaatatc acgtccgga gatcttgtc 
      ctgtttgca aaaaccgta aacaatctt ctgaaaatc 
      gacaggaag attgcacgg caagcccag cttgaacta 
      ggggccagt gataagttt ctggagctg attgaaaat 
      tttaccaga caatgtaag gactcgtct gagacttgg 
      ctgaataat ttcgtgttg gtttcggtc gagtctggc 
      tatttgagt ggcctggtt gtggaaaaa caggacgag 
      caaatagtt gtgcgtaaa ctgacagat gaccactca 
      gtgcccgaa ttgaccaaa agccaaaac ctggaatat 
      gccatttgg ttgagtccc aaaacgcgc caaaaaatc 
      ccccatgac gaaaaagtc acgttttgg ctctttctc 
      gacgattcg ttcttccag caaactgtt cccggtttt 
      cggacgggt ccagttttc gggattctg catccagaa 
      ggtgtgact ttcactgcc gacgctgtg agagtgtta 
      cgtcgtgtg acaccgaac cagaaagtt gatttttgt 
      ggcaaatgg agctactca tcacacaat cgcggtttt 
      tgggagaga agtggacaa attaaggat tatgaaccg 
      tttgtattg tgtgagttt ggcgaagct gcgaatttt 
      ggcttggtt tctttcaat gatgacaat gtgactgat 
      gatctgatc gatttgctt gactcgaat gacactgtg 
      accgaaact ttggataag ctttcgcac atttcggag 
      cggtatgag cagttcaaa ccaattgat gtggcaatt 
      gtggggcaa atcttgggg aaagttaac tatgacgag 
      gaaattgat ttggagatt ttggcggtt actgttagc 
      aatttgcac gaaatcgac cgcgggatt ttatcagac 
      agtcaacgc caagacagg tccactgat tcgatcctt 
      gccaacgtc gacataatc ctccaaaac cagaaaatt 
      gcagacaaa gtacaaata acagctcaa aattttata 
      gtttttatc gcctatttt acaaaaact ttcaatggt 
      ttagtgttg ctaaagcat gaaggtgtt ttggaaccg 
      atcatcctg gaggggaac ttgaccatt gacgacgtg 
      gccaagtat aactgtttt gacagcgcg gttgttctt 
      agcccagag ctgaaaaaa caaatttcg gaaaagaca 
      aaaatcata attacggtg tttttcaac gacgcactt 
      tttaacgaa aaaacaaac aacaacaaa gtgtctaat 
      aaaattttc ggagtcatt atagttccc ccactgggg 
      aaataccac ggtccagtt tcgatctac cataaactt 
      tcggagaca gttttgagg gattgtgtt ttttggaat 
      tactcgcac aatattccc ggctcttgg caaaacgac 
      accaaggcc caaaacgac caaaacctg actcggtgc 
      gatttctgg cacacaaca cacttcggg caactgctc 
      ctggaaggc gacaagttc aggagcagt attgaggac 
      tccgacatc ctcagcatc atcacaaac atcaactgc 
      atggtttct ctaatcggc ctcgctttc attatcctc 
      acagcggtg attttcaag cgctggcgc gaaaacacc 
      ggcaaccaa atcttgctc aacttcacc ttcgtaata 
      atcgtccaa attgcaatt ttctacatt tcaaactcg 
      gtaaatgcg ttccataaa gactacgtt ttctgcacc 
      atcattggc gtctccttg cactactcc atcatctcg 
      cagttttgc tggatgttg gtgattgca gttttgcag 
      tttcggcgc tttgtcacc gtcttggaa ggaccaccg 
      agatacatc ttgctcaaa gcgtccatc tctggctgg 
      gttctaccc ttgtttcca gtcctggcg gtttttatc 
      tcagacgaa agcaactac gttaatagc agtgccggg 
      ctgtgctac ccgtcaggc ctggggctg tatctgggg 
      gtttggctg ccagtggcg ctaatagtt ttgataaat 
      tcggtcatt ttcactgta actttgtac aatgtggtt 
      cataaaaag accgaagtt gttttcaac ggtcacaat 
      gaagcgctg tatcagtgg agattggcg gtgatgctg 
      ttttttatg ctgggtttg acttggtgt ttcggcttt 
      cttgcaaaa ttagacgtt ggactagtc tttgagtat 
      ttgttttgc atcacggcc actcttcaa gggtttacc 
      atatttctc ttcttcatt gtgttcaat gtcaacacc 
      aggtacttg tacattggc gtgatcaac aagtattgc 
      aataaaatc aataactaa
    mRNA sequence
    • >TC009282 RA
      ttagttttt tctggctac atgatcaat acgatgttc 
      cacgccctg aaactaatc ccccttttc tgcacgttc 
      ctaatctcc ccaactctc caaacgaaa tgcgtaagc 
      gtccgaaac aacgcaaca ggaatcgaa tggcccagc 
      acccgaaac cgtgcttta tcgcgcccc atttgccta 
      aaccaaaac caggagttt gtctacaga acctgcgtg 
      aacggtaat tgggacagc gagcctttt tgctactac 
      actcagcct gagaaactg ccagaatgt ccagacggg 
      ttcaacgaa actgaaacg acttgctac aagttgagc 
      gaaaaatcc gagtacccc cccaagtgc gactaccct 
      caaacttta ccgttttct ttctacaag gaaatcgta 
      gatttgagg atccgtgtc ccggtttgg gtccccgca 
      agtcgggat ttgcacaac gggctgggt ttagtccag 
      tggactgaa cccagcaac tactacaag actgaagtc 
      tcgtacaaa atcgcaaaa tcggccaaa caccactgt 
      gtcacttac tacaacaaa accttcacg ctgacttcc 
      tgccaggag gaacacttt ggtgtttgt gcgtatttg 
      aagctcaac caacttgtg gacaacgtg tgcgagtcg 
      gatttctac tgccttcaa ggagatttc aacgacacg 
      tccaagtgc atttgcaaa agatcgacc atggtgtgg 
      gtcagctac ccagtgaac tacccccaa gccgagttc 
      cccaagccc taccagtac agcgtttat gggcgtttg 
      accaacgag acttgtgcc atgggtttg gtcaaaagc 
      ccaaatggg cgctacatt tggcgcaat tctggccaa 
      gaactcgag tacactttt tggtcaaaa aacgttgat 
      ttcaccaag agccatcaa gggtacgca gctgcgacc 
      cccgaaggc tggattttg accagagga cccactcgt 
      tgtgcttta tttgagctg gaagtcaaa gacttggaa 
      ccgagtcta tcgctttct tttaaccca agtaccaac 
      aaattttta ctagaggtg gccaactca aaaagagtc 
      aagtttttg tatttcagt gcccttata ttttgcttc 
      actgatgcc gatttgacc ttggtttat cgttaccct 
      gctctaaat gctctttca acaggggaa agactagtt 
      ctccagttt gatccgttc ccccttccg gggaactac 
      tggtgcgaa gccttcaaa tttgtggac ttgggcgta 
      attcaaacg atttatttt cacagaccg gtggtttac 
      cccgaattt gtgacaatc atatcagtg cgttacgac 
      aagtattct gaccccttg agcagtgat tttgtcaca 
      aagctaaat cagagtgag ttgttccac tccctgagg 
      aacaatttg tacatccag acgttctac cagttaaga 
      atcatgaaa attattgat ctggatgaa gacacaagt 
      cgagtgaca gtcaacgta catttgagt tctaatttt 
      gggaacagc tcattcaat gaaacgttt caatacgaa 
      actattaaa cacgtcgtg agccaagct atcagtggt 
      cttaagaca gaacaaatc gatttggtg gatttccta 
      agttccgac ttttgcccc aacgaatca aaccgcctg 
      acatggccc caaacaccc gtcaactcc actgcaagt 
      ccggacgaa ttttgcctc acttctaat ggaagtttt 
      gtgacacgc tggtgtctt ggtgatttc gtagacggt 
      gccaagtgg ctacactac gaaaattgt gtcatttat 
      ccaaaatca aacatcaca agacagtta ttaatgatc 
      atgaactac tccaagagt tcgcttcta gacacactt 
      ttcaatctt tccgcaaac tatcgagag tttgaaccc 
      gtggatgtg tacctcttt ggcgagatg ttcaacggt 
      ttggacaat atcacgtcc ggagatctt gtcctgttt 
      gcaaaaacc gtaaacaat cttctgaaa atcgacagg 
      aagattgca cggcaagcc cagcttgaa ctaggggcc 
      agtgataag tttctggag ctgattgaa aattttacc 
      agacaatgt aaggactcg tctgagact tggctgaat 
      aatttcgtg ttggtttcg gtcgagtct ggctatttg 
      agtggcctg gttgtggaa aaacaggac gagcaaata 
      gttgtgcgt aaactgaca gatgaccac tcagtgccc 
      gaattgacc aaaagccaa aacctggaa tatgccatt 
      tggttgagt cccaaaacg cgccaaaaa atcccccat 
      gacgaaaaa gtcacgttt tggctcttt ctcgacgat 
      tcgttcttc cagcaaact gttcccggt tttcggacg 
      ggtccagtt ttcgggatt ctgcatcca gaaggtgtg 
      actttcact gccgacgct gtgagagtg ttacgtcgt 
      gtgacaccg aaccagaaa gttgatttt tgtggcaaa 
      tggagctac tcatcacac aatcgcggt ttttgggag 
      agaagtgga caaattaag gattatgaa ccgtttgta 
      ttgtgtgag tttggcgaa gctgcgaat tttggcttg 
      gtttctttc aatgatgac aatgtgact gatgatctg 
      atcgatttg cttgactcg aatgacact gtgaccgaa 
      actttggat aagctttcg cacatttcg gagcggtat 
      gagcagttc aaaccaatt gatgtggca attgtgggg 
      caaatcttg gggaaagtt aactatgac gaggaaatt 
      gatttggag attttggcg gttactgtt agcaatttg 
      cacgaaatc gaccgcggg attttatca gacagtcaa 
      cgccaagac aggtccact gattcgatc cttgccaac 
      gtcgacata atcctccaa aaccagaaa attgcagac 
      aaagtacaa ataacagct caaaatttt atagttttt 
      atcgcctat tttacaaaa actttcaat ggtttagtg 
      ttgctaaag catgaaggt gttttggaa ccgatcatc 
      ctggagggg aacttgacc attgacgac gtggccaag 
      tataactgt tttgacagc gcggttgtt cttagccca 
      gagctgaaa aaacaaatt tcggaaaag acaaaaatc 
      ataattacg gtgtttttc aacgacgca ctttttaac 
      gaaaaaaca aacaacaac aaagtgtct aataaaatt 
      ttcggagtc attatagtt cccccactg gggaaatac 
      cacggtcca gtttcgatc taccataaa ctttcggag 
      acagttttg agggattgt gttttttgg aattactcg 
      cacaatatt cccggctct tggcaaaac gacaccaag 
      gcccaaaac gaccaaaac ctgactcgg tgcgatttc 
      tggcacaca acacacttc gggcaactg ctcctggaa 
      ggcgacaag ttcaggagc agtattgag gactccgac 
      atcctcagc atcatcaca aacatcaac tgcatggtt 
      tctctaatc ggcctcgct ttcattatc ctcacagcg 
      gtgattttc aagcgctgg cgcgaaaac accggcaac 
      caaatcttg ctcaacttc accttcgta ataatcgtc 
      caaattgca attttctac atttcaaac tcggtaaat 
      gcgttccat aaagactac gttttctgc accatcatt 
      ggcgtctcc ttgcactac tccatcatc tcgcagttt 
      tgctggatg ttggtgatt gcagttttg cagtttcgg 
      cgctttgtc accgtcttg gaaggacca ccgagatac 
      atcttgctc aaagcgtcc atctctggc tgggttcta 
      cccttgttt ccagtcctg gcggttttt atctcagac 
      gaaagcaac tacgttaat agcagtgcc gggctgtgc 
      tacccgtca ggcctgggg ctgtatctg ggggtttgg 
      ctgccagtg gcgctaata gttttgata aattcggtc 
      attttcact gtaactttg tacaatgtg gttcataaa 
      aagaccgaa gttgttttc aacggtcac aatgaagcg 
      ctgtatcag tggagattg gcggtgatg ctgtttttt 
      atgctgggt ttgacttgg tgtttcggc tttcttgca 
      aaattagac gttggacta gtctttgag tatttgttt 
      tgcatcacg gccactctt caagggttt accatattt 
      ctcttcttc attgtgttc aatgtcaac accaggtac 
      ttgtacatt ggcgtgatc aacaagtat tgcaataaa 
      atcaataac taatccaac gttcagatt gacttgggc 
      aaagctatt gtcggtact gttgacaaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY