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Gene information
General information
TC009295 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1807468..1810508
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009295
    iBeetle screen iB_08626

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009295 RA
      MLTLNVVPE RSLGCEQWE FIVGMHFSQ AIAIIQSQV 
      GNIKNVQVL YNDTNPLDI DLVLNLPQD GIRLFFDPI 
      SQRLKIIEI YCMKLVKLK YCNLPFNSP EVLPSIEQI 
      EHSFGATHP GVYDSEKEL FILNFRGLS FYFPVESKF 
      QSGNTHNLG SLQFPPGSS PLVSRMTIY YGSNIEQAC 
      APELPLSCY HGQLYLQKA EILRGECYT RGVKLSLLA 
      GSNNRRMDS KIQFDREIL LGHTAQDVA TALGAPNEV 
      FYKSEDKMR IHTPNALRR DADTKSDYF FNYFTLGLD 
      VLFDARSHL VKKLVMHTN YPGHYNFNI YMRCEFKLA 
      LNGADIDAY CHWDDICKQ LTPSERPVV LNRASSTNT 
      TNPFGSTLC YGYQDIIFE VMPNNLIAS LSVYGDGRP 
      YQSESEHA
    CDS sequence
    • >TC009295 RA
      atgctgacg ctcaacgtt gttcctgaa cgttctttg 
      ggctgtgaa cagtgggag tttattgta ggaatgcat 
      ttttcccag gctattgca attattcag tcccaagtg 
      ggcaacata aagaatgta caagtcctc tataacgat 
      actaaccct ttagacata gacctcgtt cttaattta 
      ccccaggac ggcattaga ttatttttt gaccccatt 
      tcacagcgg ctaaaaata atcgaaata tactgtatg 
      aaattagtc aagttaaaa tactgtaac ctcccattt 
      aactcgcca gaagtactg ccatcaatc gaacaaatt 
      gaacactca ttcggtgcc actcatcca ggagtatat 
      gattcggag aaggagcta ttcatattg aattttcgg 
      ggactttcg ttctatttt ccagtcgaa tcaaaattc 
      cagtctgga aacacccat aatctgggt tctctccaa 
      ttcccccca gggagttcc ccgctagtg tcacgcatg 
      acgatttac tacggctca aatatcgag caggcctgc 
      gcccccgaa ctgcccctg tcttgttac cacggccaa 
      ttgtacctc caaaaggcc gaaattttg aggggcgag 
      tgctacacc agaggagtc aagctcagc ctccttgca 
      ggctcaaac aaccgtcga atggactca aaaatccaa 
      ttcgaccgt gagatcctc ttggggcac acggcccaa 
      gacgtggcc acggcttta ggggccccc aatgaagtc 
      ttctataaa agtgaggac aaaatgcgg attcacacc 
      cccaacgcc ctgcgtcgc gacgccgac actaagagc 
      gactatttc ttcaattat ttcacgttg ggattggac 
      gtcctgttc gatgcccgt tcgcatttg gtgaaaaaa 
      ttggtcatg catacgaat tatccaggc cattacaat 
      ttcaacatt tacatgaga tgcgaattt aagttggcg 
      ctgaatggc gccgatatc gacgcctac tgccattgg 
      gacgatatc tgtaaacaa ctgacgcca agtgaacga 
      ccggttgtc ttaaatagg gctagctcc actaatacg 
      accaatccg ttcgggagc accttgtgc tatggctat 
      caggatatc atcttcgag gtgatgccc aacaacctg 
      atcgcctcc ttgagcgtg tacggtgat ggaaggccg 
      taccagagc gaatcagag catgcgtga
    mRNA sequence
    • >TC009295 RA
      gaaaatgct gacgctcaa cgttgttcc tgaacgttc 
      tttgggctg tgaacagtg ggagtttat tgtaggaat 
      gcatttttc ccaggctat tgcaattat tcagtccca 
      agtgggcaa cataaagaa tgtacaagt cctctataa 
      cgatactaa ccctttaga catagacct cgttcttaa 
      tttacccca ggacggcat tagattatt ttttgaccc 
      catttcaca gcggctaaa aataatcga aatatactg 
      tatgaaatt agtcaagtt aaaatactg taacctccc 
      atttaactc gccagaagt actgccatc aatcgaaca 
      aattgaaca ctcattcgg tgccactca tccaggagt 
      atatgattc ggagaagga gctattcat attgaattt 
      tcggggact ttcgttcta ttttccagt cgaatcaaa 
      attccagtc tggaaacac ccataatct gggttctct 
      ccaattccc cccagggag ttccccgct agtgtcacg 
      catgacgat ttactacgg ctcaaatat cgagcaggc 
      ctgcgcccc cgaactgcc cctgtcttg ttaccacgg 
      ccaattgta cctccaaaa ggccgaaat tttgagggg 
      cgagtgcta caccagagg agtcaagct cagcctcct 
      tgcaggctc aaacaaccg tcgaatgga ctcaaaaat 
      ccaattcga ccgtgagat cctcttggg gcacacggc 
      ccaagacgt ggccacggc tttaggggc ccccaatga 
      agtcttcta taaaagtga ggacaaaat gcggattca 
      cacccccaa cgccctgcg tcgcgacgc cgacactaa 
      gagcgacta tttcttcaa ttatttcac gttgggatt 
      ggacgtcct gttcgatgc ccgttcgca tttggtgaa 
      aaaattggt catgcatac gaattatcc aggccatta 
      caatttcaa catttacat gagatgcga atttaagtt 
      ggcgctgaa tggcgccga tatcgacgc ctactgcca 
      ttgggacga tatctgtaa acaactgac gccaagtga 
      acgaccggt tgtcttaaa tagggctag ctccactaa 
      tacgaccaa tccgttcgg gagcacctt gtgctatgg 
      ctatcagga tatcatctt cgaggtgat gcccaacaa 
      cctgatcgc ctccttgag cgtgtacgg tgatggaag 
      gccgtacca gagcgaatc agagcatgc gtgacagag 
      gctctctgc cacttacct cattctggc aaacaggta 
      ctggcaacc gtaagaatc taatcccct acatcccta 
      gtatacaac atgtacagt taacgtcaa tatctagta 
      acggattta tagcttaat tgtaaggtt aattactct 
      agttaattg aaaaaatta accaaaaaa aattcaaaa 
      gtgtgatga aaaccaaaa aaattcaat aaatgaaaa 
      aattggtaa aaaaattgc aagccctgg ctagtataa 
      ttagctttg tgaaattgt gagcttaaa gagcgcagg 
      gtgatgtgt tcaatttcg tttttgcca tttggaagt 
      ttccctgta aatatattt caacttttg tccacacaa 
      taataatgg tttcaaaac caaagaaaa atgtaaaaa 
      gtttttata ggacgttgc gtttcagtt tgtgagcgt 
      ttttattaa gcaataagt gtgtagtaa tttatgttt 
      aatttatgt aacggatca cctgagcgg tatttaatt 
      tgcgcatta ctgcagtaa tgtttttcc aattcagtt 
      tgtacatta ttgtatcaa taaatatta ccgtaagtt 
      agtcatact gatctggtt tgttgcttc ttttcgaaa 
      tcaacggcc ggacttgca ttcggtgca tttgtttcg 
      tgatatgac gccagtaca tcggggatt cccacaggt 
      ctcccgaca cgcatgcaa gtccgccgt tcgtgtgac 
      caactgttt atatttatt tatgtgatt acgctcttg 
      agacagtat atttttata tttaatcat aatcatttc 
      agatctcat aaggcttta gttttaaaa caaaataaa 
      aatgtgatt catgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY