General information | |||
TC009298 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 1781680..1783841 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC009298 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_08625 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
abdominal odoriferous glands content opaque - Number of animals showing the phenotype: 5 (13 dpi)
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
mandible not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs podomer partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC009298 RA
MTNNKPNCA CLFPFLPCW YPLSPDDLI KKRRSNDID KQIAKDKEE FNKTLRLLL LGAGESGKS TIIKQMQLI HVDKFSDTV RKQRRDDIR DNLADAIVS ILGAMPKIT PPVRLDNER HETMAKWVL DNATKQDFD YPSQFYEFT KILWKDTGV QATFERSNE YQLVDSAKY FLEKIDEIA RDDYMPTDQ DIIRCRVLT TGIYELSFQ VKEINFQII DVGGQRDER RKWFLCFND VTAIIFVTA CSSYNLVLR EDPSKNRLK ESLELFKIV WNNRWLCRI SVIVFLNKQ DLFREKILS GRSKLEDYF PEYKDFKTS HVERDEHPE VNRAKHFIK NEFEKISRL SNQSLQHRF YPHFTCAVD TDDVKRVFD VCQTVFDDC RKNIQIKHL IEHDLM
- >TC009298 RA
atgactaac aataaacca aactgcgcg tgtttgttt ccattcttg ccctgttgg taccccctg agccccgac gatttgatc aagaagcgg cgcagcaat gacatcgac aagcagatt gctaaagac aaggaggag tttaataaa accctccgc ttgttactg ttgggggcc ggggagtca ggcaagtcc accatcatc aaacagatg cagcttatc cacgtggat aagttcagt gatacggtt aggaagcaa agacgggac gatattaga gacaatctg gctgatgcg atagtgagc atacttggg gccatgccc aaaatcact ccgccagtc agattagat aatgagagg catgagaca atggcaaag tgggtgcta gataacgca actaagcag gattttgat tatccgagc cagttttac gagtttacg aaaattttg tggaaggat acaggcgtt caggccact tttgagagg agtaacgaa tatcagttg gtagacagt gctaagtat tttttggaa aagatcgac gaaattgca agggatgat tacatgccc actgatcag gatattata agatgcagg gtcctaact acgggtatt tacgaactc tccttccaa gtcaaggaa attaatttc caaataatc gacgttggg gggcagcgg gacgagcgc cgcaagtgg ttcttgtgc ttcaatgac gtcacggcg attatcttc gtcacagct tgtagttcc tataatttg gttttgcgc gaagacccg tccaaaaac cgcctcaaa gagagcctc gaattgttc aaaatcgtg tggaacaac cgctggttg tgccgcatt tccgtgata gtgttctta aataagcag gatctcttc agggaaaaa atcctctca gggcgcagt aaactcgag gactacttt cccgaatat aaagacttc aagacgtcg cacgtggaa cgcgacgag catcccgag gtgaatcgg gccaaacat tttataaaa aacgagttt gaaaaaatt agccgattg agcaatcaa tcgttacag caccggttt tacccccat ttcacgtgt gcggtagac actgacgac gttaagagg gtctttgat gtgtgtcaa acggtgttt gacgactgt cgcaaaaac atccaaatc aagcatctc atcgaacac gatttaatg taa
- >TC009298 RA
gccacccaa cgtcaacca actgtcacg ttgacacgt cgcctaacc caacttatt tgtttatga ttttcggct tgtttttcg cggtgattc gactagatt taagttcag gatgtacta tgactaaca ataaaccaa actgcgcgt gtttgtttc cattcttgc cctgttggt accccctga gccccgacg atttgatca agaagcggc gcagcaatg acatcgaca agcagattg ctaaagaca aggaggagt ttaataaaa ccctccgct tgttactgt tgggggccg gggagtcag gcaagtcca ccatcatca aacagatgc agcttatcc acgtggata agttcagtg atacggtta ggaagcaaa gacgggacg atattagag acaatctgg ctgatgcga tagtgagca tacttgggg ccatgccca aaatcactc cgccagtca gattagata atgagaggc atgagacaa tggcaaagt gggtgctag ataacgcaa ctaagcagg attttgatt atccgagcc agttttacg agtttacga aaattttgt ggaaggata caggcgttc aggccactt ttgagagga gtaacgaat atcagttgg tagacagtg ctaagtatt ttttggaaa agatcgacg aaattgcaa gggatgatt acatgccca ctgatcagg atattataa gatgcaggg tcctaacta cgggtattt acgaactct ccttccaag tcaaggaaa ttaatttcc aaataatcg acgttgggg ggcagcggg acgagcgcc gcaagtggt tcttgtgct tcaatgacg tcacggcga ttatcttcg tcacagctt gtagttcct ataatttgg ttttgcgcg aagacccgt ccaaaaacc gcctcaaag agagcctcg aattgttca aaatcgtgt ggaacaacc gctggttgt gccgcattt ccgtgatag tgttcttaa ataagcagg atctcttca gggaaaaaa tcctctcag ggcgcagta aactcgagg actactttc ccgaatata aagacttca agacgtcgc acgtggaac gcgacgagc atcccgagg tgaatcggg ccaaacatt ttataaaaa acgagtttg aaaaaatta gccgattga gcaatcaat cgttacagc accggtttt acccccatt tcacgtgtg cggtagaca ctgacgacg ttaagaggg tctttgatg tgtgtcaaa cggtgtttg acgactgtc gcaaaaaca tccaaatca agcatctca tcgaacacg atttaatgt aactgtggt acttatgca agcatttat ttttgttat ctattgtag tttactttt atgttctca aagaacgaa aactagtct agtcggttt ctgattcag tatttgtat gtgatatag cgagagtcg aaataaaac gcggtcaag agtgctgtg acattatgt aaattaaac aattggtat atttgacag attattgat aggtgttga cgttcatgt catagcacc cttgaccac gcttacttg ttaaaacga atttttatg cttcctcca gtggcgcct cggcagttc aaaactagg caaaattgt atcaaaatt gataaaaat caatcagtt aactacctg gaaaattac gttattttg aggtacaaa cccaagaag gagaaaaga ttcattact attcattaa ttttaggtg gagtcactt cacctattt gattaagcg agtcgccac tggcttttt tagttttca tcctgttat tcgtgcctt tacacgaac taaaatctg gacaaaatt atttgtgcc ttaatttat ttttttatt gttttctct aataaagat ctgtctgat cttaatctc ccagtagtt gagtatctc acactgtaa taaataagt caataaatt aactgcggc taa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
